백서 심근경색모델에서 시간경과와 경색의 크기에 따른 심자도의 변화

김미성(Mi Sung Kim),박영선(Young Sun Park),권순길(Sun Gil Kwon),지정훈(Jeong Hoon Ji),신종성(Jong Sung Shin),오광식(Kwang Sik Oh),양용모(Yong Mo Yang),연태진(Tae Jin Youn),김동운(Dong Woon Kim),조명찬(Myeong Chan Cho),이용호(Yong Ho 대한내과학회 2002 대한내과학회지 Vol.62 No.1

N/A Background: Magnetocardiogram (MCG), which records the changes of magnetic fields generated by the heart`s electrical activity, theoritically can provide unique data for clinical application. To date, MCG has been investigated only at a single time point after myocardial infarction (MI) with severe left ventricular dysfunction in rats. The purpose of the present study was to investigate sequential changes of MCG after MI and to evaluate effects of infarct size on MCG. Methods: Acute MI were induced by the permanent ligation of left coronary artery in 22 rats. Magnetic fields were recorded just above a rat with Nb Superconducting Quantum Interference Device (SQUID) gradiometer inside a magnetically shielded room. MCG was measured before and immediately after surgery and it was subsequently recorded at the time points of 1, 4 and 6 hours post operatively. MCG was also measured at 1, 3, 7 and 21 days after surgery. Results: Elevation of ST segment and appearance of pathological Q wave on the MCG were evident immediately after the ligation of coronary artery and persisted to 6 hours after MI. On MCG, ST segment was depressed and T wave was inverted from 1 day after MI. In rats with small-and moderate- sized MI (infarct size〈30%), ST depression returned to near the isoelectric level and Q wave disappeared from 7 days after MI. However, ST depression and Q wave were still present in rats with larger infarct (infarct size≥30%). Conclusion: Evolutional changes of MCG were well-recognized up to 21 days after MI. Furthermore, the infarct size can be expressed by the extent of Q wave and ST segment depression on MCG. Taken together, these data indicate that MCG is a helpful modality for the diagnosis, evaluation of infarct size and follow up after MI.(Korean J Med 62:42-48, 2002)

