Application of different cell disruption methods for lipid extraction from Botryococcus braunii

Kwang?kuk Cho,Hai?Linh Tran,Choul?Gyun Lee 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

炭素鋼의 摩擦熔接에서 熔接面의 形狀에 관한 硏究

鞠賢雨,閔宅基,金光日,金茂成 忠南大學校 産業技術硏究所 1994 산업기술연구논문집 Vol.9 No.2

This report deals with a study on figure of friction welding of carbon steel. It is investigated to vary figure. Main result obtained in this study are as follows; 1) In friction welding of carbon steel, the optimization of figure experiments on several type [C(120 )>A(180 )>B(150 )>D(90 )type]. The optimization of figure is C-type(120 ) 2) The tensile strength under 1.0-1.4sec heating time is shown 43.2kgf/㎟, which is the higher than base metal (42kgf/㎟) in C-type. 3) The horizontal hardness is similar to base metal. Heat affected zone where hardness is lower than base metal spreads 3mm far from the friction weld interface in C-type

초박막 ONO 구조의 TDDB 특성

국삼경,서광열 광운대학교 신기술연구소 1998 신기술연구소논문집 Vol.27 No.-

터널링 산화막과 블로킹 산화막의 두께가 각각 23A˚. 40A˚으로 동일하고 질화막이 45A˚, 91A˚, 223A˚으로 서로 다른 캐패시터형의 MONOS(metal-oxide-nitride-oxide-semiconductor) NVSM을 제작한 후, 경사전압 방법과 등전압 방법으로 절연파괴 특성을 측정하여 초박막 ONO 구조의 신뢰성을 조사하였다. 질화막이 23A˚인 초박막 ONO 유전막의 소자가 질화막이 두꺼운 소자에 비해서 우수한 신뢰성을 나타내었다. 따라서 초박막ONO 적층유전막은 MONOS/SONOS 구조를 갖는 비휘발성 반도체 기억소자의 고집적화를 가능하게 함을 알수 있다. Capacitor-trpe MONOS (metal-oxide-nitride-oxide-semiconductor) NVSMs with the same dimensions of the tunneling oxide(23A˚) and blocking oxide(40A˚), but different dimensions of nitride(45A˚. 91A˚ 223A˚) were fabricated. TDDB characteristics of MONOS devices were measured to investigate the reliability of ultrathin ONO structure using ramp voltage and constant voltage method. ultrathin ONO structure with 45A˚ nitride thickness was shown more good reliability than that with 91A˚ and 223A˚ Therefore. ultrathin ONO stacked dielectrics is Promising for scaling of nonvolatile semiconductor memory devices with SONOS/MONOS structure.

建物의 部位別 材料의 熱的性能에 關한 硏究 : 內斷熱 組積壁을 中心으로

金光瑞,梁克永,柳泳鎬 圓光大學校 1984 論文集 Vol.18 No.2

This Research is the basic study on the thermal performance of internal thermal insulating masonry structure, the experiment of the heat flow, for the efficiency at the spot of the insulating structure through the thermal copacty experiment. We gained that conclusion by comparing such results through the 12 external experiments.

벼 잡종초기세대에서 이삭줄기 대유관속 수와 이삭 특성의 분리양상

김광호,안종국,서경인 建國大學校附設農業資源開發硏究所 2001 農資源開發論集 Vol.23 No.-

The segregation modes and the selection efficiency of large vascular bundle(LBV) number and panicle characteristics were measured in three hybrid populations of rice. F_1 plants of the three crosses showed different direction and degree of dominance in vascular bundle number and panicle characteristics. The panicle neck diameter(ND), number of primary branches(PB) and LVB showed a typical normal distribution in F_2. When F_2 plants were divided into two groups up culm length of 90cm, the distribution of LVB number and panicle characteristics were not different between two populations. The selected and non-selected F_3 populations showed no difference in distribution of LVB number and panicle characteristics. However, F_3 populations grouped by culm length of 90cm showed different distributions in LVB and 100-grain weight among six characteristics observed. In F_2 populations, ND showed positive correlationship with PB, LVB number and grain number per panicle(GN). ND was positively correlated with PB, LVB number, GN and panicle length in selected and non-selected F_3 populations.

