Comparison of Hanwoo with Holstein of OPU derived Embryo and Offspring Production

Jong‐In Jin,Byung‐Hyun Choi,Seong‐Su Kim,Hyun‐Tae Jo,Il‐Keun Kong 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

The objective of this study was to evaluate the comparison of production efficiency of oocytes and OPU (ovum pick‐up) derived embryos of Hanwoo with Holstein. The OPU session of each species (6 cows) was carried out from the Hanwoo (106 sessions) and Holstein (114 sessions) at intervals of 3 4 days (2 times per week) for 3 months. Cumulus‐oocyte‐complexes (COCs) retrieved were classified into 4 grades by the status of oocyte cytoplasm and cumulus cells. The COCs collected were matured in vitro in TCM‐199 supplemented with 10% FBS, 10 mg/ml FSH and 1 mg/ml estradiol‐ 17β in 5% CO2 and over 99% humidity for 24 h. After 24 h co‐incubation with post‐thaw sperm, the presumed zygotes were cultured in CR1aa medium with 4 mg/ml BSA for 3 days and then changed CR1aa medium with 10% of FBS for another 3 4 days. The Mean number of aspirated follicles and collected oocytes were not significantly different between Hanwoo and Holstein spacies (10.4±0.42 vs. 11.4±0.41 and 7.5±0.38 vs. 6.1±0.37 per session). But the collection rate of oocytes from aspirated follicles were significantly higher in Hanwoo (72.8%) than that in Holstein (53.6%) (p< 0.05). Furthermore, the average number of good quality oocytes (Grade I and II) was 5.9±0.28 and 4.1±0.27 (Mean±SD), and the cleavage rate and the development rate to blastocysts was significantly (p<0.05) higher in Hanwoo (40.0%) than Holstein (21.6%). The OPU derived embryos of Hanwoo were transferred 83 times into 52 recipients and then 42 calves were produced from 44 pregnancy recipients. In conclusion, the efficiency of OPU derived embryo was significantly different between Hanwoo and Holstein species. In vitro culture system for OPU derived embryo production should be optimized for industrialization and the improvement of livestock.

VDT작업별 정신사회적 스트레스와 근골격계 장애에 관한 연구

백남종,강종두,주영수,배인근,권호장,박종만,조수헌,김돈규,김재용,최홍렬 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4

It has been hypothesized that jobs that have both high psychological demands and low decision latitude("job strain") can lead to musculoskeletal disorder. The objective of this study was to test whether job strain was correlated with the presence of work-related musculoskeletal disorder, especially myofascial pain syndrome(MPS). Information on demographic factors, confounders such as household load and taking care of children or not, and scores for decision latitude, job demand, and social support was obtained by self-administered questionnaire, which had been developed in Korean language, by adopting NIOSH instrument and Extended Karasek Model(16 items). All subjects were also examined by rehabilitation medicine specialists for musculoskeletal disorders. Subjects(n=370) could be categorized into 4 groups, these were, housewives(n=89), shipyard CAD workers(n=89), general female workers(n=79; nurses, insurance counselors, public officials, clerks, etc), and telephone directory assistance operators(n=113). Results from univariate analyses indicated that all demographic factors, all confounders, scores for decision latitude and social support were not associated with the risk of musculoskeletal disorder. However, score for job demand was higher in musculoskeletal disorder cases than others. In subgroup analysis, this association was convinced again, in telephone directory assistance operators. Job strain model showed that the group of telephone directory assistance operators was high-stain group, and OR of musculo skeletal disorder was 2.446(95% C. I. : 1.174, 5.096), when comparing this with the low strain group. In conclusion, job strain is a risk factor for work-related musculoskeletal disorder.

