Systemic administration of low dosage of tetanus toxin decreases cell proliferation and neuroblast differentiation in the mouse hippocampal dentate gyrus

Bing Chun Yan,In Hye Kim,Joon Ha Park,Ji Hyeon Ahn,Jeong-Hwi Cho,Bai Hui Chen,Jae-Chul Lee,Jung Hoon Choi,Ki-Yeon Yoo,Choong Hyun Lee,Jun Hwi Cho,Jong-Dai Kim,Moo-Ho Won 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.3

In the present study, we investigated the effect of Tetaus toxin (TeT) on cell proliferation and neuroblast differentiation using specific markers: 5-bromo-2-deoxyuridine (BrdU) as an exogenous marker for cell proliferation, Ki-67 as an endogenous marker for cell proliferation and doublecortin (DCX) as a marker for neuroblasts in the mouse hippocampal dentate gyrus (DG) after TeT treatment. Mice were intraperitoneally administered 2.5 and 10 ng/kg TeT and sacrificed 15 days after the treatment. In both the TeT-treated groups, no neuronal death occurred in any layers of the DG using neuronal nuclei (NeuN, a neuron nuclei maker) and Fluoro-Jade B (F-J B, a high-affinity fluorescent marker for the localization of neuronal degeneration). In addition, no significant change in glial activation in both the 2.5 and 10 ng/kg TeT-treated-groups was found by GFAP (a marker for astrocytes) and Iba-1 (a marker for microglia) immunohistochemistry. However, in the 2.5 ng/kg TeT-treated-group, the mean number of BrdU, Ki-67 and DCX immunoreactive cells, respectively, were apparently decreased compared to the control group, and the mean number of each in the 10 ng/kg TeT-treated-group was much more decreased. In addition, processes of DCX-immunoreactive cells, which projected into the molecular layer, were short compared to those in the control group. In brief, our present results show that low dosage (10 ng/kg) TeT treatment apparently decreased cell proliferation and neuroblast differentiation in the mouse hippocampal DG without distinct gliosis as well as any loss of adult neurons.

퇴원한 정신분열병 환자의 외래 통원 치료에 미치는 요인

박기국,원승희,김상헌,김휘동,강병조 대한생물치료정신의학회 1998 생물치료정신의학 Vol.4 No.2

목적 : 저자는 외래 통원 치료를 지속적으로 받는 환자들과 중단한 환자들의 현황을 조사 비교했다. 그리고 외래 통원 치료를 지속적으로 받는 환자들 중 투약 약물 종류에 따른 치료 순응도에 영향을 미칠 수 있는 변인들을 비교했다. 연구 대상과 방법 : 첫 번째 1997년 4월 1일부터 1998년 4월 30일 동안 퇴원한 정신분열병 환자 140명을 대상으로 자료를 조사한 후 분석했다. 두 번째 1998년 5월 18일부터 1998년 6월 17일까지 한달 동안 외래를 방문한 후 순응도 요인에 관한 설문지의 항목을 작성한 46명 환자의 자료를 분석했다. 결과 : 외래 통원 치료를 지속적으로 받는 환자군과 통원 치료를 중단한 환자군을 비교했을 때, 성별, 연령, 교육 수준, 결혼 여부, 직업, 종교, 정신분열병 아형, 발병 연령, 유병 기간, 입원 횟수, 복용 약물들의 하루 총복용 횟수에서 통계학적으로 의미 있는 차이는 없었다. 퇴원 당시 clozapine을 투약했던 환자들에서 외래 치료지속율이 가장 높게 나타났다. 외래 통원 치료를 받는 환자들의 투약 약물 종류군에 따라 경제적 곤란도, 교육 수준, 유병 기간, 입원 횟수에서 유의한 차이가 있었다. 약물 종류군(clozapine, risperidone, typical anti-psychotics)에 따라 경제 상태, 향후 기대 치료 기간, 약물에 대한 인식도, 약물 효과에 대한 기대, 현재 약의 부작용, 약물의 부작용 경험도, 성별, 연령, 결혼 여부, 직업, 종교, 정신분열병 아형, 발병 연령, 1일 약물 복용 총 횟수의 차이가 없었다. 결론 : 클로자핀 약물을 투약한 환자들이 다른 종류의 항정신병 약물 투약 환자들에 비해 외래 통원 치료를 지속적으로 받는 비율이 높았다. Objectives : The authors compared the status of discharged schizophrenics between who are continuously followed and who are not in the treatment. Also we compared compliance factors among administered drug groups of the follow-up schizophrenics. Methods : First, we surveyed and analysed one hundred-four schizophrenics after discharge. Second, we analysed the questionnaires which were written by the forty-six follow-up patients who were asked about compliance factors. Results : There were no significant differences between patients who are continuously follow-up and who are not in the treatment on sex, age, educational level, marriage, occupation, religion, subtypes of schizophrenia, age of onset, duration of illness, admission rate, and complexity of drug administration methods Clozapine administered group had higher follow-up rate than that of other antipsychotics(rispendone and typical antipsychotics) administered groups among the discharged schizophrenics. Among the follow-up patients, there were significant differences between atypical antipsychotics patients and typical antipsychotics patients on economic difficulty, educational level, duration of illness, admission rate. There were no significant differences on the other of demographic and psychiatric characteristics. Conclusion : Clozapine administered group had higher follow-up rate than that of ther antipsychotics administered groups among the discharged schizophrenics.

