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Kouki Sasaka,Qingyue Wang,Kazuhiko Sakamoto 한국대기환경학회 2017 Asian Journal of Atmospheric Environment (AJAE) Vol.11 No.3
In Japan, the primary carbonaceous particles emitted from motor vehicles and waste incinerators have been reduced due to strict regulations against exhaust gas. However, the relative contribution of carbonaceous particles derived from plants and biomass has been increasing. Accordingly, compositional analysis of carbonaceous particles has become increasingly important to determine the sources and types of particles produced. To reveal the sources of the organic particles contained in particulate matter with diameters of ≤ 2.5 μm (PM2.5) and the processes involved in their generation, we analyzed molecular marker compounds (2-methyltetrols, cis-pinonic acid, and levoglucosan) derived from the plants and biomass in the PM2.5 collected during daytime- and nighttime-sampling periods in summer (July and August) and autumn (November) in Kazo, which is in the northern area of Saitama prefecture, Japan. We also measured 14C carbonaceous concentrations in the same PM2.5 samples. The concentrations of 2-methyltetrols were higher in the summer than in the autumn. Because the deciduous period overlaps with this decrease in the levels of 2-methyltetrols, we considered the emission source to broad-leaved trees. In contrast, the emission source of the cispinonic acid precursor was considered to be conifers, because its concentration remained almost constant throughout the year. The concentration of levoglucosan was considerably increased in the autumn due to frequent biomass open burning. The ratio of plantderived carbon to total carbon, obtained by measuring of 14C, in summer PM2.5 sample was higher in the nighttime, and could be influenced by anthropogenic sources during the daytime.
Kouki Iwata,Katsuyuki Eguchi,Seiki Yamane 한국응용곤충학회 2005 Journal of Asia-Pacific Entomology Vol.8 No.3
Ant fauna of a green buffer belt adjacent toan industrial zone in Kagoshima City, southern Kyushu, Japan was elucidated using a standardized protocol. A total of 39 species belonging to 24 genera in five subfamilies were recorded from this park. Mostspeciose genera are Camponotus and Tetramoriumhaving four species. The number of species per genus is 1.56 on average. Pheidole noda F. Smith, Monomo-rium chinense Santschi, Solenopsis japonica Wheeler, Tetramorium bicarinatum (Nylander) and Technomyrmexalbipes (F. Smith) were most frequently encountered. Excepting Pheidole noda they are open-land and/or forest-edge inhabitants. Furthermore, Tetramorium bica-rinatum and Technomyrmex albipes are tramp species. Thus, ant fauna there strongly reflects environmental conditions derived from urbanization/industrialization. Based on the present results, we propose here a new protocol monitoring ants in urban/industrial zonesconsisting of baiting and time unit sampling.
Kouki Tsuji,Huimin Lu,Joo Kooi Tan,Hyoungseop Kim,Kazue Yoneda,Fumihiro Tanaka 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10
Circulating tumor cells (CTCs) is a useful biomarker for cancer metastasis. The blood from a cancer patient is analyzed by a fluorescence microscope. Each case takes a large number of images, which usually have a lot of cell regions. Thus, analyzing the images is hard work for pathologists, and misdiagnosis may happen. In this paper, we develop an automatic CTCs identification method for fluorescence microscopy images. The proposed method consists of three steps. First, we extract cell regions in images using filtering methods. Second, we compute features of each CTC candidate regions. Finally, we identify the CTCs using AdaBoost algorithm. And we analyze the features to know which ones are effective for characterizing CTCs and normal cells. We apply the proposed method to 5040 microscopy images, and evaluate the effectiveness of our method by using leave-one-out cross validation. We achieve a true positive rate of 97.30 [%] and a false positive rate of 12.82 [%].
Kouki Tsuji,Joo Kooi Tan,Hyoungseop Kim,Kazue Yoneda,Fumihiro Tanaka 제어로봇시스템학회 2016 제어로봇시스템학회 국제학술대회 논문집 Vol.2016 No.10
Circulating tumor cells (CTCs) is an informative biomarker which assists pathologists in early diagnosis and evaluating therapeutic effects of patients with malignant tumors. The blood from a cancer patient is analyzed by a microscope and a large number of pictures including many cells are generated for each case. Thus, analyzing them is time-consuming work for pathologists, and misdiagnosis may happen since the diagnosis of CTCs tends to depend on the individual skill of pathologist. In this paper, we propose a method which detects cell candidate regions in microscopy images automatically to make quantitative analysis possible by computer. Our proposed method consists of three steps. In the first step, we extract initial cell candidate regions in microscopy images based on the saliency map. In the second step, we choose non-single cell regions from the initial candidates based on the SVM algorithm. In the third step, we separate connected regions into single cell regions based on the branch and bound algorithm. We demonstrated the effectiveness of our proposed method using 540 microscopy images and we achieved a true positive rate of 99.04[%] and a false positive rate of 3.95[%].
Imaoka Kouki,Yano Takuya,Yoshimitsu Masanori,Fukuhara Sotaro,Oshita Ko,Nakano Kanyu,Kunihiro Masaki,Idani Hitoshi,Okajima Masazumi 대한대장항문학회 2023 Annals of Coloproctolgy Vol.39 No.2
Purpose: Harvesting at least 12 lymph nodes (LNs) is recommended for adequate tumor staging in colon surgery. Although preoperative endoscopic tattooing has been used for primary localization of tumors, its impact on LN retrieval in colorectal surgery remains controversial. We aimed to investigate the relationship between preoperative tattooing and LN retrieval after laparoscopic rectal resection.Methods: We reviewed the records of 92 patients with rectal cancer who underwent laparoscopic resection from January 1, 2018 to December 31, 2019. Patients were categorized into 2 groups according to whether preoperative endoscopic tattooing was performed. The rate of adequate LN retrieval (≥12) was compared.Results: The tattooed and non-tattooed groups comprised 49 and 43 patients, respectively. In the tattooed and non-tattooed groups, the rates of adequate LN retrieval were 75.5% and 55.8%, respectively (P=0.046). Univariate analysis revealed that female sex, tattooing, LN metastasis status, pathological pathological stage (p-stage), and LN dissection were predictive factors for adequate LN retrieval. In the multivariate analysis, female sex (odds ratio [OR], 3.34; 95% confidence interval [CI], 1.15–9.73; P=0.027), tattooing (OR, 2.87; 95% CI, 1.03–7.94; P=0.043), and p-stage (OR, 3.34; 95% CI, 1.04–10.75; P=0.043) were independent predictive factors for adequate LN retrieval after surgery.Conclusion: This study revealed that preoperative endoscopic tattooing was statistically significantly associated with adequate LN retrieval in patients with rectal cancer who underwent laparoscopic rectal resection. Preoperative endoscopic tattooing should be considered to improve disease assessment and avoid stage migration.