Confirmation of F1 Hybridity Using RAPD Markers in Soybean

Jong-II Chung,Mi-Suk Ko,Jung-Hyun Shim,Seok-Hyeon Kim,Jin-Ho Kang 한국자원식물학회 1999 Plant Resources Vol.2 No.1

Molecular markers are useful to confirm the hybridity of F1 plant derived from cross of two homozygous parents with similar morphological traits. RAPD markers were used to test F1 hybrid plant obtained from cross of two homozygous soybean (Glycine mar) parents. F1 plant for cross I was made from the mating of Hobbit87 (female) and L63-1889 (male) and F1 plant for cross II was obtained from the mating of H1053 (female) and L63-1889 (male). Selfing plant per each cross was also obtained. Among 20 Operon primers used, OPA04 and OPA09 show polymorphism between cross I and II parent. Band in size 1Kb of OPA04 and 2.1Kb of OPA09 primer was polymorphic band. This fragment identified F1 hybrid plant and selfing plant in cross I and II. Female parent Hobbit87 in cross I and H1053 in cross II has no this fragment (recessive allele). However, male parent L63-1889 and F1 hybrid plant in cross I and II has this size of polymorphic band (dominant allele). This indicated that F1 hybrid and selfing plants were detected by RAPD marker before phenotypic marker would be used to identify F1 hybridity. Amplification products of selfing plant for cross I and II were completely same to the those of female parent. When mature, flower color of F1 hybrid plant in cross I and II was purple and flower color of selfing plant in cross I and II was white. Purple flower is dominant trait. F1 hybridity was successfully detected at very early growth stage using RAPD marker. Therefore, RAPD marker can be used broadly to confirm F1 hybridity in many crops.

구후차단을 이용한 백내장수술시 진정 목적으로 사용한 Midazolam 과 Midazolam-Ketamine 의 비교

김진수,박종완,김일호,한찬수,김천숙,김유재,안기량 대한마취과학회 1998 Korean Journal of Anesthesiology Vol.34 No.6

Background : Many ophthalmic procedures can be performed using a retrobulbar regional anesthetic technique. However, retrobulbar block is painful and most of patients express anxiety about the procedure. In addition, several life-threatening complications may occur. We compared the effects of midazolam and midazolam-ketamine as a sedative during retrobulbar block in cataract surgery. Methods : Thirty patients undergoing cataract surgery were randomly allocated into two groups, group I(n=15) was received midazolam and group II(n=15), midazolam-ketamine. Mean arterial pressure(MAP), heart rate(HR), and peripheral oxygen saturation(SpO2) were compared before administration of drugs and 1, 2, 3, 4, 5, 10, 20, and 30 min after administration of drugs. Patients' movement requiring restraint were also checked. In the recovery room, postoperative nausea and vomiting, recall, delirium and/or hallucinations, and ocular complications were recorded. Results : There were no significant differences in MAP and SpO2 between groups but heart rates were significantly increased at 1, 2, 3, 4, and 5 min than baseline in group II. Movement score was significantly lower in Group II than in Group I during the block(p<0.05). Recall during performance of the nerve block occured more often in Group I than in Group II(p<0.05). Conclusions : Low-dose midazolam-ketamine sedation sequence was superior to a midazolam technique regarding patients' movement and recall. (Korean J Anesthesiol 1998; 34: 1136∼1143)

數種의 글라스 아이오너머 修復材의 弗素 遊離量과 修復物 邊緣 琺瑯質에 吸收된 弗素 含量에 관한 硏究

김종수,손일수,김용기 단국대학교 치의학연구소 1992 논문집 Vol.4 No.-

In this study, various kinds of glass ionomer filling materials were evaluated for fluoride release and uptake by adjacent enamel. Fuji Ⅱ, Ketac-Fil, Ketac-Silver were used for this study and Silux plus was selected for control group. Measurements of fluoride concentration released in solution from discs made of selected materials were performed daily for the first 7 days and on the 14th, 21st day after the experiment. In order to evaluate the amount of fluoride uptake by enamel adjacent to restorations, pre-op and post-op enamel biopsy were done on every sample teeth. Ketac-Fil showed the highest amount of fluoride release among tested materials through whole experimental periods. Statisically significant differences between pre-op and post-op enamel fluoride concentration could be found in Ketac-Fil from the first week and in Fuji Ⅱ from the 2nd week after fillings.

