The effect of neuropeptides on secretion of Interleukin-8(IL-8)

Kim, Kyung-Jun,Park, Sang-Hyuk,Choi, Kyoung-Kyu,Park, Sang-Jin 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.3

본 연구는 치수조직, 치은, 치주인대로부터 배양된 조직을 SP (Substance P)로 4시간, SP, CGRP (Calcitonin Gene-related Peptide), Tumor necrosis factor-α (TNF-α)로 8시간 자극 후 RNase Protection Assay를 시행하고, IL-8의 분비량을 측정해 다음 결과를 얻었다. 1. IL-8 mRNA는 모든세포에서 발현됐다. 2. IL-8 mRNA 발현은 SP (10^(-5)M)와 SP (10^(-8)M)로 4시간 자극 시 증가되지 않았다. 3. IL-8 mRNA 발현은 SP (10^(-4)M)와 CGRP (10^(-6)M)로 8시간 자극 시 증가되지 않았다. 4. TNF-α (2 ng/㎖) 자극 시, IL-8 mRNA 발현이 증가됐다. 5. 치은 세포를 CGRP (10^(-6)M)로 8시간 자극 시, IL-8 분비량이 증가했다 (p < 0.05). 6. 치주인대 세포를 SP (10^(-4)M)로 8시간 자극 시 IL-8 분비량이 증가했다 (p < 0.05). We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted instact 3rd molars. Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05. According to this study, the results were as follows: 1. IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF). 2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10^(-5)M) and SP (10^(-8)M) compared with Mock stimulation in all type of cells studied. 3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10^(-4)M) and CGRP (10^(-6)M) compared with Mock stimulation in all type of cells studied. 4. TNF-α (2 ng/㎖) increased the expression of IL-8 mRNA in all kind of cells studied. 5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10^(-6)M) (p < 0.05). 6. The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10^(-4)M) (p < 0.05). Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

전통시장 시설현대화사업이 전통시장 활성화에 미치는 영향 분석

이준호(Lee, Jun-Ho),김영(Kim, Yeong),김경훈(Kim, Kyoung-hun) 한국지역개발학회 2013 韓國地域開發學會誌 Vol.25 No.1

After full opening of distribution market in 1996, its competitiveness was lost and declined due to the emergence of a variety of distribution types, change of customers consumption habits, and purchase patterns. Therefore, Korean government began to support the modernization projects of traditional markets through the establishment of law for the activation of existing traditional markets. In this regard, this study attempts to diagnose the problems of facility modernization projects through substantial and concrete effect analysis on facility modernization projects to provide the activation methods effectively. To achieve this goal, we analyzed the overall satisfaction and substantial effect of facility modernization projects for traditional markes by carrying out questionnaire surveys on merchants at Jun-gang Yudeung Market in Jinju. As a result of questionnaire survey, customers were generally satisfied with the facility modernization project, the market environment was improved and the customer numbers and sales amount which show the actual improvement also increased after implementing the projects. Also, it was proved that customer numbers and the sales amount after the facility modernization project of traditional markets increased and the degree in the increase was low level of 5~20%. Therefore, it was analyzed that sub-project items of the facility modernization project, which have a significant effect on the activation of traditional markets, had a low level of influence.

농산물 유통체계 변화와 생산자 대응방안

전경수,박상현 진주산업대학교 농업기술연구소 2001 農業技術硏究所報 Vol.14 No.-

Nowadays, the agricultural products trade system is changing rapidly because the condition of farming trade system is undergoing a change quickly. Since 1996 trade market was opened completely, so many multinational foreign enterprises are advancing our domestic market. These new trade enterprises are looking for marketing strategy that treat agricultural products strategically. So whole agricultural products system are changing fast. Finally it affects not only trade but also production parts. And then I'll analyze the participation condition of products trade according to the agricultural products marketing changes and its changing prospect. Moreover the countermeasure has to have trademind as a producer of products, and recognize the stability of goods to satisfy the standard of consumer. At last, producer community should develop business positively to raise the power of the marketing negotiation and should have a information about the trade change.

조직공학적 연골생성에 있어서 연골막의 필요성

오경석,김해진,이동준,정재호,최준혁 大韓成形外科學會 2001 Archives of Plastic Surgery Vol.28 No.4

평면에서 형상정보를 갖는 물체의 접촉 해석

전경진,손정현,강동권 동의공업대학 2001 論文集 Vol.27 No.1

This paper presents a method for calculating contact force of any shaped rigid bodies on plane. Every integration time step, the proposed method finds expected contact point on their outlines and then calculates penetration and contact force. This paper is using the continuous analysis method to calculate contact force. To get the accurate expected contact point on their outlines the new algorithm is developed. Through the comparison with DADS, we can prove the accuracy of the proposed algorithm.

