p-Anisaldehyde가 조골세포 분화에 미치는 영향

김경민 ․ 이도원 ․ 김현준 ․ 김아랑 ․ 장원구 대구대학교 산업기술연구소 2018 産業技術硏究 Vol.29 No.2

p-Anisaldehyde is a natural fragrance extracted from Pimpinella anisum L., and used as a preservative. This study examined the effect of p-anisaldehyde on osteoblast differentiation. First, cytotoxicity tests were carried out after examining the concentrations that did not show toxicity to the cells. The expression of osteoblast differentiation marker gene was confirmed by RT-PCR and was not effective in the treatment with p-anisaldehyde alone. However, p-anisaldehyde decreased the expression levels of inhibitor of differentiation-1 (Id1), distalless related homeobox (Dlx5), and runt-related transcription factor 2 (Runx2), which are osteogenic differentiation marker genes, which are increased in osteogenic condition. alkaline phosphatase (ALP) activity was also confirmed by ALP staining that p-anisaldehyde reduced ALP activity. These results show that p-anisaldehyde is effective in reducing osteoblast differentiation.

벼 未熟種子의 休眠과 發芽抑制物質에 對하여

崔京求,李王休 全北大學校 1980 論文集 Vol.22 No.-

The present study was carried out to clarify the inducing factors of seed dormancy of 12 newly improved rice varieties by checking the effect of hull and germination inhibitors on seed germination of 30 day-old rice seeds. The obtained results were as follows; 1. Hulled or half-hulled rice seeds showed remarkably high percentage of germination and rapid germination speed. Therefore, the dormancy of immature rice seed seemed to be mainly due to its hull. 2. Soaking seeds in water at 30℃ had an effect on breaking dormancy and the effect tended to be decreased in inverse proportion to the degree of seed dormancy. 3. Through bioassay with lettuce and rice seed germination, the existence of germination inhibitors which leached into water from rice seeds was recognized. In general, high dormant rice varieties tended to have less water soluble inhibitors than low ones. 4. The inhibitors in rice seeds were detected in the zones of Rf 0.1-0.3 and Rf 0.8-1.0 even though the patterns of paper-chromatogram on inhibitors in seeds differed with varieties. 5. The amount of germination inhibitors in seeds was closely but not completely related with the degree of seed dormancy. Therefore, it may be reasonable to evaluate the degree of immature rice seed dormancy with the amount of inhibitors in the seed and that of leaching inhibitors from it. 6. In some varieties such as Iri 332, Milyang 23, and Milyand 29, the seed dormancy was also due to the physiological immature of embryo as well as the effect of hull and inhibitors. 7. Considering the above conditions, the tested 12 varieties may be grouped as follows; a. Low dormant varieties: Tongil, Josaengtongil, Suweon 264, Tongilchal, Milyang 21. b. Medium dormant varieties: Iri 326, Iri 327. c. High dormant varieties: Iri 332, Yushin, Milyang 23, Milyang 29, Milyang 30.

人蔘의 育種 및 栽培年限 短縮에 關한 基礎硏究 : 第1報 新芽의 體眠打破에 미치는 植物生長調節物質의 影響 Effect of some growth regulators on the dormancy breaking of new buds

崔京求,金鎭淇,黃種奎 全北大學校 1978 論文集 Vol.20 No.-

This study was carried out to investigate the effect of some growth regulators i.e., gibberellic acid (GA), benzyladenine (BA) and abscisic acid (ABA) on the dormancy breaking of new buds in 2 year-old Ginseng plants. Materials were sampled in the middle of October and their dormancy was checked under the favorable conditions for germination of bud for a month. They were treated for 24 hours with the following growth regulators; (1) GA, BA and ABA at the concentrations of 50 and 100 ppm, and (2) the combination of GA and BA, GA and ABA, and BA and ABA at the concentrations of 50 ppm, respectively. It was found that the application of GA was effective in dormancy breaking of new buds and seemed to elongate stem, flower stalk and petiole but degenerated the majority of flower stalk in the early stage and also accelerated the formation of the adventitious root. By the application of GA, poor development of color was observed in leaf and stem in the early stage. The leaf began to regain green color with leaf maturation but the stem did not gain purple color. The applications of BA and ABA combined with GA showed almost the same effect in dormacy breaking as GA application. In these cases color development of stem and leaf was good cmpared with that of GA application. Even with some side-effects such as elongation and poor color development of stem and leaf, GA might be applied for dormancy breaking of new buds substituting for the low temperature in winter.