시화호의 배수갑문 운용에 따른 수질변화

김종구,김준우,조은일 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

To evaluate the change for water quality after the water gate operation in Shiwha lake, in situ survey were conducted on september in 2000 and January, march, jane in 2001. Chemical characteristics and eutrophication level was estimated estimated from the survey data. The water quality of the Shinhwa lake was greatly affected by pollutant load from rainfall, and formation of stratification of chlorophyll-a was occurred in autumn, due to increased nutrient, high water temperature and low salinity after rainfall runoff. The mean concentration of DIN, DIP were 0.346mg/L 0.0217mg/L in surface water and 0.826mg/L, 0.0415mg/L in bottom water, respectively, which were over Ⅲ grade of seawater standard. Also high percentage of ammonia nitrogen to DIN in bottom water for autumn and winter was affected by released nutrient from sediment Correlation analysis of chlorophyll-a versus TSS was shown that organic matter was affected by autochthonous organic matter stem from the algae, these factor showed reverse correlation about salinity. Closely correlations among to the water quality constituent in continuity survey was appeared. The results of eutrophication index estimation showed the high potentiality of red tide occurrence in Shiwha lake, particularity in summer or fall. Overall water quality was greatly improve to compared with measuring data during 1997∼1998 at the beginning water gate operation, which reported by KORDI. Therefore, to improve of water quality in Shinwha lake, we need to establish of managemant plan about nutrient release from sediment, rainfall, maximum of seawater exchange.

목형의 엔드 밀링시 절삭조건에 따른 가공 특성에 관한 연구

김성일,김종택,김민호,신형곤,김태영 한국공작기계학회 2006 한국공작기계학회 추계학술대회논문집 Vol.2006 No.-

The cutting tests of wood patterns are carried out using CNC milling machine. The cutting forces and surface characteristics of machined surface are investigated at various cutting conditions such as spindle speed, feed speed, cutting direction of wood pattern and wood material. In the CNC end-milling, the surface roughness increases as feed speed increases and decreases as spindle speed increases. However, the cutting force and surface roughness are different along the cutting direction and material of wood pattern.

새마을 所得增大의 主要作物들에 대한 蛋白質, 油分含量 및 脂肪酸 分析

李洪宰,李鐘一 순천대학 새마을연구소 1986 새마을硏究論文集 Vol.2 No.-

Recently, researches on oil crops in Korea were breeding on edible oil crops such as rapeseed sesaeme, peanut, perilla. Numerous varieties were reieased as a result of ective breeding works on edible oil crops, that is 7 rape varieties including Yudal, Mokpo-11, Yongdang, Nozeogchae, Naechan, Yeongsanyuchae and Cheongpungyuchae(hybrid), 5 varieties sesame including Suweon-5, 9, 21, Kwangsanggae and Danbaekggae, 5 peanut varieties including Seoduntangkong, Yeonghotangkong, Oltankong, Shinpungtangking and Seadltang-kong, 3 perilla varieties including Daegu, Suweon 8 and 10, respectively. This varieties showed a good oil quality with high oleic and linoleic acids content, but perilla oil seemed to be unsuitable for a edible use, since about 53 percent of its fatty acids was in the form of unsaturated linolen-ic acid.

새마을 所得增大의 主要作物인 良質油·粕油菜品種의 量的形質에 대한 遺傳的 變異와 選拔에 관한 硏究

權炳善,李鐘一 순천대학 새마을연구소 1986 새마을硏究論文集 Vol.2 No.-

This study was conducted to establish gene source bank of heterosis breeding in rapeseed, improvement of double zero 919 lines in Mokpo branch station. From this population, the range of phenotypic variations and the amount of genetic variations for several important agronomic characters were estimated to aid on important of the characters. An attempt was also made to get available information on the relative importance of yield compo-nents for developing high yielding variety. The yield per plant was 46g and majority of lines were ranged between 20g and 92g. The seed setting rate ranged from 23% to 100% with the aver-age of 94% and the average of flower emergence was April 5. A higly significant positive correlation was found between yield and plant height, ear length, total branch, 1st branch, 2nd branch, number of pods per ear, respectively. The flower emergence, plant height, ear length, branch(total, 1st 2nd) number of pods per ear, number of seeds per pod, seed setting rate were revealed relatively high heritability. However, plant height, ear length and 1st branch showed high heritabi-lity estimate with low genetic advance which may be due to the narrow range of phenotypic variation.

