Earnings Management by Controlling Shareholders in Anticipation of Stock Gift Transactions

Woon?Oh Jung,Sung?Ook Park 한국경영교육학회 2009 한국경영교육학회 학술발표대회논문집 Vol.2009 No.12

Linear Combination Analysis Using GPS Data

Park, Woon-Yong,Lee, Dong-Rak,Back, Ki-Suk,Hong, Jung-Soo 東亞大學校 建設技術硏究所 2005 硏究論文集 Vol.28 No.2

We can process and compute the position, velocity and time by satellite signals of GPS. The signals are used to compute positioning of three dimensions and timing offset of the receiver clock when we can track the tour satellite signals at least. One of the specified aims is to use less expensive single frequency code/carrier phase GPS receivers, which are typically around half the price of dual frequency receivers. In the study, the author analyzed the accuracy and applicability of frequence linear combination using triangulation points evaluated distance limitation.

세균성 질환진단 검사기술 개발동향과 임상검사에의 적용

정운원 청주대학교 보건의료과학연구소 2019 보건의료과학연구 Vol.7 No.2

Conventional culture-based identification systems are well standardized and widely adopted to detect and identify infectious pathogens in the microbiological diagnostics. These traditional methods as gold standard have the advantages of high sensitivity, high specificity and low cost, whereas they shows the disadvantages on intensive labor, long test time and non-growing some microorganisms that not grow in artificial medium. A fast and reliable identification of pathogens is a keyfacts in clinical diagnostics. In order to meet these problems, the new technological methods has been applied on the identification of clinically important pathogens over the last decades. Although microbiological analysis is too complexed to automate because of the various reasons, automation of culture system is becoming a reality in recent years in microbiological laboratories. Revolutionary nucleic acid analysis such as polymerase chain reaction(PCR), hybridization, sequencing and others has been increasingly used and considered to compensate or replace traditional culture-based methods in the future. Another technique is the matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF/MS). These new methods do not need bacterial culture to confirm the pathogens and have fast, easy and high-throughput features. Along with the more advanced technologies to be able to identify pathogens accurately, the clinical applications of them in microbial laboratories will be sustainedly performed. The recent trend of microbial diagnostic technologies and their clinical applications in microbiological laboratories would be discussed in this review.

임상 분자진단에서의 Digital PCR의 유용성

정운원 청주대학교 보건의료과학연구소 2015 보건의료과학연구 Vol.4 No.1

In molecular diagnostics, the methodological development and application of detecting and quantifying nucleic acid has been made over the past decades. Nucleic acid analysis based polymerase chain reaction(PCR) has been dominated among various techniques, such as PCR, sequencing, microarray and others. Detection and quantification of disease-related genes gives us a important information that may be used to predict disease progression, signs of infection and the efficacy of therapeutic agent. traditional PCR is based on exponential amplification and quantification of nucleic acids may be determined by comparing the number of amplification cycles and amount of PCR product to those of a reference specimen. However, many factors can complicate the calculation of quantification, resulting in uncertainties and inaccuracies. Digital PCR is considered to have overcome the critical problems of insufficient quantification in traditional PCR. The difference between dPCR and traditional PCR lies on the measuring method for nucleic acids amounts. Absolute quantification through clonal amplification is a key factor in digital PCR. It has many possible applications which include the detection and quantification of low-level pathogens, viral load, rare allele, copy number variation, and relative gene expression. Along with the more advanced development in technology of digital PCR, the clinical applications of digital PCR will be greatly expanded. Therefore, the present review describes the principle, technological types and clinical applications of digital PCR as useful molecular diagnostic tool.

정상과 만성염증성 활막배양세포에서 Corticotropin releasing hormone(CRH)이 cyclooxygenaase-1(COX-1)과 cyclooxygenase-2(COX-2) mRNA발현에 미치는 영향

정운원,이승관,이창규,조경진,김성욱,하주희,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2000 保健科學論集 Vol.26 No.1

Corticotropin-releasing hormone(CRH) is a major regulator of the hypothalamic-pituitaryadrenal axis(HPA). In inflammatory stress, the cytokines TNF-, IL-6 stimulate the production of CRH, a 41 amino acids neuropeptide, in the hypothalamus. The release of CRH leads to pituitary production of adrenocorticotropic hormone, followed by glucocorticoid secretion by the adrenal cortex. Glucocorticoids suppress namy components of the inflammatory process. Recently, CRH and CRH receptor were reported to be located in the periphery such as Immune system and chronic inflammatory sites as rheumatoid arthritis (RA). Cyclooxygenase consisted of two isoforms, COX-1 and COX-2, converts arachdonate to prostaglandins(PGs) which are important mediators of inflammation. insymoviocyte in RA, it was described that COX-2 mRNA was markedly increased by inflammatory agents, PMA or IL-1 and COX-1 transcripts were not modulated. We examined the modulation of COX by immune CRH in cultured normal and rheumatoid synoviocytes. Our results were shown that COX-1 mRNA expression decreased with the each stimulation of PMA and IL-1 in normal synoviocyte. In RA synoviocyte, PMA and IL-1 were increased mRNA expression of COX-1. In simultaneous treatment with CRH, PMA group was decreased, but IL-1 group was increased mRNA expression of COX-1. COX-2 mRNA expression was slightly increased by the treatment with PMA and highly increased by IL-1. After CRH treatment, PMA and IL-1 addictively increased COX-2 mRNA expression. We think that these results are contributed to the influence of increased cANP by CRH on the promoter of COX-2 in normal and synoviocytes. Because many cytokines, neuropeptides, and signal transduction pathways are involved in chronic inflammation, the exact role of CRH on inflammation is not fully elucidated. To achieve this goal, further experiments are needed.