동진강 정읍천 유역 오염부하량 평가

이경보,김종천,박지혜,이덕배,김종구 한국환경농학회 2004 한국환경농학회지 Vol.23 No.1

하천 수질관리 대안을 제시하고자 동진강에 영향을 가장 크게 미치는 정읍천을 대상으로 2002년 1월부터 2003년 12월까지 점 및 비점 오염원으로부터 수질오염물질의 부하량, 유출 경로 등을 평가하였다. 계절별 수질 변화는 pH 범위가 6.87∼7.53으로 봄의 pH 범위가 컸으 며, EC 농도는 정읍천 중류가 높았으며, 상류유역은 83∼95㎲/㎝ 범위를 나타냈고, 계절별로 가을에 517㎲/㎝로 가장 높았다. BOD의 농도는 상류유역에서 0.61∼1.27 ㎎/L로 Ⅰ급수 수 질은 나타내었으나, 중류와 하류에서는 Ⅲ등급 수준이었다. T-N의 농도는 정읍천 중류 유 역이 6.10∼10.84㎎/L로 가장 높았고, T-P의 농도는 타오염물질에 비해 농도의 편차가 컸으 며, 특히 중류에서 T-P의 농도가 0.41∼0.98 ㎎/L로 높았다. BOD의 배출부하량은 J4 유역에서 553 ㎎/day로 가장 많았고 T-N 발생부화량은 가축, 인 구, 토지이용, 산업의 순으로 컸으며, T-N 배출부하량은 인구 및 산업에 의한 것은 J4 유역, 가축과 토지이용에 의한 부하량은 J5, J6유역에서 높았다. T-N의 유달부하량은 하류 유역에 서 높았으며, T-P의 유달 부하량은 BOD, T-N에 비하여 유달부하량이 낮았고 유달율은 6∼38% 범위를 나타냈다. The influence of pollutant loads on the water quality in Jeongeupcheon of Dongjin river was evaluated from Jan. 2002 to Dec. 2003 for two years. The range of pH in water was 6.87-753. The EC level in upstream ranged from 83 to 95 us/cm with the highest value in autumn. The BOD level in upstream ranged from 0.61 to 1.27 ㎎/L, which would be Ⅰ grade according to water quality criteia by Ministry of Environment, but that in downstream was Ⅲ grade. The average T-N level in midstream range from 6.10 to 10.84㎎/L which was the highest values throughout the stretch of the river. The average T-P levels ranged from 0.41mg to 0.98㎎/L. Jeongeupcheon was suitable for the agricultural usage based upon one year analysis of river water quality. The effluent loads of BOD was high in midstream(J4) with 553 ㎏/day. The major sources of T-N loads were livestock, population, land use, and industry in order. The effluent loads of T-N was high in J4 by population and industry while that of T-N was high in J5 and J6 by livestock and land use. The delivered loads of T-N was high in downstream. The delivered loads of T-P was low as compared with those of BOD and T-N. The delivery ration of T-N ranged from 6 to 38%.