발육중인 생쥐 하악 과두에서 연골 및 골의 특이 유전자 발현

지국섭,윤영주,박주철,김광원 대한치과교정학회 2004 대한치과교정학회지 Vol.34 No.2

하악과두 연골이 발생하고 분화하는 과정에서 나타내는 특성을 규명하기 위하여, 발생 16, 18일과 출생 1일, 5일, 10일, 20일 및 30일 후의 ICR생쥐의 하악과두를 형태학적으로 분석하고, 생쥐 Ⅰ형, Ⅱ형, X형 교원질, Indian hedgehog(IHH) 및 BMP-4 등의 mRNA 발현을 in-situ hybridization 방법으로 연구하였다. 1. 생쥐 Ⅰ형 및 Ⅱ형 교원질 mRNA는 하악과두의 발생 및 성장과정에서 모두 발현되었다. Ⅰ형 교원질 mRNA는 휴지층과 증식층의 상부에서 관찰된 반면 Ⅱ형 교원질은 휴지층과 증식층 그리고 비대연골층의 상부에서 관찰되었다. 2. 하악과두 연골은 성장에 따라 비대연골층이 계속 증가하는 소견을 보였으며, 비대 연골층의 세포들은 특징적으로 X형 교원질 mRNA의 발현을 보였다. 3. BMP-4 mRNA는 하악과두 연골 원기와 골화중인 하악골체에서 모두 발현되었다. 4. IHH mRNA는 하악과두의 발생과정에서 증식 연골층의 하부와 비대연골층의 상부에서 선택적으로 관찰되었다. It has not been elucidated whether the initiation of condylar development of the mandible is related with the periosteum of the mandible, or if it derives from a separate programmed blastema not related with the mandible. Also, although the mandibular condylar cartilage is known to promote growth, few studies have dealt with molecular-biologic mechanisms such as the expression of specific genes according to the differentiation of the mandibular condyle. To elucidate the unique cellular characteristics, development, and differentiation process of the mandibular condyle, an examination of expressions of genes characteristic of cartilage and bone were carried out using RT-PCR and mRNA in situ hybridization. 1. Type?collagen mRNA was detected with type Ⅱ collagen mRNA in the differentiation and growth process of the cartilage of the mandibular condyle. Type Ⅱ collagen mRNA was demonstrated in the whole resting and upper part of the proliferative zone, whereas type Ⅱ collgen mRNA was observed in the resting, proliferative and upper hypertrophic cartilage zone of the mandibular condyle. 2. The condylar cartilage rapidly increased in size due to the accumulation of hypertrophic chondrocytes as characterized by the expression of type Ⅱ collagen mRNA during postnatal development. 3. BMP-4 mRNA was present in the anlage of the future condylar process and also in the ossifying mandibular body. 4. IHH mRNA was limited exclusively to the lower part of the proliferative zone and the upper part of the hypertrophic cartilage zone during condylar development. These findings were different from those in the growth-plate cartilage of the long bone, indicating a characteristic feature of the differentiation of the chondrocytes in the condylar cartilage present in prenatal and postnatal development. Furthermore, it was also suggested that chondroblasts of condylar cartilage rapidly differentiate into hypertrophic chondrocytes with increased functional Load force such as muscle activity and mastication.

建物의 部位別 材料의 熱的性能에 關한 硏究 : 外斷熱, 中斷熱 祖積壁을 中心으로

金光瑞,柳泳鎬,梁克永 圓光大學校 1985 論文集 Vol.19 No.2

The study is aimed at offering the basic materials of the effective insulatin supported system in the building construction place by estimating the thermal energy storage number and thermal performance and analyzing theoretical and experimental value in the insulating masonry wall structure. The results obtained were as follows 1. The experimental value of the thermal flow ratio in the inner and middle insulation wall structure is 5 ~ 10% higher than the theoretical value, while the experimental value in the experiment installed edge insulation is 3 ~ 7% lower than the theoretical value. 2. Both the theoretical and experimental value in the outer insulation is similar without the edge insulation construction. 3. If the thickness of each building material is same, "K" value of the wall structure is constant without the position of the insulation materials. But the temperature form in the structure is changeable according to the change of the insulation material position. 4. The experimental result of the thermal flow ratio like the theorectical function is in proporsition to the thermal transmission of the insulation materials. 5. The domestic insulation material capacity was confirmed by abtaining a desirable value from the thermal experiment of the outer wall installed by the domestic insulation materials, STYROPOR, and ISOPINK. In case of the middle insulation wall structure, the thermal resistance, 0.09㎡ h℃/kcal is obtained in the construction between the wall structure materials and insulation. 6. In case of the middle insulation wall, which is constructed only in korea, there is so large a difference of the thermal flow according to the outer wall's right edge, there is or not the edge-beam insulation of outer wall and construction sureness that extra insulation structure construction is necessary. When the cement masonry wall is installed by the insulation materials in indoor, outdoor and middle wall. The following results are gained through the analysis of the thermal performance. 1) In the outer and middle insulation wall structure, according to the increase of the insulation material thickness, the energy storage storage number is increasing but decreasing oppositely in the inner insulation wall structure. The erergy storage number depends on the position of the insulation materials. 2) The increase of the vapor expansion resistance ratio is in proporsition to the thickness of the insulation materials without the installation position of the insulation materials. 3) The relation of phase, the scale of which is the largest in the outer insulation, second in the inner insulation, third in the middle insulation, is in proporsition to the thickness of the insulation materials. 4) According to the outer air temperaure, the change of the indoor surface temperature reaches 20℃ by increasing the thickness of the insulation materials that it makes the indoor environment comfortale in the thermal point.

