Cytotoxicity of polymethyl methacrylate cement on primary cultured metastatic spinal cells

Ji Fang,Jieliang Shen,Wei Jiang,Wen Dong,Zhenming Hu,Zhenming Hu 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.2

Polymethyl methacrylate (PMMA) bone cement has been commonly used for percutaneous injection into collapsed vertebral bodies due to malignant tumor. The purpose of this study was to investigate the possible mechanisms of PMMA’s cytotoxcity on primary cultured spinal metastastic cells (SMCs) in vitro. PMMA specimens were prepared in standard discs made of dough and polymerization stages, and the eluates were prepared following the ISO standard. Primary SMCs were obtained and isolated from 7 patients with spinal metastatic tumors undergoing vertebroplasty. Primary cultured SMCs were treated with PMMA specimens of different stages for 24 h, or co-cultured with extracted medium for successive 3 days. The temperatures in two locations from cement discs were recorded by K-type thermocouples. Furthermore, cell proliferation, apoptosis and cycles were determined by MTT and flow cytometry, respectively. The modulation of apoptotic proteins (bcl- 2, bax, caspase-3, caspase-8, Fas and PARP) and cell cycle proteins (cyclin D1, P21 and P27) were analyzed by western blot and real time PCR. The results of this study demonstrated that PMMA treatment was able to suppress SMCs proliferation, induce cell apoptosis and inhibit cell cycle arrest when compared to the control group in vitro (P<0.05). Simultaneously, the temperature recorded at the periphery of the PMMA specimen was not high enough to casue thermal injury. Furthermore, molecular markers of apoptosis including Fas, caspase-3 and caspase-9 activated, and Bcl-2/Bax dysregulated in the SMCs with PMMA stimulation. In addition, PMMA treatment showed decreased expression of cyclin D1 that induces cell cycle and increased epxression of inhibitory protein P21, with no significant difference of P27 expression. In summary, the present study confirms that PMMA cement can compromise the vitality and apoptosis of SMCs. This cytotoxic effect may be regulated by the biomarkers Fas, Bcl-2, Bax, caspase-3, caspase-9, cyclin D1 and P21, but not on account of thermal damage.

보조동력장치용 서보밸브 오리피스 면적의 칼만필터 추덩

장지팡(Jifang Zhang),김춘택(Chun-Taek Kim),정헌술(Heon-Sul Jeong) 유공압건설기계학회 2007 유공압건설기계학회 학술대회논문집 Vol.2007 No.11

A Fuzzy Evaluation Method of Power Transformer Insulation Health State Based on Multi-weight Fusion

Li Jifang,Zhang Haiyang,Wang Zhiyue,Feng Zhen 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.4

During the evaluation of transformer insulation health state, the weight of each evaluation indicator has a signifi cant infl uence on its evaluation result. To overcome the existing problem of determining the weight of evaluation indicators by a single method, this paper proposes a comprehensive weight fuzzy evaluation method based on multi-weight fusion. Firstly, the proposed method uses principal component analysis (PCA) method to determine the objective weight of each evaluation indicator based on the measured data of the power transformer. Then years of practical experiences from industry experts are introduced to subjectively study the relative importance of the evaluation indicator’s infl uences on the insulation health states and to build the relative importance of judgment matrix of relative relationship. The subjective weight of each indicator is determined by using the analytic hierarchy process. Next, the objective weight and the subjective weight are fused by using the least square method to obtain the comprehensive weight of the evaluation indicator. And insulation health values and insulation health levels of the power transformers are obtained by using fuzzy comprehensive evaluation. Finally, example analysis and comparison are carried out by using the actual operating data of the transformer. Compared with the single principal component analysis method, the correct rate of the comprehensive weighting method of the evaluation indicator proposed in this paper is improved by 5.9%, which is 10.7% higher than that of the single analytic hierarchy process. The results verify the eff ectiveness and practicability of the method proposed in this paper. The proposed method provides a scientifi c basis for the maintenance and overhaul of the transformer. It has a very important practical signifi cance for improving the stability of the power grid and ensuring the normal and orderly progress of the national economy.

