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김학준,김미정 경남대학교 환경문제연구소 1996 환경연구 Vol.18 No.-
Packed-bed electrode reactor is suitable for electrolysis of dilute solution such as electroplating wastewater, since each bed is consist of a number of particle electrodes. In this study, the effects of electrolysis time, circulation rate, temperature, cell current, accelerant on the electrolysis reaction are studied using a packed-bed electrode reactor. The cyanide decomposition efficiency is high when the circulation rate is 238 ㎖/min. The effect of the temperature shows that CN decomposition efficiency needs a high temperature for the good electrolysis reaction. The experiments were performed in order to insight the effect of cell current change on cyanide decomposition efficiency. They show that the lower current is prefered to the higher current. The experiments about accelerant show that NaOH(1000mg/ℓ) is more effective than NaOH(1000mg/ℓ). Under the optimum condition. decomposition efficiency of CN ion that is electrolyzed for 60 minutes at the ordinary temperature with NaOH(1000mg/ℓ) accelerant reaches as high as 86%.
GM peppers that are tolerant to the oriental tobacco budworm
Jung-Mi Park,Min Jung,Sun-Hee Shin,Eun-Mi Jeon,Soon-Ho Choi,Dong-Bo Shim,Beom-Seok Park,Chul-eui Jung,Chee Hark Harn 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Bt gene derived from the B. thuringiensis has been used for developing GM crops and those crops are already on the market. The aim of this study is to construct a genetic transformation with peppers using CryIAc1 gene and consequently to develop GM peppers resistant to the oriental tobacco budworm. We have developed transgenic peppers using Agrobacterium-mediated transformation, and obtained 5 T0 peppers. T0 peppers were self-crossed and T1 pepper fruits were exposed to larvae to test the survival rate. The survival rate of larvae that were fed with GM fruits decreased dramatically while with non-GM fruits the rate was not changed much. In order to establish the selection and the culturing of bug through a year, an incubating system for tobacco budworm in chamber was manufactured and a selection system under the media that are mixed with GM green pepper was obtained. Using those system, T3 peppers tolerant to tobacco budworm were selected.
Bae, Jung Myung,Ahn, Min Young,Harn, Chee Hark,Jeong, Won Joong,Jung, Min,Lim, Yong Pyo,Liu, Jang Ryol 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-
The plastid ribosomal protein s16 (rps16) gene was cloned from potato (Solanum tuberosum L. ssp. tuberosum cv De'sire'e) by PCR amplification to obtain a new homologous recombination site of plastid transformation. The potato rps16 genomic clone was 1627 bp in size and the coding region was interrupted by an 859 bp intron. Exon I was 40 bp, encoding 13 amino acids and exonⅡ was 227 bp, encoding a 76 amino acid polypeptide. The nucleotide sequence of the rps16 gene from the "De'sire'e" potato shared perfect identity with the sequence from the "Superior" potato in the coding region. Three nucleotide substitutions, two nucleotide insertions, and one nucleotide deletion were found between the intron sequence of both "De'sire'e" and "Superior" cultivars. The amino acid sequence of the potato rps16 gene showed a high level of identity with rice, maize, tobacco, and mustard (84-94%) and a relatively low level compared with Bacillus stearothermophilus and E. coli (27-28%). Expression of the rps16 gene was strong in chloroplasts and transcripts were detectable in amylopasts, suggesting that the rps16 gene is active in nonphotosynthetic plastids as well as in photosynthetic plastids. These results indicate that the potato rps16 gene can be used as a new homologous recombination site of plastid transformation for potato cultivars.
A new carrot germplasm constructed by protoplast fusion
Min Jung,Ji-Young Hyun,Young-Woo Liu,Chee Hark Harn 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
The most important factor in breeding program is to obtain the value-added genetic line. Generally, breeders develop genetic sources using several methods such as segregation-breeding, cross-breeding, backcross-breeding, mutation induction, tissue culture and so on. Here, we present one classical way but very valuable method called cell fusion or protoplast fusion to create genetic sources for the breeding practice. The method we developed was the asymmetric somatic-hybridization of protoplast isolated from carrots. This is rather to transfer the nucleus from the high quality F1 hybrid to other mediocre line to produce a new carrot line. Since the breeding a carrot line for higher quality and purity takes a long time, therefore this nuclear transfer technology is very beneficial to generate a new line that could be useful to breed elite varieties. We had obtained around 200 fused carrots (cybrids), 12 cybrids were self pollinated and produced seeds. Selected progenies are currently being evaluated for horticultural characteristics including self fertility.