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Kim, Young-Jon,Kim, Byoung-Ryun,Ryu, Jae-Suk,Lee, Gyeong-Ok,Kim, Hak-Ryul,Choi, Keum-Ha,Ryu, Jae-Won,Na, Kyoung-Suk,Park, Min-Cheol,So, Hong-Seob,Cho, Ji-Hyun,Park, Do-Sim Blackwell Scientific Publications 2017 International journal of gynecological cancer Vol.27 No.2
<B>Objective</B><P>Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), serine/arginine-rich splicing factor 1 (SRSF1), and SRSF3 are splicing regulators associated with oncogenesis. However, the alterations of SF proteins and their diagnostic values in cervical cancer are unclear. To apply SFs clinically, effective marker selection and characterization of the target organ properties are essential.</P><B>Materials and Methods</B><P>We concurrently analyzed HNRNPA1, SRSF1, SRSF3, and the conventional tumor markers squamous cell carcinoma antigen (SCCA) and carcinoembryonic antigen (CEA) in cervical tissue samples (n = 127) using semiquantitative immunoblotting. In addition, we compared them with p16 (cyclin-dependent kinase inhibitor 2A [CDKN2A]), which has shown high diagnostic efficacy in immunohistochemical staining studies and has been proposed as a candidate protein for point-of-care screening biochemical tests of cervical neoplasia.</P><B>Results</B><P>HNRNPA1, higher molecular weight forms of SRSF1 (SRSF1-HMws), SRSF3, CEA, and p16 levels were higher (<I>P</I> < 0.05) in cervical carcinoma tissue samples than in nontumoral cervical tissue samples. However, the levels of SRSF1-Total (sum of SRSF1-HMws and a lower molecular weight form of SRSF1) and SCCA, a commonly used cervical tumor marker, were not different between carcinoma and nontumoral tissue samples. In paired sample comparisons, HNRNPA1 (94%) showed the highest incidence of up-regulation (carcinoma/nontumor, >1.5) in cervical carcinoma, followed by p16 (84%), SRSF1-HMws (69%), SRSF3 (66%), CEA (66 %), SCCA (32%), and SRSF1-Total (31%). HNRNPA1 (92%) and p16 (91%) presented the two highest diagnostic accuracies for cervical carcinoma, which were superior to those of SRSF3 (75%), SRSF1-HMws (72%), CEA (72%), SCCA (59%), and SRSF1-Total (55%).</P><B>Conclusions</B><P>Our results identified that HNRNPA1 is the best diagnostic marker among the SFs and conventional markers given its excellent diagnostic efficacy for cervical carcinoma, and it has a p16-comparable diagnostic value. We suggest that HNRNPA1 is an additional effective target protein for developing cervical cancer detection tools.</P>
Lee, Cheol Whan,Suh, Jon,Lee, Se-Whan,Park, Duk-Woo,Lee, Seung-Hwan,Kim, Young-Hak,Hong, Myeong-Ki,Kim, Jae-Joong,Park, Seong-Wook,Park,, Seung-Jung WILEY-LISS 2007 CATHETERIZATION AND CARDIOVASCULAR INTERVENTIONS Vol.69 No.6
<B>Objectives:</B><P>Predictors of cardiac events and restenosis after sirolimus-eluting stent (SES) implantation in small coronary arteries were evaluated.</P><B>Background:</B><P>Although SES implantation has markedly reduced the risk of restenosis, small vessel disease remains a major cause of SES failure.</P><B>Methods:</B><P>We prospectively investigated the factors predictive of cardiac events and restenosis in 1,092 consecutive patients who received SES implantation for 1,269 lesions in small coronary arteries (≤2.8 mm). Follow-up angiography at 6 months was performed in 751 patients with 889 lesions (follow-up rate 70.3%).</P><B>Results:</B><P>Restenosis (diameter stenosis ≥ 50%) was angiographically documented in 65 patients with 77 lesions (8.7%): 55 focal (71.4%), 8 diffuse (10.4%), 2 diffuse proliferative (2.6%), and 12 total (15.6%). Lesion length, stent length, reference artery size, and in-stent restenotic lesions were univariate predictors of restenosis. By multivariate analysis, lesion length (OR 1.04; 95% CI 1.02–1.05; P < 0.001) and in-stent restenotic lesions (OR 3.38; 95% CI 1.80–6.35; P < 0.001) were significant independent predictors of restenosis. During follow-up (23.2 ± 7.9 months), there were 17 deaths (5 cardiac and 12 noncardiac), 5 nonfatal Q-wave myocardial infarctions, and 42 target lesion revascularizations. The cumulative probability of survival without major adverse cardiac events (MACE) was (96.6 ± 0.6)% at 1 year and (95.1 ± 0.7)% at 2 years. In multivariate analysis, lesion length (HR 1.04; 95% CI 1.01–1.07; P = 0.004) and in-stent restenotic lesions (HR 3.29; 95% CI 1.58–6.86; P = 0.001) were independently related to MACE.</P><B>Conclusions:</B><P>SES implantation in small coronary arteries is safe and effective, with lesion length having a major impact on restenosis and MACE. © 2006 Wiley-Liss, Inc.</P>
Tong Il Kim,Hak Sung Yun,Yung Jon,Gwang Bok Han,Sung Il Chae,Ryong Nam An 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.4
In this work, flower-like γ-AlOOH and γ-Al2O3 nanoarchitectures with excellent surface and mesoporous properties were successfully synthesized in ionic liquid-assisted hydrothermal reaction system. The as-prepared suprastructures were characterized by several techniques, such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and nitrogen adsorption/desorption measurement. The formation mechanism of flower-like γ-AlOOH mesoporous crystallites by ionic liquid-assisted hydrothermal process was proposed and discussed. Flower-like γ-Al2O3 nanostructures were obtained by calcining the as-prepared γ-AlOOH at 600 ℃ for 2 h, preserving the same morphology.