High-efficiency removal of phenol and coking wastewater via photocatalysis-Fenton synergy over a Fe-g-C3N4 graphene hydrogel 3D structure

Jinshan Hu,Pengfei Zhang,Jifang Cui,Weijia An,Li Liu,Yinghua Liang,Qingbin Yang,Hongjun Yang,Wenquan Cui 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.84 No.-

To achieve rapid separation of electron-hole pairs and improve photocatalytic degradation activity, theFe-g-C3N4 reduced graphene hydrogel (rGH/Fe-g-C3N4) with a 3D network structure was fabricated viathe hydrothermal method. Using visible light irradiation, H2O2 was added to form a photocatalysis-Fenton synergy system. The results showed that the synergistic degradation rate constant of 10% rGH/Feg-C3N4 was 52% higher than that of the multiphase Fenton reaction and 1.5 times higher than that of theFe-g-C3N4. In the seventh cycle, the catalytic efficiency was still as high as 86.9%. Based on the optimizedconditions from phenol degradation, the system was further applied to coking wastewater treatment,and the degradation efficiency of TOC and COD in 60 min reached 66.3% and 68.1%, respectively. Such highand stable degradation performance was ascribed to the synergy effect of photocatalysis and Fenton. Because of the photogenerated electrons not only can promote the Fe3+/Fe2+ cycle, accelerate thedecomposition of H2O2, but also can quickly transfer to graphene and directly decompose H2O2 to formOH. Thus, a large amount ofOH were generated through the two different channels, which greatlyimprove the degradation efficiency. Furthermore, rGH/Fe-g-C3N4 can be regenerated usingfilters withoutthe need for additional complicated processing. This work provides an effective strategy for the deeptreatment of industrial wastewater.

EFFECT OF DIMENSIONS OF TEAM ATTACHMENT ON RUNNING GROUP IN SPORT EXERCISE

Ning (Chris) Chen,Jifang Dou,Xueli Wang 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

Over the past decade, the field of sport exercise in China gets increasingly popular, resulting in a nationwide exercise fashion (Schulenkorf, Sherry, & Rowe, 2016; Yu, Li, Liu, & Su, 2015). With this new emerging shift, this study tested the proposed structural model, and specifically tested the mediating effects of dimensions of sport team attachment between runners’ team satisfaction and their runner team building behaviors (Morhart, Herzog, & Tomczak, 2009). With a sample size of 301, three dimensions of team attachment, team identity, social bonding, and team expectation were found to be significant influencing runners’ in-role team building behavior and participation in the development of this runner team. Other important findings and implications were further discussed.

Opportunities and Challenges of in vitro Synthetic Biosystem for Terpenoids Production

Yang Liyang,Gong Qiang,Lv Jifang,Zhou Bangyuan,Li Guilan,Guo JianQuan 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.5

Terpenoids are a large variety of natural products with remarkable diverse biological functions, and have a wide range of applications in flavors, pharmaceuticals, biofuels, pigments, and so on. However, limited production of terpenoids from natural resources constrains their use of bulk commodity products. In vivo synthetic biosystem, harnessing living organisms to produce terpenoids, has been broadly used and in-depth reviewed for terpenoids production, which has inherent weaknesses, such as slow reaction rate, low product yield, toxic intermediates, and high separation cost. In vitro synthetic biosystem, harboring numerous enzymes and/or coenzymes assembled into an in vitro enzymatic bioreactions, can obviate part of problems associated with in vivo style. In this review, the general design of in vitro synthetic biosystem is presented with seven supporting examples: mevalonate, isoprene, limonene, pinene, farnesene, amorpha-4,11-diene and taxadiene. The efforts for the large-scale implementation of in vitro synthetic biosystem have been addressed to enzymes engineering, computational modeling and cofactors recycle. The review also discusses the challenges and solutions for the largescale implementation of in vitro synthetic biosystem around enzymes stability and cofactors recycle. This review may suggest in vitro synthetic biosystems become a realistic option for the production of diverse valuable terpenoids, even expand to other commodity chemicals.

