Mitogenic Signal Transduction by Integrin- and Growth Factor Receptor-mediated Pathways

Rudy Juliano,이정원 한국분자세포생물학회 2004 Molecules and cells Vol.17 No.2

Engagement of cells with the extracellular matrix (ECM) proteins is crucial for various biological proc-esses, including cell adhesion, spreading, proliferation, differentiation, migration, apoptosis, and gene induc-tion, contributing to maintenance of tissue integrity, embryogenesis, wound healing, and the metastasis of tumor cells (Hynes, 2002b; Juliano, 2002). The en-gagement involves cell adhesion mediated by integrins, a large family of cell adhesion receptors that are transmembrane glycoproteins which bind to ECM or to counter-receptors on neighbor cells. In this review, the molecular basis of signaling mediated by integrins and their collaboration with growth factor receptors will be discussed, based on recent observations. Although other cell adhesion receptors including cadherins, se-lectins, syndecans, and the immunoglobulin superfamily of cell adhesion molecules (IgCAMs) can play important roles or be involved in these processes, we suggest read-ers refer to recent outstanding reviews on them (Barclay, 2003; Brummendorf and Lemmon 2001; Panicker et al. 2003).

A controllability-based formulation for the topology optimization of smart structures

Juliano F. Gonçalves,Jun S.O. Fonseca,Otávio A.A. Silveira 국제구조공학회 2016 Smart Structures and Systems, An International Jou Vol.17 No.5

This work presents a methodology to distribute piezoelectric material for structural vibration active control. The objective is to design controlled structures with actuators which maximizes the system controllability. A topology optimization was formulated in order to distribute two material phases in the domain: a passive linear elastic material and an active linear piezoelectric material. The objective is the maximization of the smallest eigenvalue of the system controllability Gramian. Analytical sensitivities for the finite element model are derived for the objective functions and constraints. Results and comparisons with previous works are presented for the vibration control of a two-dimensional short beam.

Wear Resistant Duplex Stainless Steels Produced by Spray Forming

Juliano Soyama,Thiago Pama Lopes,Guilherme Zepon,Claudio Shyinti Kiminami,Walter José Botta,Claudemiro Bolfarini 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.2

In this work, boron-modified duplex stainless steels were prepared by spray forming using design guidelines provided bythermodynamic calculations. Firstly, an investigation of stable phases and phase formation sequence in duplex steels containinghigh levels of boron was conducted. The calculation indicated that there was an eutectic point at around 1 wt% boron withdifferent primary phase formations upon equilibrium solidification. For hypoeutectic compositions, the primary phase wasδ-Ferrite, whereas for hypereutectic a metallic boride (M2B) should form. Additionally, eutectic reactions for both compositionsshould lead to the formation of borides M2Band M3B2. Secondly, spray forming experiments were conducted basedon the thermodynamic calculations. Sample preparation was carried out using a conventional superduplex steel (2507) asstarting material. Two different compositions were selected: one hypoeutectic (0.8 wt% B) and one hypereutectic (2.5 wt%B). The microstructural investigation revealed the formation of different types of borides embedded in an austenitic-ferriticmatrix. Finally, the wear resistance was evaluated with the dry sand/rubber wheel test and a significant improvement wasobserved for boron-containing steels in comparison with the same steel without boron. This improvement was attributed tothe presence of fine and well-distributed boride particles that protected the austenitic-ferritic matrix from material removal.

Percepção de consoantes oclusivas em posição inicial de palavra por estudantes de língua portuguesa na Coreia do Sul

( Juliano Paiva Junho ),( Karina G. S. De Oliveira ) 한국포르투갈-브라질학회 2020 포르투갈-브라질 연구 Vol.17 No.2

