Comparison of Proliferative Activity in Each Histological Subtypes of Benign and Atypical Intracranial Meningiomas by PCNA and Ki-67 Immunolabeling

최승진,장은덕,권성오,계대곤,박춘근,이상원,강준기,Choi, Seung Jin,Chang, Eun Deok,Kwon, Seung Oh,Kye, Dae Kon,Park, Choon Keun,Lee, Sang Won,Kang, Joon Ki The Korean Neurosurgical Society 2000 Journal of Korean neurosurgical society Vol.29 No.9

목 적 : 양성 뇌수막종에 비하여 이형성 및 악성 뇌수막종이 나쁜 임상적인 예후 및 양상을 보이는 것은 잘 알려져 있으나, 양성 뇌수막종에 있어서 각각의 병리조직학적 아형에 따른 생물학적 양상의 차이에 대해서는 잘 알려지지 않거나 일부 논란이 되고있다. 본 연구에서는 이형성 뇌수막종 및 양성 뇌수막종의 각각의 병리조직학적 아형에 따른 증식능의 차이여부를 알아보고자 PCNA와 Ki-67표지지수를 분석하였다. 방 법 : 본원에서 뇌수막종으로 수술을 시행하여 얻은, 재발을 보여 재수술을 시행한 2례를 포함하여, 파라핀에 포매시킨 27개의 조직을 대상으로 병리학적인 증식능을 분석하기 위해, PCNA에 대한 단일항체 및 MIB-1 단일항체를 이용한 면역조직화학적 염색을 시행하였다. 조직학적 분류상 meningothelial type이 8례, transitional type이 9례, fibroblastic type이 5례였으며, 이형성 수막종이 5례였다. 결 과 : PCNA표지지수의 평균값은 양성 수막종에서 meningothelial type이 $4.82{\pm}5.10%$, transitional type이 $9.01{\pm}4.25%$, fibroblastic type이 $5.66{\pm}5.32%$를 보였으나 이형성 수막종에서는 $27.62{\pm}19.67%$의 높은 지수를 나타냈고, Ki-67 표지지수의 평균값은 양성 수막종의 아형에서 각각 $0.43{\pm}0.85%$, $0.44{\pm}1.08%$, $0.24{\pm}0.18%$를 보이고, 역시 이형성 수막종에서는 $0.84{\pm}0.59%$의 높은 지수를 보였다. 즉, 양성 수막종에서 각각의 아형에 따른 PCNA 및 Ki-67 표지지수는 통계학적으로 의미있는 차이는 없었으나(p>0.05), 이형성 수막종에서는 의미있는 높은 표지지수를 보여(p<0.05) 양성 수막종에서 보다 높은 증식능을 보임을 알 수 있었다. 결 론 : PCNA 및 Ki-67 표지지수를 이용한 증식능의 비교결과, 양성 뇌수막종에서는 각각의 아형에 따른 생물학적 양상이나 예후는 차이가 없을것으로 생각되나, 이형성 수막종에서는 높은 증식능을 보여 이에 대한 예후를 예상할 수 있을것으로 생각되며, 또한 이러한 표지지수가 병리조직학적으로 양성과 이형성의 감별에 많은 도움이 될것으로 사료된다. Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

음곡 상응부위에 시술한 방기 약침이 LPS로 유도된 흰쥐의 염증성 신손상에 미치는 영향

김기춘 ( Ki Choon Kim ),임윤경 ( Yun Kyoung Yim ) 경락경혈학회 2013 Korean Journal of Acupuncture Vol.30 No.4

Objectives: This study aimed to evaluate the effects of Stephaniae tetrandrae Radix Herbal-acupuncture (SR-HA) at KI10 (Umgok) on nephritis induced by lipopolysaccharide (LPS) in rats. Methods: Male SD rats (n=32) were allocated to four groups; normal, LPS, saline, and SR-HA. The rats in the LPS, saline, and SR-HA groups were injected intra-peritoneally with LPS (2 mg/kg) to induce nephritis. The rats in the saline and SR-HA groups were treated with saline injection and SR-HA respectively at KI10 three times a week. To evaluate the effects of SR-HA at KI10 on nephritis in rats, the authors evaluated the numbers of WBC and neutrophils in blood, and the levels of creatinine, TNF-α, CINC-1 in serum, creatinine, total protein in urine and renal MPO. Results: SR-HA at KI10 significantly inhibited the increase of WBC and neutrophils in blood, TNF-α, CINC-1 in serum, creatine, total protein in urine and MPO in kidney of LPS-stimulated rats. Conclusions: SR-HA at KI10 may have therapeutic effects on nephritis in LPS-stimulated rats.