포장재질에 따른 김치의 저장성Ⅰ

강미정,윤경영,이광희,윤광섭,김광수 대구효성가톨릭대학교 식품과학연구소 1997 식품과학지 Vol.9 No.-

수출용 김치에 주로 사용되고 있는 유리병을 대상으로 유통과정 중 상품성 유지와 더불어 저장성 향상을 도모하고자 포장방법에 따른 저장성 효과를 비교ㆍ실험하였다. 포장방법은 유리병을 이용하여 밀폐포장과 투과성 포장을 하였으며 내부 포장재로 사용한 PE film 두께별(0.01, 0.06, 0.08, 0.1㎜) 저장성 향상능력을 비교하였다. 포장김치는 10℃에서 28일간 저장하면서 pH 및 산도, 색도의 변화와 관능적 품질변호를 측정하였다. 저장기간에 따른 pH와 산도의 변화에서 포장방법간에 큰 차이는 없었으나 밀폐 포장의 경우 film 두께가 다소 얇은 0.06, 0.01㎜ 처리구에서, 투과성 포장의 경우 film 두께가 다소 두꺼운 0.06, 0.08㎜ 처리구에서 높은 pH와 낮은 산도를 나타내었다. 특히 0.06㎜ 처리구의 경우 포장방법에 관계없이 control이나 타처리구에 비해 완만한 pH와 산도변화를 나타내었고, 상미한계점이 연장될 뿐만 아니라 효모에 의한 연부현상이나 탄산가스에 의한 뚜껑의 개봉이나 폭발음 등의 현상이 나타나지 않았다. 색도 측정시 저장 14일을 전후로 증가하던 L, a, b값이 감소하는 경향이었으며 이는 밀폐 포장과 투과성 포장에서 동일하게 나타났다. 관능적 품질평가에서 포장방법간에 큰 차이는 없었으나 신맛과 조직김이 유의적인 관계가 있는 것으로 보였고, 상미한계점을 경과할수록 조직감이 기호도를 좌우하는 것으로 나타났다. 또한 0.06㎜ 투과성 포장시 밀폐 포장보다 신맛이 좋은 것으로 나타났다. 저장 14일 이후부터는 0.06㎜ film 처리시 관능적 품질평가 전반에 걸쳐 우수한 것으로 인정(5%의 범위)되었다. 이상의 결과로 유리병포장의 경우 기체투과성이 높은 PE film으로 내부포장하는 것이 저장성 연장효과를 기대할 수 있으며, 특히 0.06㎜ film으로 밀폐 포장 또는 투과성 포장시 높은 저장성 연장효과와 더불어 탄산가스에 의한 팽창방지를 완화시킬 것으로 기대된다. The effect of various packaging methods on kimchi quality was investigated in order to develop the packaging techniques for preventing bottled kimchi products from inflation and expolsing, due to fermentative gas evolved during storage and distribution. Kimchi was packaged in bottle(400g) under atomosphere different methods; 1) control, 2) airtight packaging, 3) permeable packaging, Kimchi packaged with polyethylene(PE) film was stored at 10℃ and film thickness were used 0.01, 0.06, 0.08, and 0.1㎜. To evaluated quality change of kimchi, pH, acidity, color, and sensory score were measured periodically. Although pH and acidity change was not significant depending on the packaging method, airtight packaging with 0.06, 0.01㎜ film and permeable packaging with 0.06, 0.08㎜ film showed higher pH value and lower acidity then those of the others. Color change at kimchi as L, a, b value increased until 14 days and the decreased in airtight and permeable packaging. Sensory evaluation were not significant among packaging method during storage, but sour taste and texture scores were significant. Also, sour tast of permeable packaging with 0.06㎜ film showed higher than airtight packaging. After 14 days of storage, total sensory evaluation was excellent of packaging with 0.06㎜ film. Therefore, 0.06㎜ was found to be the most effective packaging method, although airtight and permeable packaging were not significant for storage properties.

