검은비늘버섯 품종 '진황'의 특성 및 재배법

공원식 외 한국버섯학회 2006 한국버섯학회지 Vol.4 No.3

우수한 약리기능성을 보이는 검은비늘버섯의 병재배에 적합한 새로운 품종 진황을 교잡육종법으로 육성하였다. 진황은 포플라톱밥을 이용한 병재배와 폐면을 이용한 상자재배가 모두 가능하여 재배농가에서 생산이 용이하나, 약용 또는 기능성버섯으로서의 인지도가 낮으므로 소비확대를 위한 약리효과의 홍보가 필요하다. 진황 검은비늘버섯의 주요특성은 다음과 같다. 가. 균사배양 최적온도는 25℃, 자실체발생 및 생육온도는 15~18℃ 이다. 나. 자실체의 색깔은 황갈색이며 갓표면에 흰색~갈색의 인피가 덮혀 있으며 점성이 있다. 다. 병당 유효개체수가 평균 26개로 많고 대도 굵어 품질이 양호하다. A new commercial strain 'Jinhwang' of Pholiota adiposa was developed by hyphal anastomosis. It was developed with crossing between monokaryotic strains derived from ASI 24001 and ASI 24004. The optimum temperature of mycelial growth was 25℃ and that of fruiting body development was 15~18℃. The color of pileus was yellowish brown and many scales and gelatinous sheath were on cap. Average number of individuals a bottle was 26 and its stipe was thick. P. adiposa can be produced by box cultivation using cotton waste.

기능성버섯 장수버섯의 신품종 『장생』

공원식 외 한국버섯학회 2005 한국버섯학회지 Vol.3 No.4

새로운 약용버섯 품목을 개발하여 농가소득증대에 기여하기 위하여 항산화활성을 비롯한 여러 가지 기능성을 가지고 있는 장수버섯의 단핵균주간 교잡으로 육성된 신품종 장생장수버섯의 주요특성은 다음과 같다. 가. 균사배양 최적온도는 30℃, 자실체발생 및 생육온도는 25~30℃이다. 나. 자실체의 색깔은 적갈색이며, 형태는 편평 신장형으로 영지와 유사하게 생겼으나 대가 없는 특징을 가지고 있다. 다. 자실체의 육질이 질기고 단단하며 개체중이 무거워 병재배 병당 수량이 136 g/1000ml로 높은 편이었다. 라. 장생장수버섯은 약용버섯으로 영지와 동일하게 다려서 음용하는데 영지와는 달리 쓴 맛이 없는 특징이 있다. A new hinctional mushroom cultivated variety "Jangsaeng" of Fomitella fraxinea was developed by crossing monokaryons derived from collected strains for contribution to mushroom farmers. The major characteristics of this mushroom were compared to Yeongji 2ho of Ganoderma lucidum because of their morphological similarities. The optimal temperature of mycelial growth and fruitbody sprouting and growth were 30 and 25~30, respectively. The surface color of fruitbody pilus was reddish brown and the fruitbody was flat kidney-shaped Like Ganoderma lucidum except that the stipe was absent Its texture was so tough and rigid that the yield per 1,000 ml bottle might be as high as 136 g. The hot water extract of this medicinal mushroom is fit to drink just like Ganoderma lucidum but its taste is not bitter.

