A Quick Query Methods for ONS System based on EPC

Jun Bai,Qiang Zhu,Bai-ling Wang,Liu Yang,Qiang Chen 보안공학연구지원센터 2013 International Journal of Hybrid Information Techno Vol.6 No.6

Organization process of the hierarchical structures in microbially synthesized polyhydroxyalkanoates

Jun Xu,Bao-Hua Guo,Qiong Wu,Jin-Chun Chen,Guo-Qiang Chen,Jian-Jun Zhou,Yong Jiang,Lin Li 한국물리학회 2007 Current Applied Physics Vol.7 No.s1

cess of the high-order structures in biomaterials. Real-time Fourier transform infrared spectroscopy and optical microscopy demon-strated that helical segments formed along with the spherulite growth. Atomic force microscopy revealed the details of growth,twisting and branching of lamellar crystals. Cooperative packing of these twisting lamellae led to regular banded spherulites observedunder polarized light microscopy. Real-time observation on the crystallization process provided richer information than the characterization of the final structures; consequently, it provides deeper insight into the organization mechanism of the hierarchical structures.

Proanthocyanidins accelerate the germination of cucumber (Cucumis sativus L.) seeds

Li-jun Zhu,Xing-guang Deng,Li-juan Zou,Jun-qiang Wu,Da-wei Zhang,Honghui Lin 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

Proanthocyanidins (PAs) are the end products of the flavonoid biosynthetic pathway in many seeds, but their biological function is rarely unknown during seed germination. In the present study, we observed that PAs pretreatment accelerated cucumber seeds germination with maximum efficiency at 0.15% by measuring germination percentage and radical length. Using inhibitors of abscisic acid (ABA), gibberellins (GA) and alternative oxidase (AOX) and H2O2 scavenger pretreatment and gene expression analysis, we found that the accelerated effect of 0.15% PAs on seed germination was due to the decreased ABA biogenesis and enhanced GA production. ROS are induced by PAs pretreatment. Then, the enhanced ROS contributed to GA and ethylene accumulation and ABA decrease in seeds. Moreover, the improvement of GA was involved in the further induction of antioxidant enzymes activities. Therefore, our findings uncover a novel role of PAs in seed germination and clarify the relationships between ROS, ABA, GA and ethylene during seed germination.

중국진출 한국기업의 해외직접투자 성과 결정요인에 관한 연구

왕군강(Jun-Qiang Wang),권영철(Yung-Chul Kwon) 한국관세학회 2014 관세학회지 Vol.15 No.2

The purpose of this study is to assess the determinants of Korea FDIs performance in China. To achieve this purpose we adopt Dunnings Eclectic theory(1980) which compromises the classical FDI theories such as monopolistic advantage theory, internalization theory and location advantage theory. Based on the analysis of 152 Korea companies operating in China, it was found that both monopolistic advantages and location advantages have significant effects on Korea FDIs performance in China. This result suggests that Korea firms should have possess the monopolistic advantages such as technology and brand to secure FDIs performance and should choose locations that have the advantages in terms of production costs and market potentials. In addition, it was also found that under the circumstances of monopolistic advantages and location advantages, Korea firms have better FDIs performance when entering the Chinese market by using sole ventures over joint ventures. This result suggests that Korea firms should internalize their monopolistic and location advantages to secure FDIs performance.

Comparative study of the microstructure of Ti-6Al-4V titanium alloy sheets under quasi-static and high-velocity bulging

Fen-Qiang Li,Jun Zhao,Jian-Hua Mo,Jian-Jun Li,Liang Huang 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.3

In order to reveal the high-velocity deformation mechanisms of Ti-6Al-4V titanium alloy sheets, the dynamic deformation behavior and the microstructure evolutions were compared with those under quasi-static case employing Scanning electron microscopy/Electron back-scattered diffraction (SEM/EBSD) and Transmission electron microscopy (TEM). The results indicated that the distribution of microhardness was uneven. The cause was determined to be the non-uniform material flow and dynamic loading. The grain sizes of the Ti-6Al-4V titanium alloy sheet were nearly the same under two conditions, but the grains were elongated under the dynamic loading compared with that observed in quasi-static forming. In high-velocity bulging, dislocation slip was the principle mechanism of plastic deformation of Ti-6Al-4V titanium alloy when deformation was small. As the strain increased, the twinning phenomenon was activated in addition to the dislocation cell structure. The twinning plane was determined to be the (10 11) plane.

