http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kanaya, Y.,Irie, H.,Takashima, H.,Iwabuchi, H.,Akimoto, H.,Sudo, K.,Gu, M.,Chong, J.,Kim, Y. J.,Lee, H.,Li, A.,Si, F.,Xu, J.,Xie, P.-H.,Liu, W.-Q.,Dzhola, A.,Postylyakov, O.,Ivanov, V.,Grechko, E.,Ter Copernicus GmbH 2014 Atmospheric Chemistry and Physics Vol.14 No.15
<P>Abstract. We conducted long-term network observations using standardized Multi-Axis Differential optical absorption spectroscopy (MAX-DOAS) instruments in Russia and ASia (MADRAS) from 2007 onwards and made the first synthetic data analysis. At seven locations (Cape Hedo, Fukue and Yokosuka in Japan, Hefei in China, Gwangju in Korea, and Tomsk and Zvenigorod in Russia) with different levels of pollution, we obtained 80 927 retrievals of tropospheric NO2 vertical column density (TropoNO2VCD) and aerosol optical depth (AOD). In the technique, the optimal estimation of the TropoNO2VCD and its profile was performed using aerosol information derived from O4 absorbances simultaneously observed at 460-490 nm. This large data set was used to analyze NO2 climatology systematically, including temporal variations from the seasonal to the diurnal scale. The results were compared with Ozone Monitoring Instrument (OMI) satellite observations and global model simulations. Two NO2 retrievals of OMI satellite data (NASA ver. 2.1 and Dutch OMI NO2 (DOMINO) ver. 2.0) generally showed close correlations with those derived from MAX-DOAS observations, but had low biases of up to ~50%. The bias was distinct when NO2 was abundantly present near the surface and when the AOD was high, suggesting a possibility of incomplete accounting of NO2 near the surface under relatively high aerosol conditions for the satellite observations. Except for constant biases, the satellite observations showed nearly perfect seasonal agreement with MAX-DOAS observations, suggesting that the analysis of seasonal features of the satellite data were robust. Weekend reduction in the TropoNO2VCD found at Yokosuka and Gwangju was absent at Hefei, implying that the major sources had different weekly variation patterns. While the TropoNO2VCD generally decreased during the midday hours, it increased exceptionally at urban/suburban locations (Yokosuka, Gwangju, and Hefei) during winter. A global chemical transport model, MIROC-ESM-CHEM (Model for Interdisciplinary Research on Climate-Earth System Model-Chemistry), was validated for the first time with respect to background NO2 column densities during summer at Cape Hedo and Fukue in the clean marine atmosphere. </P>
Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif
Nguyen, T.N.,You, D.J.,Kanaya, E.,Koga, Y.,Kanaya, S. North-Holland Pub ; Elsevier Science Ltd 2013 FEBS letters Vol.587 No.9
The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the first aspartate residue, as in the active site of RNase H2. However, the structure and enzymatic properties of LC9-RNase H1 highly resemble those of RNase H1, instead of RNase H2. We propose that LC9-RNase H1 represents bacterial RNases H1 with an atypical DEDN active site motif, which are evolutionarily distinct from those with a typical DEDD active site motif.