Di-mono 교잡에 의한 잎새버섯 품종 "함박"의 특성 및 재배

공원식 외 한국버섯학회 2007 한국버섯학회지 Vol.5 No.1

우수한 약리기능성을 보이는 잎새버섯의 병재배에 적합한 새로운 품종 "함박"을 이핵단핵 (di-mono) 교잡육종법으로 육성하였다. "함박" 잎새버섯의 재배는 참나무 톱밥 75% + 포플라톱밥 25%에 영양원으로는 미강 15% 또는 옥수수피 10%를 혼합사용할 때 가장 좋았으며, 병재배와 봉지재배가 모두 가능하다. 그러나 재배농가에서 현재 재배되는 버섯을 대체하기에는 배양기간이 길고, 재배시 습도관리가 까다로워 재배법을 더 개선할 필요가 있다. 무엇보다 시장을 형성하기 위해서는 잎새버섯의 약리 기능성에 관한 연구와 이를 바탕으로한 지속적인 홍보가 필요하다. 함박 잎새버섯의 특성은 다음과 같다. 균사배양 최적온도는 25℃, 자실체발생온도는 15∼16℃, 생육온도는 15∼18℃로 기존품종에 비하여 다소 낮은 중온성이다. 함박 자실체의 색깔은 갈색이며 갓 이면에는 장타원형의 관공으로 되어 있다. 병재배시 초발이소요일수가 잎새1호보다 2일 빠르고 수량은 병당 97g으로 높으며, 자실체 송이의 크기가 크며 색택이 진하여 품질이 좋다. A new commercial strain "Hambak" of Grifola frondosa was developed by di-mono crossing between dikaryon of ASI 9031 and monokaryotic strain derived from ASI 9021. It can be cultivated in plastic bottle and bag filled with oak and poplar sawdust substrate which supplemented with 15% rice bran or 10% corn bran. Improvement of cultivation methods was required for shortage of mycelium incubation period and management of humidity in growing room. The optimum temperature of mycelial growth was 25℃ and that of fruiting body development was 15∼18℃. The color of pileus surface was brown and fine pores, whitish to cream-colored, were underside. Primordia formation of Hambak was two days faster than that of Yipsae 1. The yield was 97g/ 850㎖ bottle, a bunch of fruiting body was bigger and the quality was better than Yipsae 1.

근연간 교배방법에 의한 팽이 단핵균주의 교배형분석 및 균주 육성

공원식 외 한국버섯학회 2003 한국버섯학회지 Vol.1 No.1

새로운 백색 팽이 품종을 육성하고자 수집균주 중 백색자실체를 생성하는 5개의 이핵계통을 선발하고 이들로부터 단핵균주를 분리하였다. 분리된 단핵균주 계통의 교배형을 검정한 결과 A1B1A2B2 이었으며, 이핵균주들에 비하여 균사생장 속도가 느리고 변이폭도 좁다는 것을 알 수 있었다. 분리된 단핵계통의 근연간 교배를 수행한 결과 육성된 모든 이핵균주는 백색이었으며 모본보다 우수한 균주들을 선발할 수 있었다. 특히 Fv-4균주는 가장 높은 수량성과 안정성을 나타내었다. 또한 상호교배에 의해 일부 교배조합에서 세포질효과를 확인할 수 있었다. 따라서 근연간 교배법은 기존 재배품종들이 가진 백색과 같은 특성을 유지하면서 새로운 품종을 육성하기 위한 방법으로 사용할 수 있을 것으로 생각된다. To breed new superior strains, collected strains were characterized and then several white strains were selected as parents. Monokaryons from the parents were isolated and studied. All tested white strains showed same mating genotype. Growth rate of monokaryons were lower than collected dikaryons. New dikaryotic strains were derived from inbreeding method, which means mating between monokaryotic isolates from different white strains having same mating genotype. Some of them showed higher yields of fruitbody than their parents. Specially Fv 4-1 strain showed the best productivity. Furthermore some mating combination showed cytoplasmic effect, when they mated reciprocally.

기능성 킬레이트 고분자 Ⅳ -VSA-VP-DVB 킬레이트 수지의 이온교환 특성-

김공수, 심상무, 김경호, 김영식 忠北大學校 1986 論文集 Vol.32 No.-

-본문참고-

A Forwarding Pointer-Based Location Cache Scheme in PCS Networks

Kong, Ki-Sik,Gil, Joon-Min,Han, Youn-Hee,Song, Ui-Sung,Hwang, Chong-Sun 고려대학교 컴퓨터과학기술연구소 2002 JOURNAL OF COMPUTER SCIENCE & ENGINEERING TECHNOLO Vol.4 No.-

This paper proposes a forwarding pointer-based location cache scheme (PB-Cache scheme) that can reduce the signaling cost for location management as compared to the IS-41 standard in Personal Communications Service (PCS) Networks. In the existing cache scheme, the use of cache information can effectively reduce the signaling traffic for locating frequently called mobile users. However, when the cache information is obsolete, it results in much more signaling traffic than that of the existing schemes. In order to solve this problem, the PB-Cache scheme exploits a user's movement locality as well as a user's tall locality at the same time. Even if the cached location information of the called user is not up-to-date, the called user can be found by tracing forwarding pointers in the VLR's without querying the HLR. Thus, the PB-Cache scheme can effectively reduce the signaling traffic for location management, and makes it possible to establish a rapid call setup. Besides, it distributes the signaling and database access load on the HLR to the VLR's. An analytical model is also proposed to compare the performance of the PB-Cache scheme with that of the existing cache scheme and the IS-41 standard. The analytical results indicate that the PB-Cache scheme significantly outperforms the other schemes when the tall-to-mobility ratio is high or the signaling traffic to the HLR is heavy.

