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Kihyuck Choi,Geun Ju Son,Shabir Ahmad,Seung Yeup Lee,Hyoung Ju Lee,Seon-Woo Lee 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.4
Bacterial traits for virulence of Ralstonia solanacearum causing lethal wilt in plants were extensively studied but are not yet fully understood. Other than the known virulence factors of Ralstonia solanacearum, this study aimed to identify the novel gene(s) contributing to bacterial virulence of R. solanacearum. Among the transposon-inserted mutants that were previously generated, we selected mutant SL341F12 strain produced exopolysaccharide equivalent to wild type strain but showed reduced virulence compared to wild type. In this mutant, a transposon was found to disrupt the murI gene encoding glutamate racemase which converts L-glutamate to D-glutamate. SL341F12 lost its motility, and its virulence in the tomato plant was markedly diminished compared to that of the wild type. The altered phenotypes of SL341F12 were restored by introducing a full-length murI gene. The expression of genes required for flagella assembly was significantly reduced in SL341F12 compared to that of the wild type or complemented strain, indicating that the loss of bacterial motility in the mutant was due to reduced flagella assembly. A dramatic reduction of the mutant population compared to its wild type was apparent in planta (i.e., root) than its wild type but not in soil and rhizosphere. This may contribute to the impaired virulence in the mutant strain. Accordingly, we concluded that murI in R. solanacearum may be involved in controlling flagella assembly and consequently, the mutation affects bacterial motility and virulence.
Culturing Simpler and Bacterial Wilt Suppressive Microbial Communities from Tomato Rhizosphere
Nazish Roy,Kihyuck Choi,Raees Khan,Seon-Woo Lee 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.4
Plant phenotype is affected by a community of associated microorganisms which requires dissection of the functional fraction. In this study, we aimed to culture the functionally active fraction of an upland soil microbiome, which can suppress tomato bacterial wilt. The microbiome fraction (MF) from the rhizosphere of Hawaii 7996 treated with an upland soil or forest soil MF was successively cultured in a designed modified M9 (MM9) medium partially mimicking the nutrient composition of tomato root exudates. Bacterial cells were harvested to amplify V3 and V4 regions of 16S rRNA gene for QIIME based sequence analysis and were also treated to Hawaii 7996 prior to Ralstonia solanacearum inoculation. The disease progress indicated that the upland MM9 1st transfer suppressed the bacterial wilt. Community analysis revealed that species richness was declined by successive cultivation of the MF. The upland MM9 1st transfer harbored population of phylum Proteobacteria (98.12%), Bacteriodetes (0.69%), Firmicutes (0.51%), Actinobacteria (0.08%), unidentified (0.54%), Cyanobacteria (0.01%), FBP (0.001%), OD1 (0.001%), Acidobacteria (0.005%). The family Enterobacteriaceae of Proteobacteria was the dominant member (86.76%) of the total population of which genus Enterobacter composed 86.76% making it a potential candidate to suppress bacterial wilt. The results suggest that this mixed culture approach is feasible to harvest microorganisms which may function as biocontrol agents.
Increased B Cell Understanding Puts Improved Vaccine Platforms Just Over the Horizon
Notario Geneva Rose,Kwak Kihyuck 대한면역학회 2022 Immune Network Vol.22 No.6
In the face of an endlessly expanding repertoire of Ags, vaccines are constantly being tested, each more effective than the last. As viruses and other pathogens evolve to become more infectious, the need for efficient and effective vaccines grows daily, which is especially obvious in an era that is still attempting to remove itself from the clutches of the severe acute respiratory syndrome coronavirus 2, the cause of coronavirus pandemic. To continue evolving alongside these pathogens, it is proving increasingly essential to consider one of the main effector cells of the immune system. As one of the chief orchestrators of the humoral immune response, the B cell and other lymphocytes are essential to not only achieving immunity, but also maintaining it, which is the vital objective of every vaccine.
