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RISS 인기검색어
- 검색키워드
- 저자 : Kea-Joung (검색결과 3 건)
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金桂蓮,李京任,李貞淑 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-
Even though the weather resistance of fiber is a very important subject, there are not many term paper. If one places fibral wear in the weather of under similiar conditions ultraviolet light will cause lowering of the goods quality, expecially the strength and elongation factor will become lower and also results in decoloration. In this experiment using endurdence's most basic out-door method we are trying to estimate cotton, silk, rayon, nylon, acryl fiber's weather resistance in the sense of the change in strength. The results that we got were, 1. The fiber with the greatest resistance was acryl and least were silk. 2. Exhaust gas cuts off ultraviolet light and the result is decrease of fibers change. 3. The elongation percentage of cotton and rayon rise at first and than decrease as time go on.
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Transmission of Bovine β-Casein/Human Lactoferrin Fusion Gene in Transgenic Cattle
Yong-Mahn Han,Deog-Bon Koo,Jung-Sun Park,Young-Hun Kim,Kea-Joung Lee,Kyung-Kwang Lee 한국동물생명공학회(구 한국동물번식학회) 2005 Reproductive & developmental biology Vol.29 No.4
This study was conducted to test whether the transgenic cattle pass the transgene to their progeny through germ cells, and whether the transgene is expressed in the mammary gland of ransgenic cows. Two male ransgenic calves were born from IVF-derived embryos injected with bovine β-casein/human lactoferrin fusion gene and then grew up to be reproducible. Semen was collected from a transgenic bull after 18 mon of age and then frozen. Bovine oocytes matured in vitro were fertilized with spermatozoa of the transgenic bull and cultured in 50㎕ drops of CRlaa medium supplemented with 3 mg/mL BSA. After 48 h of culture, cleaved embryos were determined for the presence of transgenes by DNA polymerase chain reaction (PCR). Proportion of transgene positives among bovine embryos fertilized with sperm of the transgenic bull was 20.9% (28/134). One of transgenic bulls did not produce transgenic sperm. Out of 34 calves produced from recipient heifers inseminated with semen of the other bull, 3 (8.8%) were transgenic animals (2 females and 1 male). Thus, one transgenic bull showed a low transmission frequency below Mendelian levels in both the IVF-derived embryos and his progeny. It was demonstrated by Southern blot that copy numbers of the transgene in the transgenic progeny enhanced about 1.8 times as compared to those of the founder bull The results demonstrate that the transgenic bull carrying human lactoferrin gene could pass his transgene to the progeny through germ cells, although he is a germ-line mosaic.
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Transmission of Bovine $\beta-Casein/Human$ Lactoferrin Fusion Gene in Transgenic Cattle
Han Yong-Mahn,Koo Deog-Bon,Park Jung-Sun,Kim Young-Hun,Lee Kea-Joung,Lee Kyung-Kwang The Korean Society of Animal Reproduction 2005 Reproductive & developmental biology Vol.29 No.4
This study was conducted to test whether the transgenic cattle pass the transgene to their progeny through germ cells, and whether the transgene is expressed in the mammary gland of ransgenic cows. Two male ransgenic calves were born from IVF-derived embryos injected with bovine $\beta-casein/human$ lactoferrin fusion gene and then grew up to be reproducible. Semen was collected from a transgenic bull after 18 mon of age and then frozen. Bovine oocytes matured in vitro were fertilized with spermatozoa of the transgenic bull and cultured in $50\;{\mu}L$ drops of CRlaa medium supplemented with 3 mg/mL BSA. After 48 h of culture, cleaved embryos were determined for the presence of transgenes by DNA polymerase chain reaction (PCR). Proportion of transgene positives among bovine embryos fertilized with sperm of the transgenic bull was $20.9\%$ (28/134). One of transgenic bulls did not produce transgenic sperm. Out of 34 calves produced from recipient heifers inseminated with semen of the other bull, 3 $(8.8\%)$ were transgenic animals (2 females and 1 male). Thus, one transgenic bull showed a low transmission frequency below Mendelian levels in both the IVF-derived embryos and his progeny. It was demonstrated by Southern blot that copy numbers of the transgene in the transgenic progeny enhanced about 1.8 times as compared to those of the founder bull The results demonstrate that the transgenic bull carrying human lactoferrin gene could pass his transgene to the progeny through germ cells, although he is a germ-line mosaic.
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