임상 분자진단검사의 진단기법 동향

정운원 청주대학교 보건의료과학연구소 2014 보건의료과학연구 Vol.3 No.1

Over the past decades, the development and application of molecular diagnostics has been made in all fields of laboratory medicine as in vitro diagnostics. Although conventional methods are mainly used, there is an increasing trend towards molecular diagnostics. These techniques are superior to conventional ones in rapid detection, higher sensitivity and specificity. Polymerase chain reaction(PCR)-based systems has dominated in various nucleic acid-based techniques, such as PCR, sequencing, DNA microarray, lab-on-a-chip(LOC) and etc. PCR-based systems can detect the etiologic agents through the amplification of disease-related gene directly from clinical samples and have been undergone various modification for more rapid, accurate and applicable to diagnostic tests. Sequencing analysis ensures exact identification and better characterization of the etiologic agents. Some like array-based systems offer the multiparameter results where many pathogen-related markers and gene mutations can be analysed simultaneously. The development of LOC chip should allow point-of-care testing for the revolutionary improvement of healthcare. Along with the development of proteomic or metabolomic-based tests, More advanced diagnostic tools will expand the applications of molecular diagnostic testing. The user-friendly automation about both the high throughput and the use of minimal quantity of sample makes these technologies more widely available.

일측전정기과 손상 희쥐의 전정보상과정에서 뇌간 신경핵의 cAMP-Response Element Binding 단백질 활성화

윤상대,이정헌,김진호,김민선,박병림 대한이비인후과학회 2001 대한이비인후과학회지 두경부외과학 Vol.44 No.12

비부착 텐던을 갖는 원형 PSC 돔 구조물의 시간 의존적 해석

김운학,최정호 한경대학교 2009 論文集 Vol.41 No.-

This study presents analytical prediction of time-dependent characteristics and behaviors of the 1/4 prestressed concrete containment vessel with unbonded tendon considering time-dependent behavior. To represent the interaction between unbonded tendon and concrete, an unbonded tendon element based on the finite element method is developed using proposed unbonded tendon model. Some numerical examples of PSC structures with unbonded tendon are carried out using unbonded element and RC shell. The numerical results are verified by comparison with reliable experimental results. The crack and damage patterns also show good agreements with experiments

발열이 있는 호중구 감소증 환자에서의 위험도 예측인자

정중식,권운용,김규석,임용수,이중의,서길준,윤여규 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background: Most febrile neutropenic patients are treated in an aggressive manner. However, identification of low-risk patients may enable clinicians to administer risk-based treatment. The object of this study is to certify the factors associated with increased risk at the time of visiting the emergency department. Methods: This is a retrospective study. We reviewed the medical records of 101 febrile neutropenic patients who had visited the emergency department of Seoul National University Hospital from January 1998 to August 1999. We assumed 22 risk prediction factors that could be assessed at admission to the emergency department and 5 factors that could be assessed during treatment course. To find independent risk-prediction factors, we analyzed these factors respectively by using multiple regression analysis. Results: Tachycardia(aOR=136.5), altered mentality(aOR=28.8), decreased renal function(aOR=20.1), and significant comorbidity(aOR=17,2) are the independent factors associated with higher mortality. Altered mentality(aOR=31.6) and decreased renal function(CCr < 75㎖/min, aOR=5.4) are those associated with a higher incidence of septic shock. Independent factors associated with persistent(more than 3 days) fever are the early(within 10 days) onset of fever after last chemotherapy(aOR=8.8) and the existence of new pulmonary infiltrates on a simple chest X-ray(aOR=4.3). Conclusion: The stability of vital signs, the change of mentality, the renal function, the existence of significant comorbidity, the existence of new pulmonary infiltrates, and the rate of neutropenia are clinically useful risk-prediction factors in febrile neutropenia at the time of visiting the emergency department.

DNA Microarray를 이용한 인유두종 바이러스(Human Papilloma Virus:HPV)의 진단

정운원,이승관,이창규,조경진,김성욱 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.1

Human papillomavirus(HPV) has been known as one of the important pathogenic agent in uterine cervical carcinoma. The molecular works such as PCR enable the detection of large number of HPV genotypes obtained from viginal swab. Many of the PCR-based methods for HPV detection involve an amplification step followed by any of a number of methods for distinguishing different HPV types. In this study, we adopted the DNA chip technology enabling a HPV type-specific differentiation both low-risk group(type-6, 11, 34, 40, 42, 43, and 44) and high-risk group(type-16, 18, 31, 33, 35, 39, 45, 51,52,54, 56, and 58). MY09/MY11 and GP5+/GP6+primers covered LI region are used in nested PCR to improve PCR amplification. HPV type-specific probes for DNA chip were modified with NH2-C6, followed by spotting on silylated slides, washing slides and hybridization with each PCR products. Of 163 DNA samples chosen randomly, 42 samples were negative, 8 ones for low-risk group of HPV and 96 ones for high-risk group of HPV. Especially, co-infections with various HPVs were shown in 17 samples. A recent study found that multiple HPV is a factor in persistent HPV infection, resulting in the development of cervical dysplasia. This result emphasized the necessity to detect multiple HPV infection. The application of DNA-chip to determinate specific HPV typing will be a stronger candidate than any other PCR-based methods. Furthermore, the sequencing data of the positive PCR products were shown no discrepancy with DNA chip results. This means that DNA chip is very useful tool for both HPV detection and typing.