김치의 제조 및 유통중 가스발생 방지에 관한 연구

윤경영,강미정,이광희,윤광섭,김광수 대구효성가톨릭대학교 식품과학연구소 1997 식품과학지 Vol.9 No.-

대량생산을 위한 김치의 산업화에 있어서 가장 큰 문제점인 유통시의 산패현장과 포장팽대 및 파손을 지연시켜 김치의 저장기간을 연장하고자 이산화탄소 흡수능력을 가진 물질을 가스흡수제로 처리하여 저장중 김치의 품질을 검토하였다. 품질평가를 위한 측정항목은 pH, 산도, 색상, 이산화탄소 함량 및 젖산균수 그리고 총균수를 측정하였다. 수산화칼륨, 수산화칼슘, 수산화나트륨과 같은 알칼리물질과 연화마그네슘, 염화칼슘과 같은 염화합물을 가스흡수제로 사용한 결과, 수산화칼륨과 수산화나트륨처리구가 타처리구에 비해 pH의 감소가 적었으며, 또한 산도의 증가가 다소 낮았다. 수산화칼륨과 수산화나트륨을 각각 0.25%, 0.5%, 0.75% 세단계의 농도로 처리한 결과 pH와 산도에 있어서 수산화칼륨처리구가 수산화나트륨처리구에 비해 높은 pH와 낮은 산도를 나타내었다. 또한 각 처리구의 농도에 따라 큰 차이가 나타나지 않아 간편성과 경제적인 면, 그리고 시각적인 면을 고려해 볼 때 0.25% 수산화칼륨처리구가 탄산가스흡수에 의해 김치의 저장기간을 연장하는데 다소 효과를 보였다. PE bag으로 포장된 수산화칼슘과 PE flim(0.02㎜)으로 포장한 0.25% 수산화칼륨을 가스흡수제로 사용하여 무처리구와 비교하여 저장중 김치의 품질을 검토하였다. 저장중 0.25% 수산화칼륨처리구와 시판용 김치의 가스흡수제인 수산화칼슘처리구를 비교할 때, 수산화칼륨처리구가 무처리구에 비해 pH 및 산도의 차이가 컸으며, 김치의 조직과 국물속에 용해되어 있는 이산화탄소의 함량도 적었다. 그리고 총균수에 있어서 타처리구에 비해 수산화칼륨처리구가 높은 값을 나타내었다. When kimchi was manufactured and circulated, largest question is rancidity phenomenon and packing swelling. This study was conducted to prevent these phenomenon and prolong the shelf-life of kimchi. KOH, NaOH, Ca(OH)_2, MgCl_(2), and CaCl_(2) were used to gas-absorbent. The pH of kimchi used KOH, NaOH was higher than the others. The acidity of kimchi used KOH, NaOH was lower than the others. The concentration of KOH, NaOH was 0.25%, 0.50%, 0.75%. The decrease of pH and increase of acidity that kimchi dealt with KOH were lower than kimchi used NaOH. There in no significant difference in concentration of gas-absorbent. Comparision of kimchi used 0.25% KOH and Ca(OH)_2. Ca(OH)_2 packaged PE bag and 0.25% KOH packaged PE film(0.02㎜) were used to gas-absorbent. On comparision to control, kimchi dealt with 0.25% KOH was higher than kimchi used Ca(OH)_2 at pH and acidity. The CO_(2) contents of kimchi dealt with 0.25% KOH was lowest. The number of total microbe kimchi dealt with 0.25% KOH was highest.

귀인 훈련이 경도 정신지체아동의 무력감과 학업성취 및 태도에 미치는 효과

여광응,구유미,이정현,김선미 한국특수교육문제연구소 2001 특수교육저널 : 이론과 실천 Vol.2 No.1

이 연구의 목적은 경도 정신지체아동의 학습된 무력감을 감소시키기 위하여 체계적인 귀인훈련를 적용함으로써 학업성취능력과 학습태도 개선에 미치는 영향을 밝히는데 있다. 연구대상은 개인용 지능검사 결과 IQ 60-75사이이고 초등학교 특수학급에서 시간제 특수교육을 받고 있는 이동 3 명으로서 신체 및 언어장애와 같은 심한 장애가 없는 아동이다. 연구결과, 귀인훈련이 오랜 학교학습상황에서 겪게 된 학습의 실패상황에서 실패원인을 「능력」에 귀인하는 아동을 「노력」 에 귀인하도록 힘으로써 아동의 「학습된 무력감」 를 감소시키는데 효과적임이 밝려졌다. 즉,뺄셈학습과제의 수행속도가 귀인훈련 후 빨라졌으며 과제에 대한 흥미도 많이 증가하였다. 또한 귀인훈련이 경도 정신지체 아동의 학습태도를 개선하였다. 귀인훈련 후 아동의 실패과제에 대한 지속시간이 많이 늘어났으며, 아울러 실패과제에 대한 불안행동이 많이 감소하였고, 주의집중이 향상되며 학습태도가 바람직히게 변화된 것으로 나타났다. 이 결과는 귀인훈련을 통해 경도 정신지체아동의 학업성취도와 학습태도에 긍정적인 변화를 가져올 수 있음이 확인되었다. The purpose of the present study is to investigate how systematic atributional training affects leaming achievements and leaming attitudes for the children with mild mental retardation through decreasing their feelings of learned helplessness. For the study, three children with mental retardation, whose 1Qs range from 60 to 75, were selected from those who were enrolled at special classes of elementary schools. They do not have physical handicapped and language problems. The procedure applied to this study focused on the changes the reason for the learning failure from "their ability" to "their lack of effort".

