전립선암에서 TGFβl, TGFβ2 발현과 Ki-67 표지지수의 예후적 가치

장혁준,김기권,이경섭 大韓泌尿器科學會 1999 Korean Journal of Urology Vol.40 No.5

The oncoprotein, gankyrin, is up-regulated in middle ear cholesteatoma

Kim, Ki Hyun,Lim, Hye Jin,Kim, Yeon Ju,Kim, Seung Won,Kim, Young Sun,Tian, Chunjie,Park, Keehyun,Park, Tae Jun,Choung, Yun-Hoon Scandinavian University Press 2014 Acta oto-laryngologica Vol.134 No.3

<P><I>Conclusion:</I> Gankyrin seems to be a better biomarker for cholesteatoma compared with Ki-67. <I>Objective:</I> Gankyrin is an oncoprotein, and occurs in cancers but not in benign diseases. The goal of this study was to compare expression of gankyrin, p53, and a proliferation marker (Ki-67) in cholesteatoma and retroauricular skin (RAS), and to evaluate their significance as clinical parameters. <I>Methods:</I> The levels of expression of gankyrin, Ki-67, and p53 in 10 cholesteatoma and 10 paired samples of normal RAS were evaluated by immunohistochemical staining and Western blot. The results were compared with clinical profiles to investigate a correlation. <I>Results:</I> The expression of gankyrin, Ki-67, and p53 proteins was observed in both basal and suprabasal layers of cholesteatoma. The intensity of gankyrin expression was ‘positive’ in two cases (20%) and ‘strongly positive’ in eight cases (80%); p53 expression in the suprabasal layer was ‘positive’ in 70% of cases; and the Ki-67 staining was ‘focal’ in 80% of cases. In RAS, these proteins were expressed dominantly in the basal layer. Western blot analysis showed that the gankyrin band was more intense in cholesteatoma than in RAS for three of four cases (<I>p</I> < 0.05). However, there was no significant difference in the expression of gankyrin, Ki-67, and p53 according to clinical variables.</P>

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

Seasonal parasitism of Riptortus clavatus Thunberg (Heteroptera: Alydidae) by Dionaea magnifrons (Herting) (Diptera: Tachinidae)

Jun Ki Son,Nguyen Xu Do,박정규 한국응용곤충학회 2008 Journal of Asia-Pacific Entomology Vol.11 No.4

Seasonal parasitism of adult Riptortus clavatus (Thunberg) (Heteroptera: Alydidae) by the tachinid fly parasitoid, Dionaea magnifrons (Herting), was monitored at three sites in Korea, 2006 by using the aggregation pheromone traps of R. clavatus. Basic developmental characteristics of the parasitoid were also examined. Annual parasitism of R. clavatus female and male adults was 6.8 and 11.6%, respectively. Parasitism of females and males of the over-wintering (Oct. to Jun.) generation (10.6 and 16.5%, respectively) was higher than the summer (Jul. to Sep.) generation (2.1 and 2.5%). Maximum parasitism for males was recorded during spring or autumn, reaching 35.6, 25.0, and 26.9% at the university campus, and pear and sweet persimmon orchards, respectively. Sex ratio of all the D. magnifrons was slightly male-dominated (62.2%). The pupal period of D. magnifrons was 9.2 days for both sexes in room temperature conditions. D. magnifrons larvae escaped from R. clavatus in 8.0±3.4 days after host bug collection fromthe field during April to September, whereas in October and November they emerged in 15.1±3.8 days. This parasitoid fly should be studied further to determine its effectiveness as a biological control agent complementary to existing strategies in the management of bean bug. Seasonal parasitism of adult Riptortus clavatus (Thunberg) (Heteroptera: Alydidae) by the tachinid fly parasitoid, Dionaea magnifrons (Herting), was monitored at three sites in Korea, 2006 by using the aggregation pheromone traps of R. clavatus. Basic developmental characteristics of the parasitoid were also examined. Annual parasitism of R. clavatus female and male adults was 6.8 and 11.6%, respectively. Parasitism of females and males of the over-wintering (Oct. to Jun.) generation (10.6 and 16.5%, respectively) was higher than the summer (Jul. to Sep.) generation (2.1 and 2.5%). Maximum parasitism for males was recorded during spring or autumn, reaching 35.6, 25.0, and 26.9% at the university campus, and pear and sweet persimmon orchards, respectively. Sex ratio of all the D. magnifrons was slightly male-dominated (62.2%). The pupal period of D. magnifrons was 9.2 days for both sexes in room temperature conditions. D. magnifrons larvae escaped from R. clavatus in 8.0±3.4 days after host bug collection fromthe field during April to September, whereas in October and November they emerged in 15.1±3.8 days. This parasitoid fly should be studied further to determine its effectiveness as a biological control agent complementary to existing strategies in the management of bean bug.

