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국내에서 유행하는 HIV의 전파 경로에 따른 Subtype 분포
이주실,남정구,김성순,강춘,최병선,김옥진,박미선,성봉모,서순덕,전수경,변승옥,신영오,조해월 대한감염학회 2001 감염 Vol.33 No.5
Background : Previous data have been reported that subtype B is prevalent in South Korea, but neither the extent nor the proportion of subtypes could be evaluated. This study was designed to analyze the distribution of HIV-1 subtypes, temporal instructions and transmission dynamics between epidemiological groups. Methods : 1,280 Koreans had been diagnosed as HIV seropositive during the period 1985 to 2000. Among them, 134 individuals were selected for this molecular epidemiological study. 134 DNAs were isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 env gene was amplified by nested polymerase chain reaction and was sequenced. Results : HIV-1 isolates from thirty-seven homosexuals were all subtype B (100%). On the other hand, 66 isolates from 94 heterosexuals were subtype B (70%) and 28 were non B subtypes (30% : 13 A, 4 C, 2 D, 8 E , 1 G). Only subtype B strains were isolated from 73 males who were infected with HIV inside Korea while 16 B and 20 non B subtype strains were isolated from 36 males who were HIV infected outside of Korea. However, B and non B strains were isolated half and half from females who were infected inside Korea except one. Conclusion : The HIV-1 subtype B strains are prevalent in Korea from the early HIV infection until present in both homo and heterosexuals. Non B strains have been transmitted from men who were infected outside Korea to their spouses and casual partners. So, we need further study to monitor subtype B and non B HIV transmission in epidemiological groups of Korea, (Korean J Infect Dis 33:311∼318, 2001)
황해 동남 해역의 수괴지표성 요각류 및 모악류의 분포와 수괴특성
박주석,이삼석,강영실,이병돈,허성회 동의대학교 기초과학연구소 1993 基礎科學硏究論文集 Vol.3 No.1
Distribution of indicator species of copepods and chaetognaths were studied as an indicator species of water mass in the southeastern area of the Yellow Sea. Undinula darwini, Lucicutia flavicornis, Pleuromamma gracilis, Euchaeta resselli, Euchaeta plana and Sagitta enflata were found to be reliable indicator species for determining warm water mass. Of these species, E. plana and E. russelli have a weak tolerance on the low temperature. Sagitta crassa was indicator species of neritic waters: Sagitta bedoti was that of mixing waters. Centropages agdominalis represented neritic cold waters. In February U. darwini, L. flavicornis, P. gracilis, E. russelli, E. plana and S. enflata occurred in the western waters of Cheju-Do where warm waters over 14℃ occupied. Centropages abdominalis occurred in the northern area beyond Chindo with water temperature less than 10℃. E. plana, E. russelli and S. bedoti were found at the regions between Cheju-Do and Chindo where the water temperature was 12∼14℃ corresponding to the mixing waters. Based on cluster analysis and T-S diagram in February three different water masses were identified from the south to the north. In August, water masses were analyzed at two different layers, 0∼20m and 20m∼bottom layers, separated by thermocline depth. In 0∼20m layer, E plana and E. russelli were found from the western waters of Cheju-Do to Daehuksando. In 20m∼bottom layer, E. russelli and E. plana occurred at the northwestern waters of Cheju-Do with the water temperature warmer than 12℃. C. abdominalis was found at the northern area beyond Chindo. Based on the cluster analysis and T-S diagram in August three different water masses at 0∼20m and 20m∼bottom layers were identified from the coast to the offshore. C. abdominalis was found at the adjacent water of Chindo at 0∼20m layer and the northern area beyond Chindo at 20m∼bottom layer. This fact suggested that the cold water mass existed at the adjacent waters of Chindo in summer.