형제의 지위변인 및 형제관계와 아동의 친구간 갈등해결전략과의 관계

박미진,김광웅 숙명여자대학교 아동연구센터 2002 兒童硏究 Vol.15 No.2

The purpose of this study is to find some relationships among sibling status factors; the order of the births, the sex distribution of the brother/sister and the age gap between them, sibling relationship and conflict resolution strategies in children's friendship. The subjects of this study were 482 male and female children, made up of the 5th and 6th grade elementary schoolers with a single brother. In this study, Sibling Relationship Questionnaire developed by Furman and Buhrmester(1985) were used to observe the sibling relation. A Scale on Conflict Resolution Strategies in Children's Friendship(Rhee, Koh, & OH) was used to measure children's conflict resolution strategies in children's friendship. The data was analysed by using one-way ANOVA, and stepwise regression. The results of this study were as follows: First, in the result of having seen the difference of children's conflict resolution strategies according to their birth order, which is difference in dominating and obliging strategies. It was shown that the first-born children and only child uses dominating strategies the most but only child uses less obliging stategies than the firts-born children. Second, in the result of having seen the difference of the children's conflict resolution strategies according to compromising-integrating and obliging strategies, which is their birth order and the sex distribution. It was shown that female children having brother were more willing to used compromising-integrating strategies. But male children having sister and only child weren't used. Also female children having elder brother, they were more willing to used obliging strategies. Third, in the results of having seen the difference in the conflict resolution strategies for children's friendship according to the age gap, it was shown that no difference. Finally, Sibling relationship has a lot to do with the conflict resolution strategies in children's friendship. Most of all, kinship and friendship might be one of explanatory factors in compromising-integrating strategies, avoiding strategies and obliging strategies while confliction appeared to be the factor in the dominance strategies. More brotherhood or sisterhood is friendly and close, one deal with the conflictions among one's friends in terms of compromising-integrating avoiding and obliging strategies, and more conflicting one's brotherhood relationship one use dominance strategies mostly.

초등학생을 위한 사이버 커뮤니티의 교육적 활용 방안에 관한 탐색적 연구

한광현,김미량 한국컴퓨터교육학회 2002 컴퓨터교육학회 논문지 Vol.5 No.2

많은 수의 초등학생들이 인터넷을 다양한 방법으로 활용하고 있으며 그 중 커뮤니케이션을 위한 수단으로도 활용하고 있다. 특히 면대면의 학교 상황에서 여러 요인으로 인해 원활하지 못한 상호작용을 촉진시키기 위한 사이버 학급커뮤니티의 활용사례도 급증하고 있다. 본 논문에서는 초등학교 사이버 커뮤니터의 관찰과 분석을 통해 사이버 커뮤니티의 교육적 활용 방안을 제시하고자 하였다. 주요 결과로서는 커뮤니티를 효율적으로 활용하기 위해서는 교사가 커뮤니티의 활용 방향성에 대한 목표를 설정하고, 채널 특성에 대한 사전 탐색이 필요하다는 것과, 학생 자율권과 교사 권한을 적절하게 조화시켜 운영해야 한다는 점을 들 수 있다. 또한 사이버 공간에서의 상호작용으로만 그치지 않고 온라인과 오프라인이 상호 유기적으로 운영되어야 하며, 누적물은 학생들의 포트폴리오로서 학기말에 학생들에게 제공될 때 효과적일 것이라는 결론도 도출되었다. Cyber school community appears to have essentially emerged from the needs for interactive communication, which would not be easy in normal school environments. Message board, chatting rooms and e-mails allow students to connect with each others, share news, find ways to help study, or post personal stories. The purpose of this paper is to give a set of guidelines, based upon the direct observations and questionnaire analysis, for utilizing the cyber school community of elementary school children. The following conclusions are provided; the appropriate goal needs to be set before the cyber school community is to be developed: the balance between the off-line and on-line interaction needs to be maintained; actions for motivating students to participate in the cyber community need to be taken by teachers.

