<i>Hymenobacter ruber</i> sp. nov., isolated from grass soil

Jin, Long,Lee, Hyung-Gwan,Kim, Song-Gun,Lee, Keun Chul,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.3

<P>A taxonomic study using a polyphasic approach was performed on a Gram-stain-negative, aerobic, non-motile, non-spore-forming, rod-shaped bacterium, designated strain PB156<SUP>T</SUP>, isolated from grass soil. Comparative 16S rRNA gene sequence studies showed that the isolate was clearly affiliated with the phylum <I>Bacteroidetes</I>, and most closely related to <I>Hymenobacter soli</I> PB17<SUP>T</SUP>, <I>Hymenobacter antarcticus</I> VUG-A42aa<SUP>T</SUP> and <I>Hymenobacter glaciei</I> VUG-A130<SUP>T</SUP>, showing 96.4, 96.2 and 95.9 % 16S rRNA gene sequence similarity, respectively, while all other species of the genus shared only 89.3–95.2 % similarity. The main polyamine present was <I>sym</I>-homospermidine. The predominant menaquinone was MK-7. The major fatty acids were C<SUB>15 : 0</SUB> iso, summed feature 3 (C<SUB>16 : 1</SUB>ω6<I>c</I> and/or C<SUB>16 : 1</SUB>ω7<I>c</I>/<I>t</I>), C<SUB>16 : 1</SUB>ω5<I>c</I> and C<SUB>15 : 0</SUB> anteiso. The G+C content of the genomic DNA of strain PB156<SUP>T</SUP> was 61.7 mol%. The combined genotypic and phenotypic data supported the conclusion that strain PB156<SUP>T</SUP> represents a novel species of the genus <I>Hymenobacter</I>, for which the name <I>Hymenobacter ruber</I> sp. nov. is proposed. The type strain is PB156<SUP>T</SUP> ( = KCTC 32477<SUP>T</SUP> = JCM 19433<SUP>T</SUP>).</P>

<i>Caulobacter daechungensis</i> sp. nov., a stalked bacterium isolated from a eutrophic reservoir

Jin, Long,Lee, Hyung-Gwan,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.7

<P>A Gram-stain-negative, aerobic, non-spore-forming, curved, rod-shaped bacterium, H-E3-2<SUP>T</SUP>, was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Strain H-E3-2<SUP>T</SUP> was motile with a single polar flagellum or non-motile (stalked cell). Comparative 16S rRNA gene sequence studies showed the isolate had a clear affiliation with the class <I>Alphaproteobacteria</I> and was most closely related to <I>Caulobacter fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>Caulobacter mirabilis</I> LMG 24261<SUP>T</SUP>, showing 97.6 and 97.3 % 16S rRNA gene sequence similarity, respectively, and 95.3–96.3 % similarity to all other species of the genus <I>Caulobacter</I>. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C<SUB>18 : 1</SUB>ω6<I>c</I> and/or C<SUB>18 : 1</SUB>ω7<I>c</I>) and C<SUB>16 : 0</SUB>. The G+C content of the genomic DNA of strain H-E3-2<SUP>T</SUP> was 64.7 mol%. DNA–DNA hybridization values of strain H-E3-2<SUP>T</SUP> with <I>C. fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>C. mirabilis</I> LMG 24261<SUP>T</SUP> were 21.2 and 19.7 %, respectively. Thus, based on the results of polyphasic analysis, it is proposed that strain H-E3-2<SUP>T</SUP> represents a novel species of the genus <I>Caulobacter</I>, for which the name <I>Caulobacter daechungensis</I> sp. nov. is proposed. The type strain is H-E3-2<SUP>T</SUP> ( = KCTC 32211<SUP>T</SUP> = JCM 18689<SUP>T</SUP>).</P>

<i>Aquihabitans daechungensis</i> gen. nov., sp. nov., an actinobacterium isolated from reservoir water

Jin, Long,Huy, Hangsak,Kim, Kwang Kyu,Lee, Hyung-Gwan,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.8

