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무기 이소시안화 및 티오시안화 전이금속 화합물의 구조에 관한 연구
박윤창,지광용,이미녕,이영희 성균관대학교 기초과학연구소 1989 論文集 Vol.40 No.1
IR, UV 그리고 EPR 분석을 통해 〔R_4N〕_2 〔M(NCX)_4〕 (이 때, R=CH_3^+, C_2H_5^+; M=Cd Cu, Zn, X=O, S) 화합물의 착이온에서 중심 금속이 N과 결합하고 있는지 아니면 O, S와 결합하고 있는지를 연구했다. 위의 모든 화합물은 금속이 N과 결합된 것으로 규명되었다. Cd(Ⅱ)과 Cu(Ⅱ) 화합물은 일그러진 정사면체 구조에 의해 C-N, C-S 신축진동수의 분리를 보였다. IR 스펙트럼에서 〔(C_2H_5)_4N〕_2 〔Cu(NCS)_4〕는 단일선의 C-N 신축진동수를 보인 반면, 〔(CH_3)_4N〕_2 〔Cu(NCS)_4〕는 일그러진 정사면체 구조에 기인한 이중선을 보였다. 〔(C_2H_5)_4N〕_2 〔(Cu(NCS)_4〕는 d-d 전이로 판단되는 넓은 전자흡수띠가 438nm 근처에서 관찰된 반면, 〔(CH_3)_4N〕_2 〔Cu(NCS)_4〕는 d-d전이에 의한 흡수띠가 관찰되지 않았다. 5% Cu(Ⅱ)이 혼입된 〔(C_2H_5)_4N〕_2 〔Cd(NCS)_4〕의 EPR 스펙트럼은 비등방성 구조를 지녔으나, 〔(CH_3)_4N〕_2 〔Cd(NCS)_4〕는 비등방성 뿐만 아니라 정규적인 정사면체 구조의 증거인 등방성을 상온에까지 지녔다. IR, UV and EPR spectra of the compounds whose formulas are 〔R_4N〕_2 〔M(NCX)_4〕, where R=CH_3^+, C_2H_5^+ and M=Cd, Cu, Zn;X=O, S have been studied in order to investigate whether the isocyanato, thiocyanato group is bonded to the central metal through the nitrogen atop or through the sulfur, oxygen atom in those complex ions. All above complexes showed that the metal is linked through the nitrogen atom. It was turned out to be that. the splitting in C-N and C-S stretching frequencies of Cd(Ⅱ) and Cu(Ⅱ) compounds is due to the distorted tetrahedral structure of those compounds. While IR spectrum of 〔(C_2H_5)_4N〕_2 〔Cu(NCS)_4〕 showed singlet C-N stretching frequency, 〔(CH_3)_4N〕_2 〔Cu(NCS)_4〕 showed doublet which indicates that the compound has a distorted tetrahedral structure. 〔(C_2H_5)_4N〕_2 〔Cu(NCS)_4〕 was observed to have a broad electronic absorption band near 438 nm which originates from d-d transition. On the other hand, 〔(CH_3)_4N〕_2 〔Cu(NCS)_4〕 did not show an absorption band due to d-d transition. EPR. spectrum of Cu(Ⅱ) doped 〔(C_2H_5)_4N〕_2 〔Cd(NCS)_4〕 showed anisotropic structure and 〔(CH_3)_4N〕_2 〔Cd(NCS)_4〕 showed both anisotropic and isotropic structure even at room temperature which is an evidence of regular tetrahedral structure.
