Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang, Chul Hak,Jun, Gyo,Lee, Dug Keun,Lee, Sang Kyou,Park, Se Yeon,Hahm, Eun Ryeong 생화학분자생물학회 1998 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the conventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

충남대학교 학술림 산책로 훼손실태 및 관리방안에 관한 연구

이준우,김명준,최윤호,전용준,오도교,김민호,이소연,이경형 충남대학교 농업과학연구소 2004 농업과학연구 Vol.31 No.2

This study concentrates an the trail of the CNU(Chungnam National University) forest. The amount of use and the pattern of damage are considered, as well as the extent of environmental damage. Finally, some managerial strategies are proposed in connection with sustainable maintenance. The trail of the university forest is composed of three sections; one is the neighborhood park and the Institute of Information Technology/Communications, the second is the Institute of Information Technology/Communications and the Dormitory, and the third section is the Dormitory and the Foreign prof's Apt. The total length of the trail is 2.6km. The daily amount of use is 1,269 visitors per day. The amount of using increased three times the amount of that in 1998. This study shows the pattern of damage of the trail in the order of root-exposure, widening, rock-exposure, deepening and the divergence in the frequency of occurrence of all these factors. Environmental damage is also graded thus: grade 3 is 27.4m, grade 4 is 0.8m, grade 5 is 1.5m, and grade 6 is 0.2m. Accordingly, some managerial strategies were proposed in connection with the sustainable trail maintenance.

벤치컷으로 굴착되는 터널의 해석에 적합한 하중분배율의 산정

박연준 水原大學校 2012 論文集 Vol.26 No.-

Load distribution factors suitable for the analyses of tunnels excavated by bench cut were determined. To achieve this, 3-D analyses were conducted while taking every construction stage into account. Then 2-D analyses were conducted which yield the same results with the 3-D results. The crown settlement normalized by the ultimate value was compared during the process to overcome the discrepancy caused by difference in dimension. When the length of the bench is very short compared to the diameter of the tunnel, in such cases as in short bench cut or in mini-bench cut, the supporting effect of the face does not disappear even after the bench is completely excavated since the face is still too close to the point of interest. The 4th load distribution factor was defined to stand for the advance of the face after the completion of the excavation cycle. The 4th load distribution factor turned out to be very useful in analyzing the behavior of tunnels excavated by bench cut with various bench lengths.

내용과 형식 스키마가 독해에 미치는 영향

연준흠 한국영어어문교육학회 1997 영어어문교육 Vol.3 No.-

The purpose of this research was to investigate the effects of content and formal schema on reading comprehension. Five hundred fiftynine subjects from high school were assigned to one of the following levels and treatment conditions : (1) Higher level & Schema Activation, (2) Higher level & Non-schema Activation, (3) Lower level & Schema Activation, and (4) Lower level & Non-schema Activation. To evaluate the effects of schema activation, two experiments were conducted : one was related to the content schema and the other to the formal schema. To evaluate the effects of content schema, three different types of tests were conducted : (1) cloze test, (2) guessing the meanings of nonsense words, and (3) immediate recall test. To evaluate the effects of formal schema instruction, four kinds of tests were conducted : (1) sorting the sentences according to the importance, (2) identifying the signal words, (3) immediate recall test, and (4) identifying the specific information. For content schema condition, results indicated that the subjects given the titles or pictures before reading in "Content Schema Activation" treatment had better grades than those of the other treatment in all types of tests, regardless of their levels. Schema activation helped the subjects to increase the cognitive predictability of missing words and to participate in the tasks more actively with risk-taking. And it was also shown that good readers tend to process the words meaningfully, while poor readers tend to process the words phonetically or morphologically. Formal schema activation through teaching the text organization also had a significant influence on three types of tests : sorting the sentences according to the importance, identifying the signal words, and immediate recall test, but not on identifying the specific information. The implications from this study can be briefly noted as follows : (1) In teaching reading, the student's background knowledge should be activated as a pre-reading activity. (2) In reading, it is more important to emphasize the student's schema than the features of the text. (3) Various educational interventions should be introduced, especially for the lower level students. (4) Teaching text structures can be a powerful method for the top-down processing strategy.

