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Munish Kumar Gupta,P. Niesłony,Murat Sarikaya,Mehmet Erdi Korkmaz,Mustafa Kuntoğlu,G. M. Królczyk 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.4
The aerospace and automotive industries make extensive use of aluminium and its alloys. Contrarily, machining of aluminium (Al) alloys presents a number of difficulties, including, but not limited to, poor surface finishing, excessive tool wear, decreased productivity etc. Therefore, it’s very important to measure the machining characteristics during machining of aluminium alloy with sustainable cooling strategies. In this work, a new approach of measurement was adopted to measure the critical geometrical aspects of tool wear, surface roughness, power consumption and microhardness while machining AA2024-T351 alloy under dry, minimum quantity lubrication (MQL), liquid nitrogen (LN2) and carbon dioxide (CO2) cooling conditions. Initially, the various aspects of tool wear were studied with the help of Sensofar Confocal Microscope integrated with Mountains map software and then, the other results such as surface roughness, power consumption and microhardness were measured as per the ISO standards. The outcome of these measurement studies confirms that LN2 and CO2 cooling is helpful in improving the machining characteristics of AA2024-T351 alloy. When compared to dry conditions, the surface roughness values of MQL, LN2, and CO2 all have values that are lowered by 11.90%, 30.95%, and 39.28% respectively, and also power consumption values were lowered by 3.11%, 6.46% and 11.5% for MQL, CO2 and LN2 conditions, respectively.
Serhat Şap,Üsame Ali Usca,Yavuz Selim Tarih,Adem Yar,Mustafa Kuntoğlu,Munish Kumar Gupta 한국정밀공학회 2024 International Journal of Precision Engineering and Vol.11 No.1
Because of their biodegradable and regenerative properties, cellulose nanocrystals derived primarily from naturally occurring cellulose fibers serve as a sustainable and environmentally beneficial material for most applications. Although these nanocrystals are inherently hydrophilic, they can be surface functionalized to suit a wide range of demanding requirements, such as those associated with the creation of high-performance nanocomposites in hydrophobic polymer matrices. Therefore, the present work deals with the application of cellulose-based biodegradable nanocrystals as a lubricant in the machining of PPS composites. In this study, milling process was considered to investigate the influence of the sustainable lubricating conditions on the machinability indexes of PPS composites. As a novel cooling approach, water-based solutions enriched by cellulose nanocrystals with different reinforcements (0.25%, 0.5%, and 1%) were used over known methods such as MQL, conventional flood, and dry. According to the research outcomes, cellulose nanocrystals-based nanofluids provided satisfying contributions on retarding the tool wear and reducing the cutting temperatures considerably. Despite the surface-related results such as roughness, topography and texture are promising for the developed strategy; further investigations will be useful to determine ideal water-particle concentration to improve the quality of the machined surface.
Munish Kumar Gupta,P. Niesłony,Mehmet Erdi Korkmaz,Mustafa Kuntoğlu,G. M. Królczyk,Mustafa Günay,Murat Sarikaya 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.6
Cutting energy must be reduced in order to make machining processes more eco-friendly. More energy was expended for the same amount of material removed, hence a higher specific cutting energy (SCE) implies inefficient material removal. Usually, the type of coolants or lubricants affects the SCE, or the amount of energy needed to cut a given volume of material. Therefore, the present work deals with a study of SCE in the turning of Ti–3Al–2.5V alloy under green cooling strategies. In spite of this, the research effort is also focused on the mechanism of tool wear, surface roughness, and cutting temperature under hybrid cooling, i.e., minimum quantity lubrication (MQL) and cryogenic. The tool wear rate, were explored with tool mapping analysis, and the results were compared with dry, MQL, and liquid nitrogen (LN2) conditions. The tool wear rate analysis claims that the dry condition causes more built up edge (BUE) formation. In addition, the hybrid cooling conditions are helpful in reducing the SCE while machining titanium alloys.
