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Jin, Xun,Beck, Samuel,Sohn, Young-Woo,Kim, Jun-Kyum,Kim, Sung-Hak,Yin, Jinlong,Pian, Xumin,Kim, Sung-Chan,Choi, Yun-Jaie,Kim, Hyung-Gee Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.8
Although human telomerase catalytic subunit (TERT) has several cellular functions including telomere homeostasis, genomic stability, cell proliferation, and tumorigenesis, the molecular mechanism underlying anti-apoptosis regulated by TERT remains to be elucidated. Here, we show that ectopic expression of TERT in spontaneously immortalized human fetal fibroblast (HFFS) cells, which are a telomerase- and p53-positive, leads to increases of cell proliferation and transformation, as well as a resistance to DNA damage response and inactivation of p53 function. We found that TERT and a mutant TERT (no telomerase activity) induce expression of basic fibroblast growth factor (bFGF), and ectopic expression of bFGF also allows cells to be resistant to DNA-damaging response and to suppress activation of p53 function under DNA-damaging induction. Furthermore, loss of TERT or bFGF markedly increases a p53 activity and DNA-damage sensitivity in HFFS, HeLa and U87MG cells. Therefore, our findings indicate that a novel TERT-bFGF axis accelerates the inactivation of p53 and consequent increase of resistance to DNA-damage response.
Jin, Xun,Lee, Joong-Seub,Kwak, Sungwook,Jung, Ji-Eun,Kim, Tae-Kyung,Xu, Chenxiong,Hong, Zhongshan,Li, Zhehu,Kim, Sun-Myoung,Whang, Kwang Youn,Hong, Ki-Chang,You, Seungkwon,Choi, Yun-Jaie,Kim, Hyunggee Korean Society for Molecular Biology 2006 Molecules and cells Vol.21 No.2
<P>We have established in culture a spontaneously immortalized bovine embryonic fibroblast (BEF) cell line that has lost p53 and p16(INK4a) functions. MyoD is a muscle-specific regulator capable of inducing myogenesis in a number of cell types. When the BEF cells were transduced with MyoD they differentiated efficiently to desmin-positive myofibers in the presence of 2% horse serum and 1.7 nM insulin. The myogenic differentiation of this cell line was more rapid and obvious than that of C2C12 cells, as judged by morphological changes and expression of various muscle regulatory factors. To confirm that lack of the p53 and p16(INK4a) pathway does not prevent MyoD-mediated myogenesis, we established a cell line transformed with SV40LT (BEFV) and introduced MyoD into it. In the presence of 2% horse serum and 1.7 nM insulin, the MyoD-transduced BEFV cells differentiated like the MyoD-transduced BEFS cells, and displayed a similar pattern of expression of muscle regulatory proteins. Taken together, our results indicate that MyoD overexpression overcomes the defect in muscle differentiation associated with immortalization and cell transformation caused by the loss of p53 and Rb functions.</P>
Jin Xun,Guo Xingle,Chen Jian,Li Jiasheng,Zhang Shufei,Zheng Sixu,Wang Yunpeng,Peng Ying,Zhang Kun,Liu Yifan,Liu Bingjian 한국유전학회 2023 Genes & Genomics Vol.45 No.3
Background In this study, the complete mitogenome of Hemigrapsus sinensis was the first identified and analyzed. Objective The complete mitochondrial genome of Hemigrapsus sinensis (Brachyura, Grapsoidea, Varunidae) and its phylogenetic position within Grapsoidea. Methods The sample of Hemigrapsus sinensis was collected and DNA was extracted. After sequencing, NOVOPlasty was used for sequence assembly. Annotate sequences with MITOS WebServer, tRNAscan-SE2.0, and NCBI database. MEGA was used for sequence analysis and Phylosuite was used for phylogenetic tree construction. DnaSP was used to calculate Ka/Ks. Results This mitochondrial genome shows that it was 15,900 bp and encoded 13 PCGs, 22 tRNA genes, two rRNA genes, and one control region. The genome composition tends to A + T (74.34%) and presents a negative GC-skew (− 0.22) and AT-skew (− 0.03). The PCGs initiation codon was the typical ATN and termination codon was the typical TAN, incomplete T or missing. The ML and BI trees showed that H. sinensis was most closely related to Hemigrapsus and clustered together with the Varunidae. And our phylogenetic trees provide proof that Ocypodoidea and Grapsoidea may be of common origin. Meanwhile, in the phylogenetic tree, parallel mixing of Chiromantes and Orisarma raised doubts over the traditional classification system. Besides, Incomplete Lineage sorting (ILS) was observed in Varunidae. In the subsequent analysis of evolution rate, we found that all of the PCGs (NAD4 was not calculated) had undergone negative selections, indicating the conservation of mitochondrial genes of H. sinensis during the evolution. Conclusion Therefore, researching the complete mitogenome of H. sinensis would be contributing to molecular taxonomy, phylogenetic relationship, and breeding optimization within the Grapsoidea superfamily.
