아스코르빈산의 식품생화학적 성질에 관한 연구 : 소맥단백질에 대한 아스코르빈산의 작용

변광의 순천향대학교 1986 논문집 Vol.9 No.1

제빵발효중 첨가된 L-Ascorbic Acid로 인한 소맥단백질의 이화학적 성질의 변화를 관찰하기 위하여 소맥품종으로 Carimini'76과 Schirokko'79를 사용하였다. 각 시료의 단백질 성분을 2-Chloroethanol, 그리고 H₂O, 2-Chloroethanol 및 70% Acetic acid로 연속 추출분리하였으며, Starch gel electrophoresis, SDS-PAGE, Isoelectric Focusing 및 Thin-layer Gelfiltration(TLG)를 수행하여 얻은 결과를 요약하면 다음과 같다. (1) Carimini'76으로부터 2-Chloroethanol-Soluble 단백질의 Starch gel 전기영동 pattern에서는 Gliadin 및 Album fraction에서 Polypeptide의 intensity증가와 새로운 band의 형성을 보였다. (2) SDS-PAGE에 의한 분자량수준에서의 변화로서는, Carimini'76의 경우 4개의 Polypeptide Chain에 L-Ascorbic Acid의 작용효과가 나타났으며, 그들의 분자량 범위는 1.2×10⁴∼1.5×10⁴Dalton(Low-Molecularweight-Gliadin)과 5×10⁴∼6×10⁴ D(High-Molecularweight-Gliadin)으로 확인되었다. (3) Schirokko'79에서는 Water-Soluble단백질의 PI가 L-Ascorbic Acid를 작용시켰을 때 more basic pH쪽으로 이동함을 보였으며, 2-Chloroethanol-Souble Gliadin에서 5×10⁴∼7×10⁴D의 Polypeptide들이 감소하면서 1.5×10⁴∼2.5×10⁴D영역에 새로운 Polypeptide band가 출현하였다. (4) 두 품종으로부터 얻은 Gliadin단백질을 Sephadex G-100으로 Gelfiltration한 바, 3개의 Subfraction으로 분리되었으며 relative Retention-Value로 비교했을 때 분자량의 증가현상을 보였다. 이와 같은 현상들을 종합하여 볼 때, L-Ascorbic Acid는 현재까지 알려진 바와 같이 산화형인 dehydro-form으로 Redoxprocess에 참여한다기 보다는 L-As-corbic Acid 자체 또는 그 분해산물들이 저분자 또는 고분자의 Gliadin성분과 Addition 또는 Substitution 반응을 통해 derivatives를 생성하는 것으로 사료된다. In order to observe changes for physico-chemical properties of wheat flour proteins owing to the added L-Ascorbic Acid in bread-fermentation, Carimini´76 and Schirokko´79 cultivars were examined. The wheat flour proteins were extracted with 2-Chloroethanol from Carimini´76, on the other hand they were stepwise extracted from Schirokko´79 with water and then 2-Chlooethanol and 70% Acetic in the order. These proteins were examined by starch gel electrophoresis, SDS-PAGE, Isoelectric Focusing and Thin-Layer Gelfiltration. The results were as follows; 1. At the starch gel electrophoretic patterns from Carimini´76, an increment of polypeptide intensity and the formation of new band for Gliadin-and Albumimfraction from 2-Chloroethanol soluble proteins were appeared. 2. Investigation of Carimini´76 by SDS-PAGE for the molecularweight determination was proved to be changed in 4 polypeptide chains through the action of L-Ascorbic Acid. It was recogniged that the molecularweights of those covered the range of 1.2×10⁴-1.5×10⁴ dalton. 3. pI values of water soluble proteins from Schirokko´79 were moved to more basic pH ranger after the treatment of L-Ascorbic Acid. Gladins soluble in 2-chloroethanol showed the decrease in molecularweight in the range of 5×10⁴-7×10⁴D, while the new polypeptide band emerged in the region of 1.5×10⁴-2.5×10⁴D. 4. Gladins from the two cultivars were separated into three polypeptide groups by Sephadex G-100 TLG and appeared to be increased in molecularweights estimated by the relative retention-values. In results, it was believed that the L-Ascorbic Acid do not participate in the redoxprocess as an oxidized dehydro-form, but L-Ascorbic Acid or its decomposition-products produce a derivatives through the reaction of addition or substitution with lower or higher molecular Gladian components.

