KI-180과 KI-188 칼슘조성물이 어린쥐의 성장발육에 미치는 영향

박승만(Seung-Man Park),성기승(Ki-Seung Seong),이종석(Jong-Seok Lee),이옥환(Ok-Hwan Lee),한찬규(Chan-Kyu Han) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.3

본 연구에서는 KI-188 칼슘과 KI-180 조성물이 성장발육에 미치는 효과를 조사하였다. 시험기간중 KI-188 칼슘과 KI-180 식이군이 대조군 보다 성장률과 평균 식이섭취량은 증가하였다. 체장은 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 평균 3.55 mm 더 길었고, 등뼈길이는 KI-180 식이군이 대조군 보다 통계적인 차이는 없었지만 0.5 mm 더 길었다. 대퇴골 무게는 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 통계적으로 무거웠으며, 대퇴골길이는 대조군에 비해 각각 평균 0.89, 1.09 mm 더 길었다. 혈당, 총콜레스테롤 및 중성지방 농도는 차이가 없었고, 칼슘농도는 KI-188 칼슘과 KI-180 식이군 보다 대조군이 유의하게 높았다. 백혈구와 혈소판수는 차이가 없었고, 적혈구, 혈색소 및 헤마토크릿치는 대조군이 KI-188 칼슘과 KI-180 식이군 보다 통계적으로 높았다. ALP활성은 KI-180 식이군이 대조군 보다 통계적으로 높았고, osteocalcin농도는 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 통계적으로 유의하게 높았다. 혈청 testosterone 농도는 KI-188 칼슘과 KI-180 식이군과 대조군 간에 차이가 없었다. IGF-1과 IGFBP-3 농도는 KI-180 조성물이 대조군 보다 각각 20%, 11% 정도 유의하게 더 높았다. 이상의 결과를 검토할 때 KI-188 칼슘과 KI-180 조성물은 어린쥐의 성장과 골격의 발달을 촉진시키고, 성장호르몬의 분비능을 양적으로 유도하여 전반적인 성장발육에 유효한 작용이 있는 것으로 사료된다. Herbs have active components that promote the growth rate of both animals and human. The KI-180 and KI-188 calcium food formulae contain Acanthopanacis cortex, Bombysis corpus and hoelen, seaweed calcium, chlorella extract, spirulina, colostrum powder, and other natural and functional components. We evaluated the growth-promoting effects of these formulae by analyzing the weight, femur and backbone, alkaline phosphatase, osteocalcin, testosterone, insulin-like growth factor (IGF)-1, and IGF binding protein-3 (IGFBP-3) of growing rats. Growing rats administered with KI-180 and KI-188 calcium showed the increase of body weight, body length, and femur weight and length of growing rats. In addition, administration of KI-180 and KI-188 calcium increased the alkaline phosphatase activity, the levels of osteocalcin and the growth hormones IGF-1 and IGFBP-3 of growing rats. The impact of KI-180 and KI-188 calcium on the physical development of growing rats suggests that the incorporation of these food formulae in the diets of growing children may promote the physical development.

광선각화증, 보웬병, 편평상피세포암에서 Ki-67, Cyclin A, p53, p16의 발현 양상

이효진 ( Hyo Jin Lee ),신동훈 ( Dong Hoon Shin ),최종수 ( Jong Soo Choi ),김기홍 ( Ki Hong Kim ) 대한피부과학회 2012 대한피부과학회지 Vol.50 No.4

