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Differential Expression Patterns of Gangliosides in Human and Mini-pig Kidney cells
Jin-Hyoung Cho,Ji-Su Kim,Jae-Sung Ryu,Jung-Woo Jin,Hyo-Jung Yang,Eun-Jung Jeong,So-Dam Lee,So-Hyun Lee,Young-Choon Lee,Young-kug Choo 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
Ganglioside are ubiquitous membrane component in mammalian cells and suggested to play important roles in various cell functions such as cell-cell recognition, differentiation and transmembrane signaling. These compounds are localized in a glycosphingolipid-enriched microdomain on the cell surface and regulated by the glycosphingolipid composition. However, the role that gangliosides play in immune rejection response by xenotransplantation is not yet clearly understood. In this study, we differential expression patterens of gangliosides between HEK293 cells (human embryonic kidney cells) and mini-pig kidney cells (NIH-mini pig, primary cultured) was investigated. we examined expression level of high-performance thin-layer chromatography (HPTLC) showed that HEK293 cells and mini-pig kidney cells contained GM3, GM2 and GD3 as major gangliosides. Moreover, ganglioside GM3, GM2, GM1 and GD3 which are gangliosides of mini-pig kidney cells expressed more than HEK293 cells. Especially, ganglioside GT1b expressed in HEK293 cells, not in mini-pig kidney cells. and GM1 expressed in mini-pig kidney cells, not in HEK293 cells. As a results suggest that differential expression patterens of gangliosides of HEK293, mini-pig kidney cells are related immune rejection response in xenotransplantation by biomaker.
Surface Modification of Zirconia Substrate by Calcium Phosphate Particles Using Sol-Gel Method.
Jin, So Dam,Um, Sang Cheol,Lee, Jong Kook American Scientific Publishers 2015 Journal of nanoscience and nanotechnology Vol.15 No.8
<P>Surface modification with a biphasic composition of hydroxyapatite (HA) and tricalcium phosphate (TCP) was performed on a zirconia substrate using a sol-gel method. An initial calcium phosphate sol was prepared by mixing a solution of Ca(NO3)2 · 4H20 and (C2H5O)3P(O), while both porous and dense zirconia were used as substrates. The sol-gel coating was performed using a spin coater. The coated porous zirconia substrate was re-sintered at 1350 °C 2 h, while coated dense zirconia substrate was heat-treated at 750 °C 1 h. The microstructure of the resultant HA/TCP coatings was found to be dependent on the type of zirconia substrate used. With porous zirconia as a starting substrate, numerous isolated calcium phosphate particles (TCP and HA) were uniformly dispersed on the surface, and the particle size and covered area were dependent on the viscosity of the calcium phosphate sol. Conversely, when dense zirconia was used as a starting substrate, a thick film of nano-sized HA particles was obtained after heat treatment, however, substantial agglomeration and cracking was also observed.</P>
Jin, So Dam,Lee, Bo Ram,Hwang, Young Sun,Lee, Hong Jo,Rim, Jong Seop,Han, Jae Yong BioMed Central 2017 Journal of animal science and biotechnology Vol.8 No.-
<P><B>Background</B></P><P>Primordial germ cells (PGCs), the precursors of functional gametes, have distinct characteristics and exhibit several unique molecular mechanisms to maintain pluripotency and germness in comparison to somatic cells. They express germ cell-specific RNA binding proteins (RBPs) by modulating tissue-specific <I>cis</I>- and <I>trans</I>-regulatory elements. Studies on gene structures of chicken <I>vasa</I> homologue (<I>CVH</I>), a chicken RNA binding protein, involved in temporal and spatial regulation are thus important not only for understanding the molecular mechanisms that regulate germ cell fate, but also for practical applications of primordial germ cells. However, very limited studies are available on regulatory elements that control germ cell-specific expression in chicken. Therefore, we investigated the intricate regulatory mechanism(s) that governs transcriptional control of <I>CVH</I>.</P><P><B>Results</B></P><P>We constructed green fluorescence protein (GFP) or luciferase reporter vectors containing the various 5′ flanking regions of <I>CVH</I> gene. From the 5′ deletion and fragmented assays in chicken PGCs, we have identified a <I>CVH</I> promoter that locates at −316 to +275 base pair fragment with the highest luciferase activity. Additionally, we confirmed for the first time that the 5′ untranslated region (UTR) containing intron 1 is required for promoter activity of the <I>CVH</I> gene in chicken PGCs. Furthermore, using a transcription factor binding prediction, transcriptome analysis and siRNA-mediated knockdown, we have identified that a set of transcription factors play a role in the PGC-specific <I>CVH</I> gene expression.