지점부 다이아프램의 응력분배 거동

김동표,정경섭,김경남,양경환 충북대학교 건설기술연구소 2002 建設技術論文集 Vol.21 No.1

These days, bridges have a tendency to be long-spanned and simple ‘ thus, the importance of economy and efficiency has increased for bridge construction. As a result, one of the main members on which interest is focused is the diaphragms. In this study, stress variation of the support diaphragms in a steel box girder was considered. This paper deals with the behavior of the stress distribution on the diaphragms on the bearings of a closed section steel box girder bridge due to the continuous casting of concrete slab. Both experimental and structural analyses were performed to verify the behavior of the stress distribution a composite steel box girder bridge under construction. Strain was continuously checked at intervals of 10 to 30 min. from the beginning of the placement by the Time Interval Function of the measuring instrument. The variation of strain on the diaphragms on the bearings are presented herein.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

有鉤囊尾蟲症 診斷에 있어서 特異 抗原帶의 증명에 관한 硏究

朱炅煥,姜星鎬,李駿商,林漢鍾 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.3

Human cysticercosis caused by the larval stages of cestode Taenia solium is important public health problem in many of the developing countries including Korea. The application of computerized axial tomography has greatly assisted the diagnosis of neurocysticercosis. However many cases require serological examination for diagnostic confirmation. But there are some limiting factors in seroimmunological diagnosis for the detection of antibodies in serum or cerebrospinal fluid. One of the major problems is the non-specificity of the antigens which produce cross reaction with antibodies produced by the presence of other helminthic infections. Especially the sensitive tests such as ELISA are highly dependant on the purity of antigens used. To solve this problem many workers have tried to find species specific components of the antigens. Accordingly, the present studies were performed to determine the antigenic profile of the vesicular fluid and parenchymal antigens of metacestodes of Taenia solium based on SDS-PAGE and immunoblotting techniques. Analysis of human serum reactivities to these antigens gave the following results. 1. The SDS-PAGE of CyF₁ (vesicular fluid antigen) showed protein bands at the 116-200K, 106K, 91K, 63K, 21K and 2 faint bands at 97K, and 80K molecular weight region. Among them first 2 masses of bands were non-specific and the EITB patterns showed species specific reactive bands at the 91K, 63K, and 21K region. 2. The SDS-PAGE of CyL₁ (larval antigen) showed protein bands at the 116-200K, 106K, 91K, 80K, 63K, 43K, 31K, 21K, 14K and many faint bands. Among them, 3 bands at the region of 63K, 43K, and 14K showed species specific reactions. 3. The antibody produced by P. westermani, Sparganum and D. latum infected patients showed non-specific cross reaction with 116-200K, and 106K bands of CyF₁ and all bands of 80K, 116K and 116-200K bands of CyL₁. 4. The band at the region of 63K of both antigen showed strong positive reaction without interference and cross reaction of other helminthic infectons. Serum of nine Cysticercus infected patients examined showed positive reaction at the band of 63K without exception. The high species-specificity demonstrated by the present methods could be used to confirm serologically an infection by Taenia solium metacestodes.

지르코늄―나이트렌 화합물 합성

차경환,김의진,이순원 성균관대학교 기초과학연구소 1993 論文集 Vol.44 No.2

Zr(acac)Cl_3(THF) 및 Zr(acac)_2Cl_2(acac=acetylactate, CH_3COCHCOCH_3^1) 화합물이 [Li(THF)_x][PhNN=NNPh], Ⅰ, 리간드와 반응하여 각각 Zr(=NPh)(acac)CI(THF), Ⅱ, 및 Zr(=NPh)(acac)_2, Ⅲ, 착화합물을 생성하였다. 이들을 ^1H-NMR, FTIR 스펙트럼으로 분석하였다. Reactions between Zr(acac)CI_3(THF) and Zr(acac)_2Cl_2(acac=CH_3 COCHCOCH_3^1) with [Li(THF)_x][PhNN=NNPh], Ⅰ, led to Zr(=NPh)(acac) Cl(THF), Ⅱ, and Zr(=NPh)(acac)_2, Ⅲ, respectively. They have been characterized by ^1H-NMR and FTIR spectroscopy.

