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( Juree Kim ),( Jungwon Choi ),( Sam Sik Kang ),( Sanghyun Lee ) 한국응용생명화학회(구 한국농화학회) 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.1
Qantitative analysis of six compounds: (+)-catechin, benzoic acid, gallic acid methyl ester, paeonol, paeoniflorin, and albiflorin from Paeonia lactiflora extracts was performed using high-performance liquid chromatography and an ultraviolet (UV) detector, following different extraction methods. A reverse-phase column was used in a gradient elution system, and UV detection was performed at 280 nm. The results showed that the quantity of paeoniflorin was the highest in ethanol and water extracts (73.89 and 57.87 mg/g, respectively) among the six compounds. This study contributes a good analysis method for the contents of P. lactiflora and would be propitious for developing medicines and functional foods.
Phytochemical constituents of Lactuca serriola leaves and their content analysis by HPLC-UV
Juree Kim,Hak-Dong Lee,Jungwon Choi,Sanghyun Lee 한국응용생명화학회 2022 Journal of Applied Biological Chemistry (J. Appl. Vol.65 No.3
This study aimed to identify the phytochemical constituents of Lactuca serriola leaves and perform quantitative analysis of the methanol (MeOH) extract of L. serriola, L. indica, L. raddeana, L. sativa, and L. triangulata. Six compounds were isolated from the MeOH extracts of L. serriola using open column chromatography and identified as protocatechuic acid (1), caffeic acid (2), cynaroside (3), apigenin 7-glucuronide (4), luteolin (5), and apigenin (6) using 1H-, 13C-nuclear magnetic resonance, and mass spectrometry. Quantitative analysis of the six compounds was performed on the MeOH extract of Lactuca species using high-performance liquid chromatography (HPLC) and an ultraviolet (UV). A reverse-phased column was used, and the UV absorbance was set to 280 nm. The contents of compounds 2 and 3 were the highest (1.58 and 2.64 mg/g ext., respectively) in L. serriola extracts. However, compounds 4 and 6 were higher (1.46 and 0.40 mg/g ext., respectively) in L. triangulata. These results provide quantitative data for the application of Lactuca species in the pharmaceutical and functional food industries.
( Juree Kim ),( Taewon Jang ),( Ji Hyun Kim ),( Hanna Shin ),( Jaeho Park ),( Sanghyun Lee ) 한국응용생명화학회 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.3
The in vitro anti-obesity activity of 12 species of tree sprouts in differentiated 3T3-L1 cells and the mechanisms underlying their activity were evaluated. (+)-Catechin and quercetin concentrations in the sprouts were analyzed by HPLC/ UV at 270 and 254 nm, respectively. Euonymus alatus (EAT) and Fraxinus mandschuria (FMS) extracts at doses of 50 and 100 μg/ mL inhibited the accumulation of lipid droplets in differentiated 3T3-L1 cells. Moreover, EAT and FMS downregulated the expression of the CCAAT/enhancer-binding protein-α, adipogenesisrelated proteins peroxisome proliferator-activated receptor-γ, and adipocyte P-2α in differentiated 3T3-L1 cells. Tree sprouts with an abundant flavonoid content exerted the highest anti-obesity activity. Concentrations of total flavonoids were the highest in FMS (24.281 mg/g DW) sprouts. These findings could be used to develop health-promoting functional foods or supplements derived from tree sprouts.