<P>A novel Gram-reaction-positive bacterium, strain CH22-21<SUP>T</SUP>, was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Cells of strain CH22-21<SUP>T</SUP> were non-motile, ciliated short rods that formed creamy-white colonies on half-strength modified R2A agar. Chemotaxonomic results showed menaquinone MK-9(H<SUB>6</SUB>) as the predominant respiratory menaquinone, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside as major polar lipids, 16 : 1ω5<I>c</I>, 16 : 0, 17 : 1ω8<I>c</I> and 18 : 1ω9<I>c</I> as major fatty acids, and a DNA G+C content of 71.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the order <I>Acidimicrobiales</I>, showing similarity values of <92.3 % with its closest phylogenetic neighbours. The combined genotypic and phenotypic data showed that strain CH22-21<SUP>T</SUP> could be distinguished from all genera within the order <I>Acidimicrobiales</I> and represented a novel species of a new genus in the family <I>Iamiaceae</I>, for which the name <I>Aquihabitans dachungensis</I> gen. nov., sp. nov. is proposed. The type strain of <I>Aquihabitans dachungensis</I> is CH22-21<SUP>T</SUP> ( = KCTC 19849<SUP>T</SUP> = JCM 17787<SUP>T</SUP>).</P>

<i>Flaviflexus salsibiostraticola</i> sp. nov., an actinobacterium isolated from a biofilm reactor

Jin, Long,Ko, So-Ra,Lee, Hyung-Gwan,Kim, Byung-Hyuk,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.9

<P>A Gram-stain-positive, aerobic, non-motile, non-spore-forming, cocci-shaped actinobacterium, designated strain EBR4-1-2<SUP>T</SUP>, was isolated from a biofilm reactor in Korea. Comparative 16S rRNA gene sequence studies showed the isolate was clearly affiliated with the class <I>Actinobacteria</I>, and was related most closely to <I>Flaviflexus huanghaiensis</I> H5<SUP>T</SUP>, showing 98.9 % similarity. Cells of strain EBR4-1-2<SUP>T</SUP> formed yellow colonies on R2A agar, contained MK-9(H<SUB>4</SUB>) as the predominant menaquinone, and included C<SUB>18 : 1</SUB>ω9<I>c</I>, C<SUB>16 : 0</SUB>, C<SUB>16 : 1</SUB>ω9<I>c</I> and C<SUB>14 : 0</SUB> as the major fatty acids. The cell-wall peptidoglycan type was A5α (<SMALL>l</SMALL>-Lys–<SMALL>l</SMALL>-Ala–<SMALL>l</SMALL>-Lys–<SMALL>d</SMALL>-Glu). The G+C content of the genomic DNA of strain EBR4-1-2<SUP>T</SUP> was 65.6 mol%. Thus, the combined genotypic and phenotypic data supported the conclusion that strain EBR4-1-2<SUP>T</SUP> represents a novel species of the genus <I>Flaviflexus</I>, for which the name <I>Flaviflexus</I> <I>salsibiostraticola</I> sp. nov. is proposed. The type strain is EBR4-1-2<SUP>T</SUP> ( = KCTC 33148<SUP>T</SUP> = JCM 19016<SUP>T</SUP>).</P>

<i>Chelatococcus caeni</i> sp. nov., isolated from a biofilm reactor sludge sample

Jin, Long,Ko, So-Ra,Lee, Hyung-Gwan,Kim, Byung-Hyuk,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.3

