Ki-67 labeling index as a prognostic marker in advanced stomach cancer

Sang Hyuk Seo,Kwang Hee Kim,Sang Hoon Oh,Yunseon Choi,Ki Jung Ahn,Ji Young Lee,Sang Min Lee,박지선,Woo Gyeong Kim 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.96 No.1

Purpose: Proliferation marker Ki-67 is widely used in cancer prognosis prediction. We tried to investigate the role of Ki-67 as a prognostic factor in stomach cancer after surgery in this study. Methods: We retrospectively evaluated 251 patients who underwent curative resection for gastric cancer from 2010 to 2015. In pathologic examination, Ki-67 labeling index was defined as the percentage of Ki-67 antigen positive cells. Prognostic significance of Ki-67 for gastric cancer was evaluated. Disease-free survival (DFS) was assessed as a primary end-point. Results: The median follow-up period was 28.0 months. Thirty-one patients (12.4%) showed Ki-67 labeling index (LI) lower than 25%. Sixty-eight patients (26.6%) showed recurrence during follow-up period. Recurrence was associated with Ki- 67 LI level (≤25%, P = 0.016), and lymph node metastasis status (P = 0.002). High Ki-67 LI level (>25%) was also related to p53 positivity (P < 0.001) and poorly cohesive type (P = 0.002). The 3-year DFS was 69.4%. Low Ki-67 LI level (≤25%) was related with low DFS (47.6% vs. 72.6%, P = 0.016). T stage (P < 0.001), N stage (P = 0.006), lymphovascular invasion (P = 0.010), and neuronal invasion (P = 0.001) also affected the DFS. In addition, T stage (P = 0.03) and Ki-67 LI (P = 0.035) were independent prognostic factors for DFS. In patients treated with adjuvant chemotherapy (n = 239, 93.4%), low Ki-67 (≤25%) was a poor prognostic factor for DFS (P = 0.013). Conclusion: Low Ki-67 LI predicts high rate of progression and low DFS of stomach cancer. Ki-67 LI can be a predictive marker in resected stomach cancer treated with surgery and adjuvant chemotherapy.

간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현

김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5

Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.

Feedback Influence in the Appraisal Process in Korea

Seo,Dong Ki 국제부동산정책학회 2002 토지와건물 Vol.17 No.-

원발성 피부 CD30 양성/ALK 음성 퇴형성 대세포 림프종 1예

이은주,김협,서영준,서기범,이증훈,박장규,김유찬,김춘옥 대한피부과학회 2003 大韓皮膚科學會誌 Vol.41 No.5

Primary cutaneous CD3O(Ki-1) Positive anaplastic large cell Iymphoma(ALCL) is a rare subset of cutaneous Iymphoma. with a much better prognosis. ALCL is a heterogeneous process that may have a T-cell, B-cell, or indeterminant(null) phenotype and which may or may not express the anaplastic Iymphoma kinase(ALK) oncoprotein. We report a case of ALCL in a 72 year old man. About 4 months ago, multiple erythematous firm ulcerative mass and satellite nodules developed on the right lower leg. The skin lesions rapidly increased in number and size. Some lesions became painful and centrally ulcered. The histologic findings showed a diffuse infiltrate of large Iymphocytes with large nuclei, prominent and multiple nucleoli, and ample cytoplasm. Immunohistochemical stainings for CD3O, CD5 were positive but stainings for LCA, CD3, CD45RO, CD2O, cytokeratin, EMA, and ALK were negative. Therefore, we diagnosed our case as CD+/30ALK- ALCL (Korean J Dermatol 2003;41(5) : 666~669)

The Conjugated Double Bond of Coniferyl Aldehyde Is Essential for Heat Shock Factor 1 Mediated Cytotoprotection

Choi, Seul-Ki,Mun, Gil-Im,Choi, Eun,Kim, Seo-Young,Kwon, Youngjoo,Na, Younghwa,Lee, Yun-Sil American Society of Pharmacognosy 2017 Journal of natural products Vol.80 No.8

<P>Coniferyl aldehyde (1) is previously reported as a potent inducer of heat shock factor 1 (HSF1). Here, we further examined the active pharmacophore of 1 for activation of HSF1 using the derivatives coniferyl alcohol (2), 4-hydroxy-3-methoxyphenylpropanal (3), and 4-hydroxy-3-methoxyphenylpropanol (4). Both 1 and 2 resulted in increased survival days after a lethal radiation (IR) dose. The decrease in bone marrow (BM) cellularity and Ki67-positive BM cells by IR was also significantly restored by 1 or 2 in mice. These results suggested that the vinyl moiety of 1 and 2 is necessary for inducing HSF1, which may be useful for developing small molecules for cytoprotection of normal cells against damage by cytotoxic drugs and radiation.</P>

불면을 호소하는 뇌실질내출혈 환자 치험 1례

김경묵,김세원,서유나,정우상,문상관,진철,조기호,권승원,Kim, Gyeong-muk,Kim, Se-won,Seo, Yu-na,Jung, Woo-sang,Moon, Sang-Kwan,Jin, Chul,Cho, Ki-ho,Kwon, Seungwon 대한중풍순환신경학회 2019 대한중풍.순환신경학회지 Vol.20 No.1

A case of a 46-year-old Korean male with post-stroke insomnia that induced by intracranial hemorrhage was presented. He was treated with Transcutaneous electric acupoint stimulation (TEAS) at BL62 and KI6 once per a day for 17 days. We recorded the patient's sleep time and time of asleep, and used PSQI-K to assess severity of patient's insomnia symptom. After using TEAS, there was improvement in patient's insomnia severity and the time of sleep. From the result of this case, electrical stimulation at acupoint can may be effective in treating post-stroke insomnia.

