Identification of the DNA Binding Element of the Human ZNF333 Protein

Jing, Zhe,Liu, Yaping,Dong, Min,Hu, Shaoyi,Huang, Shangzhi Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.6

ZNF 333 is a new and sole gene containing two KRAB domains which has been identified currently. It is a member of subfamilies of zinc finger gene complex which had been localized on chromosome 19p13.1. The ZNF333 gene mainly encodes a 75.5 kDa protein which contains 10 zinc finger domains. Using the methods of random oligonucleotide selection assay, electromobility gel shift assay and luciferase activity assay, we found that ZNF333 recognized the specific DNA core binding sequence ATAAT. Moreover, these data indicated that the KRAB domain of ZNF333 really has the ability of transcriptional repression.

Identification of the DNA Binding Element of the Human ZNF333 Protein

( Zhe Jing ),( Ya Ping Liu ),( Min Dong ),( Shao Yi Hu ),( Shang Zhi Huang ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.6

ZNF 333 is a new and sole gene containing two KRAB domains which has been identified currently. It is a member of subfamilies of zinc finger gene complex which had been localized on chromosome 19p13.1. The ZNF333 gene mainly encodes a 75.5 kDa protein which contains 10 zinc finger domains. Using the methods of random oligonucleotide selection assay, electromobility gel shift assay and luciferase activity assay, we found that ZNF333 recognized the specific DNA core binding sequence ATAAT. Moreover, these data indicated that the KRAB domain of ZNF333 really has the ability of transcriptional repression.

CbCOR15, A Cold-Regulated Gene from Alpine Chorispora bungeana, Confers Cold Tolerance in Transgenic Tobacco

Jing Si,Jian-hui Wang,Li-jing Zhang,Hua Zhang,Ya-jie Liu,Li-zhe An 한국식물학회 2009 Journal of Plant Biology Vol.52 No.6

Rapid amplification of cDNA ends was performed to isolate cold-regulated CbCOR15 (EF208112) from Chorispora bungeana. This alpine species is distributed in subnival areas. Transcripts were detected in the leaves, but not the roots, of plants acclimated to cold temperatures. Expression was induced at high levels at both 4°C and −4°C. In comparing its deduced protein sequence to that of AtCOR15a (cold-regulated 15a in Arabidopsis thaliana), the N terminus had less homology than the C terminus while still containing a region analogous to the chloroplast-targeted signal peptide of AtCOR15a. We also introduced CbCOR15, with the CaMV 35S promoter, into tobacco. Second-generation (T1) plants had significantly increased tolerance to chilling, as determined by their electrolyte leakage, chlorophyll content, and relative water content. Further freezing-stress experiments showed that the tolerance of transgenic lines was significantly greater than that of the nontransgenics. Although the degree of chilling and freezing tolerance in the transgenic plants was not directly correlated with the accumulated levels of CbCOR15, we could conclude that this gene confers cold tolerance.

關于中國消費者産品購買決定的槪念模型硏究 : 중국소비자의 제품구매결정에 관한 개념모형연구

金鎭聖(Kim Jing Sung),朱京哲(Zhu Jing Zhe) 한국유통과학회 2006 한국유통과학회 학술대회 논문집 Vol.2006 No.-

A Novel Stability Criteria of a Class Nonlinear Fractional-order HIV-1 System with Multiple Delay

Zhe Zhang,Jing Zhang,Fanyong Cheng,Feng Liu 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.9

This paper mainly deduces a new stability criteria of the fractional-order HIV-1 system with delay on the basis of Wirtinger inequality, fractional-order Lyapunov method and integral mean value theorem. The Wirtinger inequality is rarely applied to stability analysis of fractional-order system. Nevertheless, this paper extends thegeneral form of the Lyapunov-krasovskii function to a novel fractional expression form by applying definitionof Caputo fractional derivative. Via the the integral mean value theorem, fractional-order Lyapunov method and Wirtinger inequality, the novel stability criteria is deduced. It is the integral mean value theorem that reduces the conservatism of the stability criteria. The simulation results show that the proposed criteria can satisfy different fractional-order operators.In addition, it can not only solve the stability problem of fractional-order HIV-1 system with the constant time delay, but also of the fractional-order HIV-1 system with time-varying time delay. Thus, the new stability criteria has generality and universality. So as to verify our theoretical results, many numerical simulations are provided.

Pilot Symbol Assisted Weighted Data Fusion Scheme for Uplink Base-Station Cooperation System

( Zhe Zhang ),( Jing Yang ),( Jiankang Zhang ),( Xiaomin Mu ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.2

Base Station Cooperation (BSC) has been a promising technique for combating the Inter-Cell Interference (ICI) by exchanging information through a high-speed optical fiber back-haul to increase the diversity gain. In this paper, we propose a novel pilot symbol assisted data fusion scheme for distributed Uplink BSC (UBSC) based on Differential Evolution (DE) algorithm. Furthermore, the proposed scheme exploits the pre-defined pilot symbols as the sample of transmitted symbols to constitute a sub-optimal Weight Calculation (WC) model. To circumvent the non-linear programming problem of the proposed sub-optimal model, DE algorithm is employed for searching the proper fusion weights. Compared with the existing equal weights based soft combining scheme, the proposed scheme can adaptively adjust the fusion weights according to the accuracy of cooperative information, which remains the relatively low computational complexity and back-haul traffic. Performance analysis and simulation results show that, the proposed scheme can significantly improve the system performance with the pilot settings of the existing standards.

