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Helicobacter pylori 편모 유전자의 클로닝 및 염기서열 분석
이광호,이우곤,조명제,도영미,백승철,강경희,박필성,이상룡 경상대학교 유전공학연구소 1993 遺傳工學硏究所報 Vol.12 No.-
A λgt11 expression libary of H. pylori DNA in E. coli Y1090 was screened with flagellin-specific rabbit antiserum for molecular cloning of the flagellin gene of H. pylori. A positive clone, λHPF4, was obtained and the recombinant antigen expressed from λHPF4 was a fusion protein with the molecular weight of 168kd. Sequence analysis of antigen-encoding DNA showed that an open reading frame composed of 1,536 nucleotides encodes a polypepride with a oredicted molecular size of 54kd. This open reading frame did not show the homology with flaA gene encoding 56kd protein of H. pylori and was confirmed as a unique sequence through homoligy searching. Therefore, the cloned antigen is supposed to be the carboxy-terminal region of the other flagellin protein of H. pylori, flaB, with the molecular weight of 58kd.
제7차 교육과정에 따른 산ㆍ염기 단원의 STS과학 교육에 적합한 CBL 실험 프로그램의 개발
이광필,김동화,최성호,류재정,이무상 慶北大學校 師範大學 科學敎育硏究所 2001 科學敎育硏究誌 Vol.25 No.-
This study developed the 'advanced-supplementary' course experimental program with CBL(Calculator-Based Laboratory) system in conformity with the reform of the 7th science curriculum and applied the CBL experimental program to 'acid-base' chapter of middle-high school. The results of this study shows that a practical use of CBL system as powerful instrument can be applied to experiment program of 'acid-base' chapter in order to causes learner's motivation and enlarge a sphere of inquiry-activity in science.
치과 치료 경험이 아동의 치과의사와 치과 치료에 대한 태도에 끼치는 영향에 관한 조사 연구
허용욱,이광희,문필성,김대업 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.2
The purpose of this study was to investgated the effect of dental experience the attitude of children toward the dentist and the dental treatment.The subject of the study were 1,090 children from second grade to forth grade of three elementary schools of the Iksan city.The children were investgated by the questionnaire which cosisted of seven items about the attitude toward the dentist,the attitude toward the dental treatment,the dental treatment experience,the number of times of dental treatment,the last time of the dental visit,pain of dental treatment,and fear of dental treatment. There were significant differences among the grades in the attitude toward the dental treatment and in the attitude toward the dentist(P<0.01).Second grade children had the most negative attitudes.But,there were no differences between boys and girls.Girls felt more pain and fear than boys(P<0.01).There were significant relationships between the pain and the attitude toward the dentist,between the pain and the attitude toward the dentist, and between the fear and the attitude toward the dental treatment(P<0.01,all).The number of dental visit had significant relationship with the attitude toward the dental treatment(P<0.05).There was significant relationship between the pain and the fear between the attitude toward the dentist and the attitude toward the dental treatmen.
Kwang-Pill Lee,Gopalan, A.I.,Manesh, K.M.,Santhosh, P.,Kyu Soo Kim IEEE 2007 IEEE TRANSACTIONS ON NANOTECHNOLOGY Vol.6 No.3
<P>Electrospun membranes of poly(vinylidene fluoride-co-hexafluoropropylene) (PVdF-HFP)/multiwall carbon nanotube (MWCNT) composite are prepared and loaded with lithium salts from electrolyte solution. Field emission transmission electron microscopy provides evidence for the uniform distribution of MWCNTs into the matrix of PVdF-HFP. The interconnected morphology as evident from field emission scanning electron micrograph forms the path for the lithium ion conduction. Results from electrochemical impedance spectroscopy inform that the presence of MWCNTs in PVdF-HFP matrix improves interfacial stability between lithium electrode and membrane and augment conduction path in the polymer electrolyte membrane. Further results from impedance measurement reveal the contribution of MWCNTs toward conductivity. A prototype cell is fabricated with PVdF-HFP/MWCNT as polymer electrolyte. The electrospun PVdF-HFP electrolyte membrane with 2% MWCNTs content shows an ionic conductivity of about 5.85 mSmiddotcm<SUP>-1</SUP> at 25 degC. Also, PVdF-HFP/MWCNT electrolyte membrane exhibits good electrochemical and interfacial stability and can be potentially suitable as electrolyte in lithium ion secondary battery</P>