이상녕(Sang Nyeong Lee),이송은(Song Eun Lee),배미옥(Mi Ok Bae),남광일(Kwang IL Nam),박광성(Kwang-Sung Park),배춘상(Choon Sang Bae),김백윤(Baik Yoon Kim),박성식(Sung Sik Park),안규윤(Kyu Youn Ahn) 대한해부학회 2005 Anatomy & Cell Biology Vol.38 No.3
당뇨병을 가진 여성의 주된 성적문제는 질 윤활작용의 감소와 성적 각성장애이고 이는 질 조직의 섬유화와 관련이 있다고 한다. 본 연구는 streptozotocin (65 mg/kg)투여 당뇨유발 군과 당뇨유발 후 insulin 투여 군 흰쥐에서 질 조직의 변화를 조직화학, Western분석 및 면역조직화학 기법을 통해 관찰하고자 하였다.평균 혈당농도는 대조군에서 79±16 mg/dL, 당뇨유발 군에서 453±188.4 mg/dL 그리고 insulin 투여군에서 56.7±20.6 mg/dL 였다. H-E 소견에서 대조군의 질벽은 6~11층의 중층편평상피, 민무늬근육, 결합조직 및 다수의 혈관들로 구성되어 있었다. 당뇨유발 군에서는 중층편평상피 층이 2~6층으로 감소하였으며 또한 민무늬근육과 혈관의 수와 크기도 감소하였다. Insulin 투여 군은 대조군의 소견과 유사하였다. 대조군의 Masson’s trichrome 염색소견에서는 보라색으로 염색되는 민무늬근육이 상당량 관찰되었고 청색으로 염색되는 아교섬유는 규칙적으로 분포되었다. 당뇨유발 군에서는 아교섬유가 증가되어 성긴결합조직이 치밀결합조직으로 변하였으며, 아교섬유의 배열도 불규칙하였다. Insulin 투여 군은 대조군의 소견과 유사하였다. Western분석 결과 당뇨유발 군에서의 TGF-β1의 발현은 현저히 증가하였으나 Ec-NOS의 발현은 감소하였다. TGF-β1에 대한 면역양 성 반응은 섬유모세포와 아교섬유에서 관찰되었고 당뇨유발 군에서 Western분석 소견과 같이 현저히 증가하였으며 대조군과 insulin 투여 군에서는 미약하였다. Ec-NOS에 대한 면역양성 반응은 혈관의 내피세포에서 관찰되었으며, 면역반응성은 Western분석 소견과 같이 대조군과 insulin 투여 군에서는 현저하였으나 당뇨유발 군에서는 상당히 감소하였다. 대조군과 insulin 투여 군의 estrogen receptor α에 대한 면역양성 반응은 중층편평상피의 바닥층과 중간층, 민무늬근육세포 및 신경에서 관찰되었으나 당뇨유발 군에서는 중층편평상피의 중간층, 민무늬근육세포 및 신경에서만 관찰되고 반응성도 감소하였다. 이상의 결과로 미루어 당뇨유발 흰쥐 질 조직의 섬유화는 TGF-β1, NOS 및 estrogen 등과 관련이 있을 가능성을 제시하였으며, 이로 인한 당뇨 여성의 성기능 장애는 혈당을 조절함으로써 예방할 수 있음을 암시하였다. The most commonly reported sexual problems in diabetic women are sexual arousal disorder and a lack of vaginal lubrication. The aims of this study were to investigate the vaginal structural changes and expressions of TGF-β1, Ec-NOS and estrogen receptor α by histochemistry, immunohistochemistry and Western blot analysis in diabetic and insulin-treated diabetic rats. The mean blood glucose levels were significantly increased in the diabetic rats (453±188.4 mg/dL) compared to the control group (79±16 mg/dL) and insulin-treated diabetic rats (56.7±20.6 mg/dL). The vaginal wall in control rat showed 6~11 layered stratified squamous epithelial lining and submucosal smooth muscle, connective tissue and vasculatures. In diabetic rat, the vaginal epithelium was reduced to 2~6 layers and the submucosal vasculatures were decreased in size and number. Collagen fibers were increased and irregularly distorted arrangement. Insulin-treated diabetic rat showed similar morphologic features as control rat. In diabetic rat, TGF-β1 expression was upregulated by 1.65 times and Ec-NOS expression was 40% downregulated compared to control and insulin-treated diabetic rats in Western blot analysis. In control and insulin-treated diabetic rats, TGF-β1 immunoreactivity was detected in fibroblasts and the collagen fibers, Ec-NOS immunoreactivity in the endothelial cells of blood vessels, and estrogen receptor α immunoreactivity in the basal and intermediate cell layers of stratified squamous epithelium, smooth muscle fibers, and nerve fibers. In diabetic rat, expression of TGF-β1, Ec-NOS, and estrogen receptor α was exhibited comparable cellular patterns of labeling, but signal intensity was increased in TGF-β1 and decreased in Ec-NOS and estrogen receptor α. These results suggest that vaginal tissue fibrosis in diabetes mellitus may be caused by altered expression of TGF-β1, NOS and estrogen. It also implies that sexual arousal disorder and lack of vaginal lubrication in the diabetic women could be protected or delayed by controlling blood glucose levels.