<P>A polyphasic taxonomic study was carried out on strain EBR-4-1<SUP>T</SUP>, which was isolated from a biofilm reactor in the Republic of Korea. The cells of the strain were Gram-stain-negative, non-spore-forming, motile and rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain to the <I>Alphaproteobacteria</I>, and it was most closely related to <I>Chelatococcus daeguensis</I> CCUG 54519<SUP>T</SUP>, <I>Chelatococcus sambhunathii</I> HT4<SUP>T</SUP>, and <I>Chelatococcus asaccharovorans</I> DSM 6462<SUP>T</SUP> with 16S rRNA gene sequence similarities to the type strains of these species of 98.8 %, 98.7 %, and 96.3 %, respectively. The G+C content of the genomic DNA of strain EBR-4-1<SUP>T</SUP> was 68.7 mol%. Phenotypic and chemotaxonomic data [Q-10 as the major ubiquinone; C<SUB>19 : 0cyclo</SUB>ω8<I>c</I>, C<SUB>18 : 1</SUB> 2-OH, and summed feature 8 (C<SUB>18 : 1</SUB>ω7<I>c</I> and/or C<SUB>18 : 1</SUB>ω6<I>c</I>) as the major fatty acids] supported the affiliation of strain EBR-4-1<SUP>T</SUP> to the genus <I>Chelatococcus</I>. On the basis of the polyphasic evidence, it is proposed that strain EBR-4-1<SUP>T</SUP> should be assigned to a new species, <I>Chelatococcus</I> <I>caeni</I> sp. nov. The type strain is EBR-4-1<SUP>T</SUP> ( = KCTC 32487<SUP>T</SUP> = JCM 30181<SUP>T</SUP>).</P>

Ferruginibacter profundus sp. nov., a novel member of the family Chitinophagaceae, isolated from freshwater sediment of a reservoir

Jin, Long,Lee, Hyung-Gwan,La, Hyun-Joon,Ko, So-Ra,Ahn, Chi-Yong,Oh, Hee-Mock Springer-Verlag 2014 Antonie van Leeuwenhoek Vol.106 No.2

<i>Caulobacter</i> <i>profunda</i> sp. nov., isolated from deep freshwater sediment

Jin, Long,La, Hyun-Joon,Lee, Hyung-Gwan,Lee, Jay Jung,Lee, Sanghyup,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.3

<P>The Gram-stain-negative, aerobic, non-spore-forming, motile, with a single polar flagellum, or non-motile (stalked) and rod-shaped bacteria, DS48-5-2<SUP>T</SUP> and DS48-6-3, were isolated from a sediment sample collected from a depth of 48 m taken from Daechung Reservoir, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the two isolates had clear affiliation with <I>Alphaproteobacteria</I> and the closest relatedness to <I>Caulobacter mirabilis</I> FWC 38<SUP>T</SUP>, <I>Caulobacter fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>Caulobacter daechungensis</I> H-E3-2<SUP>T</SUP> showing 98.5 %, 97.3 % and 97.3 % 16S rRNA gene sequence similarity, respectively, and 96.1–96.7 % similarity to all other species of the genus <I>Caulobacter</I>. The two isolates shared 100 % 16S rRNA gene sequence similarity. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C<SUB>18 : 1</SUB>ω6<I>c</I> and/or C<SUB>18 : 1</SUB>ω7<I>c</I>), C<SUB>16 : 0</SUB>, C<SUB>18 : 0</SUB>ω7<I>c</I> 11-methyl and summed feature 3 (C<SUB>16 : 1</SUB>ω6<I>c</I> and/or C<SUB>16 : 1</SUB>ω7<I>c</I>). The G+C contents of the genomic DNA of strains DS48-5-2<SUP>T</SUP> and DS48-6-3 were 66.7 mol% and 66.2 mol%, respectively. DNA–DNA hybridization values of strains DS48-5-2<SUP>T</SUP> and DS48-6-3 with <I>C. mirabilis</I> FWC 38<SUP>T</SUP>, <I>C. fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>C. daechungensis</I> H-E3-2<SUP>T</SUP> were 19.3 %–24.4 %. Thus, based on the evidence from polyphasic studies, it is proposed that strains DS48-5-2<SUP>T</SUP> and DS48-6-3 are representatives of a novel species in the genus <I>Caulobacter</I>, for which the name <I>Caulobacter</I> <I>profunda</I> sp. nov. is proposed. The type strain is DS48-5-2<SUP>T</SUP> ( = KCTC 32480<SUP>T</SUP> = JCM 19440<SUP>T</SUP>).</P>

