Pseudomonas syringae pv. phaseolicola에 의한 에틸렌 생합성

이준승,Lee, June-Seung 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.3

식물의 고엽병균인 Pseudomonas syringae pv. phaseolicola에 의한 에틸렌 생성에 관하여 조사하였다. Krebs 회로 유기산이나 이와 관계있는 Asp, Glu 등의 아미노산은 이 세균에 의한 에틸렌의 생성에 효과적인 기질로 사용된다. 이 세균은 Asp를 기질로 했을 때 가장 많은 에틸렌을 지속적으로 생성하지만 Glu에 의한 에틸렌 생성은 3시간 후에는 거의 중단되었다. 유기산 중에는 Pyr>OAA>Mal>Fum>Succ>KG>Citr의 순으로 많은 양의 에틸렌을 생성하며, 5mM의 Arginine은 Pyr, OAA, Mal 등으로부터의 에틸렌 생성을 상승적으로 촉진시켜서 OAA+Arg나 Pyr+Arg에서는 Asp에서와 거의 같은 양의 에틸렌을 생성한다. Transaminase 억제제인 AOA, L-canaline이나, TCA 회로 억제제인 monofluoroacetate는 OAA나 Pyr로부터의 에틸렌 생성은 억제시키지 않으나 Asp, Glu나 다른 유기산으로부터의 에틸렌 생성은 억제시킨다. 이러한 결과로 보아서 OAA나 Pyruvate가 이 세균에 의한 에틸렌 생성의 가까운 전구체일 것으로 생각된다. Biosynthesis of ethylene by the Kudzu strain of Pseudomonas syringae pv. phaseolicola causing halo blight in Kidney bean plants was studied. Ethylene was effectively produced from the bacteria incubated with the Krebs cycle intermediates and related organic acids. Ethylene was continuously produced throughout a 6 h incubation period from Asp, OAA and KG supplied in the medium as substrates, but the rate of glutamic acid-based ethylene production decreased sharply following incubation for about 3 h Ethylene production induced by Succ, Fum, Mal, OAA and Pyr was further enhanced by arginine whereas citric acid- and KG-induced ethylene production was not affected. The rate of ethylene production with the Krebs cycle organic acids was in order of: Pyr>OAA>Mal>Fum>Succ>KG>Citr. Monofluoroacetate and, inhibitor of the Krebs cycle enzymes, and aminooxyacetate and L-canaline, both inhibitors of a PLP-linked enzyme, inhibited ethylene production induced by Glu and Asp, but not that induced by OAA and Pyruvate. The results obtained in this work suggest that either OAA or Pyruvate is a more direct precursor of bacterial ethylene production than any other organic acids tested.

식물세포에서 Ca²+ 의 생리적 역할

이준승 ( June Seung Lee ) 한국생화학분자생물학회 (구 한국생화학회) 1988 생화학총설집 Vol.2 No.-

당근 배양세포에서 RGD Peptide 가 에틸렌 생성에 미치는 영향

이준승(June Seung Lee),박정미(Jung Mi Park) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.4

It has been inferred that membrane-ECM (extracellular matrix) interaction in plants may be also mediated by an RGD-dependent recognition system as in animal cells. Effects of RGD peptide on ethylene production was examined in suspension cultured carrot cells. Treatment of the cells with RGD peptide containing RGD (Arg-Gly-Asp) sequence stimulated ethylene production. When RGD peptide was applied to carrot cells treated with IAA, the effect of RGD peptide appeared to be additive. ACC synthase activity in cells pretreated with RGD peptide likewise increased over the control. In an effort to check the sequence specificity of the RGD peptide, cells were treated with substituted RGD peptide, i.e. RGK (Arg-Gly-Lys) and RGE (Arg-Gly-Glu) peptide, respectively. RGK peptide did not stimulate ethlene production but RGE peptide did. The results strongly suggest that the stimulatory effect of RGD peptides on ethylene production may be associated with a physiological phenomenon through a specific recognition between RGD peptide including RGD sequence and their putative plasma membrane receptors.

개선형 한국표준원전 복합건물 인적, 오류, 방지, 개선 연구

June Seung Lee(이준승),Yoensub Jung(정연섭) 대한인간공학회 2010 대한인간공학회 학술대회논문집 Vol.2010 No.10

Objective: The aim of this study is to evaluate and to improve the alternatives of preventing human errors caused by insufficient distinction between two units side by side in the Korean Standard Nuclear Plant plus (KSNP+) that has a common compound building of the two units. Background: The equipment rooms of two units in the compound building of KSNP+ was arranged for line symmetry, so the possibility of human error from shortage of unit distinction has been raised. Method: We evaluated the alternative of human error prevention proposed at the stage of plant construction by research on the actual local condition, preference survey and setting an example on the spot. And then we searched the alternative of human error prevention during plant operation by benchmarking and administrative process review. Results: The method of color coding to improve unit distinction was revised from designer-centered to user-centered, and the 72 places to need color coding were founded. The 4 types of temporary extraneous matter and fixture to improve unit distinction were devised, and the 84 places to need them during plant operation were founded. Conclusion: The possibility of human errors in the compound building of KSNP+ could be minimized by color coding and the 4 types of extraneous matter and fixture to improve unit distinction. Application: The color coding was recently applied in the Shin-Kori 1 & 2 units, and the temporary extraneous matter and fixture will be applied.