만경강 본류의 자연정화능 향상을 위한 식생학적 진단

이경보,김창환,김종구,이덕배,박찬원,나승용 한국환경농학회 2003 한국환경농학회지 Vol.22 No.2

만경강 본류를 대상으로 수질을 개선하기 위한 일환으로 하천식생의 종구성, 하천형태에 따른 군락의 구조, 단면분석을 통한 현존식생의 분포 등을 종합적으로 분석하여 하천 생태계에 대한 종합적 해석과 식생에 의한 자연정화능 활용방안을 평가하고자 2001년 6월부터 2002년 3월까지 수질 및 식생에 관한 자연정화능을 조사, 평가하였다. 만경강 본류 수질중 T-N의 농도는 갈수기인 겨울철이 17.23 mg/L로 가장 높았으며, 여름철이 8.59 mg/L로 낮았고, 상류보다는 하류에서 그 농도가 높았다. 또한 평균 T-P의 농도는 여름철과 가을철에 각각 0.45, 0.47 mg/L로 비슷하였으나 겨울철에 1.79 mg/L로 농도가 높아졌다. BOD의 농도는 상류유역에서 0.95~2.57 mg/L로 Ⅰ~Ⅱ급수 수질을 나타내었으나 하류유역에서는 6.87~9.72 mg/L로 Ⅴ급수에 가까운 수질이었다. 만경강 상류의 식물상은 73과 134속 218종 33변종으로 총 251종류가 조사되었으며, 중류 일대에서 조사된 식물은 33과 69속 82종 16변종으로 총 98종류가 조사되었다. 그리고 만경강 하류 일대에서 관찰된 식물은 27과 58속 71종 14변종으로 총 85종류가 조사되었다. 만경강 유역에서 자연정화능에 의한 수질개선을 도모하기 위해 식물 생활형별로 하천 정화능이 뛰어난 식물을 조사 분석한 결과 침수식물은 붕어마름, 검정말, 부엽식물은 어리연꽃, 왜개연꽃, 추수식물은 갈대, 줄, 부들, 습생식물은 큰황새 냉이, 고마리 등이 질소, 인 흡수능이 우수한 식물이었다. This study was conducted to get some information on plants abilities to enhance water purification and to find out away to conserve the ecosystem in Mankyeong river. Vegetation were surveyed at 4 sites pointing by 1:5,000 topographical map, from June 2001 through March 2002. T-N content in water were high in all sites of Mankyeong river, the average T-N levels were 8.59 and 17.23 ㎎/L summer and winter, respectively. The average T-P level during summer was 0.47 ㎎/L, but that was 1.79 ㎎/L during winter. The BOD level in Mankyeong upstream ranged from 0.95 to 2.57 ㎎/L which would be in Ⅰ or Ⅱ grade according to water quality criteria by Ministry of Environment, but BOD level in Mankyeong downstream ranged from 6.87 to 9.72 ㎎/L. The plant species of river flora were found 251, 98 and 85, upstream, midstream and down stream, respectively. Among the surveyed plants, Ceratophyllum demersum, submerged plant and Nuphar subinteperrimum took up higher contents of phosphate and nitrogen than other plants. The Phragmites communis and Zizania latifolia having much biomass were thought to be suitable plants for enhancement of the natural water purification.

