Effects of Caponization on Bone Characteristics and Histological Structure in Chickens

Kuo-Lung Chen,Ming-Huang Chang,Shiow-Min Tsay,Huaang-Youh Hurng,Peter Wen-Shyg Chiou 아세아·태평양축산학회 2006 Animal Bioscience Vol.19 No.2

The aim of this study was to investigate the effects of caponization on the bone characteristics, biomechanical property and histology in Taiwan country chickens fed to market age of 26 wks. Male Taiwan country chickens D횞L2 were caponized or shamoperated at 8 wks of age, and selected healthy sham-operated and completely caponized chickens (prominent degenerated comb) were selected at 16 wks old and fed to 26 wks old for the trials. Fifteen intact male chickens (Intact), sham-operated chickens (Sham) and caponized chickens (Capon) were assigned for trial 1, and sixteen Intact and Capon were assigned for trial 2. Results in trial 1 showed that the abdominal fat and relative abdominal fat weights of Capon were significantly heavier than Intact and Sham (p<0.05), while the tibia weight and relative weight were the lightest (p<0.05). The tibia breaking strength, bending moment and stress of Capon were the poorest among groups (p<0.05). The trial 2 produced the similar observation that Capon were significantly lighter than Intact (p<0.05) in the tibia weight, relative tibia weight and their biomechanical properties. On histological determinations, Capon showed a thinner cartilage end and fewer chondrocytes (about 50%) and trabecular, and bigger marrow cavity; while decreased hemopoietic cells number with increased adipocytes than Intact observed by H&E stain and at low magnification. At high magnification, Capon showed a decrease in the chondrocyte size by 33 to 50%, with smaller nucleus located near the cell membrane, and exhibited monocellular form chondrocytes. Capon also showed a less strongly acidic sulfated mucosubstance with weaker dyeing property within cartilage zone, and smaller chondrocytes size by Alcian blue stain.

Effect of Dietary Inclusion of Dehydrated Food Waste Products on Taiwan Native Chicken (Taishi No. 13)

Chen, Kuo-Lung,Chang, Hwang-Jen,Yang, Ching-Ke,You, Shanq-Huei,Jenq, Horng-Der,Yu, Bi Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5

The effect of dehydrated food waste product (DFWP) inclusion in diets of Taiwan native chickens on the growth performance, carcass traits, clinical blood chemistry and nutrient digestibility was investigated with 320 male Taishi Native Chickens (Taishi No. 13). They were randomly allocated into four levels of DFWP inclusion (0, 5, 10, or 20%) treatment. Each treatment had 80 chickens with four replicates. At 15 weeks of age, 8 chickens were selected from each group for the nutrient digestibility trial. The results showed that body weight gain during the 0 to 4 week period decreased as the feed intake and feed conversion rate (FCR) increased linearly with increasing level of DFWP inclusion. During the 4 to 8 week period, greater body weight gains were recorded for the birds on the 5% DFWP inclusion level than for the control (p<0.05). During 8 to16 weeks, the feed intake and FCR increased linearly (p<0.05). Interestingly too, results of the carcass analysis showed that DFWP inclusion up to 20% in the diet significantly decreased the relative abdominal fat weight but increased the relative proventriculus and gizzard weights of the birds. However, other carcass parameters, meat quality and sensory scores were not significantly influenced by the dietary DFWP inclusion levels (p>0.05). Higher serum aspartate aminotransferase, ${\gamma}$-glutamyltransferase activity and crude protein digestibility, and lower gross energy digestibility were observed in the 20% DFWP group compared to the control (p<0.05). Dietary DFWP inclusion was inappropriate during the 0 to 4 week period. During 4 to 8 weeks, there was no adverse effect on chicken performance in the 20% group. There was no disadvantage on carcass properties and in the meat panel test. This result would not affect consumer determination.