흰쥐 치아 재식 후 치수 치유 양상의 조직학적 관찰

고은진,정한성,김의성,정일영,이승종 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.4

본 연구의 목적은 흰 쥐 (rat) 를 이용하여 미성숙 단계의 치아를 발치 후 즉시 재식 하였을 때 나타나는 치수의 치유 양상을 조직학적으로 관찰하고자 하는 것이다. 생후 4주된 암컷 Sprague-Dawley 계 흰쥐의 상악 우측 제1대 구치를 발거 후 원래의 치조골와 내로 위치시켰다. 재식 후 3일째부터 국소적으로 치수 내 염증 소견이 관찰되었으나, 치근 부위에서는 이미 치수의 재혈관화 및 치유가 진행되고 있는 소견이 관찰되었다. 재식 후 5일째 부터는 odontoblast-like cell이 관찰되기 시작하였다. 삼차 상아질의 형성은 재식 후 1주째 부터 관찰되기 시작하였으며, 2주째부터는 확실히 관찰할 수 있었다. Odontoblast-like cell 및 삼차상아질 형성은 4주째 까지도 계속 관찰되었다. 재식 후 4주째에는 bone-like tissue 및 cementum-like tissue이 형성되었음을 관찰하였다. 본 실험을 통하여 흰 쥐 치아 재식시 석회화 과정은 초기에는 삼차상아질 침착에 의해서 진행되나, 시간이 경과하면서 점차 bone-like tissue 또는 cementum-like tissue가 차지하는 비율이 증가하는 것을 관찰하였다. The objective of this study was to observe the histology of dental pulp healing after tooth replantation in rats. The maxillary right first molars of 4-week-old rat were extracted. and then the teeth were repositioned in the original socket. At 3 days after replantation. there was localized inflammatory reaction. But, pulp revasculization and healing had already begun in the root area. At 5 days after replantation. odonto blast-like cells were observed. Tertiary dentin deposition was observed beneath the pulp-dentin border from 1 week after replantation. And tertiary dentin was increased at 2 weeks after replantation. The presence of odontoblast-like cells and the formation of tertiary dentin were continued to 4 weeks after replantation. At 4 weeks after replantation. the deposition of bone-like tissues and cementum-like tissues was observed. This results show that there is a possibility of pulp healing after tooth replantation in rats and the mineralization of tooth can progress. The mineralization of tooth after replantation was initially occurred by the deposition of tertiary dentin. but as time passed. the deposition of bone-like tissues and cementum-like tissues was begun and increased.

신경섬유종증 환자에서 상완신경총 병변의 소견을 보인 척추 신경섬유종

양충용,박효인,박순아,박종태,정선관,신용일 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

본 증례는 50세 여성의 제 1형 신경섬유종 환자로서 척추 신경섬유종에 의해 초기 상완신경총 병변 소견을 보였다. 약 4개월 동안 후경부통, 좌측 견관절, 좌측 상완의 마비를 보였으며, 초기 전기진단학적 검사상 경수 5번과 6번 신경근을 중심으로 쇄골상부 신경절전성 상완신경총 병변이 관찰되었다. 그러나, 액와 자기공명영상, 초음파검사, 단순 방사선검사, 골주사 등의 영상학적 검사에서는 심한 흉추 척추 전-측만증과 경수 뇌경막에 확장 소견을 제외하고는 상완신경총 병변의 소견은 관찰되지 않았다. 7개월경과 후, 좌측 상지 및 양측 하지의 근력 저하, 경수 4번이하의 감각 저하, 경도의 흉통을 동반한 호흡곤란이 진행되었다. 응급으로 시행된 경수 자기공명영상에서 좌측 경수 3번과 4번에 경막내 액와-속질의 신경종양이 진단되었다. 수술적 처치를 시행하였으며, 거시적 관찰상 척수 신경섬유종이 진단되었다. 급격히 진행하는 척수 신경섬유종은 초기에 상완신경총의 신경근 병변의 소견을 보일 수 있으므로, 신경섬유종증 환자에서 상완신경총 병변의 진단에 세심한 주의를 요한다. We observed a patient with symptom similar to brachial plexus lesion caused by spinal neurofibroma in a 50-year-old female with a history of neurofibromatosis-1(NF-1). She presented with posterior neck, left shoulder and left upper arm paralysis of 4 months duration. The initial electrodiagnostic study(EDS) revealed supraclavicular preganglionic brachial plexus lesion of mainly involved left C5 and C6 roots. However, radiological imagings including axillar MRI, ultrasonography, cervical plain radiography and bone scintigraphy showed no definitive brachial plexus disease except cervical dural dilation and severe thoracic scolio-Iordosis. On 7 months after the first admission, she had aggravated muscle weakness on the left upper arm and both lower extremities, sensory impairment below C4 and respiration difficulty with the chest pain. Emergency MRI showed intradural axillar-medullary neurogenic tumor at the left C3-C4. Subsequently, the patient was treated with surgical resection. Grossly specimen examination displayed the presence of a spinal neurofibroma. In rapidly progressing spinal neurofibroma represented firstly as root lesion of brachial plexus in EDS, the physician should pay scrupulous care to evaluate the cause.