장련(Zhang, Lian),이경훈(Li, Jinxun),이용진(Li, Longzhen) 글로벌경영학회 2015 글로벌경영학회지 Vol.12 No.4
기업 경영성과 관련 연구에서 지식기반이론과 사회연결망 (사회자본)관점을 결합하여 분 석하는 것이 바람직하다. 따라서 본 연구는 사업연줄 (business ties)과 정치적 연줄 (political ties)이 모회사 지식이전과 현지지식 획득이 중국 진출 한국 자회사의 성과에 미치는 영향에 대한 조절작 용에 대해 분석을 진행하였다. 이는 현지 경영연줄(managerial ties)의 형성과 유지의 진정한 작용을 명확히 하는데 중요한 의미가 있다. 본 연구의 결과에 의하면 경영자의 사업 및 정치적 연줄은 현지 지식획득과 자회사의 성과의 관계에 정의 조절작용을 일으키는 반면 사업연줄은 모회사 지식이전과 자회사 성과의 관계에 부의 조절작용을 일으키게 된다. The knowledge-based view and network along with social capital views were argued to be necessary to be taken together in investigating firm performance. In line with this suggestion, the purpose of this study is to clarify the effect of business and political ties for the influence of headquarters knowledge transfer and local knowledge acquisition on Korean subsidiary performance in China. In turn, this will make us to identify the authentic roles of establishing and sustaining managerial ties locally. Our findings illustrate that executives business and political ties have positive moderating effect on the relationship between local knowledge acquisition and subsidiary performance while business ties show negative interactive effect with headquarters knowledge transfer on subsidiary performance.
Yangyang Guo,Bin Xu,Ying Liu,Wenzhong Yang,Xiaoshuang Yin,Yun Chen,Jinxun Le,Zhihao Chen 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.56 No.-
The ionic liquids, 1-vinyl-3-aminopropylimidazolium hexafluorophosphate ([VAIM][PF6]) and 1-vinyl-3-aminopropylimidazolium tetrafluoroborate ([VAIM][BF4]) acted as the corrosion inhibitors. Weight lossmeasurements showed the corrosion inhibition efficiencies of [VAIM][PF6] and [VAIM][BF4] were 90.53%and 54.01% at 45 C, respectively. The ILs were mix-type inhibitors. Raising the temperature can have adecreased inhibition efficiency of [VAIM][BF4], while [VAIM][PF6] presented an opposite trend. Inaddition, [VAIM][PF6] obeyed Langmuir monolayer adsorption isotherm, while [VAIM][BF4] obeyed EIAwadykinetic-thermodynamic adsorption for blocking active sites. Moreover, Molecular DynamicSimulations showed that [VAIM][PF6] owned a higher adsorption energy, which may be responsible forthe more adsorption groups of [VAIM][PF6].