식용색소 생산을 위한 생물반응기의 모델 제작 및 운용 연구

변광의 순천향대학교 기초과학연구소 1999 순천향자연과학연구 논문집 Vol.5 No.1

The solid-state-fermentation has been focused as a very useful traditional tool in biotechnology. The Monascus Red-rice, Ang-kak, was produced by SSF model-bioreactor and has found an increased interest in the past few years. The model-bioreactor, constructed with new type impeller system, has been operated under the controlled conditions and the fermented product was analyzed daily. The substrate rice, treated with K-300, has shown the improved color intensity and reduced agglomeration remarkably in bioreactor. The changes in pigment intensity and water activity were checked regularly for 10 days and proved to be formed normally. More efforts to revise bioreactor automatically would be paid in order to develop Pilot-or Industrial- Bioreactor.

제2형 당뇨병 환자에서의 지속적 피하 인슐린 주입 치료시 관해

최의광,최수봉,박선민 건국대학교 의과학연구소 2001 건국의과학학술지 Vol.11 No.-

Background: It is well known that chronic hyperglycemia can deteriorate pancreatic beta cell function and insulin sensitivity in animal and human studies, and the normalization of blood glucose can reverse them. Our purpose of the study was to investigate the remission pattern after treating patients with continuous subcutaneous insulin infusion (CSII) therapy, and compare the clinical characteristics of remitted and non-remitted patients in a retrospective way. Methods: We selected 91 type 2 diabetic patients who had been admitted to KonKuk University hospital from January 1993 to August 1993. After hospitalization, all subjects were treated with insulin pump (Sooil, Seoul, Korea) for intensive blood glucose control until remission occurred. Remission is defined as long-term good glycemic control without any medication. After fifteen to seventeen months of CSII therapy, the induction of remission was examined. Serum c-peptide levels, hemoglobin A1c, and serum lipid profiles were measured after hospitalization and before discharge. Results: Overall 34.4% of all subjects had remission after 53.6±38.9 days of CSII therapy and remission lasted for average 13.6 ± 8.9 months during the study periods. Some clinical characteristics of the patients prior to CSII therapy influenced the remission occurrence. The remission was more frequently induced when patients started CSII therapy with insulin pump in younger age, higher body mass index, shorter diabetic duration, lower post-prandial blood glucose levels, higher post-pandial serum c-peptide levels, and less chronic diabetic complications. Conculsion: These findings suggest that long-term CSII therapy can induce remission in a significant proportion of mild type 2 diabetic patients. Thus, it is desirable that the intensive insulin treatment by CSII is considered as not the last treatment., but an initial management of mild type 2 diabetic patients.

