토양에서 분리된 Acrylamide 분해 세균 JK-7의 분리 및 특성

천재우,오계현 순천향대학교 기초과학연구소 2003 순천향자연과학연구 논문집 Vol.9 No.2

The feasibility of using pure culture for acrylamide degradation, with the ultimate aim of application for biological treatment process, was explored. The present study reports on the test cultures which were developed to grow aerobically with acrylamide as the sole source carbon and nitrogen. A bacterial isolate, strain JK-7 was isolated from paddy soil samples, Strain JK-7 could degrade 50 mM acrylamide completely within 72 hours of incubation. Major intermediates resulting from acrylamide degradation were not detected with the HPLC methodology except acrylic acid which appeared to accumulate transiently in the growth medium. Initial pH 7.2 of the cultures became to increase 8.4 at the end of incubation. When JK-7 cells were grown at over 100 mM acrylamide, there was a pause of cell growth, resulting in a reduction in the rates of acrylamide degradation. Survival test revealed that cells exposed to low concentrations of acrylamide enable to the strain JK-7 to survive at a lethal concentration of 100 mM acrylamide. The relationships between the acrylamide degradation by JK-7 and several relevant physicochemical environmental parameters were examained. The effect of supplemented carbons(e.g., glucose, fructose, citrate, succinate) on the acrylamide degradation by the test culture of JK-7 was evaluared. The results indicated that the addition of carbons accelerated the bacterial growth and acrylamide degradation compared to in the absence of supplemented carbons. The effect of supplemented nitrogens on the degradation was monitored. Increasing concentrations of yeast extract resulted in higher growth yield, based on the turbidity measurement, and complete degradation of acrylamide. However, acrylamide degradation was essentially uninfluenced by the addition of (NH_(4))_(2)So_(4),NH_(4)Cl, or urea. The bacterium was identified as belonging to the genus Pseudomonas in the basis of use BIOLOG test, and designated as Pseudomonas sp. JK-7

한 개의 지중 전열선에 의한 열전달 분포에 관한 연구

전계웅,윤영환 國立 昌原大學校 産業技術硏究所 1999 産技硏論文集 Vol.13 No.-

Heat transfer in soil has been interested recently in fields of air conditioner, heat loss from buried pipes and especially floor heating in agricultural greenhouse for better production of the crops. In this study, temperature distributions from an electrically heated pipe buried under ground have been measured by thermocouples and analyzed by numerical solution of two-dimensional heat conduction equation with a commercial program, NISAⅡ. From the study, the temperature distributions in the soil were relatively sensitive to the thermal diffusivity (α) but almost invariable to surface heat transfer coefficient(h). When thermal diffusivity, α is equal to 0.543×10??㎡/s, there are good agreements between experimental data and numerical solutions. The value of thermal diffusivity is almost upper limit of those from several references according to wetness of soil. Therefore, it can be deduced the condition of soil was fully wetted.

해양에서 분리한 Lantobacillus sp. JK-8에 의한 질병원인 세균의 살균효과

천재우,마채우,오계헌 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The purpose of this work was to investigate for killing effect of disease-causing bacteria by Lactobacillus sp. JK-8 isolated from marine environment. Initially, a bacterial culture, strain JK-8 was developed to grow no MRS media. The bacterium was identified as genus Lactobacillus on the basis of BIOLOG test, and designated as Lactobacillus sp. JK-8. The intial pH 7 of the cultures became to decrease pH 3.85 at the end of incubaction according to the growth of Lactobacillus sp. JK-8. The antibacterial activity using plate diffusion method against target bacteria was determined with 5-fold concentrate of cell-free culture supernatant. Excellent killing effect of target bacteria was achieved. The inhibition zone obtained with culture supernatant were in between 14 mm and 20 mm. Killing rate of pathogenic bacteria was examined with cell-free concentrated supernatants. All bacteria tested in this work completely killed within 3 hrs of incubaction.

內科的 疾患에 있어서 스포츠의 疾病管理

田桂植 龍仁大學校 1990 용인대학교 논문집 Vol.6 No.-

The management of disease for sports is the most important component for modern people who are prone to cause the physical inadaptability, and psychological disability and as well, raises the physical enhancement, general vitality, an optimal level of performance, appearance and better health. The management of diseases for sports has a wide range of fields and is discussed only from the viewpoint of the internal medicine through literature. Prolonged, rhythmical and adequate exercise has a modest beneficial effects on the blood pressure, coronary atheroscleroses, tuberculosis, heart diseases, protein urine and so on. Participation in such regular isotonic exercises as walking, cycling, swimming, jogging may be acceptable and an additional incentive to keep up the acquired athletic habits. The mode, frequency, duration and intensity in exercise prescription should be selected with great care because the patient may have complications, and when properly performed, the patient should continue exercise program for 30 to 60 minutes per day by several studies, at least three to four days per week. The maximal oxygen uptake of the substantial training effect can be accomplished by training at between 50 and 80 percent of your aerobic capacity. All preconditions for efficient training are to be taken into considerations. The patient with Compensated valvular diseases, obesity, hypertension, diabetes. myocardial infarction, angina proctor is should perform proper exercises and training according to his individual situation. Contraindications include manifest heart failure, recent infarction, severe tuberculosis, aortic aneurysma, severe angina pectoris, arrhythmias with ventricular premature beats including with exercise.

