Social Network Analysis on Interactive Behavior of Physical Education Teachers: A Case Study of Hunan Teacher Workshop

( Jingen Tang ),( Huijian Deng ),( Shijian Cao ),( Yi Xie ),( Lei Wang ) 아시아스포츠융합과학회 2021 Asia Pacific Journal of Applied Sport Sciences Vol.2 No.1

PURPOSE The purpose of this study was to improve the training efficiency of physical education teachers' workshops and realize the improvement of teachers' professional skills by discovering the structural characteristics of the interactive behaviors and semantic comments of the training members of physical education (PE) teacher workshop. METHOD Based on the social network analysis, Ucinet6.5 was used to analyze the basic attributes, betweenness centrality, core-periphery structure, block model structure, and semantic high-frequency vocabulary network structure characteristics of the members of the PE teacher workshop. RESULT The results showed that the cohesion, transmissibility and reciprocity were low; the interactive resource control of members was extremely unbalanced; core members account for 37.12%, whose modules had less interaction and lack reciprocity; the comments of core members were related to the content of physical education teaching and were significantly different from those of marginal members. CONCLUSION The network members of PE teacher workshop lacked active sharing consciousness; the core members did not pay enough attention. We should focus on exerting the influence of core members, avoid the influence of high dropout rate and insufficient interaction, and promote the sustainable development of research.

Identification of differentially expressed genes in longissimus dorsi muscle between Wei and Yorkshire pigs using RNA sequencing

Jingen Xu,Chonglong Wang,Erhui Jin,Youfang Gu,Shenghe Li,Qinggang Li 한국유전학회 2018 Genes & Genomics Vol.40 No.4

Intramuscular fat (IMF) content is an important trait closely related to meat quality, which is highly variable among pig breeds from diverse genetic backgrounds. High-throughput sequencing has become a powerful technique for analyzing the whole transcription profiles of organisms. In order to elucidate the molecular mechanism underlying porcine meat quality, we adopted RNA sequencing to detect transcriptome in the longissimus dorsi muscle of Wei pigs (a Chinese indigenous breed) and Yorkshire pigs (a Western lean-type breed) with different IMF content. For the Wei and Yorkshire pig libraries, over 57 and 64 million clean reads were generated by transcriptome sequencing, respectively. A total of 717 differentially expressed genes (DEGs) were identified in our study (false discovery rate < 0.05 and fold change > 2), with 323 up-regulated and 394 down-regulated genes in Wei pigs compared with Yorkshire pigs. Gene Ontology analysis showed that DEGs significantly related to skeletal muscle cell differentiation, phospholipid catabolic process, and extracellular matrix structural constituent. Pathway analysis revealed that DEGs were involved in fatty acid metabolism, steroid biosynthesis, glycerophospholipid metabolism, and protein digestion and absorption. Quantitative real time PCR confirmed the differential expression of 11 selected DEGs in both pig breeds. The results provide useful information to investigate the transcriptional profiling in skeletal muscle of different pig breeds with divergent phenotypes, and several DEGs can be taken as functional candidate genes related to lipid metabolism (ACSL1, FABP3, UCP3 and PDK4) and skeletal muscle development (ASB2, MSTN, ANKRD1 and ANKRD2).

Association of the Porcine Cluster of Differentiation 4 Gene with T Lymphocyte Subpopulations and Its Expression in Immune Tissues

Xu, Jingen,Liu, Yang,Fu, Weixuan,Wang, Jiying,Wang, Wenwen,Wang, Haifei,Liu, Jianfeng,Ding, Xiangdong,Zhang, Qin Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.4

Cluster of differentiation 4 (CD4) is mainly expressed on $CD4^+$ T cells, which plays an important role in immune response. The aim of this study was to detect the association between polymorphisms of the CD4 gene and T lymphocyte subpopulations in pigs, and to investigate the effects of genetic variation on the CD4 gene expression level in immune tissues. Five missense mutations in the CD4 gene were identified using DNA pooling sequencing assays, and two main haplotypes (CCTCC and AGCTG) in strong linkage disequilibrium (with frequencies of 50.26% and 46.34%, respectively) were detected in the population of Large White pigs. Our results indicated that the five SNPs and the two haplotypes were significantly associated with the proportions of $CD4^-CD8^-$, $CD4^+CD8^+$, $CD4^+CD8^-$, $CD4^+$ and $CD4^+/CD8^+$ in peripheral blood (p<0.05). Gene expression analysis showed the mRNA level of the CD4 gene in thymus was significantly higher than that in lymph node and spleen (p<0.05). However, no significant difference was observed between animals with CCTCC/CCTCC genotype and animals with AGCTG/AGCTG genotype in the three immune tissues (p>0.05). These results indicate that the CD4 gene may influence T lymphocyte subpopulations and can be considered as a candidate gene affecting immunity in pigs.

Rock mechanics and wellbore stability in Dongfang 1-1 Gas Field in South China Sea

Yan, Chuanliang,Deng, Jingen,Cheng, Yuanfang,Yan, Xinjiang,Yuan, Junliang,Deng, Fucheng Techno-Press 2017 Geomechanics & engineering Vol.12 No.3

Thermal effect has great influence on wellbore stability in Dongfang 1-1 (DF 1-1) gas field, a reservoir with high-temperature and high-pressure. In order to analyze the wellbore stability in DF1-1 gas field, the variation of temperature field after drilling was analyzed. In addition, the effect of temperature changing on formation strength and the thermal expansion coefficients of formation were tested. On this basis, a wellbore stability model considering thermal effect was developed and the thermal effect on fracture pressure and collapse pressure was analyzed. One of the main challenges in this gas field is the decreasing temperature of the wellbore will reduce fracture pressure substantially, resulting in the drilling fluid leakage. If the drilling fluid density was reduced, kick or blowout may happen. Therefore, the key of safe drilling in DF1-1 gas field is to predict the fracture pressure accurately.

Tissues Expression, Polymorphisms Identification of FcRn Gene and Its Relationship with Serum Classical Swine Fever Virus Antibody Level in Pigs

Liu, Yang,Wang, Chonglong,Liu, Zhengzhu,Xu, Jingen,Fu, Weixuan,Wang, Wenwen,Ding, Xiangdong,Liu, Jianfeng,Zhang, Qin Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.8

Neonatal Fc receptor (FcRn) gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G (IgG) and is responsible for IgG transport and stabilization. In this report, the 8,900 bp porcine FcRn genomic DNA structure was identified and putative FcRn protein included 356 amino acids. Alignment and phylogenetic analysis of the porcine FcRn amino acid sequences with their homologies of other species showed high identity. Tissues expression of FcRn mRNA was detected by real time quantitative polymerase chain reaction (Q-PCR), the results revealed FcRn expressed widely in ten analyzed tissues. One single nucleotide polymorphism (SNP) (HQ026019:g.8526 C>T) in exon6 region of porcine FcRn gene was demonstrated by DNA sequencing analysis. A further analysis of SNP genotypes associated with serum Classical Swine Fever Virus antibody (anti-CSFV) concentration was performed in three pig populations including Large White, Landrace and Songliao Black pig (a Chinese indigenous breed). Our results of statistical analysis showed that the SNP had a highly significant association with the level of anti-CSFV antibody (At d 20; At d 35) in serum (p = 0.008; p = 0.0001). Investigation of expression and polymorphisms of the porcine FcRn gene will help us in further understanding the molecular basis of the antibody regulation pathway in the porcine immune response. All these results indicate that FcRn gene might be regarded as a molecular marker for genetic selection of anti-CSFV antibody level in pig disease resistance breeding programmes.