꺅도요류의 도래현황과 외형적 특징에 관한 연구

박종길(Jong-Gil Park),원일재(II-Jae Won),채희영(Hee-Young Chae) 한국조류학회II 2007 한국조류학회지 Vol.14 No.2

본 조사는 꺅도요류의 도래현황과 종간 외형적 차이를 파악하기 위해 2007년 3월부터 10월까지 대흑산도 북쪽에 위치한 배낭기미습지에서 실시하였다. 조사 결과 꺅도요(Gallingo gallinago), 바늘꼬리도요(G. stenura), 꺅도요사촌(G. megala)의 3종 555개체(봄 217개체, 가을 338개체)가 관찰되었으며, 이중 3종 113개체를 포획 조사하였다. 포획 개체의 종 구성은 꺅도요 51개체, 바늘꼬리도요 46개체, 꺅도요사촌 16개체였다. 꺅도요류 3종의 통과시기에 있어서 종간 뚜렷한 차이를 보였다. 봄철 꺅도요는 3월 13일 처음 모습을 보였으며, 꺅도요사촌은 1개월 후인 4월 12일에, 바늘꼬리도요는 4월 25일에 관찰되었다. 남하시기에는 바늘꼬리도요가 8월 8일 모습을 보이기 시작하였고, 8월 22일에 꺅도요가 그 뒤를 이었으며, 8월 23일에 꺅도요사촌이 도착하였다. 꺅도요사촌과 바늘꼬리도요는 봄·가을 개체수에서 큰 차이가 있었다. 바늘꼬리도요는 봄 북상시기에 19개체가 통과하였지만 가을 남하시기에는 134개체가 통과하였다. 반대로 각도요사촌은 봄에 56개체가 통과한 반면, 가을에는 8개체가 통과하였다. 지금까지 알려진 바와 달리 꺅도요사촌 수컷은 20장의 꼬리깃을 가지며, 암컷은 18장을 가지는 경우가 많았다. 또한 수컷의 꼬리 길이가 암컷보다 뚜렷하게 길어 큰꺅도요-(G. hardwickii)와 거의 같은 길이로 나타났다. 이같은 차이는 꺅도요사촌이 성적 이형이 나타날 수 있음을 보였다. 18장의 꼬리깃을 가지는 꺅도요사촌 암컷은 크기가 수컷보다 크고, 외형적 특성으로 인하여 가락지 부착조사에서 조차도 큰꺅도요와 구별이 매우 어려웠다. 결과적으로 외형적인 특징, 꼬리깃 수만으로 꺅도요사촌과 큰꺅도요의 구별은 불가능하고, 꼬리깃 형태와 측정자료 비교, 분석을 통해서 종 동정이 가능한 것으로 나타났다. 또한 큰꺅도요가 포획되지 않아 한반도 서부에서는 희귀하게 통과하거나, 일부 지역에 한하여 통과할 가능성도 있으므로 국내 큰꺅도요의 분포권, 도래실태, 동정방법에 대한 재조명이 요구된다. Specific differences in timing of migration and morphological difference among four Snipes were monitored at Baenanggimi reed-bed, northern part of Heuksando Island (34˚41' N, 125˚25' E) from early May to late October 2007. We monitored a total of 555 individuals (217 in spring and 338 in autumn) of three snipe species the Common Snipes (Gallingo gallinago), the Pintail Snipes (G. stenura), and the Swinhoe's Snipes (G. megala) and ringed 111 individuals (51 Common, 46 Pintail, and 16 Swinhoe's Snipe) by mist-netting survey. The timing of migration was significantly different among three species. In Spring, the first observation of the Common Snipe was on 13th March, about one month earlier than that of the Swinhoe's and the Pintail Snipes. The Swinhoe's and the Pintail Snipes were seen on 12th April and on 25th April, respectively. In Autumn, the Pintail Snipe was firstly observed on 8th August, the Common Snipe on 22th August and the Swinhoe's Snipe on 23th August. The Common Snipes stayed until early November and then all moved away. The population sizes of the Pintail and the Swinhoe's Snipes were different between spring and autumn migration. In spring the Pintail Snipe were not common, but it was abundant as the Common Snipe in autumn. Conversely, the Swinhoe's Snipe were common in spring and rare in autumn. Generally, the male Swinhoe's Snipe had 20 rectrices and the female had 18. The rectrices of male were significantly longer than those of female, and were almost same length as those of the Latham's Snipe (G. hardwickii). The data from this study were very different from those of Prater (1977) and Hayman et al.(1986). We suggest that the Swinhoe's Snipe has sexual dimorphism like the Latham's Snipe. There is reasonable possibility of mis-identification between the Swinhoe's Snipe with 18 rectrices and the Latham's Snipe even in hands. Actually, the identification was very difficult both in fields and hands. So, the patterns of rectrices, numbers of rectrices and measurements data were examined for correct identification in the lab. According to our results, the Latham's Snipe are probably a very rare passage migrant, at least in western parts of Korea. More intensive surveys on its distribution, status and identification are still needed in fields.