미나리과 산채의 아질산염 소거능

노광석,양미옥,조은자 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.1

The nitrite scavenging effect of three kinds of edible wild plants belong to Umbelliferaeceae family was investigated. The contents of crude protein, crude lipid and β-carotene of the plants were in the range of 22.99∼31.00%, 3.51∼5.08% and 5.40∼19.81 ㎎%, respectively. The content of ascorbic acid was very low and selenium was not detected in any samples. The contents of linoleic acid and linolenic acid were 66.46∼77.44%. The content of total phenol was 875.77 ㎎% in Ledebouriella seseloides WOLFF, which was the highest among the samples. The methanol fraction of the plant extract showed higher nitrite scavenging effect than the water fraction. The nitrite scavenging effects of the samples were the highest at pH 1.2 and lowest at pH 6.0, suggesting that it's pH dependent. Nitrite scavenging effect of methnol-soluble fractions obtained from Ledebouriella seseloides WOLFF was 95.3% at pH 1.2, which was the greatest among tested samples. There was a high correlation between the nitrite scavenging effect and the total phenol content.

소구치 발치에 의한 교정치료후의 치열궁 형태 및 크기에 관한 연구

이승미,윤영주,김광원 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.5

본 연구는 4개의 제 1소구치를 발치하여 치료된 1급 부정교합 증례의 치열궁 형태와 크기를 실제 임상에서 이용하기 용이 하도록 아치와이어가 지나는 브라켓 슬럿 레벨에서 알아보고자 시행하였다. 이를 위해 치아 크기가 정상범주에 있으면서 4개의 제1소구치를 발치하여 교정 치료후 비교적 양호한 결과를 보인 모형 60개(남자 30개, 여자 30개)를 대상으로 계측점과 계측선을 설정하고, 통계 처리 하여 다음과 같은 결론을 얻었다. 1. 아치와 와이어가 지나는 브라켓 슬럿 레벨에서 남녀의 상하악, 협설측 치열궁 형태를 얻었다(Fig 5,6,7,8). 2. 남녀별 상하악, 협설측 평균 악궁형태에 대한 포물선식 및 변수의 적합도를 나타내는 결정 계수를 구하였다(Table 5). 3. 치열궁 크기를 나타내는 대부분의 선계측 항목에서 남자가 여자가 큰 수치를 보였으며, 통계학적 유의차를 보인 항목은 상악의 견치 폭경, 제 1대구치 폭경, 제 2대구치 폭경, 소수치 고경, 제 2대구치 고경과 하악의 견치 폭경, 제 2대구치 폭경, 소구치 고경이다(p<0.05, p<0.01) 4. 상하악의 제 1대구치간 폭경의 차이는 남자에서 6.3mm, 여자에서 6.05mm이었다. This study was done to estimate arch forms and dimensions at the bracket level where archwire was placed in Angle's Class I first premolars extraction cases. 60 post-treatment dental casts which had attained good orthodontic treatment result were used in this study. Many landmarks and linear measurement items to describe arch forms and dimensions were determined and measured. With a computer system and digitizer, arch forms were described and linear measurement items were statistically analysed. The following results were obtained. 1. The average labial and lingual arch form at the bracket level were obtained. 2. Arch forms were expressed by parabolic equations and coefficients of determination. 3. Arch widths were larger in male than in female. 4. There were statistical significances in upper intercanine width, upper interfirst molar width, upper intersecond molar height, lower intercanine width and lower interfirst molar width between both sexes (p<0.05, p<0.01) 5. Interfirst molar width differences between maxilla and mandible were 6.43mm in male and 6.05mm in female.

