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Lee-ching Lew,Sy-bing Choi,Boon-yin Khoo,Sasidharan Sreenivasan,Kee-leong Ong,Min-tze Liong 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.2
Hypercholesterolemia is one of the primary risk factors for cardiovascular diseases. The use of lactobacilli probiotics to reduce blood cholesterol levels have been extensively reported. However, more information is needed to evaluate the possible mechanisms involved and to identify possible targets for further therapeutic development. In this study, strains of lactobacilli were screened based on the ability to assimilate cholesterol, and prevention of cholesterol accumulation in hepatic (HepG2) and intestinal (HT-29) cells. Cell free supernatant (CFS) from Lactobacillus plantarum DR7 showed a higher ability to assimilate cholesterol, reduction in cholesterol accumulation in both HepG2 and HT-29 cells, accompanied by reduced mRNA expression of HMG-CoA reductase (HMGCR) in HepG2 (p<0.05), compared to other lactobacilli. The reduction of HMGCR expression was also diminished in the presence of an AMPK inhibitor (Compound C), suggesting that L. plantarum DR7 exerted its effect via the AMPK pathway, typically via the phosphorylation of AMPK instead of the AMPK mRNA expression in HepG2 (p<0.05). Altogether, our present study illustrated that lactobacilli could exert cholesterol lowering properties along the AMPK pathway, specifically via phosphorylation of AMPK that led to reduced expression of HMGCR.
임정은,Ching Hanna,Yoon Jeong-Kee,전누리,Kim YongTae 나노기술연구협의회 2021 Nano Convergence Vol.8 No.12
Recent developments of organoids engineering and organ-on-a-chip microfluidic technologies have enabled the recapitulation of the major functions and architectures of microscale human tissue, including tumor pathophysiology. Nevertheless, there remain challenges in recapitulating the complexity and heterogeneity of tumor microenvironment. The integration of these engineering technologies suggests a potential strategy to overcome the limitations in reconstituting the perfusable microvascular system of large-scale tumors conserving their key functional features. Here, we review the recent progress of in vitro tumor-on-a-chip microfluidic technologies, focusing on the reconstruction of microvascularized organoid models to suggest a better platform for personalized cancer medicine.
Chien-Wei Hou,Shun-Yu Chang,Kee-Ching Jeng 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.4
Amyloid beta-protein (Ab) is involved in thepathogenesis of Alzheimer’s disease (AD). Ab induces freeradical production in neuronal cells, leading to oxidativestress and up-regulation of c-Jun N-terminal kinases (JNK),extracellular-signal-regulated kinases (ERK), p38 mitogenactivatedprotein kinase (MAPK) pathways and pro-apoptoticBax expression. Sesamin has been shown to haveprotection to several models of neurodegenerative diseasesby its antioxidant and anti-inflammatory properties. In thepresent study, we examined the neuroprotective effect of asesamin derivative, 3-bis (3-methoxybenzyl) butane-1,4-diol (BBD) on Ab1–42 induced cytotoxicity of PC12 cells. Ab1–42 induced lipid peroxidation, calcium, reactive oxygenspecies from the PC12 cells. The effect of BBD onthese harmful factors and the related signaling pathwayswere examined by biochemical and western blot assays. The result showed that BBD protected PC12 cells fromAb1–42 induced cytotoxicity with the increased cell viabilityand acetylcholine release, and the decreased lactatedehydrogenase, malondialdehyde and calcium release. BBD significantly reduced Ab-induced JNK, ERK, p38MAPK pathways and Bax expression in PC12 cells. Therefore the neuroprotective effect of BBD on Abinducedcytotoxicity was involved with antioxidant andanti-inflammatory effects. The result would help thedevelopment of new CNS drug for protection of AD.