소집단 학습자 구성 방법에 따른 체육 학습 효과

하영준,김연갑 인하대학교 스포츠과학연구소 2000 論文集 Vol.12 No.-

Today, the learning with small groups is a popular style of pedagogy in the field of so called "open education" which is being concerned in the elementary and middle school. Despite many researches on the effects on learning with small groups had been implemented, there has not been any consensus of decision yet, which is the most important factor in .the pedagogy with small groups, Therefore the main purpose of this study is to find the way to organize the small groups which is the most essential part in learning with small groups, comparing the effects on physical learning of the elementary school student by the way of forming small groups(homogeneous and heterogeneous groups), in the field of physical teaching-learning, which is one of the learning styles in small groups, coming from "Spectrum of teaching style" developed by Mosston. For the experimentation, 95 students(50 bys and 45 girls) in their forth grade are selected from 2 classes in "C" elementary school located in In-Cheon and are divided into 2 groups, that is to say, homogeneous(small group by each levels) and heterogeneous(small group by mixed levels) group. Then these two sample groups are instructed to dribble and pass the ball in the simple basketball and soccer game respectively, three times a week, through the regular physical class for 12 weeks. Meanwhile a pre-test is implemented in the first second week and final test in the eleventh twelfth week. The following results can be achieved from this research; First, there was no big difference between the homogeneous and heterogeneous group, once the same contents and circumstances had been provided to the small group.(P>0.05) Second, organizing homogeneous and heterogeneous group from the whole group made no big different effects in learning(P>0.05), however, as a result of comparing the learning effects on the boy group and girl group by levels(high, intermediate, low), significant difference was observed in the high level soccer group of boy(P>0.05) and dribbling and passing group of girl(P>0.05), and no significant difference was caught from the remaining learning effects by the event, gender and small groups(high, intermediate, low) Therefore, the average comparison showed that homogeneous group in the high level students, heterogeneous in the intermediate and low are tend to get better learning effects. Consequently, the above-mentioned research makes a meaningful way to the efficient management on the learning with small group of the "open education" which is most concerned recently. Especially, it shows that the learning ability of the student, the characteristics of the learning subject and the external conditions for the class are the main factor in the way of forming groups and gives a hint of necessity of the flexible grouping to the things given. It will also be helpful to form small groups in the course by level, which is the basis of seventh education course.

Morgagni 탈장 : 1례 보고

오연희,이창욱,김승현,이성우,정병욱,이준희,박동일,권영무,신현종 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

Morgagni 탈장은 횡격막의 전내측 부위의 발달이상으로 열공을 통하여 장관이나 대망과 같은 복강내 구조물이 흉곽내로 빠져나가는 드문 질환으로 알려져 있다. 저자들은 호흡곤란을 주소로 내원하여 방사선학적 검사와 수술로 확진된 1례를 보고한다. Morgagni hernia is improperly located abdominal viscera into the thoracic cavity through the foramen of Morgagni which is a congenital defect at the anterornedial portion of diaphragm. We report a case of Morgagni hernia diagnosed by radiologic examination and operation.

대불공과대학교내 지하수의 음용수적 측면에서 본 환경적 연구

안연준 대불대학교 1995 論文集 Vol.1 No.2

The 39 items were examined four times about the groundwater at Daebul University 72 beongi, san-ho ri, sam-ho myun, young-arm gun, jeollanam-do, Korea. That items have 4 categories, that are microbes, harmful minerals and organic substances, and esthetic factors. Sampled groundwaters had been satisfied that 4 categories. But the ecological conditions of the Daebul university are very rapidly changes, which have a contamination possibility. So, we have to scane the groundwater of Daebul University.