肝組織에서의 Glucagon의 脂肪酸合成 抑制機轉에 대한 硏究

曺義鉉,高光三 조선의대 1984 The Medical Journal of Chosun University Vol.9 No.1

제빵발효중 동위원소로 표식된 아스코르빈산의 행방에 관한 연구

변광의 순천향대학교 1991 논문집 Vol.14 No.3

제빵반죽의 발효중에 첨가되는 L-Ascorbic acid의 소맥성분에 대한 작용 Mechanism 을 연구하는데 있어서 문제점은 L-Ascorbic acid자체의 불안정성으로 인한 반응과정 추적이 어려운 점에 기인된다고 본다. 본 연구에서는 ??-L-Ascorbic acid를 병용하고 그 행방을 추적하였으며 얻어진 결과는 다음과 같다. 1. 제빵반죽시료는 L-Ascorbic acid와 1-??-L-Ascorbic acid 혹은 U-??-L-Ascorbic acid(total 20PPM)를 첨가하여 반응후, 급속동결 및 동결건조 시켰고, 여러가지 용매를 이용하여 추출한후, 다시 동결건조시킨 시료를 gel chromatography로 분리 연구하였다. 2. 제빵반죽시료로 부터 약 80% 이상의 radioactivity가 추출 회수 되었으며, 그중 3/4은 저분자분획에서 발견되는데 반하여, 약 1/4은 분자량이 1,000 dalton 이상인 고분자분획에서 발견되었다. 또 urea 추출물 중에는 전체 radioactivity의 약 5%가 투석막을 통과하지 못했다. 3. Sephadex LH-20 및 G-25를 이용한 gel chromatography 에서는 2개의 band로 분리되었으며, Turbidity, radioactivity 및 protein 성분들이 확인된 바, 고분자 fraction l은 소량의 radioactivity를 함유하는 반면,단백질분획중 fraction Ⅱ/a 는 다량의 radioactivity를 동반하였다. 동시에 fraction Ⅱ는 L-Ascorbic acid를 함유하고 있음이 확인되었다. 4. DEAE-Cellulose(borate형)에 의한 분리에서는 전체 radioactivity가 3개의 fraction으로 분리되었으며(fraction Ⅰ,Ⅱ,Ⅲ), 그 중fraction Ⅱ는 다시 3개의 subfractions(Ⅱ/a, Ⅱ/b, Ⅱ/c)로 세분되었다. fraction Ⅰ과 Ⅱ/a는 현저한 량의 radioactivity와 browning reaction products 외에도 다량의 단백질을 함유하였다. 또 L-Ascorbic acid는 fraction Ⅲ에서 발견되었다. fraction Ⅰ이 480nm에서 강력한 흡수대를 나타내는 것으로 보아 fraction Ⅰ,Ⅱ/a, 및 Ⅱ/c에는 pep-tides 또는 protein들이 L-Ascorbic acid에 의해 modified derivatives로 변화됨을 시사한다. 5. Sephadex G-100에 의한 thin-layer gelchromatography에서 2-chloroethanol에 가용성인 gliadine 단백질은 3개의 polypeptide fraction(fraction 3', 4', 5')과 5개의 radioactivity fraction(1, 2, 3, 4, and 5)을 함유하고 있으며, 이들 polypeptide fraction들은 분자량이 각각 1.25 x 10⁴D., 2.50 x 10⁴D. 및 6.00 x 10⁴D.정도였으며, 전체 radioactivity 총량은 solvent extraction에서 ultrafiltration후의 고분자 fraction의 radioactivity 총량과 근사치를 보였다. 전체 결과를 요약해 볼 때 ??-L-Ascorbic acid 및 그 분해산물의 행방으로 보아 L-Ascorbic acid는 소맥내의 redox-process에 관여하기 보다는 소맥의 저분자 또는 고분자성분 특히 protein 분자와 직접 또는 분해산물 형태로 반응하여 새로운 결합형태를 유도하며, 특히 물성의 변화와 밀접한 망상구조에 변화를 초래하는 것으로 사료된다. In order to study the reaction mechanism of L-ascorbic acid added during wheat bread dough fermentation, we have prepared the dough treated with ??-labeled L-ascorbic acid and investigated the radioactivity through stepwise solvent extraction. About 80-90% of the total radioactivity were recovered from ??-dough extracts and the 1/4 of them were found in high molecular fraction after ultrafiltration, while the 3/4 in low molecular fraction. In Sephadex LH-20 and G-25 gel chromatographic separation, 60% EtOH or H₂O extracts revealed 2 fractions and each eluate was examined on turbidity, radioactivity and protein components. The fraction Ⅱ/a contained the major quantity of radioactivity, while the fraction l the minor. In DEAE-cellulose chromatographic separation, the total radioactivity were separated into 3 fractions and then the fraction Ⅱ was divided again into 3 subfractions(Ⅱ/a, Ⅱ/b, Ⅱ/c). The fraction Ⅰ and Ⅱ/a among them have contained a significant quantity of protein components as well as radioactivity and browning reaction products. The L-ascorbic acid has been found in fraction Ⅲ. The peptides and proteins in fraction Ⅰ, Ⅱ/a and Ⅱ/c could be modified or derivated by the L- ascorbic acid action. In thin-layer gel chromatographic separation by Sephadex G-100, the gliadine protein was separated into 3 fractions (3',4',5')and revealed 5 radioactivity fractions(1,2,3,4,5). The molecular weight of each of these fractions are about 1.25 x 10⁴ D ,2.50 x 10⁴D, and 6.00 x 10⁴D and the radioactivity 77.23%, 3.21%, and 5.09%, respectively. The total amount of radioactivity of higher molecular polypeptide fraction 4' and 5' was similar to that of high molecular fraction ultrafiltered from stepwise solvent extracts. Consequently, the behavior of the ??-L-ascorbic acid led us to the suggestion that in the redox-processes, the L-ascorbic acid does not simply react with wheat flour, but rather reacts with low molecules or high molecular components, especially protein molecules. It was also possible that the decomposition product of the L-ascorbic acid induces the another type of reactions. This fact implies changes of protein-network closely related to the rheological changes of dough.