Hock, Lim Kuang,Ghazali, Sumarni Mohamad,Cheong, Kee Chee,Kuay, Lim Kuang,Li, Lim Hui,Huey, Teh Chien,Ying, Chan Ying,Yen, Yeo Lay,Ching, Fiona Goh Swee,Yi, Khoo Yi,Lin, Chong Zhuo,Ibrahim, Normala,Mu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10
Intention to smoke is a valid and reliable factor for predicting future smoking habits among adolescents. This factor, however, has received inadequate attention in Malaysia. The present paper elaborates the prevalence and factors associated with intent to initiate or to cease smoking, among adolescent nonsmokers and smokers in Kota Tinggi, Johor, Malaysia. A total of 2,300 secondary school students aged 13-16 years were selected through a two-stage stratified sampling method. A set of standardized questionnaires was used to assess the smoking behavior among adolescents and the inter-personal and intra-personal factors associated with smoking intention (intention to initiate smoking or to cease smoking). Multivariable logistic regression was used to identify factors related to smoking intention. The prevalence of intention to smoke in the future or to cease smoking among non-smoking adolescents and current smokers were 10.7% and 61.7% respectively. Having friends who smoke, social influence, and poor knowledge about the ill effects on health due to smoking showed significant relationships with intention to smoke in the future among non-smokers. Conversely, perceived lower prevalence of smoking among peers, weak contributory social influence, and greater awareness of the ill effects of smoking are factors associated with the intention to cease smoking sometime in the future. The study found that prevalence of intention to initiate smoking is low among non-smokers while the majority of current smokers intended to cease smoking in the future. Existing anti-smoking programmes that integrate the factors that have been identified in the current study should be put in motion to reduce the prevalence of intention to initiate smoking and increase the intention to cease smoking among adolescents.
Bariatric Surgery in Kidney Transplant Candidates and Recipients: Experience at an Asian Center
Sarah Ying Tse Tan,Phong Ching Lee,Sonali Ganguly,Peng Chin Kek,Terence Kee,Quan Yao Ho,Sobhana Thangaraju 대한비만학회 2022 Journal of obesity & metabolic syndrome Vol.31 No.4
Background: Kidney transplant (KT) candidates and recipients with obesity experience more frequent complications such as infection, poorer allograft outcomes, diabetes, and mortality, limiting their eligibility for transplantation. Bariatric surgery (BS) is not commonly performed among KT patients given concerns about immunosuppression absorption, wound healing, infections, and graft outcomes. Its role has not been described before in an Asian KT patient setting. Methods: A retrospective review of patients who underwent BS at the largest KT center in Singapore from 2008 to 2020 was conducted. Metabolic outcomes, immunosuppression doses, graft outcomes, and mortality were studied. Results: Seven patients underwent BS and KT (4 underwent BS before KT, 3 underwent BS after KT; 4 underwent sleeve gastrectomy, 3 underwent gastric bypass). Mean total weight losses of 23.8% at 1 year and 18.6% at 5 years post-BS were achieved. Among the five patients with diabetes, glycemic control improved after BS. There were no deaths in the first 90 days or graft loss in the first year after KT and BS. Patients who underwent BS after KT had no significant changes in immunosuppression dose. Conclusion: BS can be safely performed in KT recipients and candidates and results in sustainable weight losses and improvements in metabolic comorbidities. Although no major complications were observed in our study, close monitoring of this complex group of patients is imperative.
Effect of Sesamin on Apoptosis and Cell Cycle Arrest in Human Breast Cancer MCF-7 Cells
Siao, An-Ci,Hou, Chien-Wei,Kao, Yung-Hsi,Jeng, Kee-Ching Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
Dietary prevention has been known to reduce breast cancer risk. Sesamin is one of the major components in sesame seeds and has been widely studied and proven to have anti-proliferation and anti-angiogenic effects on cancer cells. In this study, the influence of sesamin was tested in the human breast cancer MCF-7 cell line for cell viability (MTT assay) and cell cycling (flow cytometry). Results showed that sesamin dose-dependently (1, 10 and $50{\mu}M$) reduced the cell viability and increased LDH release and apoptosis (TUNEL assay). In addition, there was a significant increase of sub-G1 phase arrest in the cell cycle after sesamin treatment. Furthermore, sesamin increased the expression of apoptotic markers of Bax, caspase-3, and cell cycle control proteins, p53 and checkpoint kinase 2. Taken together, these results suggested that sesamin might be used as a dietary supplement f or prevention of breast cancer by modulating apoptotic signal pathways and inhibiting tumor cell growth.