[논문]연약지반의 지반침하 계측 사례

임종철,장지건 釜山大學校生産技術硏究所 2004 生産技術硏究所論文集 Vol.63 No.-

연약지반의 지반침하 계측 사례

[논문]SCP 설치지반의 압밀침하특성에 관한 실험적연구

임종절,김동호,박이근 釜山大學校生産技術硏究所 2004 生産技術硏究所論文集 Vol.63 No.-

바야흐로 개방화 시대를 맞이하여 세계 각국들과 교역량이 늘어나면서 여러 제반시설을 필요로 하게 되었다. 특히 항만과 같은 대규모 공사의 기초 작업으로 먼지 해야할 일은 시공 예정지역에 형성되어있는 해양의 연약점성토 지반을 개량해서 강도가 높은 안정된 기초를 가지게 하는 것이 중요하다. 토압, 지진, 파압 둥에 우수함이 이미 일본 등 여러 나라에서 검증되어 온 SCP공법은 우리의 지반조건에 맞는 설계자료가 필요하다. 이를 위해 본 연구에서는 SCP 압밀실험에서 치환율에 따라서 압축지수의 변화관계를 분석하였다.

[논문]연약지반 성토시의 면적비를 활용한 안정관리기법

임종철,엄진현,박이근 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

국제 무역시대의 도래로 항만 물동량이 크게 증가함에 따라 항만시설의 확충이 시급한 실정이다. 비교적 양호한 지반에서의 항만공사는 종래에 대부분 행해졌으며, 현재는 아주 연약한 지반에서의 해안매립을 통한 부지조성 등의 대규모 공사가 진행중이다. 연약지반 성토시 하부지반은 압밀침하와 전단변형이 동시에 발생하므로, 양자를 고려한 안정성 판단이 매우 중요하다. 본 연구에서는 현장에서 파괴가 발생한 부산 신항만공사의 서방파제 계측결과를 분석하고 수치해석을 수행하여, 면적비 안정관리기법을 제안하였다.

[논문]성토체의 압축량 산정방법에 관한 실험적 연구

임종절,박이근,Ho-Seong Ko 釜山大學校生産技術硏究所 2004 生産技術硏究所論文集 Vol.63 No.-

현장에서 시공시 성토량은 성토체 자체의 압축침하(즉시 및 크리프)와 시공중 및 시공완료 후 발생한 제체하부원지반의 압밀침하, 암버럭 사이의 간극채룹에 의한 토량, 강우시 토사유출에 의한 손실토량 등에 의해 압축량은 영향을 받는다. 이러한 여러 가지 이유로 성토할 흙의 종류에 따른 토량환산계수의 허점을 보완하고자 소정 밀도로 다짐 성토공시체에 일정재하하중을 정적으로 가하여 성토체 압축침하량을 산정하는 식이 있지만 상수값 산정이 다소 불명확하게 표현된다. 본 연구에서는 성토체의 다짐도와 건조밀도를 달리하여 응력 단계별로 즉시변형률과 크리프변형률을 산정하여 자중에 의한 압축량 산정방법을 제안하고자 한다.

남성유방에 발생한 지방육종

최종범,임성철,기근홍,전호종,서재홍 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.3

HPV에 감염된 자궁경부 상피세포의 Keratin 아형 변화에 관한 연구

기근홍,이영미,장원재,임용,임성철,김창원,전호종 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The human papillomavirus (HPV) are wide spread in the world, causing proliferation of epidermal and mucosal surface. Certain papillomaviral types are oncogenic in vivo and in vitro. HPV DNA has been detected in most of cervical lesions such as condyloma acuminata, dysplasias, and invasive carcinomas. The materials for this study consisted of 5 dysplasias. 5 squamous cell carcinomas, and 5 exocervix for control, that resected for definite treatment of uterine disease at Chosen University Hospital from January, 1991 to December, 1993. All cases were performed of in situ hybridization for HPV DNA type 6/11, 16, and 18. And also immununohistochemical stain for panel of monoclonal cytokeratin (CK) antibodies were done. The result obtained is as fallows : 1. HPV DNA type 6/11 are detected in all dysplasias. HPV DNA type 16 and 18 are detected in squamous cell carcinomas. 2. Stratifed squamous epithelium of exocervix are positive staining for CK 1, CK 5/6, CK 8, CK 13, CK 14, and CK 19. HPV DNA type 6/11 infected cells are positive for CK 1, CK 13, CK 14, CK 17, CK 18, and CK 19. HPV DNA type 16/18 infected cells are positive for CK 1, CK 5/6, CK 10, CK 13, CK 14, CK 17, CK 18, and CK 19. In conclusion, HPV infection of uterine cervix is associated with change of normal cytokeratin expression pattern. Dysplastic cells are changed of CK 5/6, CK 8, CK 17 and CK18. Squamous cell carcinoma cells are changed of CK 8, CK 17, and CK 18.

