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Jungsu Park,Yongje Kim,Minjae Kim,Woo Hyoung Lee 대한환경공학회 2019 Environmental Engineering Research Vol.24 No.3
Microcystis sp. is one of the most common harmful cyanobacteria that release toxic substances. Counting algal cells is often used for effective control of harmful algal blooms. However, Microcystis sp. is commonly observed as a colony, so counting individual cells is challenging, as it requires significant time and labor. It is urgent to develop an accurate, simple, and rapid method for counting algal cells for regulatory purposes, estimating the status of blooms, and practicing proper management of water resources. The flow cytometer and microscope (FlowCAM), which is a dynamic imaging particle analyzer, can provide a promising alternative for rapid and simple cell counting. However, there is no accurate method for counting individual cells within a Microcystis colony. Furthermore, cell counting based on two-dimensional images may yield inaccurate results and underestimate the number of algal cells in a colony. In this study, a three-dimensional cell counting approach using a novel model algorithm was developed for counting individual cells in a Microcystis colony using a FlowCAM. The developed model algorithm showed satisfactory performance for Microcystis sp. cell counting in water samples collected from two rivers, and can be used for algal management in fresh water systems.
The Purchase Behavior of HMR for SAFETY Food Culture for the Elderly People
Jungsu Yang,김현아,Bonghyuk Choi J-INSTITUTE 2020 International Journal of Crisis & Safety Vol.5 No.2
Purpose; Currently, the silver industry is developing very much, and research on elderly aging food is needed for the elderly who are having a hard time digesting and chewing food. In order to develop HMR for the elderly, the results were derived through FGI(Focus Group Interview) to identify the purchase status of HMR for the elderly, to identify satisfaction and importance through IPA analysis, and to present the needs and development directions for the elderly food development. Method: A total of six consumers aged 60 or older were interviewed(December 28-30, 2019), and the content of the interview was that for elderly consumers with experience in purchasing HMR each interview took two to a half hours to record a maximum of two and a half hours. Results: There were six women in gender, and four under 65 age to 70 age. HMR was frequently pur-chased(once or three times a week) and purchased by four people, due to the convenience of purchase(simple), four large discount stores were purchased by HMR. Preferred food ingredients were found to prefer beef, spinach, cucumber, brown rice, fermented milk, apples and strawberries. The IPA results showed that the importance and satisfaction were high in taste, nutritional value, various menus, and convenience of food acquisition. Conclusion: It is necessary to develop various menus that suit the tastes of the elderly so that they can safely protect the elderly from health and lead their lives. In the next study, it is thought that the size of food that the elderly can chew with their teeth should also be studied to help them eat more comfortably. It is also thought that a detailed study on the level of chewing and swallowing food should be conducted depending on the condition of the teeth of the elderly.
Thermo-sensitive Electrospun Fibrous Magnetic Composite Sheets
Jungsu Choi,Jinu Kim,Heejae Yang,Frank K. Ko,Ki Hyeon Kim 한국자기학회 2015 Journal of Magnetics Vol.20 No.3
The PVDF fibrous composite filled with iron oxide nanoparticles were prepared by using the electrospinning technique. The electrospun composite have the thickness in the range of 60-80 μm with the average fibrous diameters of 500-900 nm. The magnetizations of PVDF fibrous composite filled with iron oxide nanoparticles showed 4.5 emu/g, 3.1 emu/g and 1.6 emu/g at 1.5 T of external magnetic field for 20 wt.%, 10 wt.% and 5 wt.% iron oxide nanoparticles, respectively. The heat elevation of the magnetic composite were measured under various AC magnetic fields, frequency and the ambient temperatures. The temperature reached up to 46.3℃ from 36oC at 128 Oe and 355 kHz for 20 wt.% iron oxide nanoparticles filled in PVDF fibrous composite sheet. The specific absorption rate of theses sheets increased from 0.041 W/g to 0.236 W/g with the increment of AC magnetic field from 90 Oe to 167 Oe at 190 kHz, respectively.
Development of PCR-based molecular markers by transcriptome sequencing in Platycodon grandiflorume
Jungsu Jung,Hyun Jung Kim,Je Min Lee,Myung-Shin Kim,Doil Choi,Inhwa Yeam 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Platycodon grandiflorum, which is the only species in the genus Platycodon of the family Campanulaceae, is an herbaceous flowering perennial. P. grandiflorum is generally known as bellflower or balloon flower indicating its ornamental uses. It has also been traditionally used as a medicinal crop in East Asia, which is widely employed as an antiphlogistic, antitussive, and expectorant. However, marker-assisted selection and molecular breeding in P. grandiflorum has lagged behind other plants such as pepper and tomato because of the lack of genetic information and effective molecular markers. Transcriptome sequencing provides an effective way to obtain large amount of sequence data when there is no available genome sequence. In this study, we performed a transcriptome analysis in platycodons, which has not been attempted previously. We analyzed simple sequence repeats (SSRs) using RNA-seq data. Di-nucleotide motifs were the most abundant repeats (39% ~ 40%) followed by mono- (26% ~ 32%), tri- (25% ~ 31%), tetra- (1.5% ~ 1.9 %), penta- (0.3% ~ 1%) in three platycodon accessions. Based on the SNP information obtained from RNA-seq analysis, we developed 12 PCR-based markers in Platycodon. The number of alleles ranged from two to seven with the average PIC value of 0.373. These 12 markers were applied to 21 platycodon accessions and a phylogenetic tree was constructed. The markers developed in this study could be introduced in molecular breeding program of platycodons. The SSR information obtained from RNA-seq analysis could be further utilized for developing genic-SSR markers in platycodons. Since platycodon is considered as an orphan crop, which has not been actively deployed for genetic study, the sequence information obtained from this study will contribute to further genetic improvements, genomic information and gene discovery in platycodon