Wahyu Sri Kunto Nugroho,우상욱,김학준 대한화학회 2018 대한화학회지 Vol.62 No.2
Ice-binding proteins have an affinity for ice. They create a gap between the melting and freezing points by inhibiting the growth of ice, known as thermal hysteresis (TH). Interestingly, moderately active LeIBP and hyperactive FfIBP are almost identical in primary and tertiary structures, but differ in TH activity. The TH of FfIBP is tenfold higher than that of LeIBP, due to a subtle difference in their ice-binding motifs. To further evaluate the difference in TH, the interactions were investigated by ice-etching and molecular docking. Ice-etching showed that FfIBP binds to the primary and secondary prism, pyramidal, and basal planes; previously, LeIBP was found to bind to the basal and primary prism planes. Docking analysis using shape complementarity (Sc) showed that the hyperactive FfIBP had higher Sc values for all four ice planes than LeIBP, which is comparable with TH. Docking can be used to describe the hyperactivity of IBPs.
( Wahyu Sri Kunto Nugroho ),( Dong-woo Kim ),( Jong-cheol Han ),( Young Baek Hur ),( Soo-wan Nam ),( Hak Jun Kim ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12
Most cold-adapted enzymes possess higher K<sub>m</sub> and k<sub>cat</sub> values than those of their mesophilic counterparts to maximize the reaction rate. This characteristic is often ascribed to a high structural flexibility and improved dynamics in the active site. However, this may be less convincing to cold-adapted metabolic enzymes, which work at substrate concentrations near K<sub>m</sub>. In this respect, cold adaptation of a shikimate kinase (SK) in the shikimate pathway from psychrophilic Colwellia psychrerythraea (CpSK) was characterized by comparing it with a mesophilic Escherichia coli homolog (EcSK). The optimum temperatures for CpSK and EcSK activity were approximately 30°C and 40°C, respectively. The melting points were 33°C and 45°C for CpSK and EcSK, respectively. The ΔG<sub>H2O</sub> (denaturation in the absence of denaturing agent) values were 3.94 and 5.74 kcal/mol for CpSK and EcSK, respectively. These results indicated that CpSK was a cold-adapted enzyme. However, contrary to typical kinetic data, CpSK had a lower K<sub>m</sub> for its substrate shikimate than most mesophilic SKs, and the k<sub>cat</sub> was not increased. This observation suggested that CpSK may have evolved to exhibit increased substrate affinity at low intracellular concentrations of shikimate in the cold environment. Sequence analysis and homology modeling also showed that some important salt bridges were lost in CpSK, and higher Arg residues around critical Arg 140 seemed to increase flexibility for catalysis. Taken together, these data demonstrate that CpSK exhibits characteristics of cold adaptation with unusual kinetic parameters, which may provide important insights into the cold adaptation of metabolic enzymes.
Nugroho, Wahyu Sri Kunto,Wu, Sangwook,Kim, Hak Jun Korean Chemical Society 2018 대한화학회지 Vol.62 No.2
Ice-binding proteins have an affinity for ice. They create a gap between the melting and freezing points by inhibiting the growth of ice, known as thermal hysteresis (TH). Interestingly, moderately active LeIBP and hyperactive FfIBP are almost identical in primary and tertiary structures, but differ in TH activity. The TH of FfIBP is tenfold higher than that of LeIBP, due to a subtle difference in their ice-binding motifs. To further evaluate the difference in TH, the interactions were investigated by ice-etching and molecular docking. Ice-etching showed that FfIBP binds to the primary and secondary prism, pyramidal, and basal planes; previously, LeIBP was found to bind to the basal and primary prism planes. Docking analysis using shape complementarity (Sc) showed that the hyperactive FfIBP had higher Sc values for all four ice planes than LeIBP, which is comparable with TH. Docking can be used to describe the hyperactivity of IBPs.
Current Advances in Cryopreservation of Microalgae
Nugroho, Wahyu Sri Kunto,Kim, Do-A,Kim, Dong-Woo,Koo, Bon-Won,Hur, Young Baek,Kim, Hak Jun The Korean Society of Marine Life Science 2016 한국해양생명과학회지 Vol.1 No.1
Microalgae are of significant importance for future biotechnological applications. Many microalgae banks or laboratories attempt to maintain various microalgae for further research purposes. Cryopreservation has been preferred to reduce a labor-intensive and costly routine sub-culturing. Cryopreservation can also diminish the genetic drift risk. However, cryopreservation as a long term storage of microalgae method are still in developing progress because it cannot be generalized for all microalgae. Microalgae types, cryoprotectant agents (CPAs) types, freezing and thawing methods are the most important factors that should be considered for cryopreservation. In this short review the basic principles and the current advanced of microalgae cryopreservation methods are discussed with a suggested starting parameters for microalgae cryopreservation.