도장작업시 유해인자들의 노출수준과 작업환경관리실태

이세기,문덕환,이채관,황용식,선병관,손병철,김대환,이창희,김휘동,이채언 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Objectives: For the purpose of preparing the fundamental data and assessing the status of exposure level of chemical substances in painting process. Methods and Materials: The author determined the concentration of organic solvents, lead(Pb), Chromium(Cr), and Toluene-2, 4-diisocyanate(TDI) in air of painting process to 90 industries classified by 15 types of manufacturing industry during a period from Jan. 1st 2001 to Dec. 31st 2001. Results: The results were as follows: 1. The geometric mean level of each chemical substance was 0.1706 ppm for exposure index in organic solvents, 0.0019㎎/㎥ for Pb, 0.0024 ㎎/㎥ for Cr, and 0.0004ppm for TDI, respectively. 2. The exposure level of each chemical substances by type of painting was the highest in dipping and brush painting for organic solvent, in spray-painting for TDI, and in spray gun-painting for Pb and Cr, respectively. 3. The exposure level of each chemical substances by type of local ventilation equipment was higher in enclosed and booth type for organic solvents. Cr, and TDI, and in exterior type for Pb, respectively than those of other types. 4. The exposure level of each chemical substances by type of hazardous material arresting media was higher in using water for organic substances, in using bag filter for Pb, TDI, respectively than those of other types. Conclusion: According to above results, this study suggests that the exposure level by type of industry exceeded the exposure level in wooden furniture and metal furniture manufacturing industry for organic solvents and Pb, respectively.

남녀 대학생의 척추만곡에 관한 연구

남기석,이충휘,이병규 한국전문물리치료학회 1998 한국전문물리치료학회지 Vol.5 No.3

인천 인현동 호프집 화재 피해자 분석

최정태,안무업,안희철,최영미,정재봉,서정열,유기철,이삼우,박석현,조준휘,김성환,김아진,송근정 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Purpose : This study was conducted to develop field triage, transportation, distribution, and prehospital care at a fire disaster by analyzing the victims of the fore that broke out at a bar in Incheon. Method : We analyzed the cases of the victims of a fire in Incheon in Oct. 1999. We determined the primary care hospital, the arrival time, the burn size, the outcome, and the injury type from the medical records, the concerned organ records, and interviews with concerned persons. Result : The total number of victims was 137: 56 prehospital deaths, 1 hospital death, and 80 survivals. The Pearson correlation coefficient between the burn size and the severity was -0.175. There were 121(89.6%) cases of inhalation injury, 59 (43.7%) cases of flame burns, 66 (48.9%) cases of hypoxic brain damage, and 16 (11.9%) cases involving other types of injury. Conclusion : The causes of death of the fire victims were inhalation injury and hypoxic brain damage due to CO poisoning and other toxic inhalants. We propose the use of a simple triage and rapid treatment(START) system and a reassessment the delayed category in fire disasters.

Normally Off Single-Nanoribbon <tex> $\hbox{Al}_{2} \hbox{O}_{3}\hbox{/GaN}$</tex> MISFET

Ki-Sik Im,Ryun-Hwi Kim,Ki-Won Kim,Dong-Seok Kim,Chun Sung Lee,Cristoloveanu, S.,Jung-Hee Lee IEEE 2013 IEEE electron device letters Vol.34 No.1

<P>A single-nanoribbon Al<SUB>2</SUB>O<SUB>3</SUB>/GaN metal-insulator-semiconductor field-effect transistor (MISFET) has been fabricated. The fabricated device exhibits normally off operation with a threshold voltage of 2.1 V. The device also exhibits superior performances such as a maximum drain current density of 1.51 A/mm, a maximum transconductance of 580 mS/mm, and a field-effect mobility of 293 cm<SUP>2</SUP>·V<SUP>-1</SUP>·s<SUP>-1</SUP>. This is because the electron concentration in the GaN channels can be increased due to the enhanced gate controllability, which, thus, effectively screens the field lines from the interface traps or the defects near the channels to improve the electron mobility in the channel. The nanoribbon Al<SUB>2</SUB>O<SUB>3</SUB>/GaN MISFET is a very promising candidate for high-performance normally off GaN FETs.</P>

