Ginsenoside Rd alleviates mouse acute renal ischemia/reperfusion injury by modulating macrophage phenotype

Kaixi Ren,Chao Jin,Pengfei Ma,Qinyou Ren,Zhansheng Jia,Daocheng Zhu 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.2

Background: Ginsenoside Rd (GSRd), a main component of the root of Panax ginseng, exhibits antiinflammation functions and decreases infarct size in many injuries and ischemia diseases such as focal cerebral ischemia. M1 Macrophages are regarded as one of the key inflammatory cells having functions for disease progression. Methods: To investigate the effect of GSRd on renal ischemia/reperfusion injury (IRI) and macrophage functional status, and their regulatory role on mouse polarized macrophages in vitro, GSRd (10e100 mg/ kg) and vehicle were applied to mice 30 min before renal IRI modeling. Renal functions were reflected by blood serum creatinine and blood urea nitrogen level and histopathological examination. M1 polarized macrophages infiltration was identified by flow cytometry analysis and immunofluorescence staining with CD11bþ, iNOSþ/interleukin-12/tumor necrosis factor-a labeling. For the in vitro study, GSRd (10 e100 mg/mL) and vehicle were added in the culture medium of M1 macrophages to assess their regulatory function on polarization phenotype. Results: In vivo data showed a protective role of GSRd at 50 mg/kg on Day 3. Serum level of serum creatinine and blood urea nitrogen significantly dropped compared with other groups. Reduced renal tissue damage and M1 macrophage infiltration showed on hematoxylineeosin staining and flow cytometry and immunofluorescence staining confirmed this improvement. With GSRd administration, in vitro cultured M1 macrophages secreted less inflammatory cytokines such as interleukin-12 and tumor necrosis factor-a. Furthermore, macrophage polarization-related pancake-like morphology gradually changed along with increasing concentration of GSRd in the medium. Conclusion: These findings demonstrate that GSRd possess a protective function against renal ischemia/ reperfusion injury via downregulating M1 macrophage polarization.

Ginsenoside Rd alleviates mouse acute renal ischemia/reperfusion injury by modulating macrophage phenotype

Ren, Kaixi,Jin, Chao,Ma, Pengfei,Ren, Qinyou,Jia, Zhansheng,Zhu, Daocheng The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.2

Background: Ginsenoside Rd (GSRd), a main component of the root of Panax ginseng, exhibits anti-inflammation functions and decreases infarct size in many injuries and ischemia diseases such as focal cerebral ischemia. M1 Macrophages are regarded as one of the key inflammatory cells having functions for disease progression. Methods: To investigate the effect of GSRd on renal ischemia/reperfusion injury (IRI) and macrophage functional status, and their regulatory role on mouse polarized macrophages in vitro, GSRd (10-100 mg/kg) and vehicle were applied to mice 30 min before renal IRI modeling. Renal functions were reflected by blood serum creatinine and blood urea nitrogen level and histopathological examination. M1 polarized macrophages infiltration was identified by flow cytometry analysis and immunofluorescence staining with $CD11b^+$, $iNOS^+$/interleukin-12/tumor necrosis factor-${\alpha}$ labeling. For the in vitro study, GSRd ($10-100{\mu}g/mL$) and vehicle were added in the culture medium of M1 macrophages to assess their regulatory function on polarization phenotype. Results: In vivo data showed a protective role of GSRd at 50 mg/kg on Day 3. Serum level of serum creatinine and blood urea nitrogen significantly dropped compared with other groups. Reduced renal tissue damage and M1 macrophage infiltration showed on hematoxylin-eosin staining and flow cytometry and immunofluorescence staining confirmed this improvement. With GSRd administration, in vitro cultured M1 macrophages secreted less inflammatory cytokines such as interleukin-12 and tumor necrosis factor-${\alpha}$. Furthermore, macrophage polarization-related pancake-like morphology gradually changed along with increasing concentration of GSRd in the medium. Conclusion: These findings demonstrate that GSRd possess a protective function against renal ischemia/reperfusion injury via downregulating M1 macrophage polarization.

miR-182-5p Inhibits NKAPL Expression and Promotes the Proliferation of Osteosarcoma

Shen Yang,Kaixi Chen,Kun Cao,Shenglin Xu,Chengxiao Ma,Yongping Cai,Yong Hu,Yejin Zhou 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.5