Inhibition of Osteoclast formation from cultured synovial cells by Dexamethasone
( Yeong Shil Joo ),( Myeong A. Cheong ),( Jae Il Park ),( Min Ju Kang ),( Byoung Joon Kim ),( Kyu Jeung Ahn ),( Sung Woon Kim ),( Jin Woo Kim ),( Yeong Seol Kim ),( Young Kil Choi ) 대한내과학회 2001 대한내과학회 추계학술대회 Vol.61 No.-
Rapid Dissemination of Newly Introduced Plasmodium vivax Genotypes in South Korea
Kim, Yeon-Joo,Kim, Jung-Yeon,Lee, Eun-Gyu,Lee, Byeong-Chul,Cho, Shin-Hyung,Yu, Jae-Ran,Rhie, Ho-Gun,Choi, Yien-Kyoung,Lee, Ho-Sa,Lee, Joo-Shil,Kim, Tong-Soo,Choi, Kyung-Mi,Park, Mi-Hyun American Society of Tropical Medicine and Hygiene 2010 The American journal of tropical medicine and hygi Vol.82 No.3
<P>Reemerged Plasmodium vivax malaria in South Korea has not yet been eradicated despite continuous governmental efforts. It has rather become an endemic disease. Our study aimed to determine the genetic diversity in P. vivax merozoite surface protein-1 (PvMSP-1) and circumsporozoite protein (PvCSP) genes over an extended period after its reemergence to its current status. Sequence analysis of PvMSP-1 gene sequences from the 632 P. vivax isolates during 1996-2007 indicates that most isolates recently obtained were different from isolates obtained in the initial reemergence period. There was initially only one subtype (recombinant) present but its subtypes have varied since 2000; six MSP-1 subtypes were recently found. A similar variation was observed by CSP gene analysis; a new CSP subtype was found. Understanding genetic variation patterns of the parasite may help to analyze trends and assess extent of endemic malaria in South Korea.</P>
주영실 ( Yeong Shil Joo ),이신석 ( Shin Seok Lee ),김완욱 ( Wan Uk Kim ),민준기 ( Jun Ki Min ),박성환 ( Sung Hwan Park ),홍연식 ( Yeon Sik Hong ),이상헌 ( Sang Heon Lee ),조철수 ( Chul Soo Cho ),김호연 ( Ho Youn Kim ) 대한류마티스학회 2000 대한류마티스학회지 Vol.7 No.1
Objectives: To assess the extent of generalized osteoporosis in Korean rheumatoid arthritis patients and evaluate the importance of disease activity, duration of disease, menopausal status, corticosteroid use and markers of bone metabolism. Methods: Bone mineral density was measured by dual energy x-ray absorptiometry (DEXA) at 3 locations in 134 rheumatoid arthritis patients, aged 21~80 (57 premenopausal and 66 postmenopausal patients). Markers of bone metabolism were measured and assessed in relation to the disease activity and corticosteroid use. Results: The mean age of the study population was 49 years and mean age of the menopause was 48.1±3.6 years. Decreased bone mineral density was observed at all 3 locations measured in the study population (Z-score -0.36, -0.14 and -0.66 for BMD of L-spine, femoral neck and femur Ward`s triangle respectively). Fifty nine percent of the patients showed osteopenia (T-score <-1) and 13.4% showed Tscore lower than -2.5. The best independent predictors of bone mass (stepweise multiple regression analysis) was body mass index, cortocosteroid use, and CRP in premenopausal patients and years post menopause, age, alkaline phosphatase and rheumatoid factor in postmenopausal patients. Urinary excretion of deoxypyridinoline was increased in both pre- and postmonopausal RA patients while serum osteocalcin level was normal in both groups. Deoxypyridinoline level was significantly correlated with CRP in premenopausal RA patients. Conclusion: Generalized osteoporosis is also prevalent in Korean rheumatoid arthritis patients. Bone metabolism appears to be uncoupled. Deoxypyridinoline correlated best with CRP and thus can provide a rational approach for selecting and treating patients with RA to reduce the risk of osteoporotic fracture.
류마티스관절 활막세포에서의 Receptor Activator of NF-kB Ling (RANKL)의 발현 및 파골세포 생성
주영실 ( Yeong Shil Joo ),정명아 ( Myeong A Cheong ),신동혁 ( Dong Hyuk Sheen ),임미경 ( Mi Kyoung Lim ),심승철 ( Seung Cheol Shim ),정덕환 ( Duke Whan Chung ) 대한류마티스학회 2003 대한류마티스학회지 Vol.10 No.4
Objective: A number of soluble factors which play important role in the pathophysiology of rheumatoid synovitis are also known to be involved in osteoclast differentiation and activation through RANKL (Receptor activator of NF-κB ligand). To investigate the importance of RANKL in the pathogenesis of bone erosion in rheumatoid arthritis (RA) patients, we analyzed the expression of RANKL and Osteoprotegerin (OPG) and examined the formation of osteoclasts in rheumatoid synovial fibroblasts under the influence of various osteotropic factors. Methods: Primary culture synoviocytes or fibroblast-like synoviocytes isolated from synovial tissues of 8 RA patients were cultured and treated with IL-1β (2 ng/ml), TNF-α(2 ng/ml), INF-γ (1000 /ml), IL-15 (10 ng/ml), IL-12 (10 ng/ml), dexamethasone (10(-9) M), PMA (10 ng/ml) or 1,25 (OH)2D3 (10(-9) M) for 18 hours. Expression RANKL or OPG mRNA was measured by semiquantitative RT-PCR within linear amplification condition. TRAP (+) MNC (tartrate resistant acid phosphatase-positive multinucleated cell) formation was induced from primary culture synoviocytes or in coculture system of synovial fibroblasts with PBMCs in the presence of M-CSF and 1,25 (OH)2D3. Results: 1. The intensity of base-line expression was different from patient to patient. Primary culture synoviocytes and synovial fibroblasts express RANKL and OPG mRNA with decreasing intensity when they are passaged. 2. Expresssion of RANKL mRNA was significantly increased by 1,25 (OH)2D3 and IL-1β (158.8±21% and 197.2±17% of controls, p<0.05 and p<0.005, respectively), while decreased significantly by dexamethasone (25.6±4.6% of controls, p<0.005). Expression of RANKL mRNA was significantly increased by IL-1β and decreased by dexamethasone, in a dose- and time-dependant manner. 3. TRAP (+) MNCs are formed from primary culture synoviocytes or in coculture system of synovial fibroblasts and PBMC in the presence of M-CSF and 1,25 (OH)2D3. Dexamethasone clearly inhibited TRAP (+) MNCs formation from synovial cells. Conclusion: The regulatory mechanism for the expression of RANKL or OPG in rheumatoid synoviocytes might be different from that in bone marrow cells. Modulating the expression of these molecules could have potential therapeutic implication targeting bone destruction in RA.