개에서 발생한 수컷 가성 반음양 1례

이기찬,손정민,윤기영,최호정,박성준,이영원,정성목,신상태,조종기 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2006 動物醫科學硏究誌 Vol.14 No.-

In this report, we present a case of the male pseudohermaphrodite in a beagle dog. The animal was subjected for examination due to enlargement of clitoris that was shown as reddish finger-like structure protruding from the vulva. Visual examination of the internal genitalia revealed that the animal possessed both testes and uterus. Based on the findings of physical examination and exploratory laparotomy, it was tentatively diagnosed as male pseudohermaphrodite. Internal genitalia and enlarged clitoris were removed for the prevention of pyometra and Sertoli cell tumor.

Purification and Characterization of Thermostable β-Glucosidase from the Brown-Rot Basidiomycete Fomitopsis palustris Grown on Microcrystalline Cellulose

Jeong-Jun Yoon,Ki-Yeon Kim,Chang-Jun Cha 한국미생물학회 2008 The journal of microbiology Vol.46 No.1

An extracellular β-glucosidase was purified 154-fold to electrophoretic homogeneity from the brown-rot basidiomycete Fomitopsis palustris grown on 2.0% microcrystalline cellulose. SDS-polyacrylamide gel electrophoresis gel gave a single protein band and the molecular mass of purified enzyme was estimated to be approximately 138 kDa. The amino acid sequences of the proteolytic fragments determined by nano-LC- MS/MS suggested that the protein has high homology with fungal β-glucosidases that belong to glycosyl hydrolase family 3. The Kms for p-nitorophenyl-β-D-glucoside (p-NPG) and cellobiose hydrolyses were 0.117 and 4.81 mM, and the Kcat values were 721 and 101.8 per sec, respectively. The enzyme was competitively inhibited by both glucose (Ki= 0.35 mM) and gluconolactone (Ki= 0.008 mM), when p-NPG was used as substrate. The optimal activity of the purified β-glucosidase was observed at pH 4.5 and 70°C. The F. palustris protein exhibited half-lives of 97 h at 55°C and 15 h at 65°C, indicating some degree of thermostability. The enzyme has high activity against p-NPG and cellobiose but has very little or no activity against p-nitrophenyl-β-lactoside, p-nitrophenyl-β-xyloside, p-nitrophenyl-α-arabinofuranoside, xylan, and carboxymethyl cellulose. Thus, our results revealed that the β-glucosidase from F. palustris can be classified as an aryl- β-glucosidase with cellobiase activity.

특성화고등학교 학생들의 과학 수업에서의 흥미에 관한 내러티브 연구: 전공 교과목과의 비교를 중심으로

장준규 ( Jun-kyu Chang ),이준기 ( Jun-ki Lee ) 전북대학교 과학교육연구소 2023 과학과 과학교육 논문지 Vol.48 No.1

이 연구는 특성화 고등학교에 입학한 학생 4명의 자발적 참여를 통해 이루어 졌다. 이 연구 대상자들이 어떠한 부분에서 과학에 대한 흥미를 경험했고, 무엇이 과학에 대한 흥미를 잃게 되었는지에 대해 연구 참여자들의 관점에서 확인하고 분석하고자 내러티브 탐구 방법을 통해 깊이 있는 면담을 진행했다. 이 연구의 목적은 특성화 고등학교 학생들이 과학에 흥미를 잃게 하는 다양한 요인들을 파악하는데 있다. 본 연구의 주요 결론을 제시하면 다음과 같다. 첫째, 연구 참여 학생들은 초 · 중학교시기에 연구 참여 학생들은 과학에 대한 흥미를 어느 정도 가지고 있었지만 특성화 고등학교에 입학 후 잃게 되었다. 둘째, 학생들이 과학교과에 흥미를 느낄 수 있게 해주는 환경이 조성되지 못했다. 결론을 종합하면 연구 참여 학생들은 과학교과에 대한 필요성을 인지하고 있음에도 불구하고, 과학교과에 대한 흥미를 느끼지 못해 수업에 집중할 수 없었다. 또한 연구 참여학생들의 과학교과는 일관적인 이론수업으로 진행되었고, 그 과정에서 학생들의 이해도는 고려되지 않았다. 결국 학생들은 과학교과에 대해 어려움을 느꼈고 흥미를 잃게 되었다. This study was conducted through the voluntary participation of four students who entered a vocational high school. The purpose of this research was to identify various factors that cause students at vocational high schools to lose interest in science, by examining and analyzing from the perspectives of the research participants which parts of science they experienced an interest in and what caused them to lose interest. In-depth interviews were conducted using narrative inquiry method. The main findings of this study are as follows. First, the participating students had some interest in science during their elementary and middle school years, but lost it after entering the vocational high school. Second, the environment that allows students to develop an interest in science was not provided. In conclusion, although the participating students were aware of the importance of science, they could not focus on class because they did not have an interest in science. In addition, their science classes consisted of consistent theoretical lessons, without considering the students' level of understanding. As a result, the students found science classes difficult and lost interest.