한국인 HIV 감염자에서 항레트로바이러스 치료 이행도와 바이러스학적 치료 실패의 상관관계

최준용,박윤수,김창오,박윤선,윤희정,신소연,김연아,송영구,김준명 대한감염학회 2005 감염과 화학요법 Vol.37 No.1

목적 : HIV 감염자에서 항레트로바이러스 치료에 대한 바이러스학적 치료실패와 연관된 인자들로는 낮은 CD4 양성 T 림프구, 낮은 치료 약물 농도. 잘못된 처방등이 있는데, 이들 중에서 낮은 치료 이행도는 바이러스학적 치료실패의 대표적인 원인 중 하나이다. 국내 HIV 감염자를 대상으로 HAART 이행도를 평가하고 낮은 이행도와 관련된 인자를 알아보고 치료 이행도와 바이러스학적 치료실패와의 연관성을 규명하고자 하였다. 재료 및 방법 : 연세대학교 의과대학 세브란스 병원에 내원한 한국인 HIV 감염자 중 HAART를 시행 받고 있는 환자들을 대상으로 하였다. 환자들의 HAART 이행도를 평가하기 위한 6가지 문항의 설문을 수행하였다. 설문에 의해 측정한 HAART 이행도에 영향을 미치는 요인들을 조사하였고, 낮은 이행도와 바이러스학적 치료실패 간의 상관관계를 조사하였다. 결과 : 총 49명의 HIV 감염자가 설문에 응하였다. 대상 환자의 53.1% 만이 약 먹는 것을 잊은 적이 전혀 없었다고 응답하였고, 지난 1주일간 약을 모두 복용하였던 환자는 대상 환자의 46.9%였다. 약을 복용하지 않은 이유에 대해 32명이 응답하였는데, 별다른 이유 없이 잊어버려서 약을 복용하지 않았다고 응답한 경우가 14명으로 가장 많았고, 부작용 때문이거나 바빠서 약을 복용하지 않았던 경우가 각각 5명으로 나타났다. HIV 진단 이후 경과한 시간이 길수록, HAART를 시행한 기간이 길수록 이행도가 유의하게 낮은 것으로 나타났으며, CD4 양성 T림프구수가 낮을수록 이행도가 낮은 것으로 나타났다. 설문으로 평가한 HAART 이행도가 바이러스학적 치료실패와 유의한 상관 관계가 있었다(P<0.05). 결론 : 낮은 치료 이행도는 항레트로바이러스 치료의 실패를 가져올 수 있으므로 치료 이행도를 지속적으로 평가하고 향상시키는 것은 HIV 감염의 성공적인 치료를 위하여 매우 중요하다. 향후 국내 HIV 감염자에서 치료이행도를 평가하기 위한 다양한 방법이 개발되어야 하겠고, 치료 이행도를 높이기 위한 다각적인 전략이 마련되어야 하겠다. Background : Several factors associated with human virologic failure in patients with immunodeficiency virus (HIV) infection are low CD4+ cell counts, advanced clinical stage, poor subscribing, subtherapeutic drug levels, etc. Among these factors, poor adherence is one of the leading causes. Materials and Methods : We evaluated the adherence to antiretroviral treatment in HIV infected Koreans using questionnaire. We evaluated the factors associated with poor adherence and analyzed the correlation between the adherence to antiretroviral treatment and virologic failure. Results : A total of 49 patients responded to the questionnaire. Of the 49 patients, 26 (53.1%) answered that they had never forgotten to take their medicine, and 23 (46.9%) answered that they had taken all their medications during the last week. The reasons for missed doses were forgetting, being too busy, having adverse side effects, etc. Low CD4+ T cell counts, longer duration of antiretroviral treatment, and longer duration of being diagnosed with HIV infection were associated with poor adherence in HIV infected Koreans (P<0.05). There was correlation between poor adherence and virologic failure in these HIV-infected Koreans (P<0.05). Conclusion : Because poor adherence induces treatment failure, monitoring adherence is very important for successful antiretroviral treatment. Questionnaire was a good method for monitoring adherence in HIV infected patients.

SCF를 이용한 시간지연 회로의 설계 및 제작기술 개발

황준원,박종연,장목순 江原大學校 産業技術硏究所 1996 産業技術硏究 Vol.16 No.-

This paper deals with the tapped time delay circuit with SCF(Swiched Capacitor Filters). This filter is composed fo lossless discrete integrator and the SCF has 2-phase clocks. Experimental results have shown that telephone signals (0~4kHz) could be delayed in the range of sampling frequency 80kHz.But above the range, operational amplifiers and analog switchs have been difficult in the normal operating condition