실내식물 개발을 위한 차광처리가 자생 후추등(Piper kauzura)의 생육에 미치는 영향
방광자(Bang Kwang-Ja),주진희(Ju Jin-Hee),김중녕(Kim Joong-Nyeong),이상옥(Lee Sang-Ok) 한국실내조경협회 2003 室內造景 = Journal of the Korean institute of interior Vol.5 No.2
This study was conducted to investigate the growth responses of Piper kauzura as influenced by 4 different shading (Control, 50%, 70%, 90%). Under 50 or 70% shading, plant height, number of leaf increased compared with the other shading except leaf length, leaf width, petiole length, internode length. The plant grown under Control was shown smaller and more yellowish green, leaf color and under 90% shading growth was a little inferior to those under the 50 or 70% shading. Chlorophyll contents were increased in proportion to the lowing light intensity. Thus, 50 or 70% shading Piper kauzura seemed to be maintained highly valued growth.
Nayeong Kim,Kwang-Nyeong Lee,Mingeun Sagong,Gyeong-Beom Heo,Youn-Jeong Lee 한국예방수의학회 2022 예방수의학회지 Vol.46 No.4
Wild birds, especially aquatic birds, are the natural reservoir of avian influenza virus (AIV), and many kinds of water body can be contaminated with feces of these birds. Seasonally, AIVs can be dissolved in the environmental water from the feces of the infected birds, and this water can be a target for viral detection and identification. In this study, we employed and tested three different filters for concentrating AIV, and it was shown that high concentration factor in terms of viral density could be achieved with viral samples diluted with natural water. Wild bird fecal samples containing low pathogenicity H5 AIVs were successfully concentrated with the adsorption and elution method using mixed cellulose esters membrane; the recovery rate of virus was 35.5 % and the concentration factor was about 50 on average. For the larger volume of water sample, we proved that an inline disposable filter with high surface area, 300 cm2, has a comparable concentration factor to the adsorption and elution method and the filter could be used in the field conveniently by being plugged into peristaltic pump. These validated methods for water sampling may be used as a supplementary for virological surveillance on wild migratory birds or during the epidemiological investigation on the environment near affected premises by AIV.
Control of Foot-and-Mouth Disease during 2010–2011 Epidemic, South Korea
Park, Jong-Hyeon,Lee, Kwang-Nyeong,Ko, Young-Joon,Kim, Su-Mi,Lee, Hyang-Sim,Shin, Yeun-Kyung,Sohn, Hyun-Joo,Park, Jee-Yong,Yeh, Jung-Yong,Lee, Yoon-Hee,Kim, Min-Jeong,Joo, Yi-Seok,Yoon, Hachung,Yoon, Centers for Disease Control and Prevention 2013 Emerging infectious diseases Vol.19 No.4
<P>An outbreak of foot-and-mouth disease caused by serotype O virus occurred in cattle and pigs in South Korea during November 2010–April 2011. The highest rates of case and virus detection were observed 44 days after the first case was detected. Detection rates declined rapidly after culling and completion of a national vaccination program.</P>