Quercetin 이 보리 자엽초에서 옥신에 의해 유도되는 에틸렌 생성에 미치는 영향

이준승(June Seung Lee),곽정아(Jeong A Kwak) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.4

Effect of quercetin, a kind of natural plant flavonoids, on auxin-induced ethylene production in barley coleoptiles was studied. Auxin-induced ethylene production was apparently stimulated by quercetin. This stimulatory effect of quercetin appeared after 4 h of incubation period. Ethylene production was stimulated 200% over the control after 8 h of incubation by 3 × 10 exp (-5) M quercetin. The quercetin effect was most prominent at 10^-4 M of IAA. Ethylene production induced by the synthetic auxin, 2,4-D and NAA, was not significantly affected by quercetin. Also ACC-based ethylene production was unaffected by the flavonoid. In an effort to elucidate mechanisms of quercetin action on auxin-induced ethylene production, the effect of quercetin on IAA metabolism was studied. Data obtained from these experiments indicate that quercetin treatment resulted in about 90% inhibition of IAA oxidase activity. IAA ( 3×10 exp (-5) M) conjugation was found to be not affected by quercetin. This results suggest that the stimulatory effect of quercetin on auxin-induced ethylene production may be due to the fact that quercetin inhibits IAA oxidase activity, thus increasing the free IAA level.

Pseudomonas syringae pv . phaseolicola 에 의한 에틸렌 생합성

이준승 ( June Seung Lee ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.3

Biosynthesis of ethylene by the Kudzu strain of Pseudomonas syringae pv. phaseolicola causing halo blight in Kidney bean plants was studied. Ethylene was effectively produced from the bacteria incubated with the Krebs cycle intermediates and related organic acids. Ethylene was continuously produced throughout a 6 h incubation period from Asp, OAA and KG supplied in the medium as substrates, but the rate of glutamic acid-based ethylene production decreased sharply following incubation for about 3 h Ethylene production induced by Succ, Fum, Mal, OAA and Pyr was further enhanced by arginine whereas citric acid- and KG-induced ethylene production was not affected. The rate of ethylene production with the Krebs cycle organic acids was in order of: Pyr $gt; OAA$gt;Mal $gt; Fum$gt;Succ$gt;KG$gt;Citr. Monofluoroacetate and, inhibitor of the Krebs cycle enzymes, and aminooxyacetate and L-canaline, both inhibitors of a PLP-linked enzyme, inhibited ethylene production induced by Glu and Asp, but not that induced by OAA and Pyruvate. The results obtained in this work suggest that either OAA or Pyruvate is a more direct precursor of bacterial ethylene production than any other organic acids tested.

고리1호기 영구정지를 위한 주제어실 인간공학 확인 및 검증

이준승(Lee June-Seung),김용수(Kim Yong-Soo) 대한인간공학회 2018 대한인간공학회 학술대회논문집 Vol.2018 No.11

원전해체분야에서 인간공학기술의 적용

이준승(Lee June-Seung) 대한인간공학회 2019 대한인간공학회 학술대회논문집 Vol.2019 No.10

원전 사고관리 교육훈련용 증강 및 가상현실 기술 적용대상 선정

이준승(Lee June-Seung) 대한인간공학회 2019 대한인간공학회 학술대회논문집 Vol.2019 No.10

식물세포에서 분리한 Tonoplast Vesicle 에서의 Ca2+ 수송과 Inositol 1 , 4 , 5 - trisphosphate ( IP 3 ) 의 작용

김현주,이준승 ( Hyeon Joo Kim,June Seung Lee ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.4

Auxin may exert its effects through Ca^(2+) as a secondary messenger. To test this hypothesis, effects of auxin on Ca^(2+) release from isolated tonoplast vesicles was investigated. Tonoplast vesicles were isolated from red beet roots(Beta vulgaris L.) by metrizamide discontinuous density-gradient centrifugation. Uptake of ^(45)Ca^(2+) into tonoplast vesicles was ATP-dependent, and was blocked by calcium entry blockers such as Diltiazem, Verapamil, Flunarizin. Auxin did not induce the release of ^(45)Ca^(2+) from tonoplast vesicles preloaded with the cations, but Inositol 1,4,5-trisphosphate (IP₃) did. The IP₃ induced ^(45)Ca^(2+) release was dependent on IP₃ concentration, and was blocked by the Ca^(2+) antagonist, 8-(N,N-diethylamino)-octyl 3,4,5-trimethoxybenzoate-HCl (TMB-8). Auxin is known to generate transient change in levels of Inositol 1,4,5-trisphosphate (IP₃) and Inositol bis-phosphate (ptdIns(4,5)P₂) within minutes in Catharanthus roseus (Ettlinger, et al., 1988). These results indicate that auxin cannot alter cytoplasmic calcium levels directly but through the IP₃ secondary messenger system.