한국 근대 자유시의 미적 전략과 근대적 서정의 형식

노춘기(Noh, Choon-Ki) 한국시학회 2015 한국시학연구 Vol.- No.44

The purpose of this article is investigation of the formation and the esthetic attitude of Korean modern free-verse. In 1900’s the poem written in Korean language was recognized as an effective means of enlightenment campaign. But in the respect of subject consciousness and linguistic form, the writers of free-verse showed a different intention point from the senior writers. Since the emergence of the magazine of HakJiKwang, the younger writers presented the pioneering realization of the modern literature and the artistic expression. The modern free-verse in Korean appeared for the first time in the middle of 1910’s. And Hwang, Seok-Woo declared the aesthetic theory of the free-verse as a form of modern poetry in late 1910’s. He insisted that the starting point of Korean modern poetry must be in the free-verse. It meant that the free-verse is the essence of modern poetry. And he stipulated that the word of soul-nonusual and unpopular expression-is the essentials of poetic language. He denied the form of presentation of the previous pattern of poetry as a chantable lyrics. The writers of the freeverse advocated the realistic portrayal of the torturous world and the wounded subject. They defended the modern free-verse and suggested the aesthetic goal for the future of Korean modern poetry.

만성 호중구성 백혈병 1례

기장도,박치영,류보열,김태종,모성환,곽재정,문철호,박근홍,박유환,정춘해 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

Chronic neutrophilic leukemia is a very rare myeloproliferative disorder which is characterized by severe sustained mature neutrophilic leukocytosis in peripheral blood (PB), hepatosplenomegaly, elevated leukocyte alkaline phosphatase, serum uric acid. serun viatmin B 12 and the bleeding tendency despite normal platelet count and coagulation time, absence of philadelphia chromosome, and the absence of fever or underlying infection or disease sufficient to mimic a leukemoid reaction. We have experienced a 34-years-old male patient with atypical chronic neutrophilic leukemia. On admission, the leukocyte count was 56,860/uL. severe neutrophic leukocytosis with 76% neutrophils and rare immature forms on PB smear. Many neutrophils had toxic granules, and vacuoloes. Leukocyte alkaline phosphatase score was decreased. The cytogenetic study showed Philadelphia chromosome negative with normal karyotype.

<三韓拾遺>의 豫備的 考察

李春基 한양대학교 한국학연구소 1992 韓國學論集 Vol.20 No.-

This paper is only a part of the general study of the novel 'Samhan Seup-yu'(三韓拾遺). The purpose of this paper is to examine the original texts of the novel, current study on the novel, the family lineage and personal life of the author Kim So-haeng(金紹行) and the motive and process of his writing the book. Study on these subjects is believed to be of great help to understanding the novel 'Samhan Seup-yu' and its author. The conclusion of this study are as follows. Firstly, so far there has not been enough study on 'Samhan seup-yu'. There should be more study on the novel. Secondly, the extant texts of the novel can be classified into there at large; Seoul National University library original, Kora University library original and Karam Library original edition. But there are not any noticeable differance between them. Thirdly, the autheor Kim so-haeng was born in the influential Ahandong Kim family in the Choson Dynasty. but as he was a illegitimate child, he was restrained from getting any position in the government. So he devoted himself to learning and writting as a scholar and writer all his life. Lastly, the motive of his writing 'Samhan Seup-yu' can be traced back to his determined historical view and self-compensation phychology for his limited social status.

성화의 교육적 가능성과 수단에 관한 연구

한춘기 總神大學校 1998 總神大論叢 Vol.17 No.-

여고생의 건강상태와 체력·성적에 관한 연구

민춘기,김동건 충남대학교 자연과학연구소 1987 忠南科學硏究誌 Vol.14 No.1

In the present study we employed the CMI to analyse the health status data for the 111 students from the C Girls High School in Daejeon City. The following results have been obtained as regards body strength and academic scores of the students in each group on mental health. 1. Of the students tested, 61.3% were classified into the group Ⅰand Ⅱ(normal and near normal respectively) and the remaining 38.7% into the group Ⅲ and Ⅳ(near neurosis and neurosis respectively). 2. The differences in body strength and academic scores of the students among the various groups were not significant.