Effect of Conversion to CTLA4Ig on Tacrolimus-Induced Diabetic Rats

Jin, Long,Lim, Sun Woo,Jin, Jian,Luo, Kang,Ko, Eun Jeong,Chung, Byung Ha,Lin, Hong Li,Yang, Chul Woo Wolters Kluwer Health, Inc. All rights reserved. 2018 Transplantation Vol.102 No.4

<P>Conclusions CTLA4Ig was not diabetogenic, and conversion to CTLA4Ig reduced TAC-induced pancreatic islet injury.</P>

Use of Lipid Extracts from Various Oil Grains to Supply Dietary Omega-3 Fatty Acids for Dairy Foods - A Preliminary Study

Jin, Long,Sim, Jeong Seok,Song, Kwang-Young,Kim, Dong-Hyeon,Chon, Jung-Whan,Kim, Hyunsook,Seo, Kun-Ho Korean Society of Dairy Science and Biotechnology 2018 Journal of Dairy Science and Biotechnology (JMSB) Vol.36 No.1

The objective of this research was to determine the grain oil most suitable for the production of omega-3 fatty acids (FAs) in China, in order to supply dietary omega-3 FAs. This study focused on the nutritional significance of omega-3 FAs and the wide search of sources of dietary omega-3 FA from oil grains produced in China. Five oil grains produced in the Yanbian region, namely sesame, flax, peanut, soybean, and corn, were selected and analyzed for their fat content and FA composition. Results showed that the lipid content in sesame, flax, and peanut, which was more than 30%, was higher than that in soybean and corn. The polyunsaturated FA content was found to be directly proportional to omega-3 FA content. Flax showed a considerably higher omega-3 FA content (53.43%) than other samples, suggesting its potential as a source of dietary omega-3 FA. The optimal daily intake of flax for males and females was determined to be 2.99 g (over one table spoon) and 2.06 g (nearly one table spoon), respectively. Flax showed the lowest ratio of omega-6 and omega-3 FA (0.32%), which can help reduce this ratio in the human body; the intake of equal amounts of flax and corn could achieve an optimum ratio of 1:1. These results can prove to be valuable in the preparation of various functional dairy foods in the future.

<i>Belnapia soli</i> sp. nov., a proteobacterium isolated from grass soil

Jin, Long,Lee, Hyung-Gwan,No, Kui-Jung,Ko, So-Ra,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.6

<P>A Gram-negative, aerobic, non-motile, non-spore-forming, cocci-shaped, red-pigmented bacterium, designated strain PB-K8<SUP>T</SUP>, was isolated from grass soil sampled in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the isolate was clearly affiliated with the class <I>Alphaproteobacteria</I><I>,</I> and was most closely related to <I>Belnapia moabensis</I> DSM 16746<SUP>T</SUP> and <I>Belnapia rosea</I> DSM 23312<SUP>T</SUP>, showing a 16S rRNA gene sequence similarity to the type strains of each species of 98.4 and 97.2 %, respectively. The cells of strain PB-K8<SUP>T</SUP> formed red colonies on R2A agar, contained Q-9 as the predominant ubiquinone and included summed feature 3 (C<SUB>16 : 1</SUB>ω7<I>c</I> C<SUB>16 : 1</SUB>ω6<I>c</I>), C<SUB>16 : 0</SUB>, summed feature 8 (C<SUB>18 : 1</SUB>ω7<I>c</I>/C<SUB>18 : 1</SUB>ω6<I>c</I>), C<SUB>18 : 1</SUB> 2-OH and C<SUB>19 : 0</SUB> cyclo ω8<I>c</I> as the major fatty acids. The G+C content of the genomic DNA of strain PB-K8<SUP>T</SUP> was 72.1 mol%. Thus, the combined genotypic and phenotypic data supported the conclusion that strain PB-K8<SUP>T</SUP> represents a novel species of the genus <I>Belnapia</I>, for which the name <I>Belnapia soli</I> sp. nov. is proposed. The type strain is PB-K8<SUP>T</SUP> ( = KCTC 23765<SUP>T</SUP> = JCM 18033<SUP>T</SUP>).</P>