Este artigo apresenta uma análise inicial de dados coletados sobre a percepção dos sons da língua portuguesa por falantes nativos de coreano, estudantes em fase inicial de aprendizado na BUFS (Busan University of Foreign Studies ─ Universidade de Estudos Estrangeiros de Busan), Coreia do Sul. Discutimos alguns dos diversos sistemas de romanização do coreano e suas idiossincrasias. Seguimos comentando brevemente os sistemas fonológicos de ambas a línguas e comparando-os frente a frente, demonstrando os seus pontos de divergência que podem vir a criar problemas quando do ensino da língua portuguesa. Continuamos discutindo o experimento elaborado com palavras selecionadas a partir dessas divergências dos sistemas fonológicos das línguas, apresentamos a metodologia pela qual os dados foram coletados e as formas de processamento computacional usadas. Por fim, focamo-nos na análise das consoantes oclusivas em posição inicial de palavras, visto que apresentam grande dificuldade para os aprendizes. Apresentamos os resultados obtidos dos alunos que demonstram que a percepção dos fonemas consonantais surdos (/p, t, k/) e sonoros (/b, d, g/) da língua portuguesa não é algo simples que se possa ensinar sem esforço. Comentamos, brevemente, ao final do artigo, propostas de abordagem didática para os problemas levantados sobre o ensino de fonética/fonologia do português para coreanos. This article presents an initial analysis of data collected on the perception of sounds of the Portuguese language by native Korean speakers, students in the initial learning phase at BUFS (Busan University of Foreign Studies - Busan University of Foreign Studies), South Korea. We discuss some of the several systems of Korean romanization and its idiosyncrasies. We continue commenting briefly on the phonological systems of both languages and comparing them face to face, demonstrating their points of divergence that may create problems when teaching Portuguese. We then discuss the experiment developed with words selected from divergent points in each language’s phonological system and present the methodology by which the data were collected, as well as the forms of computational processing used. Finally, we focus on the analysis of the occlusive consonants in word initial position since they present great difficulty for Korean students. We present results that the perception of voiceless (/p, t, k/) and voiced (/b, d, g/) consonant phonemes in Portuguese is not something simple that can be taught without effort. At the end of the article, we briefly propose a didactic approach on the problems raised about teaching Portuguese phonetics/phonology to Koreans.

Comparison of RULA and Checklist OCRA Ergonomic Risk Methods for Civil Construction

Juliano Prado Stradioto,Ariel Orlei Michaloski,Antonio Augusto de Paula Xavie,Daniela Colombini 대한산업공학회 2020 Industrial Engineeering & Management Systems Vol.19 No.4

The external plastering has a high rate of work accidents and corresponds to a large part of work-related musculoskeletal disorders (WMSD) in construction. The objective of this research was to statistically correlate the RULA and OCRA Checklist ergonomic risk methods, used in external workstations on building facades. The methodological approach consisted of a quantitative and qualitative research, including literature review and field research, including 32 employees, with the project approved by the Institution’s Ethics and Research Committee. This research showed results that indicate the high physical requirements that the activity exerts on workers, a strong and significant statistical correlation between the methods, and that the OCRA checklist method is the best option among those for the ergonomic evaluation. The research showed limitations regarding the amount of ergonomic methods used as well as the difficult access to construction sites. The research leaves the possibility of future studies on the statistical comparison between other ergonomic methods, using a greater number of statistical tools, in addition to analyzing other activities that make up the execution of building work in civil construction.

Combined PET and Biopsy Evidence of Marrow Involvement Improves Prognostic Prediction in Diffuse Large B-Cell Lymphoma

Cerci, Juliano J.,Gyö,rke, Tamá,s,Fanti, Stefano,Paez, Diana,Meneghetti, José,Clá,udio,Redondo, Francisca,Celli, Monica,Auewarakul, Chirayu,Rangarajan, Venkatesh,Gujral, Sumeet,G Society of Nuclear Medicine 2014 The Journal of nuclear medicine Vol.55 No.10