A Novel Stability Criterion of Time-varying delay Fractional-order Financial Systems Based a New Functional Transformation Lemma

Zhe Zhang,Jing Zhang,Fanyong Cheng,Feng Liu 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.4

This paper puts forward a novel stability criterion of all cases of the time-delay fractional-order financialsystems(FFS) including FFS without time delay, FFS with constant time delay and FFS with time-varying delay. This novel stability criterion is mainly based on a new stability judgment method which contains the deductionof Wirtinger inequality, Integral mean value theorem, fractional-order Lyapunov method, and a new functionaltransformation lemma which we deduced. This new functional transformation lemma simplifies the structure ofthe novel stability criterion with fewer constraints. Thus, compared with the previous stability criterion of FFS, thenovel stability criterion of FFS has clearer structure and lower conservatism. Moreover, the novel stability criterionof FFS can also satisfy all fractional-order operators from 0 to 1. Last but not least, some numerical simulationexamples are provided to verify the effectiveness and the benefit of the proposed novel stability criterion of FFS.

A novel transmissibility concept based on wavelet transform for structural damage detection

Zhe Fan,Xin Feng,Jing Zhou 국제구조공학회 2013 Smart Structures and Systems, An International Jou Vol.12 No.3

A novel concept of transmissibility based on a wavelet transform for structural damage detection is presented in this paper. The main objective of the research was the development of a method for detecting slight damage at the incipient stage. As a vibration-based approach, the concept of transmissibility has attracted considerable interest because of its advantages and effectiveness in damage detection. However, like other vibration-based methods, transmissibility-based approaches suffer from insensitivity to slight local damage because of the regularity of the traditional Fourier transform. Therefore, the powerful signal processing techniques must be found to solve this problem. Wavelet transform that is able to capture subtle information in measured signals has received extensive attention in the field of damage detection in recent decades. In this paper, we first propose a novel transmissibility concept based on the wavelet transform. Outlier analysis was adopted to construct a damage detection algorithm with wavelet-based transmissibility. The feasibility of the proposed method was numerically investigated with a typical six-degrees-of-freedom spring-mass system, and comparative investigations were performed with a conventional transmissibility approach. The results demonstrate that the proposed transmissibility is more sensitive than conventional transmissibility, and the former is a promising tool for structural damage detection at the incipient stage.

Development of a Novel 30 kV Solid-state Switch for Damped Oscillating Voltage Testing System

Zhe Hou,Hongjie Li,Jing Li,Shengchang Ji,Chenxi Huang 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.2

This paper describes the design and development of a novel semiconductor-based solid-state switch for damped oscillating voltage test system. The proposed switch is configured as two identical series-connected switch stacks, each of which comprising 10 series-connected IGBT function units. Each unit consists of one IGBT, a gate driver, and an auxiliary voltage sharing circuit. A single switch stack can block 20 kV-rated high voltage, and two stacks in series are proven applicable to 30 kV-rated high voltage. The turn-on speed of the switch is approximately 250 ns. A flyback topology-based power supply system with a front-end power factor correction is built for the drive circuit by loosely inductively coupling each unit with a ferrite core to the primary side of a power generator to obtain the advantages of galvanic isolation and compact size. After the simulation, measurement, and estimation of the parasitic effect on the gate driver, a prototype is assembled and tested under different operating regimes. Experimental results are presented to demonstrate the performance of the developed prototype.

Numerical simulation of magnetic fluid hyperthermia based on multiphysics coupling and recommendation on preferable treatment conditions

Jing Li,Huan Yao,Yan Lei,Weihua Huang,Zhe Wang 한국물리학회 2019 Current Applied Physics Vol.19 No.9

In this study, we established a multiphysics coupling model of magnetic fluid hyperthermia (MFH), using complex magnetic permeability to solve the magnetic losses of magnetic nanoparticles (MNPs). The experiments were performed to verify the validity of numerical coupling method. The optimal treatment time (OTT) was regarded as the time required for the lowest temperature point of the tumor to attain the damage criteria. The OTT increased by about 42 s as the tumor radius increased by 1 cm, and decreased by 10 s for the increase in MNP dose per gram of tumor by 1 mg. To achieve cost-effective therapies under moderate treatment conditions, the preferable ranges of external magnetic field intensity H0 and frequency f, MNP radius R and volume fraction ϕ are 3–11 kA/m, 200–500 kHz, 8–10 nm, and 5%–10%, respectively. It is greatly encouraged to adopt the combination of higher H0 (8–11 kA/m) and lower f (200–300 kHz), and the conjunction of higher R and ϕ. There was a slight thermal damage to normal tissues due to eddy current loss. In conclusion, MFH can provide an excellent therapeutic effect for deep tumors.