Graphene as an atomically thin barrier to Cu diffusion into Si
Hong, Juree,Lee, Sanggeun,Lee, Seulah,Han, Heetak,Mahata, Chandreswar,Yeon, Han-Wool,Koo, Bonwoong,Kim, Seong-Il,Nam, Taewook,Byun, Kisik,Min, Byung-Wook,Kim, Young-Woon,Kim, Hyungjun,Joo, Young-Chang The Royal Society of Chemistry 2014 Nanoscale Vol.6 No.13
<P>The evolution of copper-based interconnects requires the realization of an ultrathin diffusion barrier layer between the Cu interconnect and insulating layers. The present work reports the use of atomically thin layer graphene as a diffusion barrier to Cu metallization. The diffusion barrier performance is investigated by varying the grain size and thickness of the graphene layer; single-layer graphene of average grain size 2 ± 1 μm (denoted small-grain SLG), single-layer graphene of average grain size 10 ± 2 μm (denoted large-grain SLG), and multi-layer graphene (MLG) of thickness 5-10 nm. The thermal stability of these barriers is investigated after annealing Cu/small-grain SLG/Si, Cu/large-grain SLG/Si, and Cu/MLG/Si stacks at different temperatures ranging from 500 to 900 °C. X-ray diffraction, transmission electron microscopy, and time-of-flight secondary ion mass spectroscopy analyses confirm that the small-grain SLG barrier is stable after annealing up to 700 °C and that the large-grain SLG and MLG barriers are stable after annealing at 900 °C for 30 min under a mixed Ar and H2gas atmosphere. The time-dependent dielectric breakdown (TDDB) test is used to evaluate graphene as a Cu diffusion barrier under real device operating conditions, revealing that both large-grain SLG and MLG have excellent barrier performance, while small-grain SLG fails quickly. Notably, the large-grain SLG acts as a better diffusion barrier than the thicker MLG in the TDDB test, indicating that the grain boundary density of a graphene diffusion barrier is more important than its thickness. The near-zero-thickness SLG serves as a promising Cu diffusion barrier for advanced metallization.</P>
Capillary Assembly of Silicon Nanowires Using the Removable Topographical Patterns
Hong, Juree,Lee, Seulah,Lee, Sanggeun,Seo, Jungmok,Lee, Taeyoon Materials Research Society of Korea 2014 한국재료학회지 Vol.24 No.10
We demonstrate a simple and effective method to accurately position silicon nanowires (Si NWs) at desirable locations using drop-casting of Si NW inks; this process is suitable for applications in nanoelectronics or nanophotonics. Si NWs were assembled into a lithographically patterned sacrificial photoresist (PR) template by means of capillary interactions at the solution interface. In this process, we varied the type of solvent of the SiNW-containing solution to investigate different assembly behaviors of Si NWs in different solvents. It was found that the assembly of Si NWs was strongly dependent on the surface energy of the solvents, which leads to different evaporation modes of the Si NW solution. After Si NW assembly, the PR template was cleanly removed by thermal decomposition or chemical dissolution and the Si NWs were transferred onto the underlying substrate, preserving its position without any damage. This method enables the precise control necessary to produce highly integrated NW assemblies on all length scales since assembly template is easily fabricated with top-down lithography and removed in a simple process after bottom-up drop-casting of NWs.
Antioxidant activity of edible sprouts and phytosterol contents by HPLC/UV analysis
Kim Juree,Kim Ji Hyun,방세인,Shin Hanna,Cho Eun Ju,Lee Sanghyun 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.5
This study evaluated the in vitro radical scavenging activities of edible tree sprouts, particularly those of the 2,2-diphenyl- 1-picrylhydrazyl radical (DPPH), hydroxyl radical, and singlet oxide radical, to assess their antioxidant activities. Addi- tionally, stigmasterol (ST) and β-sitosterol (BS) were analyzed using HPLC/UV. The edible sprouts of Eleutherococcus senticosus (ESC) and Morus alba (MAB) exhibited the highest DPPH scavenging activity among other edible sprouts. A reverse-phase column was used in an isocratic elution system, after which UV detection was performed at 210 nm. ST and BS analyses indicated that ESC sprouts contained the highest amounts of ST (9.99 mg·g− 1 extract), whereas MAB sprouts contained the highest concentrations of BS (14.69 mg·g− 1 extract). In conclusion, the highest antioxidant activity was observed in the edible sprouts with the highest phytosterol content. Therefore, our findings provide a theoretical basis for the development of plant-based functional foods or supplements with antioxidant properties.
최주리(Choi Juree),이민수(Lee Minsoo) 한국정보과학회 2005 한국정보과학회 학술발표논문집 Vol.32 No.1
센서 네트워크에서 집계(aggregation) 연산은 센서 네트워크를 데이터베이스로 구현하는데 있어서 중요하게 제공되어져야 하는 서비스이다. 현재 연구되고 있는 것으로 집계 연산을 센서 네트워크의 특징상으로 분류하여 근접한 결과값을 받는 것을 허용하고 집계 값을 자식노드가 부모노드로 보내는 기간을 부모 노드가 자식노드에게 나누어 할당하여 센서 네트워크상에 적절히 구현하는 것에 대해 알아보고 집계 그루핑을 하는 과정에서 데이터웨어하우징 연구의 최신기술인 점진적인 뷰 관리 기법을 통해 센서노드들의 평균값에 대해 새로운 값이 추가될 때 다시 모든 값을 계산하기 않고 변경된 값만 적용하여 계산함으로 좀 더 에너지 효율적으로 확장하는 것을 제안하였다.