Molecular Marker Related to Fruitbody Color of Flammulina velutipes

Kong, Won-Sik,You, Chang-Hyun,Yoo, Young-Bok,Kim, Gyu-Hyun,Kim, Kwang-Ho The Korean Society of Mycology 2004 Mycobiology Vol.32 No.1

White and brown strains of Flammulina velutipes were inter-crossed. All $F_1$ showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.

Event-specific Qualitative and Quantitative Polymerase Chain Reaction Methods for Detection of Insect-resistant Genetically Modified Chinese Cabbage Based on the 3'-junction of the Insertion Site

Kong-Sik Shin,Myung-Ho Lim,Hee-Jong Woo,Sun-Hyung Lim,Hong-Il Ahn,Jin-Hyoung Lee,Hyun-Suk Cho,Soon-Jong Kweon,Seok Cheol Suh 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.3

Transgenic Chinese cabbage 416-3 was developed in Korea by a transformation event involving modified insectresistant gene (cry1Ac1). To monitor unintended release of genetically modified (GM) Chinese cabbage in the future, as well as to meet GM-labeling requirements, the development of a reliable method for detection of GM cabbage is requisite. To develop qualitative and quantitative polymerase chain reaction methods for the insect-resistant GM Chinese cabbage, a cytosolic glutathione reductase (BcgGR1) gene was used as the endogenous reference gene. Primer pairs CGR-1/-2, amplifying the Chinese cabbage endogenous gene, yielded an expected amplicon of 121bp, whereas no amplified product was observed when DNA samples from seven non-cabbage plants were used as templates. The event-specific primer pairs amplifying the junction site between the endogenous genome sequence and the transferred DNA of GM event 416-3, produced amplicons of desired size by qualitative polymerase chain reaction (PCR) assay. An eventspecific quantitative PCR detection method was established using a TaqMan probe and a standard plasmid as a reference molecule,which contained both endogenous and event-specific sequences. For the validation of this method, three different compositions of w/w mixed samples (containing transgenic DNA at 5, 1, and 0.5%of total DNA in the control samples) were quantified. The precision, expressed as standard deviation (SD) and relative standard deviations (RSD), deviated by 0.03–0.26% and 4.75–8.06%, respectively. These results clearly demonstrate that the PCR methods developed herein can be used for event-specific qualitative and quantitative testings of insect-resistant GM Chinese cabbage.

Creep Life Prediction for Udimet 720 Material Using the Initial Strain Method (ISM)

Kong, Yu-Sik,Yoon, Han-Ki,Oh, Sae-Kyoo The Korean Society of Mechanical Engineers 2003 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.17 No.4

Despite of considerable research results or uniaxial tension creep available for superalloys, few studies have been made on high temperature creep using the Initial Stram Method (ISM) In this paper, the real-time prediction of high temperature creep strength and creep lift for the nickel-based superalloy Udimet 720 (high-temperature and high-pressure gas turbine engine materials) was performed on round-bar type specimens under pure static load at the temperatures of 538$^{\circ}C$. 649$^{\circ}C$, and 704$^{\circ}C$. The predictive equation derived from the ISM in creep tests showed better reliability than those from LMP (Larson-Miller Parameter) and LMP-lSM (Larson Miller Parameter-Initial Strain Method) specially for long time creep prediction (10$^3$∼10$\^$5/h).

Terahertz radiation using log-spiral-based low-temperature-grown InGaAs photoconductive antenna pumped by mode-locked Yb-doped fiber laser

Kong, Moon Sik,Kim, Ji Su,Han, Sang Pil,Kim, Namje,Moon, Kiwon,Park, Kyung Hyun,Jeon, Min Yong The Optical Society 2016 Optics express Vol.24 No.7