Crystal Structure and Thermodynamic and Kinetic Stability of Metagenome-Derived LC-Cutinase
Sulaiman, Sintawee,You, Dong-Ju,Kanaya, Eiko,Koga, Yuichi,Kanaya, Shigenori American Chemical Society 2014 Biochemistry Vol.53 No.11
<P>The crystal structure of metagenome-derived LC-cutinase with polyethylene terephthalate (PET)-degrading activity was determined at 1.5 Å resolution. The structure strongly resembles that of <I>Thermobifida alba</I> cutinase. Ser165, Asp210, and His242 form the catalytic triad. Thermal denaturation and guanidine hydrochloride (GdnHCl)-induced unfolding of LC-cutinase were analyzed at pH 8.0 by circular dichroism spectroscopy. The midpoint of the transition of the thermal denaturation curve, <I>T</I><SUB>1/2</SUB>, and that of the GdnHCl-induced unfolding curve, <I>C</I><SUB>m</SUB>, at 30 °C were 86.2 °C and 4.02 M, respectively. The free energy change of unfolding in the absence of GdnHCl, Δ<I>G</I>(H<SUB>2</SUB>O), was 41.8 kJ mol<SUP>–1</SUP> at 30 °C. LC-cutinase unfolded very slowly in GdnHCl with an unfolding rate, <I>k</I><SUB>u</SUB>(H<SUB>2</SUB>O), of 3.28 × 10<SUP>–6</SUP> s<SUP>–1</SUP> at 50 °C. These results indicate that LC-cutinase is a kinetically robust protein. Nevertheless, the optimal temperature for the activity of LC-cutinase toward <I>p</I>-nitrophenyl butyrate (50 °C) was considerably lower than the <I>T</I><SUB>1/2</SUB> value. It increased by 10 °C in the presence of 1% polyethylene glycol (PEG) 1000. It also increased by at least 20 °C when PET was used as a substrate. These results suggest that the active site is protected from a heat-induced local conformational change by binding of PEG or PET. LC-cutinase contains one disulfide bond between Cys275 and Cys292. To examine whether this disulfide bond contributes to the thermodynamic and kinetic stability of LC-cutinase, C275/292A-cutinase without this disulfide bond was constructed. Thermal denaturation studies and equilibrium and kinetic studies of the GdnHCl-induced unfolding of C275/292A-cutinase indicate that this disulfide bond contributes not only to the thermodynamic stability but also to the kinetic stability of LC-cutinase.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/2014/bichaw.2014.53.issue-11/bi401561p/production/images/medium/bi-2013-01561p_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bi401561p'>ACS Electronic Supporting Info</A></P>
Hosogi, Hisahiro,Kanaya, Seiichiro,Nomura, Hajime,Kinjo, Yousuke,Tsubono, Michihiko,Kii, Eiji The Korean Gastric Cancer Association 2015 Journal of gastric cancer Vol.15 No.1
Laparoscopic distal gastrectomy has become widespread as a treatment for early gastric cancer in eastern Asia, but a standard method for setting the stomach transection line has not been established. Here we report a novel method of setting this line based on anatomical landmarks. At the start of the operation, two anatomical landmarks along the greater curvature of the stomach were marked with ink: the proximal landmark at the avascular area between the last branch of the short gastric artery and the first branch of the left gastroepiploic artery, and the distal landmark at the point of communication between the right and left gastroepiploic arteries. Just before specimen retrieval, the stomach was transected from the center of these two landmarks toward the lesser curvature. Then, about two-third of the stomach was reproducibly resected, and gastroduodenostomy was successfully performed in 26 consecutive cases. This novel method could be used as a standard technique for setting the transection line in laparoscopic distal gastrectomy.
Intracorporeal Anastomosis in Laparoscopic Gastric Cancer Surgery
Hosogi, Hisahiro,Kanaya, Seiichiro The Korean Gastric Cancer Association 2012 Journal of gastric cancer Vol.12 No.3
Laparoscopic gastrectomy has become widely used as a minimally invasive technique for the treatment of gastric cancer. When it was first introduced, most surgeons preferred a laparoscopic-assisted approach with a minilaparotomy rather than a totally laparoscopic procedure because of the technical challenges of achieving an intracorporeal anastomosis. Recently, with improved skills and instruments, several surgeons have reported the safety and feasibility of a totally laparoscopic gastrectomy with intracorporeal anastomosis. This review describes the recent technical advances in intracorporeal anastomoses using circular and linear staplers that allow for totally laparoscopic distal, total, and proximal gastrectomies. Data that demonstrate advantages in early surgical outcomes of a total laparoscopic method compared to laparoscopic-assisted operations are also discussed.