Effect of cadmium exposure on antioxidant enzyme catalase in different tissues of Acrossocheilus fasciatus

Yong-Qiang Zhao,Guo-Di Liu,Cong-Cong Hou,Ying-Li Han,Jun-Quan Zhu,Jun-Quan Zhu 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.3

Catalase (CAT) is an essential antioxidant in organisms, its can eliminate hydrogen peroxides produced in the cellular environment. Previous studies have focused mainly on the functions of CAT by pathogen challenge, but the functions of CAT by metals stress remain unclear. In order to investigate the function of CAT in Acrossocheilus fasciatus (AfCat), we cloned CAT complete cDNA sequences and analyzed its functions by cadmium (Cd) stress. qPCR analysis illustrated that the expression of CAT changed rapidly and dynamically in response to Cd from 0.05 mg L-1 to 1.25 mg L-1. Enzyme activity assay result showed that CAT activity was up-regulated significantly in response to Cd from 0.05 mg L-1 to 1.25 mg L-1. Histochemistry analysis showed that the structure of liver, testis and kidney were severely damaged by exposed to Cd of 1.25 mg L-1. Collectively, the results of our study indicate that AfCat may play an indispensable role in maintaining redox balance under Cd exposure, but AfCat expression will be inhibited when Cd accumulation reaches to the critical concentration. This study will illuminate the potential functions of AfCat in the defense mechanisms of reproductive and metabolic systems under Cd exposure in A. fasciatus.

Optimized Transformation of Streptomyces sp. ATCC 39366 Producing Leptomycin by Electroporation

Yong-Qiang Fan,Hong-Jian Liu,Li Yan,Yu-Shi Luan,Hai-Meng Zhou,Jun-Mo Yang,Shang-Jun Yin,Yu-Long Wang 한국미생물학회 2013 The journal of microbiology Vol.51 No.3

Streptomyces sp. ATCC 39366 produces leptomycin derivatives. Leptomycin B, a potent and specific inhibitor against the export of nuclear proteins, is the main product; however, the introduction of DNA into this strain is almost impossible, which has impeded its further use. We developed a Streptomyces sp. ATCC 39366 transformation protocol to introduce foreign DNA via electroporation. Various conditions were examined, including treatments of the cell wall with weakening agents, electroporation parameters, and DNA content. We found that only plasmid DNA isolated from a dam- ET12567 strain resulted in successful transformation. The mycelium growing in a yeast-peptone-dextrose medium supplemented with 1% glycine at 28°C on a rotary shaker (220 rpm) was more dispersed than those without supplementation and prone to electroporation. The maximum transformation efficiency of 8×102 CFU/μg plasmid DNA was obtained at a field strength of 13 kV/cm with a time constant of 13 ms (25-μF capacitor; parallel resistance, 600 Ω) using 1-mm electrocuvettes. The results of the transformations of two other Streptomyces species indicated that the optimized conditions established in this study might only be applicable to Streptomyces sp. ATCC 39366. However, this is the first report of successful transformation of Streptomyces sp. ATCC 39366, and will facilitate the construction of a gene knockout mutant in Streptomyces sp. ATCC 39366 to produce series of new leptomycin derivatives.

Expression of Connexin 43 and E-cadherin Protein and mRNA in Non-small Cell Lung Cancers in Chinese Patients

Zhao, Jun-Qiang,Sun, Fang-Jie,Liu, Shan-Shan,Yang, Jun,Wu, Yu-Quan,Li, Gui-Shan,Chen, Qing-Yong,Wang, Jia-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Aim: Connexin 43 (Cx43) and E-cadherin are important biomarkers related with cancer. Their expression at protein and mRNA levels was here investigated in 50 primary lung carcinoma tissues and 20 samples of adjacent normal tissue of Chinese patients with non-small cell lung cancer (NSCLC). Methods: Protein and mRNA expression were evaluated by ABC immunohistochemistry and RT-PCR. Results: (1) The positive expression rates of Cx43 and E-cadherin protein were higher in the adjacent normal tissues than those in the primary lung carcinoma tissues; (2) the positive expression rates of Cx43 and E-cadherin protein decreased with NSCLC progression; (3) the expression of E-cadherin protein was not related with the pathological type of NSCLC; and (4) the relative quantity of the Cx43 or E-cadherin mRNA expression was correlated with the the histological type, clinical stage, cancer cell differentiation and the lymph node metastasis. Conclusion: The data suggested that the Cx43 and E-cadherin are reduced with NSCLC progression, and might be important biomarkers for judging the metastasis and prognosis.