Background: Actinic keratosis (AK) and bowen`s disease (BD) are pre-cancerous diseases, and are regarded as an early squamous cell carcinoma (SCC). AK and BD can be progressed into SCC. In this process, tumor suppressor and cell proliferative proteins may play important roles. Objective: To investigate the differences of expression patterns of the immunohistochemical (IHC) staining and useful markers for differential diagnosis in AK, BD and SCC. Methods: Biopsy had proven 17 cases of AK, 20 cases of BD and 17 cases of SCC, which were all selected. IHC staining for Ki-67 and cyclin-A, as cell proliferative markers, p53 and p16 as tumor suppressor markers, were performed. Labeling index (LI) and distribution pattern of IHC expressions were measured. Results: LI of Ki-67 in AK, BD and SCC were 30.6%, 60.2% and 54.8%, respectively. LI of cyclin-A in AK, BD and SCC were 9.2%, 24.4% and 24.1%, respectively. LI of p53 in AK, BD and SCC were 20.7%, 37.9%, and 39.9%, respectively. LI of p16 in AK, BD and SCC were 10.6%, 38.3% and 39.9%, respectively. Lower 1/3 was the most frequent distribution pattern in AK in all IHC stains, full thickness lower 2/3 were the most frequent distribution pattern in BD and SCC in all IHC stains. Conclusion: LI and distribution pattern of Ki-67, cyclin-A, and p16, as well as the distribution pattern of p53 may be useful markers to differentiate AK from BD and SCC. Higher degree and full-thickness distribution pattern IHC expressions in all stains may be helpful in the diagnosis of BD, rather than AK. (Korean J Dermatol 2012;50(4):290∼298)

뇌동맥류 수술시 체성감각유발전위의 모니터링

염기훈,김국기,이기택,박종태,임영진,김태성,이봉암,임언 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.4

Ki-67 Expression Gives Additional Prognostic Information on St. Gallen 2007 and Adjuvant! Online Risk Categories in Early Breast Cancer

Jung, So-Youn,Han, Wonshik,Lee, Jong Won,Ko, Eunyoung,Kim, Eunkyu,Yu, Jong-Han,Moon, Hyeong-Gon,Park, In Ae,Oh, Do-Youn,Im, Seock-Ah,Kim, Tae-You,Hwang, Ki-Tae,Kim, Sung-Won,Noh, Dong-Young Springer - Society of Surgical Oncology 2009 Annals of Surgical Oncology Vol.16 No.5

Hypoxia Enhances Nitric Oxide Synthesis by Upregulation of Inducible Nitric Oxide Synthase in Endothelial Cells

Ki?Jong Rhee,Sun-Yeong Gwon,and Seunghyung Lee 대한의생명과학회 2013 Biomedical Science Letters Vol.19 No.3

Hypoxia is an integral part of the environment during luteolysis. In this study we examined whether hypoxia could directly stimulate endothelial cells to produce nitric oxide (NO). Endothelial cells were cultured in hypoxic (5% O₂) or normoxic (20% O₂) conditions and the levels of total NO, inducible NO and endothelial NO was measured. We found that hypoxia but not normoxia upregulated NO production. The increased NO levels correlated with increased inducible NO synthase (iNOS) expression whereas expression of endothelial NOS (eNOS) expression remained constant. Addition of the iNOS specific inhibitor 1400W to hypoxic cultures prevented NO production suggesting that hypoxia?induced NO production in endothelial cells was due mainly to upregulation of iNOS. We also found that prostaglandin F2α (PGF) production was unaffected by hypoxia suggesting that upregulation of NO was not due to increased synthesis of PGF. In summary, we report that endothelial cells cultured under hypoxic conditions produce NO via the iNOS pathway. This study provides the importance of the relation between the hypoxic environment and the induction of NO by endothelial cells during regression of the corpus luteum in the ovary.

트랜시스 싱글 존 모델을 이용한 멀티 존 에너지 해석 방법

전병기(Jeon, Byung?Ki),김의종(Kim, Eui-Jong) 한국건축친환경설비학회 2016 한국건축친환경설비학회 논문집 Vol.10 No.5

The purpose of this work is to propose a simple single-zone model for analyzing multi-zone cases. Multi-zone models can be defined directly describing the building plan while important simulation data and careful parameterization are required. In contrast, the single-zone model is set in a very simplified manner, so many engineering projects prefer such a model. However, differences between the two models are not yet well investigated, particularly in a quantitative way, and thus the paper tests the differences and proposes possible methodologies to enhance the single-zone model to be closer to the multi-zone model. Thus, the balcony is modeled in detail to account for the solar double transmitted irradiance via windows at both sides of the balcony. Since the solar transmitted irradiance directly influences building loads, a special attention is required. In addition, the internal walls are taken into account in the single-zone model to keep the similar thermal capacity of multi-zone cases. Results show that the proposed single-zone model predicts very similar annual loads with the reference multi-zone model (about 5% error) while the initial single-zone model without the methodologies is far from the reference model (more than 20% error).

