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Jae‐Il Bang,A‐Na Ha,A‐Ra Cho,Kyeong‐Lim Lee,MD. Fakruzzaman,Il‐Keun Kong 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
The present research was carried out to evaluate the possibility of female offspring production using artificial insemination buffer (AIB) before artificial insemination (AI). To do it, we carried out the optimization of AIB, making of AIB gun and analysis of affecting AI rate after AIB treatment. AIB made with the base of Tris‐buffer supplemented with L‐arginine and several materials that could be reduced the motility of male sperm compared with female one. This mean that female sperm could be increased the possibility of fertilization with ovum compared with male one. AIB must be deposited into 2nd to 4th cervix by the guide of AIB gun. After 15 min of AIB insertion, frozen semen was deposited into same place after. Total 352 cattle were inseminated with AIB insemination and was not significant difference between AIB and traditional AI rate (56.8 vs. 55.7%). However, AIB AI rate was significantly differs among 12 different farms. The parturition number of cows did not effect on AIB AI rate among 1st to 7th parturition number of cows. The proportion of AIB AI success rates in hanwoo cows was significantly higher than in dairy cows (61.0% vs. 48.7%), but the average AI success rate was not different between AIB and conventional AI (56.8% vs. 55.7%). The female offspring production rate in 2nd to 4th cervix deposition place was significantly higher than in uterus body (77.7% vs. 59.6%, p<0.05). The injection volume of AIB in 5 and 10 ml was significantly higher than in 2 ml (77.7, 78.7 vs. 51.8%, p<0.05), but not different between 5 and 10 ml ABI volume. The best exposure time of AIB in the cervix was 10 and 15 min rather than that of 5 min (79.2%, 77.2% vs. 63.2%, p< 0.05), and so AIB have to expose at least 10 min to get higher female offspring. In conclusion, AIB could be used in AI industry to produce female offspring and also AIB AI can be increased the AI success rate compared with traditional AI rate.
방역작업자의 브롬화메틸(methyl bromide) 노출수준과 생물학적 모니터링
이종성,이용학,신재훈,최정근,오차재,정호근 한국산업위생학회 2001 한국산업보건학회지 Vol.11 No.3
This study was performed to estimate environmental and biological monitoring of worker exposed to methyl bromide through quarantine fumigation. Airborne methyl bromide and it's metabolites were analyzed by gas chromatography and ion chromatography, respectively. The results are as followings; airborne concentration of methyl bromide(TWA) was 2.08±1.56 ppm(N=8). Dispersion and setting/degas groups were 0.67±0.12 ppm(N=2) and 2.54±1.53ppm(N=6), respectively. Bromide ion concentration in serum was 23.40±14.91㎎/ℓ(N=10) in the exposed workers and 4.74±0.82㎎/ℓ(N=21) in the non-exposed workers. Bromide ion concentration in urine was 35.56±26.89㎎/ℓ(N=11) in exposed group and 6.62±2.31㎎/ℓ(N=21) in non-exposed group. Good correlation was observed between concentration of serum and urine(r^2=0.890 p<0.01). No significant correlations of other determinanats were observed. Calcuated from a regressive curve. the biological half lifes of serum and urine were 10.7 and 5.9 days. In these results, biological monitoring of bromide ion of serum and urine provided useful information for evaluating exposure of workers to methyl bromide, so that an availability of bromide ion of biological samples was showed as biological monitoring indices for methyl bromide.
정상근,최재호 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.1
이상과 같이 임신 및 수유기간, 여성내분비 기능과 관련된 정신과 사용 약물들의 부작용과 일반적인 원칙 또는 관리방법 등에 대해 알아보았다. 무엇보다도 중요한 것은 개개인의 특정한 정신과적 질환의 약물치료와 여성 특유의 특정 상황에 대한 이해득실을 신중하게, 포괄적으로 평가하는 것이다. 정신과 의사들은 이러한 내용들을 충분히 숙지함으로서, 임상 실제 상황에서 임신, 수유, 여성내분비 기능과 관련된 부작용을 최소화하고 예방할 수 있다. The management of psychiatric problems during pregnancy and lactation represents a unique and complex clinical situation. When women take psychotropic medications in these conditions, they and their fetus or neonate may experience unwanted, adverse effects due to these medications. Inthe 1st trimester, teratogenicity is the main drug risk, in the 2-3rd trimester, growth retardation and neurological damage may occur and after birth, drug withdrawal effects may occur. Women may also experience sexual dysfunction or amenorrhea. Therefore, under the these background we reviewed adverse effects, malformations during pregnancy and lactation and female endocrine dysfunction. We also reviewed the principles and management of those situations.
Bacillus lentimorbus WJ5의 감마선유도 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자의 microarray 분석
이영근,장화형,장유신,허재호,형석원,정혜영 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.3
본 연구에서는 감마선으로 유도된 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자들의 발현을 연구하기 위하여, B. lentimorbus WJ5의 방사선 유도 돌연변이체에서 발현되는 유전자를 DNA microarray로 동시에 탐색하였다. DNA microarray는 B. lentimorbus WJ5 genome을 무작위로 절단하여 2,000단편으로 구성하였으며, 감마선 (^(60)Co)으로 유도된 7돌연변이체의 발현을 정량적으로 관찰하였다. 클러스터 분석결과 발현된 408 유전자 중 27개가 감마선 유도 돌연변이체 모두에서 유의하게 발현이 증가되었다. 특히, 복구(mutL, mutM)에너지 대사 (acsA, sdhB, p하, yhjB, citB), protease(npr), 산화자극에 대한 환원 (HMM)관련 유전자들이 동시에 증가되었다. 이는 감마선 유도 돌연변이체들에서 자발적인 직/간접 복구 관련 유전자의 발현 증가는 방사선 노출 직후 보이는 stress response와는 다른 현상임을 나타내는 것으로 생각된다. To study the radiation related gene expression in mutants of Bacillus lentimorbus WJ5 induced by gamm radiation, the simultaneous gene expression was analyzed by DNA microarray. We constructed DNA chips including two thousand randomly digested genome spots of B. lentimorbus WJ5 and compared its quantitative aspect with seven mutants induced by gamma radiation (^(60)Co). From the cluster analysis of gene expression pattern, totally 408 genes were expressed and 27 genes were significantly upregulated by the gamma radiation in all mutants. Especially, genes involved in repair (mutL, mutM), energy metabolism (acsA, sdhB, pgk, yhjB, citB), protease (npr), and reduction response to oxidative stress (HMM) were simultaneously upregulated. It seems that the induction of the direct and/or indirect repair related genes in mutants induced by gamma radiation could be remarkably different from the adaptive responses against acute exposure to radiation.