Focal adhesion kinase and src expression in premalignant and malignant skin lesions

( Ju Yeon Choi ),( Jae Yun Lim ),( Han Saem Kim ),( Jung Min ),( Jung In Kim ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Chong Won Choi ),( Yoon Hwan Kim ),( Jin Hee Sohn ),( Hyunjoo Lee ),( Won Serk Ki 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Focal adhesion kinase (FAK) and Src are non-receptor tyrosine kinases. FAK and Src play a critical role in inducing malignant transformation in tumor cells. Objectives: We performed immunohistochemical staining for total and phosphorylated forms of FAK and Src, to evaluate the role of FAK and Src in the development of premalignant and malignant skin lesions. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Results: Cells positive for t-Src, p-Src-y530, t-FAK and pFAK-s722 were detected in premalignant intra-epithelial lesions (PELs) and squamous cell carcinomas (SCCs), but not in the perilesional skin. There was a tendency towards high correlation between Ki-67 and t-FAK or pFAK-s722, suggestive of the active role of FAK in cell proliferation. Conclusion: Our findings of higher t-Src and p-Src-y530 positive cells in PELs, as compared to SCCs (with higher Ki-67 level), are suggestive of the other role of Src in tumor formation and progression, which requires further investigation.

Allyl Isothiocyanate가 미생물의 생육에 미치는 영향

서권일,강갑석,심기환 東國專門大學 1996 金龜論叢 Vol.3 No.1

십자화과 채소에 존재하는 AIT의 항균성 및 식품보존제로의 이용가능성을 조사하기 위하여 AIT가 미생물의 증식에 미치는 영향 및 AIT처리 미생물의 형태변화를 현미경으로 관찰한 결과는 다음과 갈다. 미생물이 첨가된 배지에 AIT를 200ppm의 농도로 첨가한 후 24시간 경과시는 시험균주 모두에서 미생물의 성장저해를 50%이상 나타내었으며, 800ppm의 농도에서 Lactobacillus brevis는 36시간, 나머지 시험균주들은 48시간까지 균의 성장이 완전 억제되었다. 각종 시험균주에 대한 MIC는 E. coli와 Vibrio parahaemolyticus가 220ppm, Lactobacillus brevis와 Saccharomyces cerevisiae가 400ppm이었으며, Aspergillus oryzae는 800ppm이었다. AIT로 처리한 E. coli균주를 전자현미경으로 관찰한 결과 균체표면이 수축되고 표층구조가 허물어져 심한 형태학적 변화를 나타내었다.

직업교육 향상을 위한 애완동물 산업의 실태분석에 관한 연구

서국성,고기환,신정남 啓明專門大學 産業開發硏究所 2002 啓明硏究論叢 Vol.20 No.1

본 연구는 매년 크게 성장하고 있는 애견 산업의 분야별 특성과 자격증 제도에 대한 현황을 조사한 후 졸업생들의 취업지도를 활성화시키기 위하여 실시하였다. 취업에 필요한 기초자격증은 공인 트리머 자격증(1, 2, 3급. 교사, 사범)과 애견 구루머 자격증(1, 2, 3급, 교사, 사범)이 있으며. 그 밖에도 경비견 훈련사 자격증도 있다 보다 전문적인 자격증으로서는 Breeder자격증, Handler자격증 등이 있는데 학교에서 이론강의와 기초실습을 충실히 익힌 후 관련산업체에서 철저한 현장실습 수행과정을 이수하는 것이 필요한 것으로 조사되었다. This study was investigated the present condition on a certificates of qualification and characteristic of pet dog industry that it is greatly growing each year. in order to improve the employment of college graduates. The basic certificate of qualification for the employment are the official trimmer licensed(1, 2, 3 class, teacher and master) and pet groomer license (1, 2, 3 class teacher and master). There is also a certificate of the trainer of the guard dog. To obtain the these certificates of qualification, the study on the pet dog, that is lectures and basic practices at school and good teaching practice in grooming and trimming is necessary.

실시간 엘리베이터 감시 및 제어 시스템의 설계 방법론

서현곤,김기형 嶺南大學校 工業技術硏究所 1998 工業技術硏究所論文集 Vol.26 No.2

As modern buildings become larger and higher, the number of elevators to be controlled in buildings also increases. For efficient management of the elevators in a building, a real-time elevator status monitor and control system is essential. In this paper, we present a design methodology of the real time elevator monitor and control(EMC) system. The EMC system is designed to be connected directly to the internal network of elevator systems, thereby efficient monitoring and control can be possible.