Effects of rhizosphere microbiome on the fruit of Cinnamomum migao H. W. Li: culture, amplicon sequencing, and metagenomic sequencing

Chen Jingzhong,Liu Jiming,Liao Xiaofeng 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.5

Cinnamomum migao H. W. Li is an evergreen tree belonging to the Lauraceae family and is endemic to China. Previous studies have found that the rhizosphere microbiome associated with the C. migao wild population plays an essential role in the production of fruit chemical components. However, it remains unknown whether the rhizosphere microbiome aff ects the production of fruit chemical compounds in cultivated C. migao population. Herein, we studied a 10-year-old fruit-bearing population of C. migao and used techniques such as culturing, amplicon sequencing, and metagenomic sequencing to explore the changes in the rhizosphere microbial community structure over three periods. Meanwhile, the molecular ecological network of the rhizosphere microbiome was constructed based on random matrix theory. The unidentifi ed species were found to belong to fungi and Capnodiales. Sphingomonas sp. mm-1 and Streptomyces scabiei in network hubs were recognized in over three fruiting periods. Further, four network hubs were signifi cantly related to fruit chemical compounds production in C. migao . The higher the number of species annotated, the better the explanation for fruit chemical compounds production in C. migao . The rhizosphere microbiome was found to exert a synergistic eff ect by increasing fruit chemical component production in C. migao . This was evinced through KEGG analysis, which revealed the diff erent metabolic activities aff ected by the rhizosphere microbiome. This study revealed the potential ways and putative keystone taxa of rhizosphere microbiome aff ecting fruit component production in C. migao , which opens up new opportunities for further manipulation and development of the rhizosphere microbiome to promote plant productivity.

Two Arbuscular Mycorrhizal Fungi Alleviates Drought Stress and Improves Plant Growth in Cinnamomum migao Seedlings

( Xiaofeng Liao ),( Jingzhong Chen ),( Ruiting Guan ),( Jiming Liu ),( Qinwen Sun ) 한국균학회 2021 Mycobiology Vol.49 No.4

Cinnamomum migao plants often face different degrees of drought in karst habitats, which can lead to plants’ death, especially in the seedling stage. Widespread of arbuscular mycorrhizal (AM) fungi in karst soils have the potential to address this drought, which is a threat to C. migao seedlings. We inoculated C. migao seedlings with spores from Glomus lamellosum and Glomus etunicatum, two AM fungi widely distributed in karst soils, to observe seedling growth response after simulated drought. Our results showed that 40 g of G. lamellosum and G. etunicatum significantly promoted the growth of C. migao seedlings, 120 days after inoculation. Following a 15-day drought treatment, root colonization of the seedlings with G. lamellosum or G. etunicatum had lower the accumulation of malondialdehyde (MDA) and increased the accumulation of enzymes and osmotic substances in the seedlings. The relative water content in different organs (roots, stems, and leaves) of the drought-stressed seedlings was higher in plants with G. lamellosum or G. etunicatum than in plants without AM fungi colonization. Our results showed that inoculation with AM fungi was an effective means to improve the drought resistance of C. migao seedlings.

Palynological implications for Late Glacial to middle Holocene vegetation and environmental history of the Lop Nur Xinjiang Uygur Autonomous Region, northwestern China

Jia, Hongjuan,Wang, Jingzhong,Qin, Xiaoguang,Yi, Sangheon Elsevier 2017 QUATERNARY INTERNATIONAL Vol.436 No.1