만경강 상류 수질 및 식생분포와 토양환경에 따른 하천식생의 종 다양성

이경보,김창환,이덕배,김종구,박찬원,나승용 한국환경농학회 2003 한국환경농학회지 Vol.22 No.2

만경강 상류유역 수질 및 하천식생 군락 변화를 조사 분석하여 수질에 따른 하천식생의 종 다양성 및 토양요인에 따른 종 다양성의 변화를 평가하기 위하여 2001년 6월부터 2002년 9월까지 만경강 상류유역 5개 조사지점을 선정한 후 조사를 실시하였다. 수질중 T-N의 농도는 전주천과 삼천에서 높았으며, 겨울철에 전주천에서 7.45 mg/L로 가장 높았다. 무기태 질소 성분벽 함량은 NH₄-N 경우 고산천과 소양천 유역은 0.01~0.06 mg/L 범위로 계절별 커다란 차이가 없는 낮은 농도를 나타냈으나, 생활하수가 유입되는 전주천과 삼천에서는 0.77~3.01 mg/L로 계절별로 큰 차이를 보였다. T-P의 농도는 계절별로 비슷하였으나 전주천에서 겨울천에 0.89 mg/L로 가장 높았다. 계절별 BOD의 농도는 고산천과 소양천 유역에서 0.92~2.14 mg/L를 나타내었으나 생활하수가 유입되는 유역에서는 2.01~7.65 mg/L범위를 보였다. SO₄²^(-)의 농도는 농업용수 수질기준인 50 mg/L를 초과하는 지역은 없었으며 소양천에서 다소 높았다. 만경강 지류인 고산천에서 조사된 식물은 59과 과 129속 165종 20변종으로 총 185종류가 조사되었으며, 소양천에서 조사된 식물은 53과 111속 141종 19변종으로 총 160종이었고, 전주천 하류에서 조사된 식물은 37과 68속 86종 15변종으로 총 103종이었다. 그리고 삼천에서 조사된 식물은 32과 92속 110종 18변종으로 총 128종류 조사되었으며, 만경강 본류인 하리유역에서 조사된 식물은 73과 134속 218종 33변종으로 총 251종류가 조사되었는데 식물의 생활형에 따라 분류하면 침수식물이 13종, 부엽식물이 5종, 부유식물이 2종, 추수식물이 26종, 수생식물이 46종, 습생식물이 47종으로 조사지점중 이 지역의 식생이 가장 다양하였다. 식물사회학적 방법에 따라 분류된 하천식생의 식물군락은 10개의 수생식물 군락과 2개의 습생 및 수변식물 군락으로 대별되어졌다. 이들 군락들에 대한 4종류(종수, 종의 풍부도지수, 종의 이질성지수, 종의 균등도지수)의 종 다양성과 토양요인(pH, EC, 유기물 함량, 전질소, 인삭)과의 관계를 분석한 결과 pH가 높을수록 4종류의 종 다양성 지수는 높아 졌으며, EC는 값이 높을수록 종수(SN), 종의 풍부도지수(SR), 종의 이질성지수(H'), 종의 균등도지수(J')가 낮아 졌다. 또한 유기물 함량(OM)과 전질소(T-N)의 경우도 4종류의 다양성지수와는 부의 상관관계를 나타내었다. 인산(P₂O_(5))은 4종류의 다양성 지수의 변화에 영향을 미치지 않았다. This study was conducted to evaluate influence of chemical properties in the riparian on the species diversity and to get plant information for enhancement of natural purification in Mankyeong River. The concentration of total nitrogen was high in Jeonju and Sam stream, while that of total nitrogen showed the highest peak in Winter. Concentrations of NH₄-N was 0.01-0.06 ㎎/L in Gosan and Soyang stream. The water quality of upstream along with Mankyeong River was suitable for the imgation source. The riparian vegetation was investigated by Zurich-Montpellier school's method from June, 2001 to September, 2002. The number of riparian plants were 59 families, 129 genera, 165 species, 20 varieties in Gosancheon, on the while 53 families, 111 genera, 141 species, 19 varieties in Soyangcheon The number of riparian plants in Hari basin was higher than that of other sites namely, 73 families, 134 genera, 218 species, 33 varieties. Riparian vegetation was consisted of 12 plant communities. The contents of organic matter, total nitrogen and electrical conductivity had negative relationship with species diversity (Species richness index, Heterogeneity index, Species evenness index, Species number). On the while, species diversity had positive relationship with soil pH. Species diversity of the plant communities were affected by topography and disturbance.