초등학교 고학년 학생의 과학 탐구능력 측정을 위한 평가 도구 개발

송경혜,이항로,임청환 한국과학교육학회 2004 한국과학교육학회지 Vol.24 No.6

본 연구에서는 평가도구의 구비조건을 갖춘 초등학교 고학년 학생의 과학탐구능력을 측정할 수 있는 과학탐구 능력 평가도구를 개발하고자 하였다. 평가도구를 구성하는 문항은 R&D 방법에 의해 개발되었으며, 2차례에 걸친 과학교육 전문가의 점검과 2번의 현장 검증을 통해 문항을 수정ㆍ보완하였다. 각 평가 문항은 교과 내용에 대한 지식이 없어도 해결할 수 있는 범교과적인 문항으로 제작하여 과학탐구능력의 하위 요소들을 측정하고자 하였다. SAPA 교육과정과 제7차 과학과 교육과정에서 제시한 과학탐구과정 요소를 근간으로 관찰, 분류, 측정, 예상, 추리, 문제인식, 변인통제, 자료해석, 결론도출, 실험설계의 10가지 하위 요소들을 선정하고 각 요소에 대한 조작적 정의와 평가 목표를 설정하였다. 물질과 에너지, 생명과 환경, 지구와 순환의 3가지 내용 영역의 소재를 중심으로 총 30문항을 개발하였다. 개발된 문항을 과학교육 전문가에게 2회 의뢰하여 타당도, 객관도, 문항의 명료성을 점검 받았으며 2번의 현장 검증을 통해 신뢰도, 변별도, 난이도를 검증하였다. 그 결과 타당도 91.6%, 신뢰도 지수 Cronbach α값 0.79, 객관도 93.3%, 변별도 지수 0.30, 난이도 지수 66.1%로 나타났다. 위와 같은 값들은 평가도구가 갖추어야 할 구비조건의 허용 범위내에 있는 것으로 보아 본 연구에서 개발한 과학탐구능력 평가도구는 양호한 것으로 볼 수 있다. 따라서 본 연구에서 개발한 과학탐구능력 평가도구는 초등학생들의 과학탐구능력 성취 수준의 진단, 과학과 교육과정과 교수?학습 자료 및 과학과 교수ㆍ학습 방법의 과학탐구능력 반영 수준을 평가하는 도구로도 이용될 수도 있을 것이다. The purpose of this study is to develop a valid and reliable evaluating instrument for elementary school fifth and sixth graders. The instrument is developed through R&D procedure, which includes two checks of science specialist and two field trials of the instrument. Evaluating items are content-free for each science inquiry skill. Each science inquiry element is based on SAPA and the 7th curriculum. This study has selected 10 science inquiry skills(observing, classifying, measuring, predicting, inferring, recognizing of a problem, controlling variables, interpreting data, drawing a conclusion, designing an experiment), formulated a clear definition of the elements of science inquiry skills, and established the objectives of evaluation. The content areas are divided into three categories, material and energy, life and environment, and the earth and circulation. Each category contains 10 items. So the instrument consists of 30 items. The content validity of items, objectivity of the scoring keys, and clarity of the items has been checked twice by specialists in science education. At the same time, two field trials were performed to produce the reliability of the instruments, discrimination index, and item difficulty index. The instrument has the content validity is 91.6%, reliability 0.79, objectivity 93.3%, discrimination index 0.30, and item difficulty index 66.1%.

건축공사시방서에서의 KS규격의 국제인증방안에 관한 연구

김경식,이재섭,김용수,이규진,조용훈,최완철,전재열,이현수,구재동,이기환 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The purpose of this research is make out construction specifications for International Construction. Using off-shore products incur cost increase and longer delivery term of construction. and inconvenient on International Construction. Expand the range of additional local products conforming to performance. Life Cycle Cost. maintainability. operability. user's acceptability, etc.. And this study is suggest the standard formats and the techniques for construction specification.

