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( Jun Tao ),( Fa-liang An ),( Zheng-hua Pan ),( Yan-hua Lu ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.5
Biofilms are of vital significance in bioconversion and biotechnological processes. In this work, sugarcane molasses was used to enhance biofilms for the improvement of the production of fumigaclavine C (FC), a conidiation-associated ergot alkaloid with strong anti-inflammatory activities. Biofilm formation was more greatly induced by the addition of molasses than the addition of other reported biofilm inducers. With the optimal molasses concentration (400 g/l), the biofilm biomass was 6-fold higher than that with sucrose, and FC and conidia production was increased by 5.8- and 3.1-fold, respectively. Moreover, the global secondary metabolism regulatory gene laeA, FC biosynthetic gene fgaOx3, and asexual central regulatory genes brlA and wetA were upregulated in molasses-based biofilms, suggesting the upregulation of both asexual development and FC biosynthesis. This study provides novel insight into the stimulatory effects of molasses on biofilm formation and supports the widespread application of molasses as an inexpensive raw material and effective inducer for biofilm production.
Jun Tao,Zhaojun Hao,Mengran Wei,Saijie Gong,Daqiu Zhao 한국유전학회 2016 Genes & Genomics Vol.38 No.12
Herbaceous peony (Paeonia lactiflora Pall.) is easily injured by heat stress (HS), which greatly restricts its application and promotion. In this study, the thermo-tolerance of three representative P. lactiflora cultivars had been firstly assessed. ‘Zifengyu’ was identified as the thermo-tolerant cultivar with relatively lower values and smaller variations in malondialdehyde, hydrogen peroxide (H2O2) and proline contents under HS. Subsequently, their transcriptomes were sequenced by RNA sequencing (RNAseq) technology to construct a complete database. 81,599 unigenes were obtained, and 34,940 unigenes had been annotated. Moreover, through digital gene expression analysis of thermo-tolerant ‘Zifengyu’ and moderately thermo-tolerant ‘Hongyanzhenghui’, 161 heat stress response genes had been screened involving heat shock protein genes, plant hormone signal transduction related genes, fatty acid synthesis genes, reactive oxygen speciesscavenging genes and secondary metabolites related genes. And the effectively and timely response of these genes to HS could endow thermo-tolerance to ‘Zifengyu’. Among these genes, 11 key thermo-tolerant related genes whose expressions were all significantly up-regulated in ‘Zifengyu’ and ‘Hongyanzhenghui’ during development and the former possessed higher levels could be regarded as the candidate genes, including isoprene synthase gene, 2 peroxidase genes, 3-oxoacyl-acyl carrier protein reductase gene (FabG), 3 transcription factor genes (bHLH, NAC and WRKY), HSP20 and 3 HSP70. These results could provide a better understanding of heat stress response in P. lactiflora, and pave for the breeding of thermo-tolerant cultivars.
Adaptive Multi-class Support Vector Machine for Microarray Classification and Gene Selection
Juntao Li,Yingmin Jia,Junping Du,Fashan Yu 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
This paper proposes an adaptive multi-classs upport vector machine for simultaneous microarray classification and gene selection. By evaluating the gene ranking significance, the adaptive multi-class support vector machine is shown to encourage an adaptive grouping effect in the process of building classifiers, thus leading as parse multi-classifiers with enhanced interpretability. Based on a reasonablec orrelation between the two regularization parameters, an efficient solution path algorithm is developed for solving the proposed support vector machine. Experiments performed on the leukaemia data set are provided to verify the obtained results.
Juntao Yao,Rong Jia,Leilei Zheng,Bangxing Wang 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.5
The production of ligninolytic enzymes by the fungus Schizophyllum sp. F17 using a cost-effective medium comprised of agro-industrial residues in solid-state fermentation (SSF) was optimized. The maximum activities of the enzymes manganese peroxidase (MnP), laccase (Lac), and lignin peroxidases (LiP) were 1,200, 586, and 109 U/L,respectively, on day 5 of SSF. In vitro decolorization of three structurally different azo dyes by the extracellular enzymes was monitored to determine its decolorization capability. The results indicated that crude MnP, but not LiP and Lac, played a crucial role in the decolorization of azo dyes. After optimization of the dye decolorization system with crude MnP, the decolorization rates of Orange IV and Orange G, at an initial dye concentration of 50 mg/L, were enhanced to 76 and 57%, respectively, after 20 min of reaction at pH 4 and 35oC. However, only 8% decolorization of Congo red was observed. This enzymatic reaction system revealed a rapid decolorization of azo dyes with a low MnP activity of 24 U/L. Thus, this study could be the basis for the production and application of MnP on a larger scale using a low-cost substrate.
