Developmental changes in cell proliferation and apoptosis in the normal duck bursa of Fabricius

Jing Fang,Xi Peng 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.4

The aim of this work was to investigate developmental changesin cell proliferation and apoptosis in normal duck bursa ofFabricius using flow cytometry and immunohistochemistry. Studies were carried out on Tianfu ducks on days 24 and 27 ofembryogenesis (E24 and E27) along with days 20, 70, and 200 ofpostnatal development (P20, P70, and P200). Results showedthat the percentage of G0/G1 bursa cells significantly increasedbetween E24 and P200 while the percentage of cells in the Sphase or G2 + M phase as well as the proliferating indexobviously decreased during the same period. Proliferation cellnuclear antigen was detected in lymphocyte and interfollicularepithelium. The proliferative lymphocyte density tended todecrease from E24 to P200. Apoptotic bodies in macrophages,free apoptotic bodies, or nuclei with condensed chromatin inlymphocytes in follicles were identified by transferase-mediateddUTP nick-end labeling. Both flow cytometry and microscopicanalysis reveal that the proportion of apoptotic cells andapoptotic lymphocyte density increased from E24 to P20, fell onP70, then rose again on P200. Our foundings demonstrate thatcell proliferation decreases and apoptosis increases with age. These changes may account for duck bursa development and involution.

Punicalagin Reversed the Hepatic Injury of Tetrachloromethane by Antioxidation and Enhancement of Autophagy

Jingfang Luo,Yi Long,Guofeng Ren,Yahui Zhang,Jihua Chen,Ruixue Huang,Lina Yang 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.12

Hepatic injury is significant in the pathogenesis and development of many types of liver diseases. Punicalagin (PU) is a bioactive antioxidant polyphenol found in pomegranates. To explore its protective effect against carbon tetrachloride (CCl4)-induced liver injury and the mechanism, Institute of Cancer Research (ICR) mice and L02 cells were used to observe the changes of serum biochemical indicators, histopathological liver structure, cell viability, antioxidative indices, and autophagy-related proteins were assessed. In ICR mice, PU ameliorated the CCl4-induced increase of the serum aspartate aminotransferase, alanine aminotransferase, the activity of liver lactate dehydrogenase, and the damage of histopathological structure, and exhibited a hepatoprotective effect against CCl4. PU attenuated oxidative stress by decreasing the liver malondialdehyde level and increasing the activities of liver superoxide dismutase, glutathione peroxidase, and the expression of the liver nuclear factor E2-related factor (Nrf2) protein. Furthermore, according to the vivo and vitro experiments, PU might activate autophagy through the mediation of the Akt/FOXO3a and P62/Nrf2 signaling pathway. Taken together, these results suggest that PU may protect against CCl4-induced liver injury through the upregulation of antioxidative activities and autophagy.

Identification of MKI67, TPR and TCHH mutations as prognostic biomarkers for patients with defective mismatch repair in stage II/III colon cancer

Jingfang Lv,Wenbin Li,Lei Guo,Xintong Wang,Dongliang Wang,Beifang Niu,Xishan Wang,Zheng Liu 대한종양외과학회 2022 대한임상종양학회 학술대회지 Vol.2022 No.2

Effects of Co-60 radiation on the activities of three main antioxidant enzymes in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Pumo Cai,Jingfang Hong,Cong Wang,Yanchuan Yang,Chuandong Yi,Jiahua Chen,Qinge Ji 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

Co-60 radiation has been broadly used for pest management because it results in male sterility and a lack ofemergence of unparasitized hosts due to oxidative damage. Insect life is significantly affected by abiotic stressfactors, including Co-60 radiation. In the present study, we determined the influence of Co-60 radiation on animportant pest in southern China, the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae). We exposed 30-h-old B. dorsalis eggs to Co-60 radiation at doses of (0, 5, 10, 15, 20, 25, 30, 35, and 40 Gy) and determined theactivity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX). CAT andPOX activity significantly increased in B. dorsalis in the 1st larvae instar after high-intensity radiation doses(25–40 Gy) and played an important role in the antioxidant response to intensive radiation. The activity of POXin B. dorsalis at the 2nd larval instar sharply decreased after different doses of radiation, except for 10 and 40 Gy. Our results demonstrated that Co-60 radiation affected the activity of antioxidant enzymes and disturbed thephysiology of B. dorsalis, especially at early stages (the 1st, 2nd larvae instar). These three antioxidant defenseenzymes cooperatively play an important role in protecting B. dorsalis from oxidative damage.

Fast Regulation Method for Commutation Shifts for Sensorless Brushless DC Motors

Xuliang Yao,Jicheng Zhao,Jingfang Wang 전력전자학회 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.5

Sensorless brushless DC (BLDC) motor drive systems are often subjected to inaccurate commutation signals and can produce high current peaks and conduction consumption. To achieve accurate commutation, a fast commutation shift regulation method for sensorless BLDC motor drive systems considering the influence of the inductance freewheeling process is presented to compensate inaccurate commutation signals. The regulation method is effective in both steady speed and variable speed operations. In the proposed method, the commutation error is gained from the line-voltage difference integral in a 60 electricaldegree conduction period and the outgoing phase current before commutation. In addition, the detection precision of the commutation error is improved due to the consideration of the freewheeling period. The commutation error is directly obtained, which avoids successive optimization and accelerates the convergence rate of the proposed method. Moreover, the commutation error features a positive or negative sign, which can be utilized as an indicator of advanced or delayed commutation. Finally, experiments are conducted to validate the effectiveness and feasibility of the proposed method. The results obtained show that the proposed method can accurately regulate commutation signals.