Culturing Simpler and Bacterial Wilt Suppressive Microbial Communities from Tomato Rhizosphere
Roy, Nazish,Choi, Kihyuck,Khan, Raees,Lee, Seon-Woo The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.4
Plant phenotype is affected by a community of associated microorganisms which requires dissection of the functional fraction. In this study, we aimed to culture the functionally active fraction of an upland soil microbiome, which can suppress tomato bacterial wilt. The microbiome fraction (MF) from the rhizosphere of Hawaii 7996 treated with an upland soil or forest soil MF was successively cultured in a designed modified M9 (MM9) medium partially mimicking the nutrient composition of tomato root exudates. Bacterial cells were harvested to amplify V3 and V4 regions of 16S rRNA gene for QIIME based sequence analysis and were also treated to Hawaii 7996 prior to Ralstonia solanacearum inoculation. The disease progress indicated that the upland MM9 $1^{st}$ transfer suppressed the bacterial wilt. Community analysis revealed that species richness was declined by successive cultivation of the MF. The upland MM9 $1^{st}$ transfer harbored population of phylum Proteobacteria (98.12%), Bacteriodetes (0.69%), Firmicutes (0.51%), Actinobacteria (0.08%), unidentified (0.54%), Cyanobacteria (0.01%), FBP (0.001%), OD1 (0.001%), Acidobacteria (0.005%). The family Enterobacteriaceae of Proteobacteria was the dominant member (86.76%) of the total population of which genus Enterobacter composed 86.76% making it a potential candidate to suppress bacterial wilt. The results suggest that this mixed culture approach is feasible to harvest microorganisms which may function as biocontrol agents.
Periorificial dermatitis in children and adults: retrospective analysis
( Tae-rim Kim ),( Kihyuck Shin ),( Woo-il Kim ),( Min-young Yang ),( Won-ku Lee ),( Hoon-soo Kim ),( Byung-soo Kim ),( Moon-bum Kim ),( Hyun-chang Ko ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2
Background: Periorificial dermatitis (POD) is an acneiform or rosacea-like eruption found in both children and adults. Although POD is not a rare disease, its clinical characteristics and course have not been investigated well, especially in children. Objectives: This study aimed to investigate the clinical difference between childhood and adult PODs. Methods: We retrospectively reviewed medical records and clinical photos of 131 patients diagnosed with POD in the Pusan National University Hospital (Busan and Yangsan) for 17 years (2003-2019). Results: Among 131 patients, 23 (17.6%) were children and 108 (82.4%) were adults. The mean age was 9.0 (2.4-17) years and 43.9 (19-79) years, respectively. The female to male ratio was higher in adult POD (2.6:1) than childhood POD (0.9:1). The involvement of periocular area was more frequent in childhood POD (47.8%) than adult POD (17.6%), however involvement rate of perinasal, perioral and extrafacial area were not different. When we compared perioral dermatitis severity index (PODSI), it is higher in adults (4.3) than in children (3.2). Clinical courses in according to age, sex, treatment, and severity were not different in both groups. Conclusion: Though the sample size of childhood POD is small, this study identified clinical difference such as sex ratio, location and severity between childhood POD and adult POD.
김남성(Nam Seong KIM),김기혁(Kihyuck KIM),정영대(Youngdae CHUNG),최창기(Changgi CHOI),이도경(Dohkyeong LEE),이승재(Seungjae LEE),성기석(Geesuk Sung),전영민(Youngmin JHON),성하민(Hamin SUNG),오경환(Kyunghwan OH),김종기(Jongki KIM),박지영 한국생산제조학회 2010 한국공작기계학회 추계학술대회논문집 Vol.2010 No.-
기존 레이저 미세가공기술 한계를 극복하여 LCD/AMOLED 등의 가공물 내부와 표면에 10㎛ ~ 500㎚ 까지의 초정밀청정가공을 하는 기술개발로서 관련된 고부가가치 생산시스템개발 및 시장요구형 50W/300fs/1035㎚/10㎒ 펨토초 광섬유레이저 기술개발을 소개한다. 기술개발은 2010년6월부터 2015년3월말까지 진행할 예정이며 현재 3개월여의 기술개발을 수행하고 있다. 1차년도 기술개발목표는 레이저광원으로서 20㎽/1035㎚/10㎒/300fs의 모드잠금광섬유레이저를 개발하는 것과 응용프로세스개발은 7㎛급의 내부 3차원 초미세 가공과 표면 초미세가공 등이다. (지식경제부 산업원천기술개발사업 프로젝트번호 10037371).