Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct

Jing Guo,Qiong Wu,Hongjuan He,Qi Liu,Fengwei Zhang,Jie Lv,Tiebo Zeng,Ning Gu 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.10

Most imprinted genes are concerned with embryonic development, especially placental development. Here, we identified a placenta-specific imprinted gene Qpct. Our results show that Qpct is widely expressed during early embryonic development and can be detected in the telecephalon, midbrain, and rhombencephalon at E9.5-E11.5. Moreover, Qpct is strikingly expressed in the brain, lung and liver in E15.5. Expression signals for Qpct achieved a peak at E15.5 during placental development and were only detected in the labyrinth layer in E15.5 placenta. ChIP assay results suggest that the modification of histone H3K4me3 can result in maternal activating of Qpct.

Cytotoxic activities of chemical constituents from rhizomes of Anemarrhena asphodeloides and their analogues

Jing Guo,Chenghui Xu,Rui Xue,Weixin Jiang,Bin Wu,Chenggang Huang 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5

Seven steroidal saponins (1–7) and two xanthones(8, 9) were isolated from the rhizomes of Anemarrhenaasphodeloides. Then in order to discover moreanalogues, which may possess good biological activity, thestructural modifications of 2 and 9 were performed by acidhydrolysis and acetylation. Consequently, one novel steroidalsaponin (2d, timosaponin BII-d), three compounds(2c, 2e and 2f) which were also the new products preparedby the diluted acid hydrolysis of 3 by our group previously,and four known compounds (2a, 2b, 9a and 9b) wereobtained. The structures were elucidated by analyses ofNMR and MS data. All the compounds were evaluated fortheir cytotoxicities against BEL-7402, HT-29, HeLa andMDA-MB-468 cell lines in vitro by Sulforhodamine proteincoloration method. Compounds 1, 2, 2b, 4–6, 9a and9b showed certain anti-proliferative activities against thefour cell lines, in which compounds 2, 4 and 9b exhibitedespecially more potent activities. The structure–activityrelationships of these compounds were simply discussed.

Performance analysis of SWIPT-assisted adaptive NOMA/OMA system with hardware impairments and imperfect CSI

Jing Guo,Jin Lu,Xianghui Wang,Lili Zhou 한국전자통신연구원 2023 ETRI Journal Vol.45 No.2

This paper investigates the effect of hardware impairments (HIs) and imperfect channel state information (ICSI) on a SWIPT-assisted adaptive nonorthogonal multiple access (NOMA)/orthogonal multiple access (OMA) system over independent and nonidentical Rayleigh fading channels. In the NOMA mode, the energy-constrained near users act as a relay to improve the performance for the far users. The OMA transmission mode is adopted to avoid a complete outage when NOMA is infeasible. The best user selection scheme is considered to maximize the energy harvested and avoid error propagation. To characterize the performance of the proposed systems, closed-form and asymptotic expressions of the outage probability for both near and far users are studied. Moreover, exact and approximate expressions of the ergodic rate for near and far users are investigated. Simulation results are provided to verify our theoretical analysis and confirm the superiority of the proposed NOMA/OMA scheme in comparison with the conventional NOMA and OMA protocol with/without HIs and ICSI.

Characterization of sulfate-reducing bacteria anaerobic granular sludge and granulometric analysis with grey relation

Jing Guo,Yong Kang 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.9

We constructed a bench-scale up-flow anaerobic sludge reactor to systematically investigate the physicochemical characteristics of sulfate-reducing bacteria (SRB) anaerobic granular sludge and evaluate the granular size by a grey relational analysis. Results indicated that the granulation proportion was improved from 17.9% to 68.7% with the sulfate reduction efficiency larger than 90% under gradually shortened hydraulic retention time (HRT) and increased organic loading. Larger SRB granule sludge showed a higher specific gravity and settling velocity. The seed sludge was negatively charged, and the surface charge decreased with the incremental granular diameter. The maximal hydrophobicity and granulation proportion were 69.9% and 42.4%, respectively, for the granular diameter ranging from 1.5 to 2.5 mm. Extracellular polymeric substance (EPS) of the sludge exhibited the highest ratio of protein to polysaccharide (PN/PS) for the granular diameter in the range of 0.5 to 1.5mm. Based on the grey relational analysis of the SRB anaerobic sludge granulation, the correlation degree of the inherent influencing factors was PN/PS>surface charge> hydrophobicity. The theoretical evaluation would be conducive to granulation control during the potential application.

Crystallographic Characterizations of Eutectic and Secondary Carbides in a Fe-12Cr-2.5Mo-1.5W-3V-1.25C Alloy

Jing Guo,Ligang Liu,Yunli Feng,Sha Liu,Xuejun Ren,Qingxiang Yang 대한금속·재료학회 2017 METALS AND MATERIALS International Vol.23 No.2

In this work, the morphology and structures of the eutectic and secondary carbides in a new high chromiumFe-12Cr-2.5Mo-1.5W-3V-1.25C designed for cold-rolling work roll were systematically studied. The precipitatedcarbides inside the grains and along the grain boundaries were investigated with optical microscope,scanning electron microscopy with energy dispersive spectroscopy, transmission electron microscopy andX-Ray diffraction. Selected area diffraction patterns have been successfully used to identify the crystal formationand lattice constants of the carbides with different alloying elements. The results show that the eutectic carbidesprecipitated contain MC and M2C distributed along the grain boundaries with dendrite feature. The compositionand crystal structure analysis shows that the eutectic MC carbides contain VC and WC with a cubic and hexagonalcrystal lattice structures respectively, while the eutectic M2C carbides predominantly contain V2C and Mo2Cwith orthorhombic and hexagonal crystal lattices respectively. The secondary carbides contain MC, M2C,M7C3 formed along the grain boundaries and their sizes are much larger than the eutectic carbides ones. The secondary M23C6 is much small (0.3-0.5μm) and is distributed dispersively inside the grain. Similar tothe eutectic carbides, the secondary carbides also contain VC, WC, V2C, and Mo2C. M7C3 is hexagonal(Fe,Cr)7C3, while M23C6 is indexed to be in a cubic crystal form.