建築材料의 熱傳導特性에 關한 硏究 : 콘크리트 熱傳導率을 中心으로 Laying Stress on the thermal conductivity concrete

梁克永,金光瑞 圓光大學校 1984 論文集 Vol.18 No.2

This object aims to study the influence of different kind of building raw materials of thermal conductivities upon concrete by experiment and came to the conclusion as follows. 1. When the unit quantity of cement and fine aggregate showed the rate of increase the thermal conductivity is appeared to be higher figure. 2. Even in the unit weight of cement, fine aggregate ratio and water cement ratio are equal, the figure of the thermal conductivities of different kinds of aggregates. because the materials such as slag, of which the surface are rough, have strong adhesive power of aggregate and mortar, is relatively appeared to be high. But on the other hand as the materials such as texes, of which the surface are smooth have weak adhesive force, the figure of the thermal conductivity is appeared to be low.

강우시 채수빈도가 논 오염부하량 산정에 미치는 영향

한국헌,김진호,이종식,이정택,조재영,윤광식 한국환경농학회 2005 한국환경농학회지 Vol.24 No.1

한정된 수질자료를 이용 오염부하 산정시 집중적으로 많은 샘플을 채취한 경우에 비해 어느 정도 차이를 갖는지를 규명하는 것은 모니터링에 따른 오염부하량 산정결과의 신뢰 범위 파악뿐만 아니라 경제적인 수질 샘플 채수빈도 수립에 곡 필요한 것으로 사료된다. 그 결과를 요약하면 다음과 같다. 논에서의 강우-유출시 채수 빈도가 오염부하 산정에 미치는 영향을 파악하기 위해 9개의 강우사상에 대해 2시간 간격으로 연속 수질샘플을 실시하여 분석한 결과 전체적으로 강우-유출과정 중 T-N, T-P, SS 농도는 시간별로 증·감변동을 하였으나 유량과의 상관성은 나타나지 않았다. 수질샘플 횟수에 의한 영향은 강우-유출과정 중 5회 정도 샘플을 실시하면 많은 수의 샘플을 채수한 경우에 비해 T-N ±15.2%, T-P는 -20.0~26.2%, SS는 -28.6~35.7% 범위안에서 오염부하를 추정할 수 있는 것으로 나타났으며, 차이범위가 ±10% 내에 들어가기 위한 샘플수는 일주기 조사시 T-N, T-P의 경우는 6회, SS의 경우는 단기유출시는 4회 정도 실시하면 되고, 장기 유출시에는 11회 정도 실시하면 되는 것으로 조사되었다. 유량-유하부하량 관계식으로 ±10%내의 차이를 보일 샘플횟수는 T-N의 경우는 6회 이상, T-P와 SS는 9회 이상 정도 실시하면 되는 것으로 조사되었다. In order to examine effects of sampling frequency during rainfall-runoff process from paddy field on the estimation of pollution load, EMCs of several water sampling frequencies were examined. Water quality samples were conducted by every two hours interval for each event. It was found that difference of load estimation between five times sampling and two hours consecutive sampling during rainfall-runoff showed 15.2~-15.2% for T-N, 20.0~-26.2% for T-P, 28.6~-35.7% for the SS, respectively. In the same way, the effects of number of sampling data on estimation of pollution load using runoff-mass load(L-Q) method were investigated. L-Q equation made of five times sampling data provided 10% differences in estimation of mass loads of T-N, T-P, and SS when compared to those by L-Q equation using entire two hours consecutive sampling data during runoff process.

有限要素法에 依한 Notch部의 應力解析

崔國光 慶北工業專門大學 1974 論文集 Vol.11 No.-

The stress concentration factors of U-notched and V-notched plate subjected to tensile force are calculated by finite element method. Photo-elastic experiment are also carried out. The solutions are compared with Neuber's and Inglis and Armbruster's formula. The following properties are investigated; (1) The data of photo-elastic experiment of U-notched plate are 0.5% greater than the calculated data of finite element method. (2) The data of photo-elastic experiment of V-notched plate are 0.4% greater than the calculated data of finite element method. (3) The calculated data of Armbruster's formula are 1.7% greater than those of U-notched plate by finite element method. (4) The calculated data of Inglis formula are 10% greater than those of V-notched plate by finite element method. The data of photo-elastic experiment among three analysis methods agree satisfactorily with the calculated data by finite element method. This paper is shown that analysis of notched plate by finite element method obtain accurate-solution.