내츄럴 스낵제품의 공정개선 연구

변광의,이진경 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.2

This subject was intended to determine the optimal concentration of isomalto-oligosugar treated to fruits snack, which was produced by the vacuum-frying techonology and estimated by rheological and sensoric quality test. We have mainly investigated the impact of oligosugar concentration added and the soaking frequency on the quality of vacuum fried snack-products using HPLC system. As a result, it was found that higher concentration of oligosugars likely led to higher desirability in both apple and persimmon snack, and 25 Brix was best preferred in both snacks. Isomalto-oligosugar at 25 Brix as a solution for soaking in this study was composed of 4 elements. As a result of analysis, it was shown that major sugar and organic acids of apple snack before soaking were sucrose, glucose, fructosse and malic acid, and major sugars of persimmon snack were sucrose, glucose and fructose. For the solid of apple and persimmon snack soaked in isomalto-oligosugar, it was found that two snacks absorbed more or less isomalto-oligosugar under the influence of osomotic pressure, resulting in the formulation of total 5 peaks, which obviously indicates the effects of oligosugar permeability.

解放 後 左右合作運動의 展開過程에 관한 硏究

兪光震,黃儀敍 東國大學校 1995 東國論叢 Vol.34 No.-

After Liberation of the Korean Peninsula, August 15, 1945, the United States and the Soviet Union Armed Forces were stationed on the North and the South Korea being the boundary line of the 38th Parallel. For the solution of this Korean problems, the United States and Soviet Union offered proposals of Trusteeship. Because the failure of the agreement between the U.S. and the Soviet Union in trusteeship consensus, the 38th parallel was constructed the real wall. As the greatest matter of the Korean people the time was the building of the Unified Nation States, that matters required the international cooperation, reopening the Joint Commission. They required the wide gathering of the inter-nation political leaders and groups so that the Joint Commission reopened, this mission was operated the Right and Left wing coalition Movement groups. The purpose of the Coalition Movement was the the building of the Unified Nation States in Korea, through the consensus of the international political groups and leaders. This Study focuses on the process of the Coalition Movement after liberation in Korea. The process of the Coalition Movement was, the first step(1946.5-1946.10): Starting of the Coalition Movement and consensus of the coalition principles, the second step(1946.10-1946.12):Opening of Korea-U.S. Common Conference(the agenda-the pending bills of the time) and establishment of the Interim Legislative Assembly of the south Korea under the USAMGIK, the third step(1947.1-1947. 12: Reopening and rupture of the second Joint Commission and disassembling of the Coalition Committee. The Coalition Movement ended in failure on account of the negative attitudes and the disturbances of the extreme rights and the extreme lefts, but the Coalition Movement is still the historical manifestation in that the Korean Peninsula has continued the 38th parallel during 50 years, therefore this movement get a lot of suggestions in the Reunification of Korea.

정보 시스템을 이용한 항공기 착륙요율 결정 사례 연구 : 잔액 보상 방식에 의한 착륙요율 결정 방법 중심

유광의,김봉균 한국항공운항학회 1998 한국항공운항학회지 Vol.6 No.1

The purpose of this research is to look for the best description of calculating the reasonable Landing Fee. Landing Fee is consisted one of major revenues for maintaining an airport. Traditional Landing Fee Rate has been charged based on the weight factor; Maximum take-off weight, Maximum landing weight, or Maximum authorized weight. To achieve a better reliable value of Landing Fee Rate, The elements of Noise and Peak-Time have to be considered as well as the aircraft weight. This research designs the algorithms for calculating Landing Fee Rate and also Landing Fee, based on the aircraft weight. The Network is also applied to above. That is, CGI(Common Gate Interface) is constructed to interface the terminal of calculating Landing Fee Rate, and the terminal of collecting and transmitting the data such as the Weight. The computer language on the CGl was made by C++ and PERL. The main point of this research is to integrate the airport and Information System and to construct the database which is based on the different perspective of calculating Landing Fee Rate. However, the result of the most efficient and reliable will be computed based on above. This research will broaden the range of application up to the each case of airports.