A Statistical Framework for Characterizing the Nanotube Buckypaper Manufacturing Process

Chi-Lung Kuo,Chih-Hsing Chu,Che-Ping Jack Su,Hsin-Yuan Miao 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

This study develops a statistical framework for characterizing an improved Buckypaper manufacturing process. A screening procedurebased on design of experiment techniques and the Lenth method was used to identify significant process parameters. These parameterssignificantly affect the tensile strength of the Buckypaper produced by the process. The procedure only samples a limited number ofexperimental runs. A linear regression model and a nonlinear model based on the Kriging method were constructed to characterizethe relationship between the parameters and tensile strength. Tradeoff curves were used to determine the best parameter settings whensimultaneously considering the mean and variance of the tensile strength. Validation tests show that both models are better predictorsthan previous models and the nonlinear model outperforms the linear model. The statistical framework provides a systematic andeffective method of characterizing the manufacture of nanotube products.

The Effects of Caponization Age on Muscle Characteristics in Male Chicken

Chen, Kuo-Lung,Chen, Tsai-Tzu,Lin, Kou-Joong,Chiou, Peter Wen-Shyg Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.11

This study examined the caponization effects on the muscle characteristics (quality and quantity) of caponized male chickens fed before or after sexual maturity. Healthy and uniform Single Comb White Leghorn chickens were caponized at 3-week-old. Feeding was conducted until 16-week-old in trial 1 or from 12-week-old to 26-week-old in trial 2. Ten sham operated male chickens (Sham) were also assigned to each trial as the control group. Chickens used in both trials were housed in individual cages with each chicken representing one replicate. The results showed that early caponization (3-week-old) significantly increased (p<0.05) body weight and pectoral major muscle weight and percentage at 16-week-old compared to the Sham in trial 1. Caponization significantly increased (p<0.05) the protein content of the pectoral major muscle, but decreased (p<0.05) the ash content. Late caponization (12-week-old) significantly decreased (p<0.05) the ash content, myofibrillar ATPase activity and emulsification capacity of the pectoral major muscle in mature capons (26-week-old) compared to the Sham in trial 2. Early caponization (3-week-old) only increased the weight and protein content of the pectoral major muscle with decreased ash content in 16-week-old capons. Late caponization (12-week-old) showed no affects on pectoral major muscle quantity, while it decreased the ATPase activity and enhanced the emulsification capacity in mature (26-week-old) capons. Hence, the muscle quality improvement was shown as capons were fed to sexual maturity.

Retiform Hemangioendothelioma of the Neck

Chin-Lung Kuo,Pen-Yuan Chu,Paul Chih-Hsueh Chen,Wing-Yin Li 대한병리학회 2015 Journal of Pathology and Translational Medicine Vol.49 No.2

Effects of Caponization and Testosterone on Bone and Blood Parameters of SCWL Male Chickens

Chen, Kuo-Lung,Tsay, Shiow-Min,Lo, Dan-Yuan,Kuo, Feng-Jui,Wang, Jiann-Hsiung,Chiou, Peter Wen-Shyg Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5

This study was to investigate the caponization effects on bone characteristics in male chickens, and the optimum testosterone implantation dosage on bone characteristics improvement. Healthy Single Comb White Leghorn cockerels were caponized at 12-wk-old and selected at 16-wk-old for a 10-wk feeding experiment. Fifteen intact male and caponized male chickens (capon) respectively were assigned to trial 1. Ten sham-operated chickens and 40 capons (randomly allocated into four treatments) were implanted with cholesterol (1.62 mm i.d., 3.16 mm o.d., $9.24{\pm}0.36$ mg), low (1 mm i.d., 3 mm o.d., $5.88{\pm}0.23$ mg), medium (1.62 mm i.d., 3.16 mm o.d., $9.81{\pm}0.17$ mg) or high dose (2 mm i.d., 4 mm o.d., $16.7{\pm}0.24$ mg) of testosterone in trial 2. The results from trial 1 showed that the tibia length, relative tibia weight, breaking strength, bending moment and stress in intact males were higher than capons (p<0.05). The blood phosphorus concentration in capons was higher than the intact male chickens (p<0.05). Caponization also resulted in more antrums and osteoclasts within periosteum and cortical bone from histological observation. In trial 2, the adverse impact of caponization on the bone breaking strength, bending moment and stress could be alleviated through medium dose testosterone implantation. It appears that caponization reduced androgen secretion hence influenced the biomechanical characteristics of bone (tibia) and these adverse effects could be alleviated through appropriate dose of testosterone implantation.