Whan Lee, Cheol,Kim, Sang-Hyun,Suh, Jon,Park, Duk-Woo,Lee, Seung-Hwan,Kim, Young-Hak,Hong, Myeong-Ki,Kim, Jae-Joong,Park, Seong-Wook,Park, Seung-Jung Wiley Subscription Services, Inc., A Wiley Company 2008 CATHETERIZATION AND CARDIOVASCULAR INTERVENTIONS Vol.71 No.5
<B>Background:</B><P>Sirolimus-eluting stents have been increasingly used for treatment of restenosis after implantation of bare metal stents (BMSs) or drug-eluting stents (DESs), but little is known regarding their long-term outcomes.</P><B>Methods:</B><P>We compared long-term clinical outcomes in 295 patients treated with sirolimus-eluting stents for post-BMS (n = 224) vs. post-DES (n = 71) restenosis. All follow-ups were at least 12 months, and the primary endpoint was major adverse cardiac events (MACE), defined as cardiac death, nonfatal myocardial infarction (MI) or target lesion revascularization (TLR).</P><B>Results:</B><P>Baseline characteristics were similar between the two groups, except that mean lesion length (28.0 ± 16.2 vs. 19.5 ± 13.6, P < 0.01) and mean stented length (35.4 ± 19.2 vs. 25.7 ± 14.7, P < 0.01) were significantly longer in the post-BMS group. Major in-hospital complications occurred in 2 patients. During a mean follow-up of 31.3 ± 11.1 months, there were 9 deaths (4 cardiac, 5 noncardiac), 3 nonfatal MIs, and 25 TLRs. Late stent thrombosis was documented in 2 patients (1 in each group). There were no between group differences in cardiac or total deaths, but there were trends toward less frequent cardiac death/MI or TLR in the post-BMS group. The cumulative probability of MACE-free survival was significantly better for the post-BMS group (95.0% ± 1.5% vs. 87.3% ± 4.0% at 1 year; 93.0% ± 1.7% vs. 81.0% ± 5.2% at 2 years; Log Rank P = 0.016). In multivariate analysis, post-DES restenosis was the only significant predictor of MACE (OR 3.29, 95%CI 1.13–9.61, P = 0.029).</P><B>Conclusions:</B><P>Sirolimus-eluting stents were effective for treatment of in-stent restenosis, but post-DES restenosis was associated with poorer outcomes than post-BMS restenosis. © 2008 Wiley-Liss, Inc.</P>
소형시험편의 Master Curve 방법을 이용한 원자로 압력용기강의 파괴인성 천이특성평가
양원존,허무영,김주학,이봉상,홍준화,Yang, Won-Jon,Heo, Mu-Yeong,Kim, Ju-Hak,Lee, Bong-Sang,Hong, Jun-Hwa 대한기계학회 2000 大韓機械學會論文集A Vol.24 No.2
Fracture toughness of five different reactor pressure vessel steels was characterized in the transition temperature region by the ASTM E1921-97 standard method using Charpy-sized small specimens. T he predominant fracture mode of the tested steels was transgranular cleavage in the test conditions. A statistical analysis based on the Weibull distribution was applied to the interpretation of the scattered fracture toughness data. The size-dependence of the measured fracture toughness values was also well predicted by means of the Weibull probabilistic analysis. The measured fracture toughness transition curves followed the temperature-dependence of the ASTM master curve within the expected scatter bands. Therefore, the fracture toughness characteristics in the transition region could be described by a single parameter, so-called the reference temperature (T。), for a given steel. The determined reference temperatures of the tested materials could not be correlated with the conventional index temperatures from Charpy impact tests.
Polymer Thin Film–Induced Tumor Spheroids Acquire Cancer Stem Cell–like Properties
Choi, Minsuk,Yu, Seung J.,Choi, Yoonjung,Lee, Hak R.,Lee, Eunbeol,Lee, Eunjung,Lee, Yumi,Song, Junhyuk,Son, Jin G.,Lee, Tae G.,Kim, Jin Y.,Kang, Sukmo,Baek, Jieung,Lee, Daeyoup,Im, Sung G.,Jon, Sangyo American Association for Cancer Research 2018 Cancer Research Vol.78 No.24
<P>A new cell culture technology enables highly tumorigenic 3D spheroids to be easily generated from various cancer cell sources in the common laboratory.</P><P><B></B></P><P>Although cancer stem cells (CSC) are thought to be responsible for tumor recurrence and resistance to chemotherapy, CSC-related research and drug development have been hampered by the limited supply of diverse, patient-derived CSC. Here, we present a functional polymer thin film (PTF) platform that promotes conversion of cancer cells to highly tumorigenic three-dimensional (3D) spheroids without the use of biochemical or genetic manipulations. Culturing various human cancer cells on the specific PTF, poly(2,4,6,8-tetravinyl-2,4,6,8-tetramethyl cyclotetrasiloxane) (pV4D4), gave rise to numerous multicellular tumor spheroids within 24 hours with high efficiency and reproducibility. Cancer cells in the resulting spheroids showed a significant increase in the expression of CSC-associated genes and acquired increased drug resistance compared with two-dimensional monolayer-cultured controls. These spheroids also exhibited enhanced xenograft tumor-forming ability and metastatic capacity in nude mice. By enabling the generation of tumorigenic spheroids from diverse cancer cells, the surface platform described here harbors the potential to contribute to CSC-related basic research and drug development.</P><P><B>Significance:</B></P><P>A new cell culture technology enables highly tumorigenic 3D spheroids to be easily generated from various cancer cell sources in the common laboratory.</P>