The expression patterns of RANKL and OPG in murine tooth eruption

Hwang, Kyung-Mun,Kim, Eun-Jung,Kim, Hyun-Jung,,Kim, Young-Jin,Nam, Soon-Hyeun 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

치아의 맹출은 치아기 (dental organ)와 치조골의 세포와 연관된 매우 복잡한 과정이다. 우선 치아 맹출이 일어나기 전에 파골세포가 치낭으로 집결하게 된다. 이러한 치낭의 역할은 파골세포와 조골세포의 상호작용으로 이루어 지는 골개조와 밀접한 관련이 있는데, 이는 치아 맹출과 연관된 많은 유전자들이 치낭에서 발현되기 때문이다. RANKL는 TNF ligand family로써 조골세포에 존재하며 파골세포의 형성 및 전구세포로 부터의 활성화를 유도한다. 이러한 RANKL은 OPG에 의해 그 작용이 억제되며 RANKL와 OPG의 상대적인 비율이 파골세포의 형성에 영향을 미친다. 또한 Runx2유전자의 변이는 조골세포의 분화와 활성에 차질을 가져오고 결국 RANKL/OPG pathway를 통해 파골세포 형성에 영향을 줄 수 있다. 치아의 발육 및 맹출에 미치는 RANKL 및 OPG의 영향을 알아보고 Runx2와의 연관성을 알아보기 위해 in situ hybridization 방법으로 태생 1, 3, 5, 7, 9, 11일된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시한 결과 RANKL, OPG, Runx2의 mRNA가 태생 1일부터 11일까지 치낭 및 치아주위조직에 특성있게 나타났다. 이중 태생 5일에서 9일 사이에 RANKL 및 Runx2는 치아의 교합면측과 하방 치조골 부위의 발현이 강하게 나타난 반면 OPG는 약한 발현을 보였다. 이는 또한 파골세포의 활성부위를 알아보기 위해 TRAP염색을 실시하여 태생 5일에서 9일 사이에 최대의 활성화를 나타낸 결과와 연광성 있게 나타났다. RANKL, OPG, Runx2의 특성있는 발현양상들을 종합해 볼 때, 치아 맹출은 치낭, 치아기, 치조골 사이의 상호 작용을 통해 이루어 지며, 이는 치낭이 치아 맹출에 있어서 매우 중요하다는 것을 의미한다. 또한, 이러한 유전자들 (RANKL, OPG, Runx2) 이 치아의 맹출에 중요한 역할을 하는 것으로 사료된다. Tooth eruption is a complex and tightly regulated process that involves cells of the tooth organ and the surrounding alveolus. Osteoclast precursors must be recruited into the dental follicle prior to the onset of eruption. This function of dental follicle may be regarded as the ability of bone remodeling characterized by the interaction of osteoclasts and osteoblasts. This is because tooth eruption is a localized event in which many of the genes required for eruption are expressed in the dental follicle. RANKL is a membrane-bound protein that is a member of the TNF ligand family, which is present on bone marrow stromal cells and osteoblasts, and induces osteoclast formation and activation from precursor cell. The biologic effect of RANKL is inhibited by OPG and, in bone, the relative ratio of RANKL and OPG modulates osteoclastogenesis. To evaluate the roles of RANKL and OPG in tooth eruption and the relations with the expression pattern of Runx2, in situ hybridization was performed with mandibles of mice at postnatal stage 1, 3, 5, 7, 9 and 11. mRNA of RANKL, OPG, and Runx2 are expressed in dental follicle and surrounding tissue from P1 to 11. To determine the sites of osteoclastic activity duing tooth eruption, mandibles were dissected. Peak osteoclastic activity in alveolar bone along the occlusal and basal regions was observed from P5 to 9, with osteoclasts in these regions being large and strongly TRAP-positive. The specific spatic-temporal expression patterns of RANKL, OPG and Runx2 in our study suggest that tooth eruption could be progressed through the interactions of molecular signaling among dental follicle, dental organ and alveolar bone, furthermore it means that dental follicle is quite important in tooth eruption. In addition, it indicates that these genes (RANKL, OPG, and Runx2) play critical roles in tooth eruption.