Effects of Dietary Arsenical Inclusion on Lipid Metabolism and Liver Function in Mule Ducks

Chen, Kuo-Lung,Chiou, Peter W.S. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.3

This study evaluated the effectiveness of different arsenical sources on inducing fatty liver, on changes in lipid metabolism and on liver function in mule ducks. Sixty twelve-week-old mule ducks were selected and randomly divided into five treatments, including the control group and four different arsenical sources; Roxarsone (300 mg/kg), arsanilic acid, $As_2O_5$ or $As_2O_3$, containing 85.2 mg/kg arsenic were included in the basal diet. The ducks were fed the medicated basal diet for 3 weeks followed by a one-week drug withdrawal. The results showed Roxarsone treatment decreased body weight, feed intake, liver weight and abdominal fat weight (p<0.05), while it increased the relative liver weight (p<0.05) during medication period ($3^{rd}$ week). The $As_2O_5$ treatment decreased abdominal fat weight and relative abdominal fat weight when compared to the control (p<0.05). Only Roxarsone among the treatment groups increased feed intake, liver weight and relative liver weight, while the $As_2O_3$ group showed the lightest liver weight and relative liver weight among treatment groups during the withdrawal period ($4^{th}$ week). The Roxarsone group decreased (p<0.05) NADP-malic dehydrogenase (MDH) and acetyl-CoA carboxylase (ACC) activities and increased (p<0.05) cholesterol concentration during the medication period, and elevated the MDH and ACC activities during the withdrawal period. All four arsenical treatment groups showed lymphocytic infiltration in liver tissue, while the Roxarsone and $As_2O_3$ treatments showed an increase in aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) activities (p<0.05). During the withdrawal period, arsenical treatments resulted in liver vacuoles. However, the arsenicals differed in effectiveness and mechanisms of inducing fat vacuoles.

Caponization Effects on Growth Performance and Lipid Metabolism in Taiwan Country Chicken Cockerels

Chen, Kuo-Lung,Hsieh, Tseng-Yueh,Chiou, Peter Wen-Shyg Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.3

This trial was designed to study the caponization effects on the appearance, carcass characteristics, blood constituents and lipid metabolism of Taiwan country chicken cockerels. Cockerels were caponized at 8 weeks of age. Sixteen-week-old chickens, including 10 capons, 5 slips (incomplete caponized male chickens) and 20 normal chickens of equal sexes were selected for a 10 week ad libitum feeding trial. Results showed that the testosterone concentrations in the capons and females were lower (p<0.05) than that of intact males. The comb length, height and weights were also lower (p<0.05). The weight of the slips was between that of the capons and intact males, but was heavier (p<0.05) than that of the capon. The live-weight, carcass weight and shank perimeter in the capons were higher than those of the other groups (p<0.05). Hepatic lipogenic enzyme activity analyses showed that NADP-malic dehydrogenase (MDH) activity in the capons and female chickens was higher than that in intact male chickens (p<0.05). The MDH activity in the slips was between that for the capon and intact male chickens (p>0.05). The abdominal fat weight and relative abdominal fat weight of the capons and females were heavier than that for intact males (p<0.05); the slips were between the capons and intact males. The blood lipid content results showed that the triacylglycerol and cholesterol in the capons were higher than that for intact males (p<0.05). However, the percentage of low-density lipoprotein (LDL) was lower than that in intact male chickens (p<0.05). It appears that the increase in lipid accumulation in caponized male chickens is attributed mainly to an increase in MDH activity and the changes in lipid transportation in the capons.

Effect of Restrict Feeding, Roxarsone or Its Analogues in Inducing Fatty Livers in Mule Ducks

Chen, Kuo Lung,Chiou, Peter W.S. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.2