<P><B>Abstract</B></P> <P>Lop Nur is located in the northeastern area of the Tarim Basin, in the Xinjiang Uygur Autonomous Region, northwestern China. A 210-cm-deep trench section was collected from the center of the lake. Five accelerator mass spectrometry (AMS) <SUP>14</SUP>C dating results indicate that the studied section encompasses the Late Glacial to middle Holocene periods (12.8–5.5 cal ka BP). Vegetation and environmental changes in the Lop Nur region can be divided into three stages and six sub-stages, based on significant changes in the pollen assemblages. Dry conditions and desert steppe or steppe vegetation dominated this region from 12.8 to 11.4 cal ka BP. The climate became warmer and wetter at the beginning of the Holocene, and steppe vegetation replaced the previous flora. Steppe vegetation continued to dominate until the middle Holocene (8.7–6.7 cal ka BP), which had the wettest moisture conditions. Increased winter temperatures from 6.7 to 5.5 cal ka BP triggered more evaporation, causing shrinkage of the lowland wetlands. Climate events such as the Allerød oscillation, the Younger Dryas, and events at 9.4 cal ka BP, 8.4cal ka BP, 7.5 cal ka BP, and 7.0 cal ka BP, were recognized in the Lop Nur section. The evidence indicates that climate oscillations in the Lop Nur area were influenced and controlled by changes in global climate.</P>

A Complex Network of Well Log based on Visibility Graph

Xiao Li,Chen lixue,Xiao jingzhong 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.2

Research on surface defect detection system and method of train bearing cylindrical roller based on surface scanning

Yudong Bao,Zhentao Zhou,Siming Wei,Jingzhong Xiang 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.9

Aiming at the detection of surface defects of train bearing rollers, a set of surface defect detection systems for bearing cylindrical rollers based on machine vision is designed in this paper, and the vibration response of the roller during the rolling process is simulated and analyzed. The surface image of the cylindrical roller is obtained by using the area scan camera, and the region of interest is extracted by using edge extraction and template matching algorithm to obtain the cylindrical roller image without background interference. The roller cylinder images are spliced by feature matching and regional fusion methods, and the roller surface defects are extracted by OTSU threshold segmentation, Canny operator edge detection and morphological processing, so as to mark the defects for distinction and identification. The method achieves the purpose of quickly identifying the roller surface defects and rejecting the cylindrical rollers with defective surfaces in train bearings. It provides a theoretical basis for the automation and intelligence of the cylindrical rollers of train bearings in the process of maintenance and repair.

Genomic, evolutionary and expression profile analysis of Hsp70 superfamily in A and D genome of cotton (Gossypium spp.) under the challenge of Verticillium dahliae

Songhua Xiao,Jianwen Xu;Jun Zhao;Jianguang Liu,Jianwen Xu;Jun Zhao;Jianguang Liu,Qiaojuan Wu,Jingzhong Yu,Deyue Yu 한국식물학회 2017 Journal of Plant Biology Vol.60 No.1

In this study, we comparatively analyzed the 115 Hsp70 genes identified in Gossypium raimondii, Gossypium hirsutum and Gossypium arboreum genomes. Those Hsp70 genes unequally distributed among chromosomes in A and D genome of cotton (Gossypium spp.), and were classified into 29 groups according to the homology of them. Based on the localization information of the orthologs in Arabidopsis, the Hsp70 proteins were predicted to locate in cytosol, endoplasmic reticulum, mitochondrion or chloroplast. Homologous analysis indicated the evolutionary conservation of Hsp70 in cotton. In addition, those Hsp70 genes were differently expressed in Suyuan-045, Hai-7124 and TM-1, which were highly resistant, resistant, and sensitive to Verticillium dahliae respectively. The expressions of 26 Hsp70 genes were induced by Verticillium dahliae except for Hsp70-07/16/25/26, and the result suggested the potential involvement of them in responding to Verticillium wilt. Hsp70-08/30/31 was highly expressed in both Suyuan-045 and Hai-7124, and it was hypothesized that they might be involved in the resistance to the invasion of Verticillium dahliae. 144h after inoculation with Verticillium dahliae, the expression of Hsp70-13/14/15 was only up-regulated in Suyuan-045, and it was assumed that they might be involved in resistance to the extension of Verticillium dahliae. Further study on those Hsp70 genes would be valuable to reveal the role of them in Verticillium wilt resistance.