E형 간염과 동반된 핍뇨성 급성신부전1예

최정문,이진성,이상호 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

E형 간염은 수인성 바이러스 간염으로 산발적으로 발생되며, 이차적인 사람간 전파는 없다. 진단 방법은 HEV Ab IgM 이나 PCR로 혈청이나 분변에서 HEV genome을 발견한다. 1~4% 에서는 급성 간부전이 생기며 임산부나 이전에 간 질환이 있었던 환자에게는 전격성 간염이 생길 가능성이 높다. 현재, 국내에서 E형간염에 대한 사례보고가 있다, 그러나 E형간염에서 급성신부전을 동반한 증례는 없었다. 해외에서는 비핍뇨성 급성신부전과 동반된 E형간염이 보고된 적이 있다. 저자들은 E형 간염과 관련된 핍뇨성 급성신부전 환자 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다 Hepatitis E is water-borne viral hepatitis similar to hepatitis A and an endemic disease. Most cases of hepatitis E in industrialized countries are described in immigrants and visitors of endemic areas or are thought to be spread zoonotically(commonly swine). Hepatitis E is self-limited. But it can be followed by acute liver failure. Fulminant hepatitis is more likely in those who are pregnant or have preexisting liver disease. The diagnosis of hepatitis E is based upon the detection of its genome in serum or feces by PCR or the antibody(IgM) of hepatitis E virus. We report a case of oliguric acute renal failure associated with hepatitis E.

다량의 균일한 액적 제조를 위한 분리막 기반 미세유체 소자 시스템

장문배, 유진오, 염경호 충북대학교 산업과학기술연구소 2013 산업과학기술연구 논문집 Vol.27 No.2

In this study, we designed the microfluidic device system based on the separation membrane for generating monodisperse emulsion droplets with diameters ranging from hundreds of nanometers to several micrometers. To generate fine droplets, T-junction and flow-focusing geometry are integrated into the microfluidic channel. Also put the membrane into the microfluidic device to make droplets effectively. PDMS devices can be easily, quickly, and inexpensively fabricated with superb resolution. However, PDMS devices are swelled other organic solvent. So we could produce droplets using fluorocarbon oil. The droplet size was controlled by adjusting the flow ratio between continuous phase flow and dispersed phase flow and also membrane pore size

대학교양체육의 운영 실태분석

진윤수,정문현,이종길 忠南大學校體育科學硏究所 1996 體育科學硏究誌 Vol.14 No.1

The purpose of this study was to analyze the present condition of college physical education the survey was carried out with thirty seven national or private colleges the findings of this study could be summarized as followings; 1. College physical educaion was largely devided into three fields-physical education, art education, and education transforted from required subject. The most widely carried field was that of art education. 2. Various opportunities for college physical education were given to students, and there were thirteen col-leges where more than ten subjects were taken. Especially in Seoul National University, forty subjects were tak-en. But the number of colleges where less than four subjects was sixteen. 3. Twenty two colleges took subjects concerned with high-class sports such as leisure, recreation, golf, ski and so on. The subjects were composed to make students go through high-class sports so they are suppposed to strengthen students' abilities to adapt themselves to modern society. 4. To take a unit, students could freely choose subjects among three diffrent fields of college physical edu-cation. And the larger part of students took one or two subjects.