동진강의 식물상과 식생

이경보,김창환,이덕배,김종구,박찬원 한국환경농학회 2004 한국환경농학회지 Vol.23 No.1

동진강 일대의 인위적인 교란에 의한 하천식생의 복원 및 복구를 위한 기초적인 자료를 제공하고자 2001년 6월부터 2002년 3월까지 하천식생의 종 구성, 하천형태에 따른 군락의 구조, 현존식생의 분포 등을 종합적으로 분석한 결과는 다음과 같다.동진강 유역에서 조사된 하천식생은 73과 188속 238종 33변종 1품종으로 총 272종류가 조사되었다. 또한 관속식물의 생활형에 따라 분류하면 침수식물이 23종, 부엽식물이 7종, 부유식물이 2종, 정수식물이 18종, 습생식물이 53종, 수변식물이 168종이었으며, 수생식물과 습생식물은 각각 50종과 53종류로 전체 조사된 272종류 중 37.9%를 차지하였다. 식물생활형의 분포비율은 휴면형 1년생 식물이 97종 35.7%, 수생식물이 78종 28.7%, 반지중식물이 41종 15.1%, 지중식물이 22종 8.1%, 지표식물이 12종 4.4%가 관찰되었다.동진강 상류유역의 식물군락은 검정말군락, 검정말-실말군락, 붕어마름군락, 달뿌리풀군락, 고마리군락, 큰황새냉이군락, 여뀌군락 등 총 8개의 식물군락이 분포하였다. 동진강 중류유역은 수생식물인 줄과 갈대군락, 그리고 수변식물군락으로 고마리군락, 교란지식생인 환삼덩굴군락 등 총4개 군락이 분포하였고, 동진강 하류 유역은 자라풀군락, 붕어마름군락, 마름군락, 줄군락, 갈대군락의 5개 습생식물군락과 털물참새피군락, 고마리군락의 2개 습생식물군락, 새콩 등의 나대지 식물군락 등 총 8개 식물군락이 관찰되었다 This study was conducted to get some vegetation information and to find out a way to conserve the ecosystem in the Dongjin River. The riparian vegetation was investigted by Zurich-Montpellier school's method from June 2001 to March 2002. The number of riparian plants were 73 families, 188 genera, 238 species, 33 varietles or 272 Taxa in Dongjin River. The characteristics of life form spectra were 97 therophytes(35.7%), 78 hydrophytes (28.7%), 41 hemicryptophytes (15.1%) 22 geophytes (8.1%), and 12 chamaephytes (4.4%). The riparian vegetaton was identified 8 plant communities (Potamogeton brechtoldii, Hydrill verticillata, Ceratophyllum demersum, Potamogeton malaianus, Phragnites japonica, Persicaria thunbergii, Cardamine scutata, Persicaria hydropiper) in upstream, 4 plant communities(Zizania latifoliar, Phragmites communis, Persicaria thunbergii, Hunmulus japonicus) in midstream and 8 plant communities(Hydrocharis dubia, Ceratophyllum demersum, Trapa japonica, Zizania Iatifolia, Paspalum distichum, Phragmites communis, Persicaria thunbergii, Amphicarpaea edgeworthii) in downstream of the Dongjin River.

人蔘種子에 있어서 後熟過程中 內生 Hormone의 變化

崔京求,張熙振,柳南熙 全北大學校 1994 論文集 Vol.37 No.-

This study was conducted to investigate the seasonal changes of inhibitors during after-ripening of the ginseng seeds. The bioassays of lettuce seed germination rate to the water and chlorogorm soluble fraction extracted from ginseng seeds during stratification were applied. The obtained results were summarized as follows: 1. Three kinds of germination inhibitors were detected in the water soluble fraction of the after-ripening seeds, and the activity of inhibitors was more increased at the Rf 0.1 zone than that of the Rf 0.4-0.5 and 0.8-1.0 zone. 2. Chroloform soluble fraction showed the similar tendency to the water soluble fraction, and promoter was recognized at the Rf 0.1-0.2 zone in the seeds of 90th day after stratification. The inhibitor of the Rf 0.1-0.2 zone increased in accordance with stratification, and that of the Rf 0.9-1.0 zone decreased slightly. 3. On the whole, the physiological activities of three kinds of germination inhibitors from the water soluble fraction showed more increase than those of the chloroform soluble fraction. 4. The water and chloroform soluble 'faction from the embryos of ginseng seeds during stratification showed two kinds of germination ingibitors at the Rf 0.4-0.5 and 0.9-1.0 zones, and water soluble fraction from the endosperms of ginseng seeds showed three kinds of inhibitors at the Rf 0.1-0.2. 0.4-0.5 and 0.7-1.0 zones.