Reconstruction of microstomia considering their functional status

Ki, Sae Hwi,Jo, Gang Yeon,Yoon, Jinmyung,Choi, Matthew Seung Suk Korean Cleft Palate-Craniofacial Association 2020 Archives of Craniofacial Surgery Vol.21 No.3

Background: Microstomia is defined as a condition with a small sized-mouth that results in functional impairment such as difficulty with food intake, pronunciation, and poor oral hygiene and cosmetic problems. Several treatment methods for microstomia have been proposed. None of them are universally applicable. This study aims at analyzing the cases treated at our institution critically reviewing the pertinent literature. Methods: The medical records of all microstomia patients treated in our hospital from November 2015 to April 2018 were reviewed retrospectively. Of these, all patients who received surgical treatment for microstomia were included in the study and analyzed for etiology, chief complaint, surgical method, and outcomes. The functional outcomes of mouth opening and intercommissure distance before and after the surgery were evaluated. The cosmetic results were assessed according to the patients' satisfaction. Results: Five patients with microstomia were corrected. Two cases were due to scar contracture after chemical burn, two cases derived from repeated excision of skin cancer, and one patient suffered sequela of Stevens-Johnson syndrome. The following surgical methods were applied: one full-thickness skin graft on the buccal mucosa, three buccal mucosal advancement flaps after triangular excision of the mouth corner, and one local buccal mucosal flap. Mouth opening was increased by 6.0 mm, and the intercommissure distance improved by 7.2 mm on average. Follow-up was 9.6 months (range, 5-14 months). Cosmetic assessment was as follows: two patients found the results excellent, three judged it as good. Conclusion: Microstomia has several causes. In order to achieve optimal functional recovery and aesthetic improvement it is important to precisely evaluate the etiologic factors and the severity of the impairment and to carefully choose the appropriate surgical method.

Risk and Effectiveness of Using Thrombin in Microvascular Free Tissue Transfer

Ki, Sae Hwi,Kim, Han Joon 대한미세수술학회 2014 Archives of reconstructive microsurgery Vol.23 No.1

Purpose: Recent studies have reported on application of fibrin glue composed of fibrinogen and thrombin to nerve anastomosis, which can be another candidate for vessel anastomosis. However, no research regarding the risk and effectiveness of thrombin in microvascular free tissue transfer has been reported. Therefore, the aim of study is to determine the risk and effectiveness of thrombin on microvascular free tissue transfer through clinical cases. Materials and Methods: Twenty-five patients underwent free flap reconstruction for soft tissue defect or bone exposure in our institute from March 2011 to February 2014. In the group using thrombin, dissolved powder thrombin (5,000 IU/amp) was mixed with 10 mL normal saline. Saline mixed with thrombin was applied on the flap, recipient, and around vessel anastomosis. In the control group, free flap was performed using the same method, except using thrombin. We analyzed the results between the two groups. Results: All flaps survived. The group using thrombin included 14 patients and the control group included 11 patients. Hematoma was found in two cases, respectively, in each group. The group using thrombin showed lower incidence of hematoma than the control group. No difference in survival rate of the flap was observed between the thrombin group and the control group. Conclusion: Results of this study showed that use of saline mixed with thrombin in free tissue transfer may be safe and effective for prevention of hematoma formation in the recipient site.

Reconstruction of Fingertip Amputation: Necrosis Is Expected

Ki, Sae Hwi Korean Society of Plastic and Reconstructive Surge 2012 Archives of Plastic Surgery Vol.39 No.4

Early surgical correction of microstomia following Stevens-Johnson syndrome

Ki, Sae Hwi,Jo, Gang Yeon,Ma, Sung Hwan,Choi, Matthew Seung Suk Korean Cleft Palate-Craniofacial Association 2020 Archives of Craniofacial Surgery Vol.21 No.2

Stevens-Johnson syndrome (SJS) is a rare disease in which extensive toxic epidermolysis occurs after medication. Skin and mucous membranes are involved in about 90% of SJS cases, and webbing of mouth corners (microstomia) may occur when they are affected. Few reports have been issued on microstomia in SJS, and no consensus has been reached regarding treatment methods, timings, or results. We encountered a case of microstomia following SJS after ofloxacin medication in a 22-year-old woman treated by commissuroplasty using a lozenge-shaped excision. We present an appropriate correction method and surgical timing for microstomia following SJS.