</P><P><B>Conclusions</B></P><P>These results demonstrate that <I>cis</I>-elements and transcription factors localizing in the 5′ flanking region including the 5′ UTR and an intron are important for transcriptional regulation of the <I>CVH</I> gene in chicken PGCs. Finally, this information will contribute to research studies in areas of reproductive biology, constructing of germ cell-specific synthetic promoter for tracing primordial germ cells as well as understanding the transcriptional regulation for maintaining germness in PGCs.</P>
Ganglioside GT1b Mediates Neuronal Differentiation of Mouse Embryonic Stem Cells
Lee So-Dam,Jin Jung-Woo,Choi Jin,Choo Young-Kug 한국발생생물학회 2009 발생과 생식 Vol.13 No.3
It has been reported that ganglioside GT1b is expressed during neuronal cell differentiation from undifferentiated mouse embryonic stem cells (mESCs), which suggests that ganglioside GT1b has a direct effect on neuronal cell differentiation. Therefore, this study was conducted to evaluate the effect of exogenous addition of ganglioside GT1b to an in vitro model of neuronal cell differentiation from undifferentiated mESCs. The results revealed that a significant increase in the expression of ganglioside GT1b occurred during neuronal differentiation of undifferentiated mESCs. Next, we evaluated the effect of retinoic acid (RA) on GT1b-treated undifferentiated mESCs, which was found to lead to increased neuronal differentiation. Taken together, the results of this study suggest that ganglioside GT1b plays a crucial role in neuronal differentiation of mESCs.
홍소담 ( So Dam Hong ),신재경 ( Jae Kyoung Shin ),홍희진 ( Hee Jin Hong ),허진형 ( Jin Hyung Heo ),정소영 ( So Young Chong ),오도연 ( Do Youn Oh ),오지수 ( Ji Su Oh ) 대한내과학회 2016 대한내과학회지 Vol.90 No.1
비록 자궁경부암이 갑상선으로의 전이는 매우 드물지만, 자궁경부암의 과거력이 있는 경우, 갑상선부위의 부종이나 촉지되는 결절이 발생하면 반드시 전이성 병변을 의심해봐야 한다. 비록 갑상선으로의 전이는 매우 나쁜 예후를 의미 하지만 고식적 치료로 전신항암요법 및 방사선요법이 환자의 삶의 질 개선에 도움을 줄 수 있다. 드물기는 하지만 갑상선으로의 악성전이 보고가 증가하는 추세로, 이에 대해 어떠한 치료가 가장 효과적일지에 대한 연구가 필요하겠다. Most patients with recurrent uterine cervical cancer have intra-pelvis metastasis with adjacent lymph node involvement, while a lone, distant metastasis is extremely rare. We report a 79-year-old woman with recurrent uterine cervical cancer that presented as thyroid mass with no intra-pelvic recurrence. Four years earlier, the patient had been diagnosed with uterine cervical cancer. She had undergone a course of concurrent chemoradiotherapy to the pelvis and had no subsequent evidence of recurrence. Several weeks before presenting, she had noticed a foreign body sensation in her throat and a palpable mass in the left side of her neck. Clinically, this was metastatic squamous cell carcinoma from the uterine cervix. Patients who present with swelling or palpable nodules in the neck with a previously diagnosed malignancy must be evaluated for metastatic disease, although metastasis from uterine cervical carcinoma to the thyroid gland is rare. (Korean J Med 2016;90:68-71)
Jung-Woo Jin,Jae-Sung Ryu,Hyo-Jung Yang,Jin-Hyoung Cho,Eun-Jeong Jeong,So-Dam Lee,So-Hyun Lee,Young-Choon Lee,Young-Kug Choo 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
Ganglioside GQ1b has important functions in neuronal differentiation and neuronal development processs. The regulation of ganglioside levels is relationship to the induction of neuronal cell differentiation. In the previous study, we showed that expression of GT1b and GQ1b gangliosides in undifferentiated mouse embryonic stem cell were exhibit during their neural differentiation. We evaluated the co-localization of ganglioside GQ1b and neuroanl markers in an in vitor model of neuronal differentiation in mouse embryonic stem and found that neuronal differentiation was facilitated by the presence of increased gangliosides, especially GQ1b, due to daunorubicin. Furthermore, we investigated that important effect of GQ1b by overexpression and konckdown GQ1b in the neural differentiate. First, we designed a primer of a2,8-sialyltransferase (ST8Sia V) for producing GQ1b. In this study, we inserted ST8Sia V gene to overexpression (pEGFP-C2), and knock-down (pLKO.1 puro ShRNA) vectors. We will confirm the efficient transfectionof mouse embryonic stem cell by green fluorescent protein (GFP) and expressin of ganglioside GQ1b by immunofluorescence staining analyses.