PSMD12 promotes non-small cell lung cancer progression through activating the Nrf2/TrxR1 pathway

Lv Junqi,Ma Shengmao,Wang Xiaowen,Dang Jifang,Ma Fuchun 한국유전학회 2024 Genes & Genomics Vol.46 No.3

Background Non-small cell lung cancer (NSCLC) contributes to the vast majority of cancer-related deaths. Proteasome 26S subunit, non-ATPase 12 (PSMD12), a subunit of 26S proteasome complex, is known to play the tumor-promoting role in several types of cancer but its function in NSCLC remains elusive. Objective To explore the role and underlying mechanisms of PSMD12 in NSCLC. Methods The PSMD12 expression in human normal lung epithelial cell line (BEAS-2B) and four NSCLC cell lines (A549, NCI-H1299, NCI-H1975, Calu-1) were determined by qRT-PCR and western blot. Malignant phenotypes of NSCLC cells were detected by CCK-8, EdU staining, immunofluorescence staining for E-cadherin, flow cytometry, and Transwell assays to assess cell viability, proliferation, epithelial-mesenchymal transition (EMT), apoptosis, migration and invasion. Dual luciferase assay was used to verify the regulatory role of transcription factor on the promoter. Results We identified the upregulation of PSMD12 in NSCLC tissues based on the GEO datasets, which further verified in NSCLC and BEAS-2B cell lines. PSMD12 knockdown significantly suppressed malignant behaviors of NSCLC cells, including cell growth, invasion, and migration, while PSMD12 overexpression presented the opposite effects. Interestingly, we found that PSMD12 upregulated the tumor-promoting factor TrxR1 mRNA expression. For its potential mechanisms, we demonstrated that PSMD12 elevated transcription factor Nrf2 protein level and promoted Nrf2 nuclear translocation. And Nrf2 further increased TrxR1 promoter activity and enhanced TrxR1 transcription. Meanwhile, we proved that TrxR1 overexpression erased the inhibitory effect of PSMD12 knockdown. Conclusion PSMD12 promotes NSCLC progression by activating the Nrf2/TrxR1 pathway, providing a novel prognostic and therapeutic target for NSCLC treatment.

Efficient hematite photoanodes prepared by hydrochloric acid-treated solutions with amphiphilic graft copolymer

Park, Min Su,Walsh, Dominic,Zhang, Jifang,Kim, Jong Hak,Eslava, Salvador Elsevier 2018 Journal of Power Sources Vol.404 No.-

<P><B>Abstract</B></P> <P>Simple and low-cost approaches for the preparation of photoelectrodes are crucial to enable a transition towards a sustainable and circular economy in which sunlight energy is efficiently harnessed and used. Here, a novel and simple process is presented to prepare a sol solution that can be cast by spin coating deposition for mesoporous <I>α</I>-Fe<SUB>2</SUB>O<SUB>3</SUB> hematite water-splitting photoanodes, reaching 1.05 mA cm<SUP>−2</SUP> at 1.23 V<SUB>RHE</SUB> under 1 sun illumination. The sol solution is prepared using inexpensive commercial ∼10 nm <I>α</I>-Fe<SUB>2</SUB>O<SUB>3</SUB> hematite nanoparticles as hematite film precursor, an amphiphilic poly(vinyl chloride)-<I>graft</I>-poly(oxyethylene methacrylate) (PVC-<I>g</I>-POEM) graft copolymer as a pore template, and HCl acid as an iron oxide phase directing agent. The hydrophilic POEM side chains selectively interact with HCl-treated hematite nanoparticles allowing their dispersion. Moreover, the HCl in the sol solution disperses and dissolves the hematite nanoparticles which re-precipitate as mixed phase <I>γ</I>-FeOOH and <I>β</I>-FeOOH, leading to better performant hematite films due to finer nanostructures, a more pronounced hematite (110) plane, and a more hydroxylated surface. This work demonstrates that synergies between an amphiphilic graft copolymer, hematite nanoparticles and HCl acid can be exploited in the inexpensive spin coating technique to prepare robust, stable and promising hematite photoanodes for energy devices.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel wormlike mesoporous hematite films were prepared by graft copolymer templating. </LI> <LI> PVC-<I>g</I>-POEM was effective in adding porosity and limiting particle size in hematite. </LI> <LI> The use of HCl resulted in mixed phase <I>γ</I>-FeOOH/<I>β</I>-FeOOH, leading to better performant. </LI> <LI> A high photocurrent density (1.05 mA cm<SUP>−2</SUP> at 1.23 V<SUB>RHE</SUB>) and high stability were obtained. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Preparation, Characterization, and Microstructure of Poly(ε-caprolactone–co-2,2-dimethyltrimethylene carbonate) Using Metal-free Organocatalysts