아산시 신정호소의 수질오염 현황 및 부영양화 저감 방안

박종안,이종화,장봉기 순천향대학교 기초과학연구소 1998 순천향자연과학연구 논문집 Vol.4 No.1

This study was conducted to estimate the amount of water pollution load, water quantity and quality of inflow stream, status of water pollution and reduction of eutrophication in the Sinjung lake in Asan city. Inflow of branch to Sinjung lake have two streams, one is Cho-sa stream which are Ki-san bridge and Cho-sa bridge and the other is Hwang-san stream. Number of sampling points for water quality are 23 in the Sinjung lake and it's sediment of bottom from March to July, 1997. Water quality had been tested pH, dissolved oxygen(DO), chemical oxygen demand(COD), suspended solid(SS), total nitrogen(T-N), total phosphorus(T-P), E.coli group, and spring out test for sediment are total nitrogen, total phosphorus and heavy metals(Fe, Pb, Cd). The results were as follows; Amounts of pollution load were highest at Cho-sa stream. The ratio of livestock wastewater was occupied with the above 90% of total water pollution load. Mean daily inflow of Cho-sa stream and Hwang-san stream were 34,092㎥ and 25,524㎥, respectively. In mean level of inflow water quality, COD, SS, T-N, T-P and E. coli group were 16.96㎎/ℓ, 50.3㎎/ℓ, 5.85㎎/ℓ, 0.51㎎/ℓ and 4,096/100㎖, respectively. Water quality of inflow was worst at the point of Ki-san bridge in Cho-sa stream. Mean level of DO, COD, SS, T-N, T-P and E. coli group were 6.43㎎/ℓ, 17.11㎎/ℓ, 194.0㎎/ℓ, 5.96㎎/ℓ, 0.56㎎/ℓ and 2,191/100㎖, respectively at Sinjung lake. These are higher than class V by environmental water quality standards for lake. Spring out level of T-N, T-P, Fe and Pb in sediment of Sinjung lake were 326.6ppm, 17.9ppm, 624.2ppm and 2.65ppm, respectively.

카드뮴의 중추신경계 독성유발 기전

이종화,장봉기,박종안,박종영,김완종,우기민 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.3

Although numerous studies have shown that cadmium disturbs the normal biological processes in central nervous system the mechanism of toxicity is not well understood The present study has investigated the effect of cadmium on oxidative stress, Na+/K- ATPase activity and the aggregation of amyloid beta peptide(β-amylotd) in neuronal cell Ime, HT22 cell LC_(5) and LC_(50) of cadmium for HT22 cell resulted from MTT assay was 4 1 μM and 9 5 μM, respectively Cadmium(2 to 8 μM) dose-dependently increased the lipid peroxidation and decreased the content of glutathione Cadmium 4 μM showed a significant decrease in Na^(-)/K^(+) ATPase activity as compared with control group The aggregation of P-amyloid was accelerated in a dose-dependent manner by the treatment with 2 to 8 μM cadmium These results suggest that the neurotoxicity of cadmium can be mediated by the increase in oxidative stress and decrease in Na^(-)/K^(+) ATPase activity.

개에서 발생한 수컷 가성 반음양 1례

이기찬,손정민,윤기영,최호정,박성준,이영원,정성목,신상태,조종기 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2006 動物醫科學硏究誌 Vol.14 No.-

In this report, we present a case of the male pseudohermaphrodite in a beagle dog. The animal was subjected for examination due to enlargement of clitoris that was shown as reddish finger-like structure protruding from the vulva. Visual examination of the internal genitalia revealed that the animal possessed both testes and uterus. Based on the findings of physical examination and exploratory laparotomy, it was tentatively diagnosed as male pseudohermaphrodite. Internal genitalia and enlarged clitoris were removed for the prevention of pyometra and Sertoli cell tumor.