肝吸蟲感染에 따른 血淸 IgE와 血液內好酸球의 變動樣相에 관한 硏究

朱炅煥,林漢鍾 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.1

The present study aimed to clarify the influence of single or repeated infection of Clonorchis sinensis on the IgE level and eosinophil counts of rabbit, and to determine the relationship between degree of infection and eosinophil counts or IgE level in the patients infected with C. sinensis. Various numbers of metacercariae isolated from flesh of the second intermediate host, Pseudorasbora parva, were directly introduced to the rabbits. Single infection group was divided into heavy, moderate and light infection groups, in which 2,000, 500 and 50 metacercariae of C. sinensis were administered respectively. Repeated infection group was also divided into heavy, moderate and light infection groups, in these cases 200, 100 and 10 metacercariae were fed biweekly for five times respectively. Infection was confirmed by detection of the eggs of C. sinensis in feces five weeks after primary infection. Whole blood and sera were collected prior to infection, and on 1,3,5,7, and 9 weeks after infection from the inner marginal vein of rabbit's ear respectively. As a control, whole blood and sera were also sampled from the non-infected rabbits. Total eosinophil counts, differential count, and IgE levels were examined. On the other hand, a total of 50 patients infected with C. sinensis was examined their degree of infection (EPG), total eosinophil counts and differential counts of white blood cells of 50 c. sinensis infected patients were exanined. Forty-six sera were sampled from patients infected with C. sinensis and the serum IgE levels were evaluated. In analysis of serum IgE level, Phadebas IgE PRIST® radioimmunoassay technique was applied. The results are as follows: 1. Total eosinophil counts of repeated infection group of rabbits were significantly higher than that of single infection group. Total eosinophil counts of non-infected rabbits showed ranges of 0~65/㎣ blood during 9 weeks of examination. In the infected rabbits of heavy infection group, total eosinophil counts increased rapidly and reaching to peak on the 5th week after infection. These values maintained until the end of examination in 9th week (140/㎣ in single and 250/㎣ in repeated group). However, total eosinophil counts of light and modertate infection did not show rapid increase. On the contrary they increased slightly and continuously until 9 weeks after infection. Similar results were obtained from the differential counts of eosinophil. 2. In the examination of the patients infected with C. sinensis correlation between EPG and eosinophil count was recongnized. Coefficient of correlation was, r=0.61, rank correlation r=0.700, and regression equation was Yc(total eosinophil counts)=0.04χ (EPG)+372. 3. Total serum IgE levels in the patients infected with C. sinensis showed remarkably elevated value. mean values were 1,167 IU/㎖±1,172, but correlation with degree of infection was not significant statistically. Influences of repeated infection of C. sinensis on the IgE level were not able to investigate, because IgE was not detected in Phadebas IgE PRIST® using rabbit as an experimental animal.

첨가된 질량이 있는 단순지지된 샌드위치 패널에 대한 간편한 진동해석의 적용

김성환,이정호,정경일,이봉학 江原大學校 産業技術硏究所 1996 産業技術硏究 Vol.16 No.-

Many of the bridge systems, including the girders and cross-beams,and concrete decks behave as the special othotropic plates. A method of calculating the natural frequency corresponding to the first mode fo vibration of beam and tower structures with irregular cross-sections was developed and reported by D.H.Kim in 1974. Since 1989, The author has extended this method to Vibration analysis of two dimensional problems includign composite laminates, and has reported at several conferenes. Frequently, the bridge floor panels are supported by girders and cross beams. Such panels as well as some of the building floor panels can be assumed as simple supported special orthotropic plates. In this paper, the result of application of simple method fo vibration analysis developed by D.H.Kim, to the simply supported sandwich panels woth point Mass/Masses is presented.

생분해성 다공질 Calcium Phosphate 세라믹스의 제조

이경주,이중환,김석영 嶺南大學校 工業技術硏究所 1995 工業技術硏究所論文集 Vol.23 No.2

When porous calcium phosphate ceramics are used as a bone graft substitute, it is known that new tissue or blood vessel is grown into the porous implant due to their excellent biocompatibility. In this study, the most chemically stable β-crystalline form of calcium metaphosphate, Ca(PO₃)₂is prepared by the controlled thermolysis of monocalcium phosphate, Ca(H₂PO₄)₂. The diameter of cylindrical pores formed during cooling was controlled by a holding time at the melting point of a monocalcium phosphate and by the change of a recrystallization temperature, to obtain the most appropriate size(about 200㎛) of pores. It was observed that the increasing holding time at the melting point of monocalcium phosphate results in the decreases of pore sizes.

덕트 내부 소음의 능동 소음 제어

최경호,김일환 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-

The purpose of this present experiments was to simulate the Active noise control system using MATLAB Tool kit. The Least-Mean-Square algorithm is the most applicable one to optimize the ANC systems, even it has tight limitation. This paper shows the influence of choosing step size to the performance of the LMS adaptive filters. In addition to the simulation, this paper describes the method to design the filtered LMS algorithm to get the better performance in Active noise control. It contains the secondary-path modeling to realize the real Active noise control system in the requesting field