옥수수 播種期와 地上部形質에 관한 硏究

金鎭淇,崔京求,白南赫,陳星桂 全北大學校 1982 論文集 Vol.24 No.-

全北 平野地인 裡里地方에서 옥수수의 播種期와 地上部生育 및 種實收量과의 관계를 調査하여 다음과 같은 결과를 얻었다. 1. 播種期의 早晩이 莖葉의 生育 및 種實收量에 크게 영향을 끼치며 播種이 늦어질수록 出雄, 出絲, 生育日數가 짧아지고 地上部生育은 떨어지는 경향이다. 2. 莖葉生育은 4月 20日 播種을 基點으로, 種實收量은 5月 5日 播種을 基點으로 各各 減少되었다. 3. 地上部諸形質과 種實收量간에는 高度의 正의 相關이 인정되며 種實增收를 위하여는 莖葉生育은 크게 할 필요가 있다. Some results were gained through the experiment that was carried out with a single cross cultivar, Suweon 19, of maize (Zea mays L.) in Iri, the Jeonbug plain area, in 1980, to check relationships between growth characters at different sowing dates. 1. The foliage growth and grain yield were greatly influenced with planting season. The number of days to tasseling, silking, or maturing shortened and the growth of maize decreased as sowing was delayed. 2. The threshold sowing date for foliage production seemed to be April 20 and that for grain yield, May 5. 3. Highly significant positive correlation existed between vegetative growth and grain yield. This may mean that increasing vegetative growth is prerequisite to better grain yield.

新品種 밀 "早光"의 沓裏作 播種期 및 播種量에 관한 硏究

黃鍾奎,崔京求,陳星桂 全北大學校 1979 論文集 Vol.21 No.-

This experiment was carried out under different seeding dates(October 10, Oct. 20 or November 10) and seeding rates (20, 30, 35 ℓ 10a) from 1976 to 1977 in Iri in order to study the suitable seeding date and seeding rate for Jokwang, the newly developed wheat variety, in double cropping paddy field. 1. Number of days to seedling emergence both on Oct. 20 and Oct. 30 were 14 days, but that of Nov. 10 was prolonged due to comparatively low temperature and about 37 days. 2. The heading date and the maturing date seemed to be shortened by 1 or 2 days in accordance with the earlier seeding date and the larger amount of seeding. 3. Number of panicles per square meter seeded on Oct. 20 was 506 which marked the most among the three seeding dates. 30ℓ 10a plot showed the most in panicle number among three seeding rates. 4. Number of grains per panicles was increased by 1 or 2 grains with hastened seeding date, but it was decreased by 2 or 3 grains with the increase in seeding rate. 5. The weight of 1,000 grains of the plots both seeded on Oct. 20 and Oct. 30 was about 38.0 g. and that of Nov. 10 was 35.0g. On the other hand, there were almost negligible differences in 1,000 grains weight between seeding rates at the same seeding date. 6. The yield of the plot seeded on Oct. 20 was 359 ㎏ 10a which marked the highest among the three seeding dates. Among seeding rates, 30 ℓ/10a plot showed the highest yield of 351 ㎏ 10a. However, the highest yield among all treatments, seeding dates and seeding rates, was 382 ㎏ 10a which was obtained from the plot seeded on Oct. 20 with the seeding rate of 30 ℓ/10a. 7. Therefore, it was considered that the suitable seeding date for Jokwang, the newly developed wheat variety, in double cropping paddy field in Iri area was Oct. 20 and its seeding rate was 30ℓ/10a.