Knockout of Fatty Acid Synthase induces Endoplasmic Reticulum Stress in Porcine Early Embryos

Jing Guo,Ying-Jie Niu,Kyung-Tae Shin,Nam-Hyung Kim,Xiang-Shun Cui 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2

Fatty acid synthase (FASN) is an enzyme that catalyzes the synthesis of long chain fatty acid. During development, FASN plays a role in growth rather than the energy storage pathways. In this study, we hypothesis that knockout of FASN may affect the early embryonic development through induction of the endoplasmic reticulum (ER) stress. The function of FASN was studied using the Crispr/Cas9 technology. We found that FASN knockout induced ER stress by generation of reactive oxygen species (ROS), further resulted in the activation of adaptive unfolded protein response (UPR), caused the splicing of XBP1. In addition, FASN knockout increased ATF4 and CHOP expression, influenced phosphorylation of PERK. At last, Ca2+ was released from the ER and then taken up by the mitochondrial and influenced mitochondrial function, initiated apoptosis. These results demonstrated that FASN knockout induced generation of ROS, which mediated the activation of UPR via the ER and subsequent apoptosis in porcine early embryos.

A comparison of the fracture resistances of endodontically treated mandibular premolars restored with endocrowns and glass fiber postcore retained conventional crowns

Jing Guo,Zhiming Wang,Xuesheng Li,Chaoyang Sun,Erdong Gao,Hongbo Li 대한치과보철학회 2016 The Journal of Advanced Prosthodontics Vol.8 No.6

PURPOSE This in-vitro study aimed to evaluate the fracture resistances and failure modes of endodontically treated mandibular premolars restored with endocrowns and conventional post-core retained crowns. MATERIALS AND METHODS Thirty mandibular premolars were assigned into three groups (n=10): GI, intact teeth; GE, teeth with endocrowns; GC, teeth with conventional post-core supported crowns. Except for the teeth in group GI, all specimens were cut to 1.5 mm above the cementoenamel junction and endodontically treated. Both endocrowns and conventional crowns were fabricated from lithium-disilicate blocks using a CEREC 3D CAD/CAM unit. All specimens were subjected to thermocycling and then to 45° oblique compressive load until fracture occurred. The fracture resistance and failure mode of each specimen were recorded. Data were analyzed with one-way ANOVA and LSD Post Hoc Test (α=.05). RESULTS The fracture resistances of GE and GC were significantly lower than that of GI (P<.01), while no significant difference was found between GE and GC (P=.702). As of the failure mode, most of the specimens in GE and GC were unfavorable while a higher occurrence of favorable failure mode was presented in GI. CONCLUSION For the restoration of mandibular premolar, endocrown shows no advantage in fracture resistance when compared with the conventional method. Both of the two methods cannot rehabilitate endodontically treated teeth with the same fracture resistances that intact mandibular premolars have.

Family and Parent Correlates of Educational Achievement: Migrant Children in China

Jing Guo 한국사회복지학회 2011 Asian Social Work and Policy Review Vol.5 No.2

Analyzing data drawn from the Beijing Migrant Children Compulsory Education Survey (BMCCES),this study examines the impact of child, family and parent factors on migrant Chinesechildren’s performance in math. The central questions address the differences between theperformance of migrant and non-migrant children in Beijing, the family and parent correlates ofeducation achievement, such as household income and parent education, the impact of social capitalbased on family relations, norms and interactions, such as educational expectation and parentalinvolvement, and how the results compare to findings in the US literature. The results show somesimilarities and differences between the family-related factors that impact achievement in the USand China.

Educational Expectations of Parents and Children: Findings froma Case of China

Jing Guo 한국사회복지학회 2014 Asian Social Work and Policy Review Vol.8 No.3

To examine educational expectations and the factors that influence them, this paper presents a study of elementary school-aged children in China, with a particular interest in migrant children. This study revealed the significance of family socioeconomic status (SES), parental involvement, and children’s school experiences to children’s educational expectations. The findings of this study showed no significantly different patterns of factors influencing children’s educational expectations between the migrant and the non-migrant children in public schools. The implication of this finding is that it is not simply migrant status which affects a child’s educational expectation; rather it is a set of family SES and school experiences. Future research considering diverse migrant child population is suggested.

Function of Oct4 in mammalian embryonic development

Jing Guo,Jae-Dal Lee,Xiang-Shun Cui 충북대학교 동물생명과학연구소 2015 동물생명과학연구 Vol.7 No.-

Oct4 is a member of the OCT protein family.The Oct-4gene encodes a transcription factor that isspecifically expressed in embryonic stem cells and germ cells ofthe mouse embryo. The key role of Oct4 in the pluripotency regulator network is well documented. The Oct4 has been shown as a master regulator in maintaining the pluripotecy and self?renewal in embryonic stem cells (ESCs), but it’s not essential for the initiation of totipotecy and pluripotecy. This conclusion is further supported by the formation ofOct4 -GFP ? and Nanog- expressing inner cellmasses (ICMs) in embryos with complete inactivation of both maternal and zygotic Oct4 express ion and thereprogramming of fibroblasts into fully pluripotent cells by Oct4 -deficient oocytes. In addition, Oct4 is also an important component in generating iPS cells. In contrast to its critical role in pluripotency, maternal Oct4 expression is not critical for developmental competence of the oocyte. In this review, we summarize the function of Oct4 in mammalian embryonic development.