韓國産 夜行性 맵시벌科(벌目: 벌亞目)의 分類學的 硏究

이종욱,정종철,김기범,김정한,배지득,남상호 대전대학교 기초과학연구소 2002 自然科學 Vol.13 No.2

영남대 생물학과에 소장되어 있는 표본과 2001년 4월~2002년 6월 사이에 채집한 표본을 대상으로 하여 한국산 야행성 맵시벌의 분류학적 연구와 출현 현황 및 숙주 관계의 분석을 실시하였다. 분류학적 연구 결과 Gauld(1970)가 영국산 야행성 맵시벌을 대상으로 한 연구에서 기록한 Mesochorinae아과, Tryphonin-ae아과, Ctenoplematinae아과, Ophioninae아과의 4아과외에 Metopinae (Metopius속)를 추가하여 한국산 야행성 맵시벌류는 총 5아과 13속을 기록하였다. 또한 한국산 야행성 맵시벌의 출현 현황을 분석한 결과 야행성으로 잘 알려진 Mesochorinae아과의 Mesochorus속, Tryphoninae아과의 Netelia속과 Ophioninae아과가 전체 야행성 맵시벌 증 95%를 차지하였다. 속별 출현현황을 살펴보면 Netelia속이 393개체가 출현하여 가장 많은 수를 나타내었으며 Ophi-on속, Mesochorus속, Enicopilus속도 전체 출현 분류군 중 높은 비중을 차지하였다. 월별 현황을 살펴보면 전반적으로 4월에서 11월 사이에 걸쳐 나타나는데, Op-hioninae아과는 5월과 7월에, Tryphoninae아과는 6월에 집중적인 출현 빈도를 보였다. 숙주의 분류군에 따른 야행성 맵시벌의 기생 양상을 살펴보면 대부분이 나비목을 대상 숙주로 하고 있으며 Mesochorinae아과와 Ctenopelmatinae아과는 벌목에도 많은 종이 기생하는 것으로 나타났다. 특히 Mesochorinae아과의 Cidaphus속은 위생 해충으로 분류되는 기생파리과(Tachinidae)에도 기생하는 것으로 나타나 위생 해충을 구제할 수 있는 천적 분류군으로 연구될 수 있는 가능성을 보였다. 숙주의 식성에 따라 살펴보면 부식성의 먹이를 섭식하는 부식성(saprophagus) 숙주, 곤충의 유충을 섭식하는 식충성(entomophagus)인 숙주가 소수 출현하였고 기생생활(parasite)을 하는 숙주도 다수 출현하였으나 대부분의 야행성 맵시벌의 기생 대상 숙주는 주로 식물을 해하는 식식성 곤충(phytophagus)으로 나타났다. Nocturnal Ichneumonidae, which was called yellow-brown ichneumonoid, included several subfamilies adapted for nocturnal activity. Taxonomic study of nocturnal ichneumonoid has been investigated by Gauld(1975) but examination of Korean nocturnal ichneumonoid has not been provided until now. This present study: the taxonomic revision, Appearance state and Host-parasite relation analysis of nocturnal ichneumonoid, were examined using Korean peninsula specimens. As the result. approximately 13 genera referable to 5 subfamilies appeared regularly in light trap. Genus MetopiudSubfamily Illetopinae) were newly recorded in nocturnal ichneumonoid. Tryphoninae and Ophioninae, which were dominant in appearance taxa! occupied 96% of total numbers of individuals, and majority of them were taken from May to November. Nocturnal ichneumonoid are parasitoids of part of immature holometabolous insects(Coleoptera, Diptera, Hymenoptera, Lepidoptera). Lepidoptera is the most common hosts of nocturnal group except Mesochorinae and Ctenopelmatine. In large number of Mesochorinae are koinobiont hyperparasitoids of ectoparasitic or endoparasitic BraconiddHymenoptera), and, less frequently, of Tachinidae(Diptera1. Also, Ctenopelmatinae are koinobiont endoparasitoids of Symphyta and, rarely, Lepidoptera. Majority of nocturnal ichneumonoid hosts are phytophagous, feeding plant taxa, and many host of Mesochorinae taxa are parastoids, and a few host are saprophagus or entomophagus.