Voxel머리팬텀 제작 및 붕소중성자포획요법 선량계산에의 응용

이춘식,이춘익,이재기 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.2

해부학적으로 단순한 수학적인형팬텀의 한계를 극복하기 위한 voxel머리팬텀을 제작하고 BNCT(Boron Neutron Capture Therapy) 시행 시 선량분포를 계산하였다. 일반목적 몬테칼로 코드인 MCNP4B의 반복구조 알고리즘을 이용하여 voxel몬테칼로 계산체계를 수립하였고 두 가지 물질로 구성된 예시적 voxel팬텀과 기하체조합팬텀의 계산값 비교를 통해 계산체계를 검증하였다. 미국 NLM(National Library of Medicine)에서 제공하는 VHP man 인체단층사진에 대한 분할 및 색인작업을 통해 voxel머리팬텀을 제작하여 AP 및 PA 방향에서 입사하는 넓고 평행한 광자 및 중성자빔에 대한 선량값을 MIRD팬텀의 계산값과 비교한 결과 중성자빔 AP방향조사 시 MIRD팬텀에서는 볼 수 없는 안구로 인한 중성자 감쇠현상을 확인할 수 있었다. 3차원 정밀계산이 필요한 BNCT 시술시 선량분포계산을 위해 뇌 중앙에 직경 5cm의 구형 뇌종양 체적을 정의하고 뇌와 종양의 붕소 함량을 조정하여 10keV 및 40keV 상부입사 중성자에 의한 장기별 흡수선량을 계산한 결과 종양에 30㎍/g, 정상세포에 3㎍/g 의 붕소를 주입한 경우 붕소함량이 없을 때에 비해 2배 가량 큰 선량을 보였다. 본 연구를 통해 voxel몬테칼로기법을 이용한 선량평가체계를 수립하였고 정밀한 선량계산을 필요로 하는 치료방사선분야 선량계산에 실제 인체에 가까운 voxel팬텀의 응용가능성을 제시하였다. Voxel head phantom for overcoming the limitation of mathematical phantom in depicting anatomical details was constructed and example dose calculation for BNCT was performed. The repeated structure algorithm of the general purpose Monte Carlo code, MCNP4B was applied for voxel Monte Carlo calculation. Simple binary voxel phantom and combinatorial geometry phantom composed of two materials were constructed for validating the voxel Monte Carlo calculation system. The tomographic images of VHP man provided by NLM(National Library of Medicine) were segmented and indexed to construct voxel head phantom. Comparison of doses for broad parallel gamma and neutron beams in AP and PA directions showed decrease of brain dose due to the attenuation of neutron in eye balls in case of voxel head phantom. The spherical tumor volume with diameter, 5cm was defined in the center of brain for BNCT dose calculation for downward neutron beam of 10keV and 40keV, the tumor dose is about doubled when boron concentration ratio between the tumor to the normal tissue is 30㎍/g to 3㎍/g. This study established the voxel Monte Carlo calculation system and suggested the feasibility of precise dose calculation in therapeutic radiology.

컴퓨터 바둑에서 상태재현 회피에 의한 탐색공간 간소화 기법

夫起東,李도漢,權六春,朴鉉秀 慶一大學校 1997 論文集 Vol.14 No.2

In this paper, we implement a methodology that searches a complete game tree to solve the problem caused by inaccuracy of a heuristic function used in the MIN/MAX algorithm. The methodology is based on the idea searching all possible paths from one root to all terminal nodes. The devised pruning method is constructed by noticing the fact that the frequent reappearances of the same status are occured in various sequences during the processing of the Life and Death problem of Go. When this algorithm detects reappearance of the same status, it links the child of current node with the already examined one. Consequently searching cost can be reduced by the linking process. Finally, generated solution tree is expanded to acyclic directed graph by the sharing method. Experimental result shows that the proposed methodology reduces the temporal and spatial traversing cost of the AND/OR game tree. And we apply the methodology to the status evaluating problem, a major problem of Go, because the problem must reflects the clear estimation of strings status on a board.

高校運動選手와 一般學生의 性格特性에 關한 比較 分析

閔春基,金昌林 忠南大學校體育科學硏究所 1986 體育科學硏究誌 Vol.4 No.1

1. The personality characteristics of general students and athletes were compared. The number of athletes compared to that of general students was more than 50 % on the national standardization examination of personality diagnosis. 2. The personality characteristics by sports events In a 5091 level on the national standardization examination of personality diagnosis. The order was Track & field > Judo > Roller Skate >Handball > Boxing, Weight lifting, Shooting, Gymnastics, Tae Kwon Do > Archery. In the five clinical scales, the order was Handball > Wrestling, Roller Skate, Tae Kwon Do >Shooting, Archery > Gymnastics. 3. The personality characteristics by athletes motivation for pursuing sports. In a 50% level on the national standardization examination of personality diagnosis. The order was suggestion by the family> suggestion by teachers >person desire to pursue athletics. 4. The personality characteristics by athletes career. In a 50 % level on the national standardization examination of personality diagnosis. The order was more than six years >from four years to six years > from two years to four years. 5. The personality characteristics by practice time. In a 50% level on the national standardization examination of personality diagnosis. The order was from four hours to five hours > from four hours to four hours >to three hours more than five hours. 6. The personality characteristics by the birthplace. In a 50 % level on the national standardization examination of personality diagnosis. The order was middle and small city >farming and fishing village > mountain village. 7. The personality characteristics by the roles of athletes. In a 50% level on the national standardization examination of personality diagnosis. The order was main >candidate. 8. The personality characteristics by the score of athletics. In a 50% level on the national standardization examination of personality diagnosis, The order was winning a price at a national athletic meet. > Winning a prize at a provincial athletic meet. >winning no prize. 9. The personality characteristics by the experience of losing athletics. In a 50% on the national standardization examination of personality diagnosis. The order was from five times to nine times more than thirteen ti meta from nine times to thirteen times to five times.