Monascus purpureus를 이용한 쌀로 부터 천연식용 색소생산에 관한 연구

변광의,장성근,고영주 순천향대학교 기초과학연구소 1995 순천향자연과학연구 논문집 Vol.1 No.1

Studies on the optimal conditions for the production of natural edible red pigments with Monascus purpureus Went were performed. The optimal pH of strain on agar medium was 5.5 and the optimum autoclaving time for rice medium was twenty minutes. Analysis of food components of the raw or the fermented rice showed a remarkable changes: the contents of components were increased in moisture, crude protein and crude fat after the fermentation, but decreased in starch. starch. There was no difference in ash content but the reducing sugar in the fermented rice was detectable. The color intensity of the fermented rice revealed a wide range of differences among the regions produced. The water activity of rice medium was decreased but colony counts were increased during the fermentation periods. In the ergosterol assay, the peaks of ergosterol standard and fermented rice revealed a similar retention time. Changes in food components during the fermentation periods were daily investigated: the contents in moisture, crude protein and reducing sugar were increased but in starch content decreased. The water soluble extracellular pigment and the alcohol soluble intracellular pigment were increased continuously but the water soluble total pigment decreased at 10 day. In effect of inorganic salts, (NH₄)₂SO₄. FeSO₄·7H₂O. NH₄NO₃. MnO₂. NaN₃ and KNO₃have stimulated the pigment production. The absorption spectra of pigment solutions exhibited the maximum at 400nm and 500nm On thin layer chromatogram, the red pigment preparation revealed five fractions {R₁(??) -R₄} and yellow pigment preparation consisted of single fraction(Y₁). These basic results will be applied for studies of the polyketide biosynthesis pathway, the innovation of solid-state bioreactor and the production of natural food ingredients.

패러글라이딩 전방이륙시 라이즈-업 동작의 키네매틱 분석

김의환,조효구,백광현 한국운동역학회 2000 한국운동역학회지 Vol.9 No.2

본 연구는 항공스포츠 중 몇 년 사이에 급속도로 발전한 패러글라이딩(paragliding)의 전방이륙(take off)기술 중 라이즈-업(rise-up) 동작에 관한 키네매틱 변인의 기초자료를 얻는데 그 목적이 있다. 연구의 목적을 달성하기 위하여 대한패러글라이딩협회에 등록된 Y.대학 남자선수 10명을 대상으로 라이즈-업 동작을 각 5회씩 실시하여 연구자가 그 중에서 가장 잘된 동작 1회를 비디오 테입에 편집하여 국내 패러글라이딩 전문가에게 평가를 의뢰하여 상위 5명을 선정한 후 키네매틱 변인을 분석하였으며 결과는 다음과 같다. 시간변인에 있어서 제 1국면이 전체 소요시간 중 57.45%(1.53sec)가 걸렸으며 2국면의 소요시간이 길수록 전체시간이 증가하는 것으로 나타났다. 거리변인에서는 제 2,3국면보다 제 1국면에서의 소요거리(2.25±0.36cm)가 51.49%로 가장 길게 나타났다. 각도변인에 있어서 팔꿈치각은 어깨각과 함꼐 상호 보상작용을 통한 캐노피의 균형을 유지하는 역할을 수행하는 것으로 나타났으며, 발목각은 피험자 개개인의 신장과 보폭의 차이에서 발생하는 편차가 종목 특성으로 큰 차이를 보였다. 또한, 상체 전경각에 있어서 저항이 큰 Cg이벤트에서 굴곡이 이루어졌다가 Hp이벤트에 와서는 신전 시킨 후 캐노피와의 견제를 위한 굴곡이 다시 이루어진 것으로 나타났다. 속도변인으로는 신체중심의 속도변화에서 출발신호와 함께 진행방향의 역방향으로 -0.02m/sec의 탄력적인 신체중심의 준비형태를 보였다. The purpose of this thesis is to investigate the basic Data Bases which were based on the rise up motion through kinematic variables analysis of rise up motion among the take off technique of the paragliding. To understand this thesis I made use of three dimensions cinematography through DLT method and also analyzed the kinematic variables through velocity variables, distant variables, angle variables and temporal variables. Therefore the conclusion were as follow The first phase is time required of use 57.46%(1.53sec) the total of the time, and second phase of the time longer than increase of the total time. For the first phase distance required of use 51.49%(2.25±0.36m) longer than second and third phase. In case of the valicity change of center of gravity of each events was showed toward -0.02m/sec.