Juntao Yang,Seungman Baek 대한건축학회 2021 Architectural research Vol.23 No.2
Early in the communist era, many cities in modern China experienced a special period of passive industrialization during which the construction of urban spaces laid the spatial urban foundation. During this period, the city construction was carried out rapidly under the administrative intervention of the government. The passive industrialization caused by taking economic construction as the center made the city construction deviate from the correct track and resulted in a variety of problems. Focusing on ecological space change, this paper selects Zhengzhou as an example of many similar cities in China, and analyzes the impact of passive industrialization on urban ecological spaces from the perspectives of city scale, city layout and city construction management system. The analysis results reveal that the construction of urban ecological space under the influence of passive industrialization induced a lagging characteristic, led to insufficient integrity of urban ecological space, and placed great pressure on the urban environment. Finally, the paper puts forward some methods to optimize the urban ecological space.
Adaptive Backstepping Sliding Mode Control for MEMS Gyroscope
Juntao Fei,MingyuanXin,Weili Dai 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10
In this paper, an adaptive backstepping sliding mode control approach is proposed to attenuate the tracking error and control the MEMS triaxial gyroscope. An adaptive backstepping controller that can compensate the system nonlinearities is incorporated into the sliding mode control scheme in the Lyapunov framework. First, a robust backstepping control system incorporated with sliding mode control technique is designed. Then, an adaptive law is derived to estimate the upper bound value of the lumped uncertainty in the backstepping sliding mode control. With the adaptive backstepping sliding mode controller, the chattering in control efforts can be obviously reduced. Numerical simu lation is provided to verify the effectiveness of the proposed scheme.
Positive solutions of singular Dirichlet problems via variational methods
Juntao Sun,Jifeng Chu 대한수학회 2013 대한수학회지 Vol.50 No.4
In this paper, we establish the existence results for secondorder singular Dirichlet problems via variational methods. Some recentresults are extended and improved. Examples are also given to illustratethe new results.
Analyze Requirements for PAIS with Best Practice
Juntao GAO,Xueshan Wang,Yongan WANG 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.2
Traditional requirements analysis is focused on capture functions of the system that are being built from scratch. As the size and complexity of process-aware information system continues to grow, the use of COTS (Commercial off-the-shelf) software packages is being viewed as a solution. Use of COTS packages accelerates the implementation process to some extent, however, it puts enterprises into a dilemma: whether to adapt as-is business processes or to customize software packages. In order to help the enterprises get out of the dilemma, a methodology is proposed to automatically elicit software requirement using best practice as domain knowledge. The methodology involves three phases: business modeling, gap detecting and gap bridging. Firstly, VPML is employed to describe as-is business process, Secondly, semantics computing technology is employed to analyze the gap between enterprise needs and COTS software capacity. At last, Goal Reasoning Technology is employed to encourage enterprise users and software vendors take participate in decision making process together.
Consensus Seeking of Multi-agent Systems from an Iterative Learning Perspective
Juntao Li,Yadi Wang,Huimin Xiao 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.5
The consensus seeking problems for both discrete and continuous multi-agent networks are discussedfrom an iterative learning perspective. It is shown that the consensus seeking process can be viewed as an iterativelearning process for agents under directed networks to improve their performances from time to time in order toachieve consensus. If a desired consensus state is specified, then the multi-agent system can be guaranteed toreach consensus through reducing the tracking error between each agent’s state and the desired consensus statemonotonically to zero with respect to the increasing of time. If there is no desired consensus state, then the agentscan achieve consensus through reducing their states monotonically to the minimum quantity with increasing time. Simulations illustrate the observed results.