고양이 망막에서 Substance P성 신경원의 연접구조에 대한 면역세포화학적 연구

이문용,천명훈,한승호,정진웅 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.1

삶의 질 향상을 위한 가정체육 실태조사

진윤수,김일곤,정문현 忠南大學校體育科學硏究所 1997 體育科學硏究誌 Vol.15 No.1

The research on the condition of family's health and physical exercise was made by three thousand families in Thaejon and in Chungnam. In the questionnaire, there were questions about health condition, existing physical exercises at home, quality of life, and a developmental plan for domestic exercises. And the report runs as follows. 1. In most of families, they feel happy. They believe that the most important thing to decide their happiness is their job. So they consider working more importantly than leisure for family happiness. 2. The health conditions of families are good. The bad factors that injure their health were fatness, irregular habit, ill balanced nutrition, and lack of exercise. They are taking exercise for health of family. 3. They keep on exercise to promote health. And they think that new programs of family physical education should be made continuously. As a usual, every husband in the families decides what kind of exercise to do. They spend about thirty or sixty minutes per one exercise. 4. It is necessary for husbands and wives to exercise together. They can not exercise because they don't have enough time. The couples prefer public sports facilities in the park because they are convenient to use anytime. 5. It is necessary for family to exercise together. Most of them exercise once a week at most. They prefer public sports facilities in the park because of their convenience. 6. They think that it is necessary to exercise with their neighbors. They have participated in exercise with neighbors once and less. When they exercised with neighbors, most of them used to do so in the nearest park. 7. When they exercise, most of couples play tennis. They usually go mountain climbing for family exercise. Badminton is prefered for an exercise with neighbors. 8. They want lots of family physical education programs to be developed and supplied by the support of government, And it is necessary to inspire people with new consciousness about family physical education to develop family exercise. And the financial problems should be supported by national treasury and a loc al tax.

안정화제를 사용한 회수수의 고형분량 및 방치시간에 따른 시멘트 모르터의 특성

金光華,김기정,이문환,이세현,한천구 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

This study is intended to investigate the fundamental properties of cement mortar with solid content and leaving time of recycled water that stabilizing agent is added. According to the results, fluidity and air content does not make a big difference with variation of solid content. As the leaving time of recycled water is long, fluidity and air content increases. Setting time is shortened due to fine particle with an increase of solid content and leaving time. Compressive strength and flexural strength increase with an increase of solid content, and they increase at the leaving time of 1 day, but decrease after 2days. Length change by drying shrinkage is smaller than that of plain concrete, and is almost same with variation of solid content. And it is reduced at the leaving time of 1 day, but increases after that. Therefore, it analyzed that the effect of stabilizing agent is best under condition of adding ratio of 0.15%, solid content of 3% and leaving time of 1day, and it also works upto solid content of 5% and leaving time of 2days.

흰쥐 적색핵에서의 Parvalbumin성 신경원의 분포상태와 미세구조

이문용,한동일,천명훈,정진웅 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

흰쥐 흑색질에서 Diaminobenzidine과 Benzidine Dihydrochloride를 이용한 Dopamine성및 Parvalbumin성 신경원의 이중면역표지

이문용,정진웅,천명훈 대한해부학회 1992 Anatomy & Cell Biology Vol.25 No.4

공공자본형성과 민간자본형성 간의 동태적 관계에 관한 분석

문병근,정진현 한국재정.공공경제학회 (구 한국재정학회) 2002 재정논집 Vol.16 No.2

본 연구에서는 우리 나라의 1970-2000년간의 자료를 가지고 공공자본형성과 민간 자본형성 간의 인과관계 및 장기균형관계를 분석하였다. 변수들의 안정성 검정결과는 변수들이 동일한 차수의 단위근을 가지는 것으로 나타나 불안정적인 것으로 평가되었다. 그리고 두 변수 간에는 공적분관계가 존재하는 것으로 나타나 공공자본형성과 민간자본형성 간에 적절한 장기균형관계가 존재하는 것으로 분석되었다. 장기적으로 두 변수 간에는 쌍방의 인과관계가 나타나는 것으로 분석되었고, 두 변수 간의 장기적인 균형관계는 양의 관계가 존재하는 것으로 나타났다. 그리고 장기균형으로부터의 이탈시 장기균형으로의 조정은 공공자본형성이 주도하는 것으로 분석되었다. 이러한 분석결과는 장기적으로 공공자본형성이 민간자본을 구축하기보다는 유인한다는 가설이 우리 나라의 현실에 부합한다는 것을 의미한다. This paper ananlyzes the causal relations between the public capital formation and the private capital formation in Korea over the periods 1970 to 2000. I test for causality by using ADF, Johansen co-integration and Grnger causality tests. There are usually two propositions regarding the relationship between the public capital formation and private capital formation.: the proposition which states that the public capital formation crowds out or crowds in the private capital formation and the proposition which states that as the private capital formation grows, the public capital formation tends to grow. The Empirical results suggest that there is the bilateral causal relationship between the public capital formation and the private capital formation in the long-run. And the public capital formation relates positive relationship with the private capital formation in the long-run movement.