분사식 주사기를 이용한 소아환자의 국소마취

이재춘,김대업,이광희,김성형,양계식 大韓小兒齒科學會 2001 大韓小兒齒科學會誌 Vol.28 No.4

소아치과에서 국소마취는 공포의 대상이며, 특히 행동조절이 힘든 소아의 경우 더욱 그러하다. 국소마취에 대한 공포는 주사침의 자입시나 주사액의 주입시 느끼는 동통 만큼이나 주사침 자체의 공포도 크게 차지한다. 주사침 자입시의 동통을 완화하기 위하여, 도포마취제 등이 개발되었으나 이들은 국소마취의 동통을 완화시키는 면은 있으나 주사침 자체에 대한 공포를 없애지는 못하였다. Syrijet Mark Ⅱ??(Mizzy Inc, USA)는 주사침이 없는 치과용 분사식 주사기(jet injection device)로서 소아에서 주사침에 대한 공포를 성공적으로 감소시킬 수 있다고 알려져 있다. 저자들은 여러 행동등급의 소아를 대상으로 Syrijet을 사용하여 국소마취를 시행한 후 동통평가의 척도로서 VAS(visual analogue scale)를 소아에게 적합하게 색생화한 CAS(color analogue scale)을 이용하여 국소마취시의 동통수준을 측정한 결과, 기존 주사침에 의한 국소마취시 보다 국소마취의 동통이 감소된 것을 알 수 있었다. Syrijet의 장점으로는 주사침이 없다는 점, 기존의 술식에서 일회용이었던 미취용 카트리지를 재사용할 수 있다는 점, 신경 및 혈관에 안전하다는 점 등이 있었으며, 단점으로 부피가 크며, 가격이 비싸며, 사용시 숙련성을 요구한다는 점 등이 있었다. Local anesthesia procedure in pediatric dentisty using needle-tipped syringes is stressful and painful for the child patients. The Syrijet Mark Ⅱ (Mizzy Inc. USA) is a jet injection instrument, which uses high pressure to propel fluids into soft tissue without the use of a hollow neeldle, so it seems to be able to reduce the injection phobia of the child patients. The authors compared the Syrijet with the conventional syringe by assessing the pain level after local anesthetic procedures using CAS(color analogue scale) which was developed from VAS(visual analogue scale). The result showed that the pain was reduced by the use of syrijet. The advantages of syrijet were no use of neeldes, the reuse of the anesthetic cartridge, and the safety to nerves and vessels. The disadvantages were the large size, the high price, and the need of skill to use.

녹차추출물에 의한 식중독 원인균인 장내세균 Plesiomonas shigelloides의 세포반응

유미옥,천재우,오계헌 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.2

The aim of this work was to investigate the cellular responses in the food-poisoning bacterium, Plesimonas shigelloids in response to tea polyphenol (TPP) extracted from green tea. The stress shock proteins, which contribute to the resistance of the cytotoxic effect of TPP, were induced at different TPP concentrations in exponentially growing culture of P. shigelloides. This response involved the induction of a 70-kDa DnaK and a 60-kDa GroEL monoclonal antibodies, characterized by SDS-PAGE and Western blot by the use of the anti-DnaK and anti-GroEL monoclonal antibodies. Survival of P. shigelloides with time in the presence of different concentrations of TPP was monitored, and viable counts paralleled the induction of the stress shock proteins in this strain. This strain killed in the concentration of 1,000 ㎍/㎖ TPP within 2 hours of incubation. Scanning electron microscopy for the cells treated with 10,000 ㎍/㎖ for 12 hours showed the presence of perforations and irregular rod shaped with wrinkled surfaces.