SEMI-IMPLICIT PRECONDITIONING TECHNIQUES FOR KRYLOV DEFERRED CORRECTION METHODS

Sun young Bu,Jingfang Huang,Michael Minion 한국산업응용수학회 2008 한국산업응용수학회 학술대회 논문집 Vol.4 No.3

We improve the efficiency of the Krylov Deferred Correction (KDC) methods by introducing the semi-implicit preconditioning techniques. The stiff component of the ODE/DAE system is solved by implicit schemes and the nonstiff parts by explicit ones in each deferred correction iteration. Compared with the fully implicit KDC techniques, the SI-KDC techniques improve the efficiency for the same accuracy and stability requirements. The analyses are validated by numerical results.

Fast Regulation Method for Commutation Shifts for Sensorless Brushless DC Motors

Yao, Xuliang,Zhao, Jicheng,Wang, Jingfang The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.5

Sensorless brushless DC (BLDC) motor drive systems are often subjected to inaccurate commutation signals and can produce high current peaks and conduction consumption. To achieve accurate commutation, a fast commutation shift regulation method for sensorless BLDC motor drive systems considering the influence of the inductance freewheeling process is presented to compensate inaccurate commutation signals. The regulation method is effective in both steady speed and variable speed operations. In the proposed method, the commutation error is gained from the line-voltage difference integral in a 60 electrical-degree conduction period and the outgoing phase current before commutation. In addition, the detection precision of the commutation error is improved due to the consideration of the freewheeling period. The commutation error is directly obtained, which avoids successive optimization and accelerates the convergence rate of the proposed method. Moreover, the commutation error features a positive or negative sign, which can be utilized as an indicator of advanced or delayed commutation. Finally, experiments are conducted to validate the effectiveness and feasibility of the proposed method. The results obtained show that the proposed method can accurately regulate commutation signals.

외국인 관광객의 구매의도에 영향을 미치는 호텔의 정서적ㆍ물리적 이미지 연구

정지은(Jeong, JiEun),장경방(Zhang Jingfang),김정아(Kim, ChungAh) 한국관광레저학회 2015 관광레저연구 Vol.27 No.11

CircBRMS1L Participates in Lipopolysaccharide-induced Chondrocyte Injury via the TLR4/NF-κB Pathway through Serving as a miR-142-5p Decoy

Ronghua Wang,Lianju Li,Jingxu Wang,Xiangzhuo Zhao,Jingfang Shen 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.1

Osteoarthritis (OA) is the main cause of pain and disability in the elderly. It is reported that circular RNA BRMS1L (BRMS1 like transcriptional repressor) (circBRMS1L) is upregulated in the synovial fluid of OA patients. Nevertheless, the biological function of circBRMS1L in OA has not been validated. Lipopolysaccharide (LPS)- induced chondrocytes (C28/I2) were used as OA cell models in vitro. Expression patterns of circBRMS1L, microRNA (miR)-142-5p, and Toll-like Receptor 4 (TLR4) mRNA were verified by quantitative real-time polymerase chain reaction. Loss-of-function experiments were performed to explore the influence of circBRMS1L on LPS-induced extracellular matrix (ECM) degradation, apoptosis, and inflammation in C28/I2 cells. Protein levels were detected by western blotting. The regulatory mechanism of circBRMS1L in OA was explored by bioinformatics analysis, dualluciferase reporter, RNA immunoprecipitation, and/or RNA pull-down assays. CircBRMS1L was prominently upregulated in OA cartilage tissues and LPS-stimulated C28/I2 cells. Inhibition of circBRMS1L reduced LPS-induced ECM degradation, apoptosis, inflammation, and oxidative stress in C28/I2 cells. MiR-142-5p was downregulated while TLR4 was upregulated in OA cartilage tissues and LPS-stimulated C28/I2 cells. CircBRMS1L adsorbed miR- 142-5p to regulate TLR4 expression. MiR-142-5p inhibitor reversed circBRMS1L silencing-mediated effects on LPSinduced ECM degradation, apoptosis, inflammation, and oxidative stress in C28/I2 cells. TLR4 elevation overturned the inhibitory influence of miR-142-5p mimic on LPSinduced ECM degradation, apoptosis, inflammation, and oxidative stress in C28/I2 cells. Notably, circBRMS1L activated the nuclear factor-κB (NF-κB) pathway by the miR-142-5p/TLR4 axis. LPS-induced ECM degradation, apoptosis, inflammation, and oxidative stress in chondrocytes were at least partially regulated by the circBRMS1L/miR- 142-5p/TLR4/NF-κB pathway, highlighting a new mechanism responsible for OA advancement.