Purpose Osteosarcoma, a malignant bone tumor, has the lowest survival rate among all pediatric cancers. NF-κB-activating protein-like (NKAPL) is highly homologous with NKAP. The expression of NKAPL is downregulated in primary liver cancer and breast cancer, and plays a role of tumor suppressor gene. However, the role of NKAPL in osteosarcoma has not been reported. Materials and Methods We explored the effect of NKAPL on the proliferation of osteosarcoma cells by immunohistochemical, RT-PCR, Western blot, and double luciferase reporter gene analysis. Results The low expression of NKAPL mRNA was correlated with distant metastasis (P = 0.017), tumor size (P = 0.023), and clinical stage (P < 0.001). The NKAPL expression level in MG63 and U2OS cells was lower than that in Nhost cells. Downregulation of NKAPL expression in Nhost cells could promote cell proliferation and upregulation of NKAPL expression in MG63 and U2OS cells could inhibit cell proliferation. miR-182-5p expression was negatively correlated with NKAPL mRNA expression (R2 = 0.1169, P = 0.0099). After upregulating NKAPL expression, the Notch1, hes1, hey2, and cyclin D1 expression levels were significantly decreased, with G0/G1 phase arrest and G2/M phase reduction. Conclusions miR-182-5p targeted NKAPL and inhibit NKAPL expression in osteosarcoma. miR-182- 5p could regulate cell cycle and promote tumor proliferation through upregulating Notch signaling pathway.

Enhancement of resource recovery from retired tires: Extraction of copper and zinc resources

Xingyuan Wan,Kaixi Jiang,Ting’an Zhang,Han Yang,Kun Wang 대한환경공학회 2024 Environmental Engineering Research Vol.29 No.1

Dynamics of bubble formation and ascent motion on submerged orifices under different Mo number of petrol-based random copolymer solutions

Zhongyao Zhang,Xiaopeng Chen,Jiezhen Liang,Xiaojie Wei,Kaixi Deng,Jiezhong Zou,Linlin Wang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-

The petrol-based random copolymer, e.g., aromatic-hydrocarbon resin, is widely used in industrial production,but the research about its gas–liquid two flow is far from being solved. Herein, the formation,motion, and deformation of a single bubble in six different concentrations of random copolymer solutionswere systemically investigated using high-speed photography (1000 fps), combined with digital imageprocessing technology. The departure time of the bubble first decreases and then increases, and maximumof aspect ratio and specific surface area reduce as the proportion of solute increases. The bubbledeparture time decreases and bubble size increases with viscous force increases in the case of same surfacetension. Based on force balance analysis, added mass force is one of the prime reasons to affect bubbleoscillation during the acceleration phase after bubble detachment, whereas the effect of added massforce decreases as solute increases or as the bubble reaches stability zone. Also, velocity, drag coefficientand aspect ratio of bubble were discussed in detail by dimensionless analysis. Furthermore, three newshape numerical models (based on We, Ta, and WeEo, respectively) were proposed, which allowed for satisfactorypredictions, and new correlations represent well the experimental data in a wider range of Monumbers from the mentioned literature (11.6 logMo 0.63).

Component Prototyping for the China Spallation Neutron Source Project

Jie Wei,Yanwei Chen,Yunlong Chi,Changdong Deng,Haiyi Dong,Shinian Fu,Wei He,Kaixi Huang,Wen Kang,Jian Li,Huafu Ouyang,Huamin Qu,Caitu Shi,Hong Sun,Jingyu Tang,Juzhou Tao,Sheng Wang,Zhongxiong Xu,Xueju 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.5

The China Spallation Neutron Source (CSNS) complex consists of an H- linear accelerator, a rapid cycling synchrotron accelerating the beam to 1.6 GeV, a solid tungsten target station and instruments for spallation neutron applications. The facility operates at a 25-Hz repetition rate with an initial design beam power of 120 kW and is upgradeable to 500 kW. The primary challenge is to build a robust and reliable user-friendly facility with upgrade potential at a fraction of the \world standard" cost. Success of the project relies on the results of prototyping research & development (R&D) of key technical systems and components. This paper discusses the prototyping experiences of the past two and a half years. The China Spallation Neutron Source (CSNS) complex consists of an H- linear accelerator, a rapid cycling synchrotron accelerating the beam to 1.6 GeV, a solid tungsten target station and instruments for spallation neutron applications. The facility operates at a 25-Hz repetition rate with an initial design beam power of 120 kW and is upgradeable to 500 kW. The primary challenge is to build a robust and reliable user-friendly facility with upgrade potential at a fraction of the \world standard" cost. Success of the project relies on the results of prototyping research & development (R&D) of key technical systems and components. This paper discusses the prototyping experiences of the past two and a half years.