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Ji, Hong,Wang, Jianfa,Liu, Juxiong,Guo, Jingru,Wang, Zhongwei,Zhang, Xu,Guo, Li,Yang, Huanmin Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3
Zi geese (Anser anser domestica) belong to the white geese and are excellent layers with a superior feed-to-egg conversion ratio. Quantitative gene expression analysis, such as Real-time qRT-PCR, will provide a good understanding of ovarian function during egg-laying and consequently improve egg production. However, we still don't know what reference genes in geese, which show stable expression, should be used for such quantitative analysis. In order to reveal such reference genes, the stability of seven genes were tested in five tissues of Zi geese. Methodology/Principal Findings: The relative transcription levels of genes encoding hypoxanthine guanine phosphoribosyl transferase 1 (HPRT1), ${\beta}$-actin (ACTB), ${\beta}$-tubulin (TUB), glyceraldehyde-3-phosphate-dehydrogenase (GADPH), succinate dehydrogenase flavoprotein (SDH), 28S rRNA (28S) and 18S rRNA (18S) have been quantified in heart, liver, kidney, muscle and ovary in Zi geese respectively at different developmental stages (1 d, 2, 4, 6 and 8 months). The expression stability of these genes was analyzed using geNorm, NormFinder and BestKeeper software. Conclusions: The expression of 28S in heart, GAPDH in liver and ovary, ACTB in kidney and HPRT1 in muscle are the most stable genes as identified by the three different analysis methods. Thus, these genes are recommended for use as candidate reference genes to compare mRNA transcription in various developmental stages of geese.
( Ji Hong ),( Peng Zhang ),( I Na Yoon ),( Jae Sam Hwang ),( Jin Ku Kang ),( Ho Kim ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.4
Clostridium difficile, which causes pseudomembranous colitis, releases toxin A and toxin B. These toxins are considered to be the main causative agents for the disease pathogenesis, and their expression is associated with a marked increase of apoptosis in mucosal epithelial cells. Colonic epithelial cells are believed to form a physical barrier between the lumen and the submucosa, and abnormally increased mucosal epithelial cell apoptosis is considered to be an initial step in gut inflammation responses. Therefore, one approach to treating pseudomembranous colitis would be to develop agents that block the mucosal epithelial cell apoptosis caused by toxin A, thus restoring barrier function and curing inflammatory responses in the gut. We recently isolated an antimicrobial peptide, Periplanetasin-2 (Peri-2, YPCKLNLKLGKVPFH) from the American cockroach, whose extracts have shown great potential for clinical use. Here, we assessed whether Peri-2 could inhibit the cell toxicity and inflammation caused by C. difficile toxin A. Indeed, in human colonocyte HT29 cells, Peri-2 inhibited the toxin A-induced decrease in cell proliferation and ameliorated the cell apoptosis induced by this toxin. Moreover, in the toxin A-induced mouse enteritis model, Peri-2 blocked the mucosal disruption and inflammatory response caused by toxin A. These results suggest that the American cockroach peptide Peri-2 could be a possible drug candidate for addressing the pseudomembranous colitis caused by C. difficile toxin A.
A Model of Infinitely Repeated Litigation with Correlated Decisions
Jihong Lee 한국계량경제학회 2010 계량경제학보 Vol.21 No.4
This paper considers a model of repeated litigation in which a single long-lived defendant faces an infinite sequence of short-lived plaintiffs. Court decisions are correlated across periods, rejecting the presence of precedent effects. We derive unique Markov perfect equilibrium payoffs for the long-lived defendant, and compare them to the predictions of a corresponding two-period benchmark model.
Visual lock-on to invisible target for unmanned aerial vehicle
Jihong Min,Jungho Kim,Yekeun Jung,In So Kweon IET 2012 Electronics letters Vol.48 No.14
<P>Presented is a robust visual lock-on framework for an unmanned aerial vehicle (UAV) that utilises geometric relations between the UAV pose and the 3D local map defined by the positions of the target and natural landmarks. Experimental results using real datasets demonstrate the robustness of the proposed method compared to state-of-the-art visual tracking methods.</P>
Jihong Lee,남윤미 한국은행 2019 經濟分析 Vol.25 No.4
This paper studies localization effects of knowledge spillovers in Korea using U.S. patents granted over the period 1996-2015. The “sample-matching” analysis initiated by Jaffe, Trajtenberg, and Henderson (1993) is adopted. We do not find evidence of positive localization effects in Korea. In particular, controlling for the existing geographic distribution of knowledge production, the frequency of domestic citations of Korean patents is no more than the citation frequency from overseas, and the difference is decreasing within the sample period. We also examine localization effects across regions and industries, and compare Korea with Taiwan and Japan. 본 연구는 1996-2015년 기간 동안 개인이 아닌 기업이나 기관이 미국 특허청(USPTO)에 출원 및 등록한 특허자료를 사용하여 우리나라 지식전파의 지역화 효과를 분석하였다. 이를 위해 Jaffe, Trajtenberg, and Henderson (1993)이 제시한 ‘샘플-매칭(matched-sample)’ 분석틀을 활용하였으며, 지식 생산의 지리적 집적 분포를 통제한 후 국내에 있는 발명가의 특허를 국내 다른 발명가가 인용할 확률과 해외에 있는 발명가가 인용할 확률을 비교하는 방식으로 지식전파의 지역화 효과를 추정하였다. 분석결과, 우리나라 지식전파의 지역화 효과의 증거를 발견할 수 없었으며 오히려 감소하고 있는 추세를 보이고 있는 것으로 나타났다. 이는 지식 창출의 지리적 집적 정도를 통제한 후에는 국내 발명가의 특허를 타 기관이나 기업 소속의 국내 다른 발명가가 인용할 확률과 해외에 있는 발명가가 인용할 확률이 같거나 오히려 후자가 높은 것을 의미한다. 아울러 본 연구는 국내 지역별 및 산업별 지식전파의 지역화 효과와 함께, 한국의 지역화 효과를 대만과 일본의 경우와 비교분석 하였다.
Optimization of domes against instability
Jihong Ye,Mingfei Lu 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.28 No.4
Static stability is a decisive factor in the design of domes. Stability-related external factors, such as load and supports, are incorporated into structural vulnerability theory by the definition of a relative rate of joint well-formedness (<i>r<sub>r</sub></i>). Hence, the instability mechanism of domes can be revealed. To improve stability, an optimization model against instability, which takes the maximization of the lowest <i>r<sub>r</sub></i> (<i>r</i><sub><i>r</i>,min</sub>) as the objective and the discrete member sections as the variables, is established with constraints on the design requirements and steel consumption. Optimizations are performed on two real-life Kiewitt-6 model domes with a span of 23.4 m and rise of 11.7 m, which are initially constructed for shaking table collapse test. Well-formedness analyses and stability calculation (via arc-length method) of the models throughout the optimization history demonstrate that this proposed method can effectively enhance <i>r</i><sub><i>r</i>,min</sub and optimize the static stability of shell-like structures. Additionally, seismic performance of the optimum models subjected to the same earthquake as in the shaking table test is checked. The supplemental simulations prove that the optimum models are superior to the original models under earthquake load as well.