Yan Wang,Jizhe Guo,Lifang Zhang,Junhua Bai,Jifang Niu,Lijuan Jiang,Junfei Li 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.7

We investigated the random copolymerization of ε-caprolactone and 2,2-dimethyltrimethylene carbonate [poly(CL-co-DTC)] promoted by various imidazolium-based carbenes, including 1-isopropyl-3-p-methoxyphenylimidazol-2-ylidene [Catalyst (1)], 1-isopropy-3-p-methylphenylimidazol-2-ylidene [Catalyst (2)], and 1-methyl-3-p-methoxyphenyl-imidazol-2-ylidene [Catalyst (3)], all of which are new types of unsymmetrical N-heterocyclic carbene catalysts. The reaction was carried out in the presence of benzyl alcohol (BnOH) as an initiator in tetrahydrofuran. By varying the experimental conditions and the monomer feed composition, poly(CL-co-DTC) with high yield and molecular weight was prepared using the binary system Catalyst (1)/BnOH as catalyst/initiator.1H NMR and differential scanning calorimetry (DSC) characterizations verified random structures of the polymer. GPC characterization further showed high molecular weights and narrow molecular weight distribution of the final product.

Adiponectin induced AMP-activated protein kinase impairment mediates insulin resistance in Bama mini-pig fed high-fat and high-sucrose diet

Miaomiao Niu,Lei Xiang,Yaqian Liu,Yuqiong Zhao,Jifang Yuan,Xin Dai,Hua Chen 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8

Objective: Adipose tissue is no longer considered as an inert storage organ for lipid, but instead is thought to play an active role in regulating insulin effects via secretion adipokines. However, conflicting reports have emerged regarding the effects of adipokines. In this study, we investigated the role of adipokines in glucose metabolism and insulin sensitivity in obese Bama mini-pigs. Methods: An obesity model was established in Bama mini-pigs, by feeding with high-fat and high-sucrose diet for 30 weeks. Plasma glucose and blood biochemistry levels were measured, and intravenous glucose tolerance test was performed. Adipokines, including adiponectin, interleukin-6 (IL-6), resistin and tumor necrosis factor alpha (TNF-α), and glucose-induced insulin secretion were also examined by radioimmunoassay. AMP-activated protein kinase (AMPK) phosphorylation in skeletal muscle, which is a useful insulin resistance marker, was examined by immunoblotting. Additionally, associations of AMPK phosphorylation with plasma adipokines and homeostasis model assessment of insulin resistance (HOMA-IR) index were assessed by Pearce's correlation analysis. Results: Obese pigs showed hyperglycemia, high triglycerides, and insulin resistance. Adiponectin levels were significantly decreased (p<0.05) and IL-6 amounts dramatically increased (p<0.05) in obese pigs both in serum and adipose tissue, corroborating data from obese mice and humans. However, circulating resistin and TNF-α showed no difference, while the values of TNF-α in adipose tissue were significantly higher in obese pigs, also in agreement with data from obese humans but not rodent models. Moreover, strong associations of skeletal muscle AMPK phosphorylation with plasma adiponectin and HOMA-IR index were obtained. Conclusion: AMPK impairment induced by adiponectin decrease mediates insulin resistance in high-fat and high-sucrose diet induction. In addition, Bama mini-pig has the possibility of a conformable model for human metabolic diseases.