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Purification and characterization of a thermophilic chitinase produced by Aeromonas sp. DYU-Too7
Jui-Rze Too,Te Sheng Lien,Shwu-Tzy Wu,Shin-Tsung Yu 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.5
extracellular chitinase, produced by Aeromonas sp. DYU-Too7, was purified in the following proce-phacryl S-100HR. The resulting chitinase has a molecular mas of 36 kDa, an optimal reaction pH of 5.0, and an optimalreaction temperature of 70oC. It retains almost 10% activity in the pH range of 5.0-8.0. This chitinase has a high ther-mal tolerance and retained 90% of its activity at 50oC and 75% at 60oC. Enzyme activity was inhibited by Ba2+, Hg2+,Mg2+ and Ag+ cations, but was not substantially inhibited by the K+ cation nor the chelating agent EDTA. The Km andVmax, using colloidal chitin as a substrate, are 6.3 g/L and 18.69mol/min/mg-protein, respectively. The 36 kDa chitinaseof Aeromonas sp. DYU-To7 is an exo-type enzyme, because chitobiose was the main hydrolysate in hydrolysis ofcolloidal chitin.
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Shwu-Tzy Wu,Yi-Chun Lin,Jui-Rze Too 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.2
Ralstonia eutropha was cultivated in a continuous stirred fermenter with various C/N ratios (20, 30, and 40), dilution rates, and organic salt substrates (sodium propionate or sodium valerate) to explore the microbial growth and the poly(3HB-co-3HV) accumulation. When sodium propionate was used as the secondary carbon source, the HB/HV molar ratio at various C/N ratios and dilution rates did not change appreciably (approximately 90 : 10). The highest poly(3HB-co-3HV) content in biomass (41.8%) and poly(3HB-co-3HV) productivity (0.100 g/(L·h)) occurred under the condition with a C/N ratio of 20 and dilution rate of 0.06 h−1. When sodium valerate was used as the secondary carbon source, the productivity of poly(3HB-co-3HV) increased with increasing dilution rate for the C/N ratio of 30 and 40. The average HB/HV molar ratio ranged from 48 : 52 to 78 : 32. The feeding of sodium valerate promoted the accumulation of HV better than feeding sodium propionate did. This study shows that a potential strategy of manipulating by both C/N ratio and dilution rate could be used to control the HV unit fraction in poly(3HB-co-3HV) in a continuous cultivation.
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