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Localization of dynamin-related protein 1 and its potential role in lamellipodia formation
Jo, Y.,Cho, H. M.,Sun, W.,Ryu, J. R. Springer Science + Business Media 2017 Histochemistry and cell biology Vol.148 No.1
<P>Dynamin-related protein1 (Drp1) plays an essential role in mitochondrial fission: Cytosolic Drp1 is translocated to the mitochondria upon stimulus, and oligomerized Drp1 constricts mitochondria by aid of actin filaments. Drp1 completes the fission process with GTP hydrolysis by its own GTPase activity. The importance of actin filament and its interaction with Drp1 in the mitochondrial fission process have been demonstrated. In this study, we found that Drp1 is enriched in the actin-rich leading edge of lamellipodia of mouse embryonic fibroblasts (MEFs) wherein mitochondria or peroxisomes are absent. Mff-binding mutant (A395D) of Drp1, which cannot be recruited to mitochondria, was also localized in lamellipodia, indicating that Drp1 in lamellipodia is not related to mitochondria. When lamellipodia formation was induced by platelet-derived growth factor (PDGF) in MEFs, S616 phosphorylated form of Drp1 was accumulated to the lamellipodia. Inhibition of Drp1 with Mdivi-1 or a specific shRNA significantly decreased PDGF-induced lamellipodia formation or initial cell spreading during re-plating of the cells, respectively. Interestingly, defective lamellipodia formation and cell adhesion caused by Drp1 inhibition were not rescued by supplementing L-carnitine, although it restored mitochondrial energy loss caused by Drp1 inhibition. Collectively, these results favor the idea that Drp1 might play a significant role in lamellipodia formation and cell spreading through a different mechanism from that used for regulating mitochondrial dynamics/function.</P>
Jo, Y.,Sanyal, B.,Park, H.J.,Kwon, J.H. Elsevier 2016 Postharvest biology and technology Vol.112 No.-
<P>The detection of cellulose radicals by ESR spectroscopy in oranges irradiated by different radiation sources has been studied during storage. The peel of oranges was found to be more suitable for ESR analysis in comparison with flesh parts because of its higher fiber and lower moisture contents. The oranges were exposed to gamma ray, electron-beam, and X-ray sources, and the paramagnetic centres were investigated. The samples extracted with absolute ethanol followed by oven drying showed a reduced drying time (five-fold) and enhanced signal sensitivity (double) in comparison to freeze dried samples. Further, the ESR spectrum showed that a radiation-induced signal could be detected in samples exposed to a dose above 0.4 kGy regardless of the radiation source. Irradiated oranges were stored at different conditions such as dark at 4 degrees C, fluorescent light at 20 degrees C, and indirect natural light, to study ESR spectral behavior. Fading of signal intensity was predominant when the samples were stored under natural and artificial light. The identification of cellulose radicals using ESR technique was improved for irradiated oranges containing a total fiber content of more than 60% (dry basis) after ethanol extraction even after a prolonged storage. (C) 2015 Elsevier B.V. All rights reserved.</P>
Jo, Y.,Kim, J.,Lee, C. Elsevier BV ; C/o Dept. of Chemical Engineering, N 2016 JOURNAL- TAIWAN INSTITUTE OF CHEMICAL ENGINEERS Vol.68 No.-
A downflow anaerobic filter packed with blast-furnace slag (BFS) grains was evaluated for treating cheese whey, a high-strength dairy effluent. The reactor demonstrated effective and stable biomethanation of whey in continuous mode without pH control. The chemical oxygen demand (COD) removal remained at around 80%, with the reactor pH being maintained neutral without buffering, in the organic loading rate (OLR) range of 0.8-2.4g COD/l@?d. Although the COD removal decreased down to below 60% at higher OLRs along with acid accumulation, the methane production rate continued to increase with increasing OLR. On the other hand, the methane yield was maintained at 0.28-0.34l/g COD throughout the experiment. The substrate removal and methane production kinetics were successfully described by the modified Stover-Kincannon model. The overall results suggest that BFS grains could be used as economic filter media for anaerobic treatment of cheese whey and other easily acidifying waste streams.