This study is aimed at understanding the role of arsenic in Roxarsone in causing fatty livers in mule ducks. One hundred 10-week-old mule ducks were randomly divided into 5 groups. Ducks received 2 weeks of various treatments followed by 2 weeks of withdrawal. The treatments were non-treatment (control), 300 mg/kg Roxarsone inclusion for 2 weeks ($1^{st}$ and $2^{nd}$ week), Roxarsone inclusion for one week ($2^{nd}$ week only), restrict feeding, or Roxarsone analogue (3-nitro-4-hydroxyphenyl acid) inclusion. Results showed that feed intake and body weight in the Roxarsone groups and the restrict feeding group decreased significantly during the treatment period. However only the liver and heart weights were significantly decreased (p<0.05) in the restrict feeding group. Fatty acid synthetase (FAS) activity showed a significant decrease (p<0.05) in the Roxarsone groups and the restrict feeding group, two-week-Roxarsone treatment significantly increased NADP-malic dehydrogenase (MDH) activity compared to the restrict (p<0.05). After 2 weeks drug withdrawal, the 1-week-Roxarsone or restrict feeding group showed significantly increased (p<0.05) glucose-6-phosphate dehydrogenase (G-6-PDH) activity (p<0.05). Two-week-Roxarsone treatment significantly decreased (p<0.05) the high density lipoprotein (HDL) and increased (p<0.05) the low density lipoprotein (LDL) and very low density lipoprotein (VLDL) ratio. After drug withdrawal, the 1-week-Roxarsone or restrict feeding group showed significantly increased (p<0.05) creatine kinase (CK) activity. The 2-week-Roxarsone treatment group showed significantly increased (p<0.05) aspartate aminotransferase (AST) activity. The restrict feeding treatment group showed significantly decreased (p<0.05) total protein (TP) concentration. After drug withdrawal, the related enzyme activities in the blood that reflected the liver function were restored to the normal physiological range, except for the total bilirubin concentration and CK activity in the 1-week-Roxarsone group. This group showed a significant increase (p<0.05). Thus, the reasons for liver enlargement in the Roxarsone and restrict feeding groups were different.

Tenofovir Alafenamide for Chronic Hepatitis B Patients with Advanced Fibrosis and Partial Virologic Responses to Oral Nucleos(T)Ide Analogues- Interim Report

( Ming-lung Yu ),( Ming-lun Yeh ),( Chi-yi Chen ),( Pin-nan Cheng ),( Ming-jong Bair ),( Jyh-jou Chen ),( Ching-chu Lo ),( Chi-ming Tai ),( Ching-yang Tsai ),( Kuo-chih Tseng ),( Chien-hung Chen ),( C 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Insufficient data regarding the treatment strategy for partial response to nucleot(s)ide analogue (NUC) raised the aim of investigating tenofovir alafenamide (TAF) switching for chronic hepatitis B (CHB) patients with advanced fibrosis and partial response to other NUCs. Methods: CHB patients with advanced fibrosis (stage 3 or 4) and under NUC (except TAF) therapy with detectable hepatitis B virus (HBV) DNA for >52 weeks are enrolled to TAF 25 mg/day for 96 weeks. The objectives are viral suppression, alanine aminotransferase (ALT) normalization and safety. Results: From Feb. 2019, 34 patients, including 21 (61.8%) with entecavir, 10 (29.4%) TDF and 3 (8.8%) lamivudine or adefovir, were enrolled (15 [44.1%] male, median 53 years). The fibroscan demonstrated a mean of 10.5 kPa (7 [20.6%] cirrhotic). Sixteen (47.1%) patients were HBV e antigen positive, seven (20.6%) had YMDD mutation. The median HBV DNA level declined from 68.5 IU/mL at enrollment to 27.0 IU/mL at 4^th week, and undetectable at 12^th, 24^th, 36^th week, respectively, after TAF switching, with undetectable HBV DNA in 14/34 (41.2%), 17/33 (51.5%), 15/25 (60.0%), and 9/15 (60.0%) patients and rate of ALT normalization (≤40 U/L) of 85.3%, 85.3%, 84.8%, 92.0%, and 80.0%, respectively, after TAF switching. (figure 1) Two patients experienced transient virological breakthrough and another one developed at the final time follow up. Serum creatinine and eGFR levels were stable after TAF switching (figure 1). Two patients early terminated including one at 12^th week due to personal reason, and another one accidently died at 20^th week due to acute heart attack. Others suffered only mild degrees of adverse events which were considered unrelated to treatment. Conclusions: The preliminary results demonstrated the TAF switching is effective and safe in viral suppression for CHB patients with advanced fibrosis and partial virologic responses to other NUCs.