Reversal cell proliferation by exercise in the hippocampal dentate gyrus of offspring born from the stress-exposed maternal rats during pregnancy

Joon-Ki Park,Sam-jun Lee 한국스포츠학회 2015 한국스포츠학회지 Vol.13 No.4

임신중 소음스트레스를 받은 어미 쥐로부터 태어난 새끼 쥐의 뇌의 해마 치상회에서 세포분화의 표식인자인 Ki-67 과 세포분화 촉진요소인 NPY의 발현에 운동이 미치는 영향을 연구하기 위한 목적으로 교미중의 어미 쥐에서 스트레스에 노출시켜 태어난 새끼 쥐를 4집단으로 (통제군, 운동군, 스트레스군, 스트레스 및 운동군)으로 분류하여 각각 집단별 10마리로 분류를 하여 트레드밀 운동을 1주간 실시를 하였다. 운동은 태어난지 3주에서 일주일동안 시키고 희생을 시켰다. 분석은 면역조직화학법을 이용하여 분석을 하였으며, 운동은 1회 30분, 일주일로써 최초 5m/min으로 5분, 다음 8m/min으로 5분 이후 마지막으로 10m/mim으로 20분 동안 경사 없이 중정도의 강도로 실시를 하였다. 통계적인 분석은 일원변량분석을 실시하였으며, 유의수준은 .05로 설정하였다. 결과로는 통제군과 스트레스군보다 운동군과 스트레스 및 운동을 복합한 집단에서 Ki-67 및 NPY의 발현에서 유의한 차이를 보였다. 이러한 유의한 차이가 의미하는 것은 운동이 스트레스를 받고 태어난 새끼 쥐의 뇌의 세포분화의 감소를 운동을 통하여 억제시킴으로써 운동의 긍정적인 효과를 주는 것으로 볼 수가 있다.

흰쥐에서 음경발기 평가의 척도로서 음경해면체내압측정술 확립

송윤섭,김용준,이광우,김준모,문기혁,박영호,민영기,유형균,김형건 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Because studies about the control of penile erection at the entral nervous system was dependent on the animal experiments, establishment of measuring penile erection is important to evaluate the effects changes at the central nervous system on the penile erection. Intracavernous pressure measurement in rats has been introduced as a suitable index for the evaluation of penile erection but this method is invasive and technically difficult. So, we established the intracavernous pressure measurement in rats as the experimental index for penile erection. Materials and Methods: 42 male adult Sprague-Dawley rats (250-300gm) were divided to saline or papaverine treated group. Rats were placed on a heating table to maintain their body temparature and anesthetized with 50mg/kg of pentobarbital sodium by intraperitoneal injection. A cannula was inserted to the left carotid artery to measure systemic blood pressure. Saline (0.05, 0.1ml), papaverine(0.05, 0.1, 0.25, 0.5, 1.0, 2.0mg) were injected intracavernously via 26 guage needle filled with saline or papaverine which connected to pressure transducer and polygraph was inserted into the corpus cavernosum on one side to inject the drug and to measure intracavernous presure. Results: Properly executed insertion and intracavernous administration produced a instantaneous but transient rise in intracavernous pressure that substantially stabilized and maintained at 5.4±0.4mmHg. Intracavernous pressure and duration of penile blood flow following intracavernous injection of papaverine are increased comared to those of saline. Conclusions: Monitoring intracavernous pressure in rats represents a suitable index for the evaluation of penile erection in small laboratory animals.

BMB Reports : Connexin32 inhibits gastric carcinogenesis through cell cycle arrest and altered expression of p21^Cip1 and p27^Kip1

( Hyang Jee ),( Su Hyung Lee ),( Jun Won Park ),( Bo Ram Lee ),( Ki Taek Nam ),( Dae Yong Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.1

Gap junctions and their structural proteins, connexins (Cxs), have been implicated in carcinogenesis. To explore the involvement of Cx32 in gastric carcinogenesis, immunochemical analysis of Cx32 and proliferation marker Ki67 using tissue-microarrayed human gastric cancer and normal tissues was performed. In addition, after Cx32 overexpression in the human gastric cancer cell line AGS, cell proliferation, cell cycle analyses, and p21^Cip1 and p27^Kip1 expression levels were examined by bromodeoxyuridine assay, flow cytometry, real-time RT-PCR, and western blotting. Immunohistochemical study noted a strong inverse correlation between Cx32 and Ki67 expression pattern as well as their location. In vitro, overexpression of Cx32 in AGS cells inhibited cell proliferation significantly. G1 arrest, up-regulation of cell cycle-regulatory proteins p21^Cip1 and p27^Kip1 was also found at both mRNA and protein levels. Taken together, Cx32 plays some roles in gastric cancer development by inhibiting gastric cancer cell proliferation through cell cycle arrest and cell cycle regulatory proteins. [BMB Reports 2013; 46(1): 25-30]