원광대학교병원 응급실에 내원한 치과 응급 환자에 대한 임상적 연구

이동근,민승기,양찬영,문철,김종구 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

This is a retrospective clinical study on 3,243 patients who had visited the Emergency Room of Wonkwang University Hospital and then been treated in the Department of Oral and Maxillofacial Surgery during recent 5 years from Jan. 1, 1996 to Dec. 31, 2000. The obtained results were as follows : The total number of patients was 3,243 and the ratio of sex was 1.86:1. The highest monthly incidence was shown in October and March to 19%, and the age distribution peaks was the 3rd decade(24.7%), followed by the 1st(21.7%) and the 4th decade(15.7%). The percentage of admission were 4.8%. The frequent cause of admission were facial bone fracture(70%), infection(16%), soft tissue injury(14%). Trauma(70.9%) was the most frequent cause in dental emergency patients, odontogenic infection(24.3%), oral hemorrhage and TMJ dislocation were next in order of frequency. About the cause for trauma, traffic accident was the most, and fall down, slip down injury and assault were followed. Soft tissue injury group(56%) was the most prevalent, followed by tooth injury group(27%) and facial bone injury group(17%). In facial bone injury group, the mandibular fracture(55%) showed the highest incidence followed by zygomaticomaxillary complex fracture, nasal bone fracture, maxilla fracture, and zygomatic arch fracture. In the mandibular fracture, symphysis(40%) was the most common location followed by condyle(29.4%), angle(22.1%), and body area(8.5%). Tooth fracture showed the highest incidence as 35% followed by subluxation(29%), avulsion(20%), and concussion(16%). In infection patients group, the ratio of admission was 5.8% and the major visiting cause was acute pulpitis(32.3%). The major treatment method was the incision and drainage(30%) followed by endodontic treatment(28%), curettage(22%), extraction(7.8%), and so forth. In TMJ dislocation group, the highest incidence age was 3rd decade and 4th decade(51%). In post-operative bleeding group, the major cause of bleeding was non-fulfillment of doctor's instruction and bleeding was almost stoped by only gauze biting.

두부손상 환자에서 정신증상과 뇌 손상부위와의 관계 : 우울증상과 인지기능 장애를 중심으로 Focused on Depressive Symptoms and Cognitive Dysfunctions

황익근,김종상,정상근 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.6

To investigate the relationship between psychiatric symptoms and brain lesion site, thirty eight psychiatric inpatients with history of head trauma were selected. Patients were divided into three groups according to lesion sites ; patients with left hemispheric lesion (N=13), patients with right hemispheric lesion(N=11), and patients with ill defined lesion(N=14). Psychiatric symptoms were examined focused on depression and cognitive functions. Depressive symptoms were checked by Hamilton Rating Scale for Depression(HRSD), and cognitive functions were checked by Mini-Mental Status Examination(MMSE) and Korean Wechsler Intelligence Scale(KWIS). The results were as follows : 1) Patients with left hemispheric lesion, especially with left anterior lesion, showed significantly higher HRSD score than patients with another lesion sites(p<0.05). 2) Moderate to severe depression, whose HRSD score was higher than 18, was more common in patients with left hemispheric lesion than in patients with another lesion sites. 3) MMSE scores and KWIS scores of all three groups were not significantly different each other. 4) ‘Calculation’scores were lower in patients with left hemispheric lesion than in patients with another lesion sites. 5) ‘Language’scores were significantly lower in patients with left hemispheric lesion than in patients with another lesion sites(p<0.0.5).