Celastrol, produced by Tripterygium wilfordii Hook F. enhances defense response in cucumber seedlings against diverse environmental stresses

Li-jun Zhu,Xing-guang Deng,Li-juan Zou,Peng-xu Li,Jun-qiang Wu,Da-wei Zhang,Honghui Lin 한국식물학회 2017 Journal of Plant Biology Vol.60 No.1

Celastrol is an active triterpenoid compound isolated from Tripterygium wilfordii Hook F.. Many reports have highlighted that celastrol is an effective, safe and desirable approach to the treatment of cancers. However, their biological function during environmental stresses in plants is rarely reported. In the present study, the effects of celastrol on the tolerance against high light (HL), polyethylene glycol (PEG) and cold stress in cucumber (Cucumis sativus L.) were investigated. Celastrol pretreatment could enhance cucumber seedlings stress tolerance at a concentration of 1 μg ml–1. The results showed that pretreatment with 1 μg ml–1 celastrol clearly induced the activities of antioxidative enzymes, which subsequently alleviated stress-induced oxidative damage in plant cells. We also provided evidence that celastrol upregulated ABA biosynthetic gene NCED2 expression and ABA accumulation in cucumber seedlings, which resulted to the enhanced tolerance in response to environmental stresses. Furthermore, the celastrol-pretreated seedlings showed less photosystem damaged caused by the stress conditions, when compared with the control. Therefore, our findings provide a novel role of celastrol in plant against environmental stresses and indicate that the celastrol-induced activities of antioxidative enzymes and ABA content might contribute to the stress tolerance.

Effects of astaxanthin on antioxidant capacity of golden pompano (Trachinotus ovatus) in vivo and in vitro

( Jia-jun Xie ),( Xu Chen ),( Jin Niu ),( Jun Wang ),( Yun Wang ),( Qiang-qiang Liu ) 한국수산과학회 2017 Fisheries and Aquatic Sciences Vol.20 No.2

The objective of this research was to study the effect of astaxanthin (AST) on growth performance and antioxidant capacity in golden pompano (Trachinotus ovatus) both in vivo and in vitro. In the in vivo study, two diets were formulated with or without astaxanthin supplementation (D1 and D2; 0 and 200 mg/kg) to feed fish for 6 weeks. In the in vitro study, cells from hepatopancreas of golden pompano were isolated and four treatments with or without astaxanthin and H₂O₂ supplementation were applied (control group: without both astaxanthin and H₂O₂ treated; H₂O₂ group: just with H₂O₂ treated; H₂O₂ + AST group: with both astaxanthin and H₂O₂ treated; AST group: just with AST treated). Results of the in vivo study showed that weight gain (WG) and special growth rate (SGR) significantly increased with astaxanthin supplemented (P < 0.05). Feed conversion ratio (FCR) of fish fed D2 diet was significantly lower than that of fish fed D1 diet (P < 0.05). Hepatic total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of golden pompano fed D2 diet were significant higher than those of fish fed D1 diet (P < 0.05). Superoxide dismutase (SOD) was significantly declined as astaxanthin was supplemented (P < 0.05). Results of the in vitro study showed that the cell viability of H₂O₂ group was 52.37% compared to the control group, and it was significantly elevated to 84.18% by astaxanthin supplementation (H₂O₂ + AST group) (P < 0.05). The total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of cell were significant decreased by oxidative stress from H₂O₂ (P < 0.05), but it could be raised by astaxanthin supplementation (H₂O₂ vs H₂O₂ + AST), and the malondialdehyde (MDA) was significant higher in H₂O₂ group (P < 0.05) and astaxanthin supplementation could alleviate the cells from lipid peroxidation injury. In conclusion, dietary astaxanthin supplementation can improve the growth performance of golden pompano. Moreover, astaxanthin can improve the golden pompano hepatic antioxidant capacity both in vivo and in vitro study by eliminating the reactive oxygen species.