Tea polyphenol에 노출된 식중독 원인세균 Listeria monocytogenes의 살균

유미옥,천재우,오계헌 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The aim of this work was to investigate the killing effect of the food-poisoning bacterium, Listeria monocytogenes exposed by tea polyphenol (TPP) extracted from green tea. The stress shock proteins, which contribute to the resistance of the cytotoxic effect of TPP, were induced at different TPP concentration in exponentially growing cultures of L. monocygenes. This response involved the induction of a 70-kDa DnaK and a 60-kDa GroEL monoclonal antibodies, characterized by SDS-PAGE and Western blot by the use of the anti-DanK and anti-GroEL monoclonal antibodies. Survival of L.monocytogenes with time in the presence of different concentrations of TPP was monitored, and viable counts paralleled the induction of the stress shock proteins in this strain. This strain killed in the concentration of 1,000㎍/㎖ TPP within 4 hours of incubation. Scanning electron microscopy for the cells treated with 1,000㎍/㎖ for 12 hours showed the presence of perforations and irregular rod shaped with wrinkled surfaces.

음향 현미경을 위한 음향렌즈의 설계에 관한 연구

고대식,전계석 慶熙大學校 1987 論文集 Vol.16 No.-

In this paper, the design method of acoustic lens and its performance for the acoustic microscope have been studied and analyzed. It has been found that the focused transducer with acoustic lens attacted provide the acoustic beam intensity increased by π^2 compared with the plane piston transducer without acoustic lens. For the purpose of investigating the resolution of acoustic lenses and the contrast of acoustic images at frequencies of operation of 3 MHz and 10 MHz the focused spherical transducers of F/1.0 and F/0.7 have been employed and the Kevlar-epoxy composite and 10-won coin samples have been used in this experiment. It has been shown that the experimental results are in good agreement with theoretical simulation.

의사국가시험성적과의 상관관계로 살펴본 임상종합평가시험의 가치 : 2002년 연세대 원주의대 졸업생을 대상으로

김명수,김춘배,차병호,박기창,권상옥,신계철,이혜용,강성준,차봉석 한국보건의료인국가시험원 2004 보건의료교육평가 Vol.1 No.1

Korean Medical Licensing Examination(KMLE) 2002 focused on evaluation of the integrative medical knowledge such as primary clinical care or problem-solving competence. We analyzed the correlation among the year-wise student academic scores(grade score), trial examination scores and KMLE score by correlation analysis and multiple regression method. Four times of trial examination were taken in 2001, which were composed according to the principles of KMLE. Trial examination scores were significantly correlated with student grade scores (p(0.05). KMLE score also correlated with student grade score a nd trial examination score. The grade score at senior had higher correlation coefficient than the grade score at junior in correlation analysis. In multiple regressions, grade score at senior and mean score of trial examinatio n score were significant variants affecting KMLE score. Based on this result, regression formula such as [KMLE score] = 110.596+21.449^*[6th grade score of student] + 0.577^*[mean of trial examination score] was established (R2=0.764, p<0.001). Our results show that the trial examination is useful evaluation tool for final assessment of medical achievements. Also a trial examination is used as a reference data for student guidance and control in preparing for KMLE.

고정화 및 저장 온도에 따른 Photobacterium phosphoreum의 Bioluminescence 안정성의 변화

김현숙,정계훈,전억한 경희대학교 생명자원과학연구원 1998 硏究論文集 Vol.19 No.-

P phosphoreum의 고정화에 있어 중요한 것은 matrix의 선택이며, matrix로서 soudium alginate만을 사용하여 고정화 하는 것보다는 strontium chloride를 첨가하여 gel의 견고성을 높여주었을 때 세포의 bioluminescence 유지도가 증가하였다. 저장온도에 따른 세포의 bioluminescence 유지도와 활성도와 관련하여 -70℃, -20℃, 20℃에서 저장한 세포의 경우 저장 1일 후에 급격한 bioluminescence의 감소를 보였으며 낮은 세포 활성도를 보인 반면 4℃의 경우 bioluminescence의 유지도가 15일 이상 이어졌으며 높은 활성도를 나타내었다. 따라서 P phosphoreum의 bioluminescence 안정성에 있어 가장 좋은 결과를 나타낸 것은 2.5%(W/W) sodium alginated와 0.3M(W/V) strontium chloride로 고정화하여 4℃에 저장한 세포였다. The objective of this work was to unprove biolummescence stability of Photobacterium phosphoreum when stored at different temperature in view of developing contmuous on-line monitonng system for pollutants m environment. A long-term experiment was performed to determine how immobilization affects the mamtenance and stability of biolummescence from luminescent bacteria at appropnate temperature. The unmobhzed cells of P. phosphoreum were compared with free cells m terms of mamtenance of biolummescence at room temperature. It was found that the biolummescence of cells immobilized on strontium alginate showed higher biolummescence mtensity than both free and mixed cells with only algmate as a matrix. The effect of temperature on the biolummescence stability was investigated with free and immobilized cells stored at 20℃, 4℃, -20℃ and -70℃ for 20 days. Both free and immobilized cells stored at 4℃ emtted a stable biolummescence whde the biolummescence markedly decreased with those stored at 20℃, -20℃ and -70℃.