<P>Bone marrow is an important extranodal site in diffuse large B-cell lymphoma (DLBCL), and marrow histology has been incorporated into the new National Comprehensive Cancer Network international prognostic index. Marrow involvement demonstrated histologically confers poor prognosis but is identified by staging PET in more cases. How information from staging PET and biopsy should be combined to optimize outcome prediction remains unclear. <B>Methods:</B> The International Atomic Energy Agency sponsored a prospective international cohort study to better define the use of PET in DLBCL. As a planned subsidiary analysis, we examined the interplay of marrow involvement identified by PET and biopsy on clinical outcomes. <B>Results:</B> Eight countries contributed 327 cases with a median follow-up of 35 mo. The 2-y outcomes of cases with no evidence of marrow involvement (<I>n</I> = 231) were 81% (95% confidence interval [CI], 76%–86%) for event-free survival (EFS) and 88% (83%–91%) for overall survival (OS); cases identified only on PET (<I>n</I> = 61), 81% (69%–89%) for EFS and 88% (77%–94%) for OS; cases indentified only on biopsy (<I>n</I> = 10), 80% (41%–95%) for EFS and 100% for OS; or cases identified by both PET and biopsy (<I>n</I> = 25), 45% (25%–64%) for EFS and 55% (32%–73%) for OS. The hazard ratios for PET-negative/biopsy-negative cases versus PET-positive/biopsy-positive cases were 2.67 (95% CI, 1.48–4.79) for EFS and 3.94 (1.93–8.06) for OS. <B>Conclusion:</B> This large study demonstrates that positive iliac crest biopsy histology only confers poor prognosis for patients who also have abnormal marrow <SUP>18</SUP>F-FDG uptake identified on the staging PET scan. Abnormal <SUP>18</SUP>F-FDG uptake in marrow, when iliac crest biopsy histology is normal, has no adverse effect on outcomes.</P>

Symposia : Cell Adhesion and Migration ; Integrin-mediated control of cell signaling

( Rudy Juliano ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Characterization of arazyme, an exocellular metalloprotease isolated from <i>Serratia proteamaculans</i> culture medium

Bersanetti, Patrí,cia A.,Park, Ho-Yong,Bae, Kyung Sook,Son, Kwang-Hee,Shin, Dong-Ha,Hirata, Izaura Y.,Juliano, Maria A.,Carmona, Adriana K.,Juliano, Luiz Elsevier 2005 Enzyme and microbial technology Vol.37 No.6

<P><B>Abstract</B></P><P>We investigated the biochemical properties of a 51.5kDa metalloprotease (arazyme, 3.4.24.40) secreted into the culture medium by <I>Aranicola proteolyticus</I>, a symbiotic bacterium of the spider <I>Nephila clavata</I>. The enzyme was purified to apparent homogeneity by ion exchange chromatography in a Resource Q column (FPLC system). The substrate specificity requirements of purified arazyme were examined using fluorescence resonance energy transfer (FRET) peptides derived from the lead sequence Abz-KLRFSKQ-EDDnp (Abz=<I>ortho</I>-aminobenzoic acid; EDDnp=ethylenediaminedinitrophenyl). Three series of peptides were assayed to map the S<SUB>2</SUB>, S<SUB>1</SUB> and <SUB><SUP>S′</SUP>1</SUB> subsites: Abz-KXRFSKQ-EDDnp, Abz-KLXFSKQ-EDDnp and Abz-KLRXSKQ-EDDnp (X are natural amino acids). The results indicated that S<SUB>1</SUB> subsite has a broad specificity, being Gly the preferred amino acid for this subsite followed by positively charged residues (Arg and His). The S<SUB>2</SUB> and <SUB><SUP>S′</SUP>1</SUB> subsites accommodated better hydrophobic residues with aliphatic or aromatic side chains (Leu, Phe). The pH effect on hydrolysis of Abz-KLFFSKQ-EDDnp indicated that optimal hydrolysis occurred at pH 8.0 or higher. The effect of NaCl on the arazyme activity depends on the substrate, but in general the activity was reduced with this salt. The temperature did not affect the enzyme from 10 to 45°C, after which activity decreased sharply. Arazyme presented high hydrolytic activity on substance P and peptides related to bradykinin. In addition, arazyme activity was resistant to the treatment by pepsin, trypsin and chymotrypsin. In conclusion, arazyme has a broad hydrolytic profile and works in very aggressive conditions, which justify its potential use in therapeutics and biotechnological applications.</P>

Electronic Government in Brazil - Measuring E-Gov Appropriation by Citizens and Enterprises

Alexandre Barbosa,Juliano Cappi Winston Oyadomari,Ingrid Winkler 한국경영정보학회 2011 한국경영정보학회 학술대회논문집 Vol.2011 No.06

Maximal isometric handgrip strength: comparison between weight categories and classificatory table for adult judo athletes

Emerson Franchini,Juliano Schwartz,Monica Yuri Takito 한국운동재활학회 2018 JER Vol.14 No.6