축구 활동이 비만 초등학생의 혈중 지질에 미치는 영향

전태원,박익렬,엄우섭,이동기,우재홍,김경래,김은경,서한교,전병환,김광준,정진욱,박성태 서울대학교 체육연구소 2002 서울大學校 體育硏究所論集 Vol.23 No.2

The purpose of this study is to investigate the effect of Soccer playing in Plasma Lipids in Elementary Obesity Student. Subject for this study were consisted of 16 male(exercise group : 8, non-exercise group : 8), who were over 25% of body fat. Exercise group played soccer 3 times per week, one time was 90 minutes. Weight, % body fat, total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, triglyceride, c-ratio were measured at three time periods before exercise begin, and 5 and 8 weeks. The results were summarized as follow: Exercise group decreased significantly more than non-exercise group about weight, % body fat, total cholesterol, low density lipoprotein cholesterol, triglyceride, c-ratio. And Exercise group increased significantly more than non-exercise group about high density lipoprotein cholesterol. In conclusion, the regular aerobic exercise(soccer playing) for 10 weeks has great effects on control of serum lipid which is related to the increase well-being(reduced coronary heart disease, hyperlipidemia, hypertension, obesity et all.) in elementary obesity male student.

정신과에서의 뇌파

전태연,양완석,이경욱,채정호 한국의학사 2004 診斷과治療 Vol.24 No.1

양극성 장애 1형에서 종양괴사인자 베타 유전자 다형성

전태연,이경욱,배치운,김원,우영섭,채정호,박원명 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : Bipolar disorder is known to have a high genetic predisposition. Recently, the main focus of etiologic studies in bipolar disorder has been concentrated on molecular genetic approach including gene polymorphism analysis. The present study was conducted to investigate whether TNFB polymorphism is associated with bipolar I disorder in the Korean population. Methods : 89 bipolar I disorder patients diagnosed by DSM-IV criteria were assigned as the patient group and 202 normal population, matched on age and sex from Catholic hemopoietic stem cell bank (Seoul, Korea), were enrolled as the control group in this study. Genotyping was performed by a polymerase chain reaction-restriction fragment length polymorphism method. All data was analyzed by x² test. Results : There were no significant differences in frequency of TNFB*1/1,TNFB*1/2 and TNFB*2/2 between bipolar I disorder patient group and normal control group. The frequency of TNFB*1 was not statistically different between bipolar I disorder patient group and normal control group. Conclusion : The difference of frequency in TNFB*1/TNFB*2 gene between the bipolar I disorder gropup and the normal contro1 could not be verified. The present result suggested that the gene polymorphism of TNFB may not play a significant role in susceptibility to bipolar I disorder. Studies with a larger number of subjects from different ethnic backgrounds, considring clinical phenotype and controlling various factors, should be launched to further determine the role of TNFB in bipolar I disorder.

동합금의 생물학적 친화성에 대한 연구

김경남,박경준,이서영 大韓齒科器材學會 1991 대한치과재료학회지 Vol.18 No.2

The rise in the cost of gold was lead to use base metal alloy instead of using gold alloy which was known to be most biocompatible. But these base metal alloys are too hard and have low biocompatibility compared to gold alloy. Especially, nickel allergy & hypersensitivity came to be a problem, so use of these alloys is needed to take a careful consideration. In addition, copper alloy has been used in dentistry until now because of giving a golden color but it has lower castability and higher solubility in the oral environment compared to gold alloy. Many reports also have been made on the high toxicity of copper alloy, which revealed the inadequate dental uses of copper alloy. Recently, NPG copper alloy (Alba Dent Co., U.S.A.) have been introduced in Korea. This study was done cell toxicity test (Agar overlay test) and tarnish test in artificial saliva for 30 days and in 5% Na₂S solution for 13 days for 3 gold alloys, a Ag-Pd alloy, and NPG copper alloy. The results were as followed; 1. NPG copper alloy indicated zone index 3 and lysis index 5 in agar overlay test, which revealed high toxicity compared to gold or silver alloys. 2. In tarnish test, contrast to a small color change of gold and silver alloys, NPG copper alloy had a striking color change, 1st day in artificial saliva indicated 2 degree, 30th day in artificial saliva indicated 4 degree and in 5% Na₂S solution, extreme color change of 5 degree showed even on the 1st day.

관상동맥 스텐트 내 재협착의 발생에 미치는 임상적 관동맥 조영술적 요인들

윤혁준,김기식,박형섭,이영수,현대우,한성욱,허승호,김윤년,김권배,박남희,원경숙 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.12