사람과 설치류간의 체세포 융합세포 개발과 사람유전자 지도작성을 위한 융합세포의 응용

진미욱,강윤성,이광호 中央大學校 基礎科學硏究所 1998 基礎科學硏究所 論文集 Vol.12 No.-

Human-rodent somatic cell hybrids have proven very useful in mapping genes and DNA sequences to specific chromosomes. Our experiment was directed to construct a human chromosome panel which consists of hybrid cell lines containing only a human chromosome or a few. Forty seven hybrid clones were analyzed for contained human chromosomes by G-, Q-banding but rearranged fragments of human chromosome could not be characterized by these methods. To overcome these problems, we performed IRS-PCR-FISH(interspersed repetitive sequence-PCR-FISH). To amplify the human specific DNA sequences in human-rodent somatic cell hybrids, primers directed at human-specfic regions of Alu-517(SINE) and Ll-Hs(LINE) were used. FISH using the biotin-labelled products made it possible to identify individual human chromosomes and rearranged human materials in hybrids, which were not identifiable by conventional cytogenetic methods. Thus, IRS-RCR-FISH allowed us to characterize human chromosomes in hybrid cells to generate the human chromosome panel. Unknown genes could be mapped to specific chromosmes by biochemical and molecular techniques such as enzyme and southern blot analysis combining with this panel. This panel will serve as a useful resource for mapping and cloning of novel genes.

교정환자의 브라켓과 치아 경계부에 존재하는 치면세균막내 mutans streptococci 종 및 생물형의 식별

김미애,유소영,김화숙,국중기,임성훈,윤영주,김광원 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.1

본 연구는 교정환자의 브라켓과 치아 경계부 및 브라켓으로부터 2 mm 이상 떨어진 치아 평활면의 치면세근막에 존재하는 mutaus streptococci의 종 및 생물형에 차이가 있는지를 알아보고자 시행되었다. 조선대학교 치과병원에 내원한 13세 이상 35세 미만의 환자 28명으로부터 브라켓을 장착하고 있는 61개 치아에서 치균세균막을 채취하여 mutans streptococci를 MSB 배지에서 선택적으로 분리한 다음, 이들의 지놈 DNA를 추출하여 dextranase 유전자를 표적으로 하는 중합효소연쇄반응법을 시행하고, 그 증폭물을 Hae Ⅲ로 소화하고, 이를 전기영동하여 제한효소절편길이에 따라 그 종을 식별하였다. 또한 생물형을 조사하기 위하여 생화학적 검사를 실시하였다. 그 결과 브라켓과 치아 경계부 및 브라켓으로부터 2 mm 이상 떨어진 평활면의 치면세균막에 존재하는 mutans streptococci 종은 서로 비슷한 검출 빈도를 보이나 두 곳에 존재하는 mutans streptococci 생물형은 서로 차이가 있는 것으로 나타났다. 향후 브라켓과 치아 경계부 및 치아 평활면의 치면세균막의 mutans streptococci 생물형의 차이와 브라켓 주위의 법랑질 탈회 및 치아우식증 발병과의 상관관계에 대한 연구가 필요하다. The aim of this study was to compare the species and biotypes of mutans streptococci isolated from dental plaques sampled from the interfaces between the bracket and tooth surface and smooth tooth surfaces in orthodontic patients. Dental plaque was collected from the interfaces between brackets and teeth (test group), and from smooth tooth surfaces distant from brackets by more than 2 mm (control group). The dental plaque collected by a sterilized curette was transferred into a vial of 1 X PBS. The sample in the vial was vigorously vortexed for1 min and plated on mitissalivarius bacitracin (MSB) agar plate using cotton tips. The agar plates were incubated at 37℃ in a candle jar for 2 days, and again incubated for 1 more day at anambient temperature. Individual colonies were cultured in TH broth at 37℃ CO₂ incubator. The PCR-RFLP based on dextranase gene was performed for the identification of mutans streptococci at the species-level. For biotyping of mutans streptococci, biochemical tests were performed. There was no significant difference of the species of mutans streptococci isolated from both test and control groups. However, the biotypes of the mutans streptococci isolated from test and control groups were different. These results may offer the basic data to verify the relationship between the mutans streptococci biotype and enamel decalcification or dental caries in orthodontic patients with fixed appliances.